JP2018508198A - 別個の実体におけるバーコード付加による核酸のシーケンシング - Google Patents

別個の実体におけるバーコード付加による核酸のシーケンシング Download PDF

Info

Publication number
JP2018508198A
JP2018508198A JP2017539228A JP2017539228A JP2018508198A JP 2018508198 A JP2018508198 A JP 2018508198A JP 2017539228 A JP2017539228 A JP 2017539228A JP 2017539228 A JP2017539228 A JP 2017539228A JP 2018508198 A JP2018508198 A JP 2018508198A
Authority
JP
Japan
Prior art keywords
nucleic acid
sequence
separate
acid barcode
entity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017539228A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018508198A5 (cg-RX-API-DMAC7.html
Inventor
アール. アベート,アダム
アール. アベート,アダム
ハリバートン,ジョン
ラン,フリーマン
アール. シャンビ,アダム
アール. シャンビ,アダム
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
University of California Berkeley
Original Assignee
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California San Diego UCSD filed Critical University of California San Diego UCSD
Publication of JP2018508198A publication Critical patent/JP2018508198A/ja
Publication of JP2018508198A5 publication Critical patent/JP2018508198A5/ja
Priority to JP2022023107A priority Critical patent/JP2022088360A/ja
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40Static mixers
    • B01F25/42Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/433Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
    • B01F25/4338Mixers with a succession of converging-diverging cross-sections, i.e. undulating cross-section
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30Micromixers
    • B01F33/302Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02Investigating particle size or size distribution
    • G01N15/0205Investigating particle size or size distribution by optical means
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404Handling flow, e.g. hydrodynamic focusing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484Optical investigation techniques, e.g. flow cytometry microstructural devices
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F21/00Dissolving
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652Sorting or classification of particles or molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/02Identification, exchange or storage of information
    • B01L2300/021Identification, e.g. bar codes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0627Sensor or part of a sensor is integrated
    • B01L2300/0645Electrodes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867Multiple inlets and one sample wells, e.g. mixing, dilution
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/12Specific details about materials
    • B01L2300/123Flexible; Elastomeric
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424Dielectrophoretic forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/149Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02Investigating particle size or size distribution
    • G01N2015/0288Sorting the particles
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02Investigating particle size or size distribution
    • G01N2015/0294Particle shape
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493Particle size
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497Particle shape

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
JP2017539228A 2015-02-04 2016-02-03 別個の実体におけるバーコード付加による核酸のシーケンシング Pending JP2018508198A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022023107A JP2022088360A (ja) 2015-02-04 2022-02-17 別個の実体におけるバーコード付加による核酸のシーケンシング

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562112075P 2015-02-04 2015-02-04
US62/112,075 2015-02-04
PCT/US2016/016444 WO2016126871A2 (en) 2015-02-04 2016-02-03 Sequencing of nucleic acids via barcoding in discrete entities

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022023107A Division JP2022088360A (ja) 2015-02-04 2022-02-17 別個の実体におけるバーコード付加による核酸のシーケンシング

Publications (2)

Publication Number Publication Date
JP2018508198A true JP2018508198A (ja) 2018-03-29
JP2018508198A5 JP2018508198A5 (cg-RX-API-DMAC7.html) 2019-03-14

Family

ID=56564869

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2017539228A Pending JP2018508198A (ja) 2015-02-04 2016-02-03 別個の実体におけるバーコード付加による核酸のシーケンシング
JP2022023107A Pending JP2022088360A (ja) 2015-02-04 2022-02-17 別個の実体におけるバーコード付加による核酸のシーケンシング

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2022023107A Pending JP2022088360A (ja) 2015-02-04 2022-02-17 別個の実体におけるバーコード付加による核酸のシーケンシング

Country Status (7)

Country Link
US (3) US11111519B2 (cg-RX-API-DMAC7.html)
EP (2) EP4112744B1 (cg-RX-API-DMAC7.html)
JP (2) JP2018508198A (cg-RX-API-DMAC7.html)
CN (2) CN115011670A (cg-RX-API-DMAC7.html)
AU (2) AU2016215304B2 (cg-RX-API-DMAC7.html)
CA (1) CA2974306A1 (cg-RX-API-DMAC7.html)
WO (1) WO2016126871A2 (cg-RX-API-DMAC7.html)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018014924A (ja) * 2016-07-27 2018-02-01 国立大学法人京都大学 ヘリカーゼを用いたpcr
US10745762B2 (en) 2012-08-13 2020-08-18 The Regents Of The University Of California Method and system for synthesizing a target polynucleotide within a droplet
US11020736B2 (en) 2014-10-22 2021-06-01 The Regents Of The University Of California High definition microdroplet printer
US11111519B2 (en) 2015-02-04 2021-09-07 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US11124830B2 (en) 2016-12-21 2021-09-21 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
WO2021241721A1 (ja) * 2020-05-29 2021-12-02 国立研究開発法人理化学研究所 細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法
JP2021534956A (ja) * 2018-08-17 2021-12-16 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California 安定なジェットからの単分散粒子に誘発される液滴の形成
JP2022522221A (ja) * 2019-04-02 2022-04-14 ミッション バイオ インコーポレイテッド 腫瘍を特性決定し、腫瘍の不均質性を識別するための方法及びシステム
US11312990B2 (en) 2014-06-27 2022-04-26 The Regents Of The University Of California PCR-activated sorting (PAS)
US11365441B2 (en) 2019-05-22 2022-06-21 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
US11781129B2 (en) 2017-10-18 2023-10-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis

