AU2017284202B2 - Hydrophobic interaction chromatography for purification of oligonucleotides - Google Patents

Hydrophobic interaction chromatography for purification of oligonucleotides Download PDF

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Publication number
AU2017284202B2
AU2017284202B2 AU2017284202A AU2017284202A AU2017284202B2 AU 2017284202 B2 AU2017284202 B2 AU 2017284202B2 AU 2017284202 A AU2017284202 A AU 2017284202A AU 2017284202 A AU2017284202 A AU 2017284202A AU 2017284202 B2 AU2017284202 B2 AU 2017284202B2
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impurity
target oligonucleotide
product
previous
elution
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AU2017284202A1 (en
Inventor
Robert S. Gronke
Ratnesh S. Joshi
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Biogen MA Inc
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Biogen MA Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • B01D15/305—Hydrophilic interaction chromatography [HILIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AU2017284202A 2016-06-14 2017-06-13 Hydrophobic interaction chromatography for purification of oligonucleotides Active AU2017284202B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662349970P 2016-06-14 2016-06-14
US62/349,970 2016-06-14
US201762492402P 2017-05-01 2017-05-01
US62/492,402 2017-05-01
PCT/US2017/037126 WO2017218454A1 (en) 2016-06-14 2017-06-13 Hydrophobic interaction chromatography for purification of oligonucleotides

Publications (2)

Publication Number Publication Date
AU2017284202A1 AU2017284202A1 (en) 2019-01-17
AU2017284202B2 true AU2017284202B2 (en) 2021-11-04

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AU2017284202A Active AU2017284202B2 (en) 2016-06-14 2017-06-13 Hydrophobic interaction chromatography for purification of oligonucleotides

Country Status (11)

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US (3) US10947262B2 (enExample)
EP (1) EP3468981B1 (enExample)
JP (1) JP7075359B2 (enExample)
KR (1) KR102461234B1 (enExample)
CN (1) CN109641928B (enExample)
AU (1) AU2017284202B2 (enExample)
BR (1) BR112018075667B1 (enExample)
ES (1) ES2988362T3 (enExample)
IL (1) IL263639B (enExample)
MX (1) MX2018015654A (enExample)
WO (1) WO2017218454A1 (enExample)

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WO2016040748A1 (en) 2014-09-12 2016-03-17 Ionis Pharmaceuticals, Inc. Compositions and methods for detection of smn protein in a subject and treatment of a subject
AR102198A1 (es) 2014-10-09 2017-02-08 Regeneron Pharma Proceso para reducir partículas subvisibles en una formulación farmacéutica
AU2017312785B2 (en) 2016-08-16 2024-10-10 Regeneron Pharmaceuticals, Inc. Methods for quantitating individual antibodies from a mixture
KR102759512B1 (ko) 2016-10-25 2025-02-03 리제너론 파아마슈티컬스, 인크. 크로마토그래피 데이터 분석을 위한 방법 및 시스템
WO2019060062A1 (en) 2017-09-19 2019-03-28 Regeneron Pharmaceuticals Inc. METHODS OF REDUCING PARTICLE FORMATION AND COMPOSITIONS FORMED THEREFROM
TWI919355B (zh) 2018-07-02 2026-03-21 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
WO2020072062A1 (en) * 2018-10-04 2020-04-09 Gsw Creative Corporation Portable charging case
EA202192422A1 (ru) 2019-03-05 2021-12-09 Ридженерон Фармасьютикалз, Инк. Человеческий сывороточный альбумин в составах
CN110066789B (zh) * 2019-05-17 2021-04-20 通用生物系统（安徽）有限公司 一种长链dna引物的hplc纯化改进的方法
CN114025805B (zh) * 2019-06-07 2025-01-24 达因疗法公司 制备蛋白质-寡核苷酸复合物的方法
AU2021225957A1 (en) 2020-02-28 2022-09-08 Ionis Pharmaceuticals, Inc. Compounds and methods for modulating SMN2
WO2022187312A1 (en) * 2021-03-02 2022-09-09 Ionis Pharmaceuticals, Inc. Purification methods for oligomeric compounds
JP7897322B2 (ja) * 2022-09-07 2026-07-29 株式会社日本触媒 核酸の製造方法、不純物の除去方法及び不純物が少ない核酸
CN117050120A (zh) * 2022-12-09 2023-11-14 苏州赛分科技股份有限公司 一种纯化小干扰rna的柱层析法
WO2024175051A1 (zh) * 2023-02-23 2024-08-29 奥安医药（苏州）有限公司 一种疏水层析制备寡核苷酸的方法
WO2025149349A1 (en) 2024-01-09 2025-07-17 Cytiva Bioprocess R&D Ab Method for separating nucleic acid molecules
WO2025234897A1 (ru) * 2024-05-07 2025-11-13 Акционерное общество "ГЕНЕРИУМ" Способ получения тиофосфатных олигонуклеотидов для терапевтического применения

