CN113891961A - 一种全基因组全流程微流控自动化建库方法和装置 - Google Patents

一种全基因组全流程微流控自动化建库方法和装置 Download PDF

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Publication number
CN113891961A
CN113891961A CN201980096864.9A CN201980096864A CN113891961A CN 113891961 A CN113891961 A CN 113891961A CN 201980096864 A CN201980096864 A CN 201980096864A CN 113891961 A CN113891961 A CN 113891961A
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dna
microliters
tween
genome
reaction
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崔淼
任悍
汪元涛
陈杨帆
江媛
陈奥
章文蔚
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BGI Shenzhen Co Ltd
Shenzhen BGI Life Science Research Institute
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BGI Shenzhen Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Structural Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

一种全基因组全流程微流控自动化建库方法和装置，其中建库方法包括：在数字化微流控芯片上，以基因组DNA作为建库起始原料，通过电润湿原理控制液滴在数字化微流控芯片上的移动，依次进行基因组DNA打断、末端修复、接头连接、单链环化和DNA纳米球制备的反应，其中每一步反应的反应体系不超过10微升，反应体系包含反应组分和用于降低表面张力的表面活性剂成分。利用数字化微流控实现快速自动化建库以服务个性化测序和精准医疗体系。本发明通过电润湿原理控制液滴移动，实现全基因组全流程微流控自动化建库，成本低，无污染。

Description

PCT国内申请，说明书已公开。

Claims (18)

  1. PCT国内申请，权利要求书已公开。
CN201980096864.9A 2019-07-12 2019-07-12 一种全基因组全流程微流控自动化建库方法和装置 Pending CN113891961A (zh)

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PCT/CN2019/095868 WO2021007724A1 (zh) 2019-07-12 2019-07-12 一种全基因组全流程微流控自动化建库方法和装置

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116367921A (zh) * 2021-10-27 2023-06-30 京东方科技集团股份有限公司 数字微流控芯片及其驱动方法、数字微流控装置
CN116814864B (zh) * 2023-08-30 2023-11-24 深圳赛陆医疗科技有限公司 基因测序中芯片转移控制方法及装置、设备、系统及介质

Citations (8)

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US20130165346A1 (en) * 2011-12-07 2013-06-27 The Johns Hopkins University System and method for screening a library of samples
US20130244906A1 (en) * 2011-05-05 2013-09-19 Biopico Systems Microfluidic devices and methods based on massively parallel picoreactors for cell and molecular diagnostics
WO2016045105A1 (zh) * 2014-09-26 2016-03-31 深圳华大基因股份有限公司 Pf快速建库方法及其应用
CN105734048A (zh) * 2016-02-26 2016-07-06 武汉冰港生物科技有限公司 一种基因组DNA的PCR-free测序文库制备方法
US20160298173A1 (en) * 2015-04-13 2016-10-13 The Johns Hopkins University Multiplexed, continuous-flow, droplet-based platform for high-throughput genetic detection
CN107530654A (zh) * 2015-02-04 2018-01-02 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
WO2018200872A1 (en) * 2017-04-26 2018-11-01 Berkeley Lights, Inc. Biological process systems and methods using microfluidic apparatus having an optimized electrowetting surface
CN109207471A (zh) * 2017-06-30 2019-01-15 深圳华大基因股份有限公司 一种构建分片段核酸文库的方法及其应用

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130244906A1 (en) * 2011-05-05 2013-09-19 Biopico Systems Microfluidic devices and methods based on massively parallel picoreactors for cell and molecular diagnostics
US20130165346A1 (en) * 2011-12-07 2013-06-27 The Johns Hopkins University System and method for screening a library of samples
WO2016045105A1 (zh) * 2014-09-26 2016-03-31 深圳华大基因股份有限公司 Pf快速建库方法及其应用
CN107530654A (zh) * 2015-02-04 2018-01-02 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
US20160298173A1 (en) * 2015-04-13 2016-10-13 The Johns Hopkins University Multiplexed, continuous-flow, droplet-based platform for high-throughput genetic detection
CN105734048A (zh) * 2016-02-26 2016-07-06 武汉冰港生物科技有限公司 一种基因组DNA的PCR-free测序文库制备方法
WO2018200872A1 (en) * 2017-04-26 2018-11-01 Berkeley Lights, Inc. Biological process systems and methods using microfluidic apparatus having an optimized electrowetting surface
CN109207471A (zh) * 2017-06-30 2019-01-15 深圳华大基因股份有限公司 一种构建分片段核酸文库的方法及其应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
牛嘉琦;王伟强;: "一种基于数字微流控技术的PCR微芯片", 国外电子测量技术 *
王光杓: "人全基因组测序在BGISEQ-500测序仪上的应用分析", 中国优秀硕士学位论文全文数据库 医药卫生科技辑 *

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