Families Citing this family (218)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
US10564147B2 (en) 2012-05-25 2020-02-18 The Regents Of The University Of California Microfluidic systems for particle trapping and separation using cavity acoustic transducers
BR112015003354A8 (pt) 2012-08-14 2018-01-16 10X Genomics Inc métodos e composições de microcápsula
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2900481A1 (en) 2013-02-08 2014-08-14 10X Genomics, Inc. Polynucleotide barcode generation
EP3597772A1 (en) 2013-04-17 2020-01-22 Agency For Science, Technology And Research Method for generating extended sequence reads
LT3013983T (lt) 2013-06-25 2023-05-10 Prognosys Biosciences, Inc. Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį
EP3039158B1 (en) 2013-08-28 2018-11-14 Cellular Research, Inc. Massively parallel single cell analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
KR102596508B1 (ko) 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
GB201414177D0 (en) * 2014-08-11 2014-09-24 Sphere Fluidics Ltd Droplet Sorting
US20160122817A1 (en) 2014-10-29 2016-05-05 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
CN107532202A (zh) 2015-02-24 2018-01-02 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
CN107849560B (zh) * 2015-04-27 2021-10-22 阿布维特罗有限责任公司 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法
GB201509640D0 (en) * 2015-06-03 2015-07-15 Sphere Fluidics Ltd Systems and methods
JP2018525004A (ja) * 2015-08-17 2018-09-06 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 微小液滴ベースの多置換増幅(mda)方法及び関連組成物
US9862941B2 (en) 2015-10-14 2018-01-09 Pioneer Hi-Bred International, Inc. Single cell microfluidic device
EP3374083B1 (en) * 2015-11-10 2021-01-06 Illumina, Inc. Inertial droplet generation and particle encapsulation
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
EP4144861B1 (en) 2015-12-04 2024-09-11 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
JP6735348B2 (ja) 2016-02-11 2020-08-05 10エックス ジェノミクス, インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2017181161A1 (en) 2016-04-15 2017-10-19 Predicine, Inc. Systems and methods for detecting genetic alterations
WO2018057820A1 (en) 2016-09-21 2018-03-29 Predicine, Inc. Systems and methods for combined detection of genetic alterations
WO2018183796A1 (en) * 2017-03-31 2018-10-04 Predicine, Inc. Systems and methods for predicting and monitoring cancer therapy
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US11513076B2 (en) 2016-06-15 2022-11-29 Ludwig-Maximilians-Universität München Single molecule detection or quantification using DNA nanotechnology
EP3485215B1 (en) 2016-07-12 2023-06-07 Alexander Poltorak System and method for maintaining efficiency of a heat sink
CA3032528A1 (en) 2016-09-15 2018-03-22 Augmenta Bioworks, Inc. Immune repertoire sequence amplification methods and applications
EP3516400B1 (en) 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018067792A1 (en) * 2016-10-07 2018-04-12 President And Fellows Of Harvard College Sequencing of bacteria or other species
US11090653B2 (en) 2016-10-11 2021-08-17 The Regents Of The University Of California Systems and methods to encapsulate and preserve organic matter for analysis
WO2018089550A1 (en) 2016-11-10 2018-05-17 Takara Bio Usa, Inc. Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
JP7179008B2 (ja) * 2016-11-16 2022-11-28 カタログ テクノロジーズ, インコーポレイテッド 核酸ベースのデータ記憶
US20190316180A1 (en) * 2016-11-23 2019-10-17 Universite De Strasbourg Tandem barcoding of target molecules for their absolute quantification at single-entity resolution
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) * 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP4218738B1 (en) * 2017-02-24 2024-10-16 The Regents of The University of California Particle-drop structures and methods for making and using the same
CN107083423B (zh) * 2017-03-27 2022-01-28 北京极客基因科技有限公司 一种药物靶点预测和药物全方面评价方法
US12054764B2 (en) 2017-04-14 2024-08-06 The Broad Institute, Inc. High-throughput screens for exploring biological functions of microscale biological systems
CN110945139B (zh) 2017-05-18 2023-09-05 10X基因组学有限公司 用于分选液滴和珠的方法和系统
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
MA49352A (fr) * 2017-05-26 2020-04-08 Abvitro Llc Séquençage de bibliothèque de polynucléotides à haut rendement et analyse de transcriptome
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
WO2018227210A1 (en) 2017-06-09 2018-12-13 The Regents Of The University Of California High-efficiency encapsulation in droplets based on hydrodynamic vortices control
US11517901B2 (en) 2017-06-09 2022-12-06 The Regents Of The University Of California High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting
EP3642363B1 (en) * 2017-06-20 2025-12-24 Bio-Rad Laboratories, Inc. Mda using bead oligonucleotide
TWI637834B (zh) * 2017-07-11 2018-10-11 台灣奈米碳素股份有限公司 微流道裝置的製造方法及其結構
US11031312B2 (en) 2017-07-17 2021-06-08 Fractal Heatsink Technologies, LLC Multi-fractal heatsink system and method
WO2019033062A2 (en) * 2017-08-10 2019-02-14 Metabiotech Corporation MARKING OF NUCLEIC ACID MOLECULES OF INDIVIDUAL CELLS FOR STEP SEQUENCING
US10357771B2 (en) 2017-08-22 2019-07-23 10X Genomics, Inc. Method of producing emulsions
US11680283B2 (en) 2017-09-22 2023-06-20 University Of Washington In situ combinatorial labeling of cellular molecules
US11639933B2 (en) * 2017-09-27 2023-05-02 Bio-Rad Laboratories, Inc. Digital affinity linkage assay