Citations (1)

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WO1996001268A1 (en) * 1994-07-05 1996-01-18 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography

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JPS60104097A (ja) * 1983-11-08 1985-06-08 Sagami Chem Res Center Ｄνａの精製方法
DE3433649A1 (de) * 1984-09-13 1986-03-20 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur aufreinigung synthetischer oligonucleotide
US6087491A (en) * 1993-01-08 2000-07-11 Hybridon, Inc. Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks
CN1155887A (zh) * 1994-07-05 1997-07-30 海布里顿公司 用deae-5pw阴离子-离子交换色谱和疏水作用色谱纯化硫代磷酸寡脱氧核苷酸
JP2001520660A (ja) * 1997-04-21 2001-10-30 プロリゴ・エルエルシー オリゴヌクレオチドの溶液相合成方法
JP2000035423A (ja) 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
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PT102491B (pt) * 2000-07-10 2003-02-28 Inst Superior Tecnico Processo para producao e purificacao de dna plasmidico
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PT2548560E (pt) 2005-06-23 2015-09-22 Isis Pharmaceuticals Inc Composições e métodos de modulação da excisão-união de smn2
DE602007009487D1 (de) 2006-01-27 2010-11-11 Isis Pharmaceutical Inc 6-modifizierte bicyclische nukleinsäureanaloga
SG2014015010A (en) * 2008-04-30 2014-05-29 Gradalis Inc Highly pure plasmid dna preparations and processes for preparing the same
KR20220084437A (ko) 2009-06-17 2022-06-21 바이오젠 엠에이 인코포레이티드 대상에게서 ｓｍｎ２ 스플라이싱을 조정하기 위한 조성물 및 방법
HUE050704T2 (hu) 2014-04-01 2020-12-28 Biogen Ma Inc Összetételek a SOD-1 expressziójának modulálására

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
WO1996001268A1 (en) * 1994-07-05 1996-01-18 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography

Also Published As

Publication number Publication date
AU2017284202A1 (en) 2019-01-17
US10947262B2 (en) 2021-03-16
JP2019518759A (ja) 2019-07-04
KR20190018467A (ko) 2019-02-22
KR102461234B1 (ko) 2022-10-28
BR112018075667B1 (pt) 2024-02-20
CN109641928B (zh) 2022-08-30
JP7075359B2 (ja) 2022-05-25
WO2017218454A1 (en) 2017-12-21
CN109641928A (zh) 2019-04-16
ES2988362T3 (es) 2024-11-20
US20190248823A1 (en) 2019-08-15
IL263639A (en) 2019-01-31
BR112018075667A2 (pt) 2019-04-09
MX2018015654A (es) 2019-06-10
US20210269467A1 (en) 2021-09-02
EP3468981B1 (en) 2024-07-24
EP3468981A1 (en) 2019-04-17
US20250011357A1 (en) 2025-01-09
IL263639B (en) 2021-09-30
NZ749360A (en) 2025-11-28
CA3027288A1 (en) 2017-12-21

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