EP4494757A3 (en) 2017-09-29 2025-03-19 The Regents of The University of California Method of generating monodisperse emulsions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019075409A1 (en) 2017-10-12 2019-04-18 The Regents Of The University Of California ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM)
US20190112655A1 (en) * 2017-10-18 2019-04-18 Mission Bio, Inc. Method, Systems and Apparatus for High-Throughput Single-Cell DNA Sequencing With Droplet Microfluidics
US11745179B2 (en) 2017-10-20 2023-09-05 The Regents Of The University Of California Microfluidic systems and methods for lipoplex-mediated cell transfection
US11499127B2 (en) 2017-10-20 2022-11-15 The Regents Of The University Of California Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks
WO2019084055A1 (en) * 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN114525273B (zh) 2017-10-27 2025-01-28 10X基因组学有限公司 用于样品制备和分析的方法和系统
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019104337A1 (en) 2017-11-27 2019-05-31 The Trustees Of Columbia University In The City Of New York Rna printing and sequencing devices, methods, and systems
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019113235A1 (en) * 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
WO2019118355A1 (en) * 2017-12-12 2019-06-20 10X Genomics, Inc. Systems and methods for single cell processing
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
US20200392562A1 (en) * 2017-12-22 2020-12-17 Thermo Fisher Scientific Baltics Uab Polymerase chain reaction composition comprising amines
CN112005115A (zh) 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) * 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
CN121006394A (zh) * 2018-03-02 2025-11-25 量子细胞公司 从组织中分离和表达分析感兴趣的区域的方法、组成和装置
US11827922B2 (en) 2018-03-07 2023-11-28 Wisconsin Alumni Research Foundation High throughput nucleic acid profiling of single cells
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
KR20240052875A (ko) 2018-04-20 2024-04-23 일루미나, 인코포레이티드 단일 세포를 캡슐화하는 방법, 캡슐화된 세포 및 이의 용도
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US20210214791A1 (en) * 2018-05-22 2021-07-15 Universite De Strasbourg Absolute quantification of target molecules at single-entity resolution using tandem barcoding
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
WO2020009998A1 (en) * 2018-07-03 2020-01-09 Chan Zuckerberg Biohub, Inc. Method to perform high-throughput single cell genomic and phenotypic analyses
EP3818166A4 (en) * 2018-07-05 2022-03-30 AccuraGen Holdings Limited Compositions and methods for digital polymerase chain reaction
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3830289A1 (en) 2018-08-03 2021-06-09 10X Genomics, Inc. Methods and systems to minimize barcode exchange
JP7576536B2 (ja) 2018-08-03 2024-10-31 カタログ テクノロジーズ, インコーポレイテッド 誤り保護とともに核酸に基づくデータを記憶および読むためのシステムおよび方法
WO2020033332A1 (en) * 2018-08-07 2020-02-13 Augmenta Bioworks, Inc. Methods for making gel beads and core and shell beads with a cell
US20210301247A1 (en) * 2018-08-10 2021-09-30 Cellino Biotech, Inc. System for image-driven cell manufacturing
US12065688B2 (en) * 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
WO2020077017A2 (en) 2018-10-10 2020-04-16 Augmenta Bioworks, Inc. Methods for isolating immune binding proteins
US11608524B1 (en) * 2018-10-25 2023-03-21 Wisconsin Alumni Research Foundation Methods of analyzing cells
EP4656734A2 (en) * 2018-10-26 2025-12-03 Illumina, Inc. Modulating polymer beads for dna processing
EP4567127A3 (en) * 2018-12-10 2025-09-03 10x Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
KR20210104779A (ko) 2018-12-13 2021-08-25 디엔에이 스크립트 세포 및 생체분자 상의 직접 올리고뉴클레오타이드 합성
CN111378557B (zh) 2018-12-26 2023-06-06 财团法人工业技术研究院 用于产生液珠的管状结构及液珠产生方法
EP3902922A1 (en) * 2018-12-28 2021-11-03 Biobloxx AB Method and kit for preparing complementary dna
CN113874521B (zh) * 2019-01-06 2024-11-01 10X基因组学有限公司 用于富集条形码的方法和系统
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US20200232011A1 (en) * 2019-01-22 2020-07-23 Mission Bio Methods of nucleic acid detection and primer design
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
WO2020183591A1 (en) * 2019-03-11 2020-09-17 Enplas Corporation Fluid handling method, and fluid handling device and fluid handling system used for the method
US20210277458A1 (en) * 2019-04-02 2021-09-09 Mission Bio, Inc. Methods, systems, and aparatus for nucleic acid detection
WO2020252387A2 (en) * 2019-06-12 2020-12-17 Ultima Genomics, Inc. Methods for accurate base calling using molecular barcodes
WO2020264387A1 (en) * 2019-06-27 2020-12-30 Cell Microsystems, Inc. Systems and methods for associating single cell imaging with rna transcriptomics
US12186751B2 (en) 2019-06-28 2025-01-07 10X Genomics, Inc. Devices and systems incorporating acoustic ordering and methods of use thereof
WO2021007724A1 (zh) * 2019-07-12 2021-01-21 深圳华大生命科学研究院 一种全基因组全流程微流控自动化建库方法和装置
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
EP4004201A4 (en) * 2019-07-31 2023-08-23 Bioskryb Genomics, Inc. Single cell analysis
AU2020325026A1 (en) 2019-08-05 2022-03-17 Mission Bio, Inc. Method and apparatus for single-cell analysis for determining a cell trajectory
US12179199B2 (en) 2019-08-09 2024-12-31 The Regents Of The University Of California Microfluidic single-cell pairing array for studying cell-cell interactions in isolated compartments
CA3150030A1 (en) * 2019-09-04 2021-03-11 Marion Francis SETLIFF Methods for identification of antigen binding specificity of antibodies
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
WO2021067286A1 (en) * 2019-09-30 2021-04-08 Shield Diagnostics Corp. Devices for determining a target nucleic acid profile and methods of use thereof
AU2020357191A1 (en) * 2019-10-05 2022-06-02 Mission Bio, Inc. Methods, systems and apparatus for copy number variations and single nucleotide variations simultaneously detected in single-cells
GB2604481A (en) 2019-10-10 2022-09-07 1859 Inc Methods and systems for microfluidic screening
WO2021076841A1 (en) * 2019-10-16 2021-04-22 Georgia Tech Research Corporation Methods for ultrasensitive detection of protein and cellular biomarkers
CN110777048A (zh) * 2019-11-01 2020-02-11 上海市第六人民医院 一种高通量单外泌体分离装置
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
EP4058596A1 (en) * 2019-11-15 2022-09-21 Miltenyi Biotec B.V. & Co. KG Color and bardcoded beads for single cell indexing
US12059679B2 (en) * 2019-11-19 2024-08-13 10X Genomics, Inc. Methods and devices for sorting droplets and particles
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
GB201918340D0 (en) * 2019-12-12 2020-01-29 Cambridge Entpr Ltd Spatial barcoding
US12241890B2 (en) 2019-12-23 2025-03-04 10X Genomics, Inc. Methods for generating barcoded nucleic acid molecules using fixed cells
WO2021128035A1 (zh) * 2019-12-25 2021-07-01 苏州绘真生物科技有限公司 高通量单细胞转录组测序方法及试剂盒
CA3167719A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Methods and systems for single cell gene profiling
CA3167729A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Emulsion based drug screening
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
JP2023511279A (ja) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞シーケンシング
US20230045126A1 (en) * 2020-01-14 2023-02-09 President And Fellows Of Harvard College Devices and methods for determining nucleic acids using digital droplet pcr and related techniques
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
EP4095256A4 (en) * 2020-01-23 2023-03-15 DGI Tech (Qing Dao) Co., Limited Droplet microfluidics-based single cell sequencing and applications
EP4093884A4 (en) 2020-01-24 2024-05-15 Vanderbilt University METHODS FOR IDENTIFYING LIGAND-BLOCKING ANTIBODIES AND DETERMINING ANTIBODY POWER
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
CN111454942A (zh) * 2020-03-16 2020-07-28 张晓鲁 一种同一样品的转录组和基因组同时建立测序文库的构建方法
WO2021188500A1 (en) 2020-03-16 2021-09-23 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
CN115768884A (zh) * 2020-03-20 2023-03-07 使命生物公司 用于全基因组扩增的单细胞工作流程
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
CN111394432B (zh) * 2020-03-27 2023-03-24 深圳闪量科技有限公司 基于通用探针芯片的多重定量pcr检测系统
CN115997030A (zh) 2020-04-30 2023-04-21 德迈逊科技有限公司 用于大分子操作的装置和方法
GB202007059D0 (en) * 2020-05-13 2020-06-24 Univ Oxford Innovation Ltd Polynucleotide arrays
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022015970A1 (en) 2020-07-15 2022-01-20 Fluent Biosciences Inc. Tiered ligation oligos
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN114073995A (zh) * 2020-08-13 2022-02-22 安行生物技术有限公司 分析单细胞的微流控设备和单元及其方法
GB202012730D0 (en) * 2020-08-14 2020-09-30 Cambridge Entpr Ltd Modular microfluidic devices, systems and methodds for total RNA analyses
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US20230390770A1 (en) * 2020-11-09 2023-12-07 The General Hospital Corporation High Throughput Microfluidics for Analysis of Immune Cell Activation
EP4244379A1 (en) * 2020-11-13 2023-09-20 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US20220243264A1 (en) * 2020-11-23 2022-08-04 The Broad Institute, Inc. Systems and methods for amplifying rna
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
CN113322254B (zh) * 2021-01-06 2022-05-20 南京诺唯赞生物科技股份有限公司 多靶点蛋白质-dna相互作用的研究方法和工具
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
CN114836838A (zh) * 2021-02-01 2022-08-02 南方医科大学 一种中通量单细胞拷贝数文库构建的方法及其应用
CN120158497A (zh) 2021-02-23 2025-06-17 10X基因组学有限公司 基于探针的核酸和蛋白质分析
US20240209350A1 (en) * 2021-04-26 2024-06-27 European Molecular Biology Laboratory High complexity microcompartment-based interaction screening
CN117957330A (zh) * 2021-09-14 2024-04-30 贝克顿迪金森公司 全长单细胞rna测序
WO2023154816A1 (en) * 2022-02-09 2023-08-17 Mission Bio, Inc. Systems and methods of detecting merged droplets in single cell sequencing
DK181862B1 (en) * 2022-03-21 2025-02-24 Samplix Aps Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning
US20250297243A1 (en) * 2022-04-28 2025-09-25 BioSkryb Genomics, Inc. Single cell multiomics
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
EP4612283A1 (en) * 2022-11-04 2025-09-10 Fluid Discovery Systems, compositions, and methods for single cell analysis
KR20250137709A (ko) * 2023-01-30 2025-09-18 아이덱스 래보러토리즈, 인코포레이티드 전기습윤 바코드 자성 비드를 위한 방법, 시스템 및 디바이스
WO2025040497A1 (en) * 2023-08-18 2025-02-27 Qiagen Gmbh Method for improving nucleic acid processing
CN120783873B (zh) * 2025-07-09 2025-12-05 上海锦测医学检验所有限公司 一种智能化蛋白质组学数据异常检测与分析方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012109600A2 (en) * 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US20120220494A1 (en) * 2011-02-18 2012-08-30 Raindance Technolgies, Inc. Compositions and methods for molecular labeling
US20140272988A1 (en) * 2013-03-14 2014-09-18 Cold Spring Harbor Laboratory Trans-splicing transcriptome profiling
US20140323316A1 (en) * 2013-03-15 2014-10-30 Complete Genomics, Inc. Multiple tagging of individual long dna fragments
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing

Family Cites Families (118)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2150477A1 (en) 1992-11-30 1994-06-09 Robert Allen Snow Sequential targeting of tumor sites with oligonucleotide conjugates of antibody and complementary oligonucleotide conjugates of chelated radionuclide
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6319507B1 (en) 1997-05-02 2001-11-20 Kobo Products, Inc. Agar gel bead composition and method
EP1908832B1 (en) 1997-07-07 2012-12-26 Medical Research Council A method for increasing the concentration of a nucleic acid molecule
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
AU2002353107A1 (en) 2001-12-11 2003-07-09 Sau Lan Tang Staats Microfluidic devices and methods for two-dimensional separations
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
DE10339452A1 (de) 2003-08-22 2005-03-17 Institut für Physikalische Hochtechnologie e.V. Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten
JP4630870B2 (ja) 2003-08-27 2011-02-09 プレジデント アンド フェロウズ オブ ハーバード カレッジ 流体種の電子的制御
US20050069939A1 (en) 2003-09-26 2005-03-31 Youxiang Wang Amplification of polynucleotides by rolling circle amplification
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
KR100647282B1 (ko) 2003-12-24 2006-11-17 삼성전자주식회사 DNA 마이크로어레이 스폿팅(Spoting) 장치 및이를 이용한 스폿팅 방법
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060201868A1 (en) 2005-03-11 2006-09-14 Simmons Blake A Methods and devices for high-throughput dielectrophoretic concentration
US9285297B2 (en) 2005-08-22 2016-03-15 Applied Biosystems, Llc Device, system, and method for depositing processed immiscible-fluid-discrete-volumes
US8383059B2 (en) 2005-09-30 2013-02-26 University Of Utah Research Foundation Microfluidic interface for highly parallel addressing of sensing arrays
AU2007249635B2 (en) 2005-10-07 2012-05-31 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
US9183349B2 (en) 2005-12-16 2015-11-10 Nextbio Sequence-centric scientific information management
CA2636855C (en) 2006-01-11 2016-09-27 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
CA2653321A1 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc. Biochemical analysis of partitioned cells
PL2027470T3 (pl) 2006-06-02 2013-03-29 Pfizer Prod Inc Oznaczenie krążących komórek nowotworowych
EP2412812B1 (en) 2006-12-14 2015-02-11 Dow AgroSciences LLC Optimized non-canonical zinc finger proteins
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US8454906B2 (en) 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
GB0720202D0 (en) * 2007-10-16 2007-11-28 Cambridge Entpr Ltd Microfluidic systems
WO2009092106A1 (en) 2008-01-18 2009-07-23 The Regents Of The University Of California Accurate and rapid micromixer for integrated microfluidic devices
AU2009220203B2 (en) 2008-03-04 2014-02-20 Crystal Bioscience Inc. Gel microdrop composition and method of using the same
US9409177B2 (en) 2008-03-21 2016-08-09 Lawrence Livermore National Security, Llc Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
WO2011120024A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet generation for droplet-based assays
WO2013016459A1 (en) 2011-07-25 2013-01-31 Bio-Rad Laboratories, Inc. Breakage of an emulsion containing nucleic acid
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US20130084572A1 (en) 2011-09-30 2013-04-04 Quantalife, Inc. Calibrations and controls for droplet-based assays
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
EP2443236B1 (en) 2009-06-15 2015-05-13 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
SG177369A1 (en) 2009-06-26 2012-02-28 Harvard College Fluid injection
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
US8746285B2 (en) 2009-09-04 2014-06-10 Auburn University Programmable fluidic droplet generation
US9376713B2 (en) 2009-09-23 2016-06-28 The Board Of Trustees Of The University Of Illinois Label free detection of nucleic acid amplification
EP2957641B1 (en) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US20120270739A1 (en) 2010-01-19 2012-10-25 Verinata Health, Inc. Method for sample analysis of aneuploidies in maternal samples
AU2013203624B2 (en) 2010-02-09 2015-06-25 General Electric Company Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP2556170A4 (en) 2010-03-25 2014-01-01 Quantalife Inc TRAPPING TRANSPORT AND DETECTION SYSTEM
JP5573335B2 (ja) 2010-04-28 2014-08-20 株式会社島津製作所 磁性体粒子操作デバイス及び磁性体粒子操作方法
DK2577254T3 (en) 2010-06-10 2015-06-01 Univ Freiburg Albert Ludwigs An apparatus and method for delivering cells or particles that are encased in a freely suspended droplet
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
JP5893620B2 (ja) 2010-07-22 2016-03-23 ジェンセル バイオシステムズ リミテッド 合成液体セル
EP2608878A4 (en) 2010-08-23 2017-11-15 President and Fellows of Harvard College Acoustic waves in microfluidics
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
EP2436444A1 (en) 2010-10-01 2012-04-04 Centre National de la Recherche Scientifique (C.N.R.S.) Microfluidic device for production and collection of droplets of a fluid
ES2966040T3 (es) 2010-10-08 2024-04-18 Harvard College Alto rendimiento de células individuales con código de barra
US20120258881A1 (en) 2010-11-22 2012-10-11 The University Of Chicago Methods and/or Use of Oligonucleotide Conjugates for Assays and Microscopy/Imaging Detections
DK2652155T3 (en) 2010-12-16 2017-02-13 Gigagen Inc Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells
US20130295587A1 (en) 2011-01-24 2013-11-07 Ge Healthcare Bio-Sciences Ab Method of coupling binding agents to a substrate surface
US8765455B2 (en) 2011-01-27 2014-07-01 Lawrence Livermore National Security, Llc Chip-based droplet sorting
ES2687761T3 (es) * 2011-01-31 2018-10-29 F. Hoffmann-La Roche Ag Métodos de identificación de múltiples epítopos en células
CA2826131C (en) 2011-02-02 2019-11-05 Jay Ashok Shendure Massively parallel continguity mapping
PL3246416T3 (pl) 2011-04-15 2024-09-30 The Johns Hopkins University Bezpieczny system sekwencjonowania
AU2012249759A1 (en) 2011-04-25 2013-11-07 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US9074204B2 (en) 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
DE202012013668U1 (de) * 2011-06-02 2019-04-18 Raindance Technologies, Inc. Enzymquantifizierung
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
KR101974492B1 (ko) 2011-07-26 2019-05-02 베리나타 헬스, 인코포레이티드 샘플 중 상이한 이수성의 존재 또는 부재를 결정하는 방법
US20130032235A1 (en) 2011-08-02 2013-02-07 Teledyne Dalsa Semiconductor, Inc. Integrated microfluidic check valve and device including such a check valve
US9469874B2 (en) * 2011-10-18 2016-10-18 The Regents Of The University Of California Long-range barcode labeling-sequencing
WO2013095469A1 (en) 2011-12-21 2013-06-27 Intel Corporation Psmi using at-speed scan capture
JP2015513451A (ja) 2012-02-08 2015-05-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 流体分割を用いる液滴形成
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
WO2013134261A1 (en) 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
EP2850211B1 (en) 2012-05-14 2021-09-08 iRepertoire, Inc. Method for increasing accuracy in quantitative detection of polynucleotides
US10161007B2 (en) 2012-08-13 2018-12-25 The Regents Of The University Of California Methods and systems for detecting biological components
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
BR112015003354A8 (pt) 2012-08-14 2018-01-16 10X Genomics Inc métodos e composições de microcápsula
WO2014039912A1 (en) 2012-09-07 2014-03-13 Bio-Rad Laboratories, Inc. Compositions, systems and methods for droplet formation, spacing and detection
EP2898071A4 (en) 2012-09-21 2016-07-20 Broad Inst Inc COMPOSITIONS AND METHODS FOR LONG-TERM LABORATORIES AND PREPARED END OF NUCLEIC ACIDS IN EMULSION DROPS
KR20150090037A (ko) 2012-11-27 2015-08-05 젠셀 바이오시스템즈 리미티드 액체 샘플의 취급
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014108323A1 (en) 2013-01-10 2014-07-17 Eth Zurich Method and apparatus for depositing droplets onto a substrate
CN105324489A (zh) 2013-03-04 2016-02-10 韦拉克斯生物医学股份有限公司 多分析物试验
WO2014153071A1 (en) 2013-03-14 2014-09-25 The Broad Institute, Inc. Methods for quantitating dna using digital multiple displacement amplification
WO2014144495A1 (en) * 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
WO2014151658A1 (en) 2013-03-15 2014-09-25 The Regents Of The University Of California High-speed on demand microfluidic droplet generation and manipulation
DE102013207900A1 (de) 2013-04-30 2014-10-30 Volkswagen Ag Membran-Elektroden-Einheit und Brennstoffzelle mit einer solchen
EP3039158B1 (en) 2013-08-28 2018-11-14 Cellular Research, Inc. Massively parallel single cell analysis
WO2015069798A1 (en) 2013-11-05 2015-05-14 The Regents Of The University Of California Single-cell forensic short tandem repeat typing within microfluidic droplets
WO2015120398A1 (en) 2014-02-10 2015-08-13 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
CN113215221A (zh) 2014-04-08 2021-08-06 华盛顿大学商业中心 用于使用多分散小滴执行数字检定的方法和设备
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN107075543B (zh) 2014-04-21 2021-11-16 哈佛学院院长及董事 用于条形码化核酸的系统和方法
CA2949537C (en) 2014-05-23 2023-02-14 Firefly Bioworks, Inc. Substrate-mediated reactors for bioassays
JP6666268B2 (ja) 2014-06-11 2020-03-13 サンプリクス アンパーツゼルスカブ 液滴ソートによるヌクレオチド配列排除富化(needls)
EP4574974A3 (en) * 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
WO2015200717A2 (en) 2014-06-27 2015-12-30 The Regents Of The University Of California Pcr-activated sorting (pas)
WO2016064755A2 (en) 2014-10-20 2016-04-28 The Regents Of The University Of Califronia Rapid modulation of droplet composition with membrane microvalves
WO2016065056A1 (en) 2014-10-22 2016-04-28 The Regents Of The University Of California High definition microdroplet printer
CA2974299A1 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Multiple-emulsion nucleic acid amplification
CA2974306A1 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
JP2018525004A (ja) 2015-08-17 2018-09-06 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 微小液滴ベースの多置換増幅(mda)方法及び関連組成物
US20190127789A1 (en) 2016-04-19 2019-05-02 President And Fellows Of Harvard College Immobilization-based systems and methods for genetic analysis and other applications
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
AU2017382905A1 (en) 2016-12-21 2019-07-04 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
WO2019099908A1 (en) 2017-11-17 2019-05-23 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012109600A2 (en) * 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US20120220494A1 (en) * 2011-02-18 2012-08-30 Raindance Technolgies, Inc. Compositions and methods for molecular labeling
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140272988A1 (en) * 2013-03-14 2014-09-18 Cold Spring Harbor Laboratory Trans-splicing transcriptome profiling
US20140323316A1 (en) * 2013-03-15 2014-10-30 Complete Genomics, Inc. Multiple tagging of individual long dna fragments

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11203787B2 (en) 2012-08-13 2021-12-21 The Regents Of The University Of California Methods and systems for detecting biological components
US10745762B2 (en) 2012-08-13 2020-08-18 The Regents Of The University Of California Method and system for synthesizing a target polynucleotide within a droplet
US11001896B2 (en) 2012-08-13 2021-05-11 The Regents Of The University Of California System and method to synthesize a target molecule within a droplet
US11891666B2 (en) 2012-08-13 2024-02-06 The Regents Of The University Of California Methods and systems for detecting biological components
US11312990B2 (en) 2014-06-27 2022-04-26 The Regents Of The University Of California PCR-activated sorting (PAS)
US11020736B2 (en) 2014-10-22 2021-06-01 The Regents Of The University Of California High definition microdroplet printer
US11732287B2 (en) 2015-02-04 2023-08-22 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US12448643B2 (en) 2015-02-04 2025-10-21 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US11111519B2 (en) 2015-02-04 2021-09-07 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
JP2018014924A (ja) * 2016-07-27 2018-02-01 国立大学法人京都大学 ヘリカーゼを用いたpcr
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
US11124830B2 (en) 2016-12-21 2021-09-21 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US11781129B2 (en) 2017-10-18 2023-10-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
JP2021534956A (ja) * 2018-08-17 2021-12-16 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California 安定なジェットからの単分散粒子に誘発される液滴の形成
JP7504078B2 (ja) 2018-08-17 2024-06-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 安定なジェットからの単分散粒子に誘発される液滴の形成
JP2022522221A (ja) * 2019-04-02 2022-04-14 ミッション バイオ インコーポレイテッド 腫瘍を特性決定し、腫瘍の不均質性を識別するための方法及びシステム
US11365441B2 (en) 2019-05-22 2022-06-21 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
WO2021241721A1 (ja) * 2020-05-29 2021-12-02 国立研究開発法人理化学研究所 細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法
JP7423101B2 (ja) 2020-05-29 2024-01-29 国立研究開発法人理化学研究所 細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法
JPWO2021241721A1 (cg-RX-API-DMAC7.html) * 2020-05-29 2021-12-02

Also Published As

Publication number Publication date
JP2022088360A (ja) 2022-06-14
US12448643B2 (en) 2025-10-21
AU2016215304A1 (en) 2017-08-10
EP3253479A4 (en) 2018-10-10
US11732287B2 (en) 2023-08-22
CA2974306A1 (en) 2016-08-11
US20240150811A1 (en) 2024-05-09
HK1248169A1 (en) 2018-10-12
WO2016126871A3 (en) 2016-10-06
EP4112744A1 (en) 2023-01-04
EP4112744B1 (en) 2025-08-27
EP3253479A2 (en) 2017-12-13
AU2022202780A1 (en) 2022-05-19
US11111519B2 (en) 2021-09-07
CN107530654A (zh) 2018-01-02
CN115011670A (zh) 2022-09-06
WO2016126871A2 (en) 2016-08-11
US20180216160A1 (en) 2018-08-02
AU2016215304B2 (en) 2022-01-27
EP3253479B1 (en) 2022-09-21
US20170009274A1 (en) 2017-01-12

Similar Documents

Publication Publication Date Title
US12448643B2 (en) Sequencing of nucleic acids via barcoding in discrete entities
US11312990B2 (en) PCR-activated sorting (PAS)
US20220154248A1 (en) Combined multiple-displacement amplification and pcr in an emulsion microdroplet
AU2022203056A1 (en) Multiple-emulsion nucleic acid amplification
Zhang et al. Single-molecule DNA amplification and analysis using microfluidics
JP2020535951A (ja) 単分散エマルションを生成する方法
JP2021534956A (ja) 安定なジェットからの単分散粒子に誘発される液滴の形成
Liu et al. Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics
HK1248169B (en) Sequencing of nucleic acids via barcoding in discrete entities
HK1248284B (en) Multiple-emulsion nucleic acid amplification

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190129

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190129

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191217

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200311

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200617

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20201117

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210216

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210413

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210514

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20211019

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220217

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20220217

C11 Written invitation by the commissioner to file amendments

Free format text: JAPANESE INTERMEDIATE CODE: C11

Effective date: 20220301

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220524

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20220613

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20220614

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20220902

C211 Notice of termination of reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C211

Effective date: 20220906