JP2017537615A5 - - Google Patents

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JP2017537615A5
JP2017537615A5 JP2017525396A JP2017525396A JP2017537615A5 JP 2017537615 A5 JP2017537615 A5 JP 2017537615A5 JP 2017525396 A JP2017525396 A JP 2017525396A JP 2017525396 A JP2017525396 A JP 2017525396A JP 2017537615 A5 JP2017537615 A5 JP 2017537615A5
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electric field
seconds
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cells
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JP2017525396A 2014-11-14 2015-11-13 破壊及び場による細胞への化合物及び組成物の送達 Active JP6846345B2 (ja)

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JP2021031352A JP7591265B2 (ja) 2014-11-14 2021-03-01 破壊及び場による細胞への化合物及び組成物の送達

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US201462080201P 2014-11-14 2014-11-14
US62/080,201 2014-11-14
US201562239241P 2015-10-08 2015-10-08
US62/239,241 2015-10-08
PCT/US2015/060689 WO2016077761A1 (en) 2014-11-14 2015-11-13 Disruption and field enabled delivery of compounds and compositions into cells

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JP2017537615A JP2017537615A (ja) 2017-12-21
JP2017537615A5 true JP2017537615A5 (enExample) 2018-12-27
JP6846345B2 JP6846345B2 (ja) 2021-03-24

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JP2017525396A Active JP6846345B2 (ja) 2014-11-14 2015-11-13 破壊及び場による細胞への化合物及び組成物の送達
JP2021031352A Active JP7591265B2 (ja) 2014-11-14 2021-03-01 破壊及び場による細胞への化合物及び組成物の送達
JP2023002190A Pending JP2023036990A (ja) 2014-11-14 2023-01-11 破壊及び場による細胞への化合物及び組成物の送達

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JP2023002190A Pending JP2023036990A (ja) 2014-11-14 2023-01-11 破壊及び場による細胞への化合物及び組成物の送達

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US (1) US10526573B2 (enExample)
EP (1) EP3218492A4 (enExample)
JP (3) JP6846345B2 (enExample)
CN (2) CN113897285A (enExample)
CA (1) CA2964138C (enExample)
WO (1) WO2016077761A1 (enExample)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013059343A1 (en) 2011-10-17 2013-04-25 Massachusetts Institute Of Technology Intracellular delivery
EP2934532B1 (en) 2012-12-20 2019-10-23 Purdue Research Foundation Chimeric antigen receptor-expressing t cells as anti-cancer therapeutics
US10124336B2 (en) 2013-08-16 2018-11-13 Massachusetts Institute Of Technology Selective delivery of material to cells
CN122564053A (zh) 2014-10-31 2026-08-14 麻省理工学院 递送生物分子至免疫细胞
WO2016077761A1 (en) 2014-11-14 2016-05-19 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
AU2016206870B2 (en) 2015-01-12 2022-02-17 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
US20180066223A1 (en) 2015-04-17 2018-03-08 Xcell Biosciences, Inc. Cancer cell enrichment system
JP6925984B2 (ja) 2015-07-09 2021-08-25 マサチューセッツ インスティテュート オブ テクノロジー 無核細胞への物質の送達
US11613759B2 (en) 2015-09-04 2023-03-28 Sqz Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
US20190275520A1 (en) 2016-03-31 2019-09-12 Massachusetts Institute Of Technology Flow-through microfluidic methods and devices featuring membrane-perturbing surface interactions for intracellular delivery
CA3019835A1 (en) 2016-04-08 2017-10-12 Purdue Research Foundation Methods and compositions for car t cell therapy
JP2019518009A (ja) 2016-05-03 2019-06-27 スクイーズ バイオテクノロジーズ カンパニー 寛容性を誘導するための生体分子の細胞内送達
CN109415741A (zh) 2016-05-03 2019-03-01 Sqz生物技术公司 细胞内递送生物分子以诱导耐受性
US10724043B2 (en) 2016-10-06 2020-07-28 Nantbio, Inc. Multi-pulse transfection methods and cells
EP3580556B1 (en) * 2017-02-13 2021-04-07 The Regents of The University of California Device and method for intracellular delivery of biomolecular cargo via acoustic wave exposure
CN110582288B (zh) 2017-02-28 2024-09-20 恩多塞特公司 用于car t细胞疗法的组合物和方法
EP3704257A4 (en) * 2017-11-02 2021-08-25 Indee. Pty. Ltd. INTRACELLULAR ADMINISTRATION AND RELATED PROCESS
US11365390B2 (en) 2017-12-19 2022-06-21 Xcell Biosciences, Inc. Methods of modulating cell phenotype by way of regulating the gaseous environment
WO2019144095A1 (en) 2018-01-22 2019-07-25 Seattle Children's Hospital (dba Seattle Children's Research Institute) Methods of use for car t cells
JP7568224B2 (ja) 2018-02-23 2024-10-16 エンドサイト・インコーポレイテッド Car t細胞療法のための配列決定法
MA52003A (fr) 2018-03-12 2021-01-20 Sqz Biotechnologies Co Procédés de traitement de maladies associées au hpv
EP3556845A1 (en) 2018-04-20 2019-10-23 Cellix Limited A method and device for transfecting cells
CN108887095B (zh) * 2018-05-15 2020-10-02 中亚民生科技发展有限公司 一种发光植物的培育方法
WO2020014538A1 (en) * 2018-07-12 2020-01-16 Gpb Scientific, Llc Microfluidic methods for the preparation of cells
WO2020041463A1 (en) * 2018-08-21 2020-02-27 The Regents Of The University Of California Lateral cavity acoustic transducer (lcat) for shear-induced cell transfection
US12478965B2 (en) 2018-08-21 2025-11-25 The Regents Of The University Of California Cavity acoustic transducer (CAT) for shear-induced cell transfection
WO2020047504A2 (en) * 2018-08-31 2020-03-05 The Charles Stark Draper Laboratory, Inc. Method and apparatus for high throughput high efficiency transfection of cells
EP3847234A2 (en) * 2018-09-04 2021-07-14 The Charles Stark Draper Laboratory, Inc. Method and device for high field strength electrotransfection of microvescicles and cells
CN111202506A (zh) * 2018-11-21 2020-05-29 浙江清华柔性电子技术研究院 流体的检测器件及其制备方法、血管中血液的检测器件
KR102232757B1 (ko) * 2018-11-22 2021-03-26 (주)엑솔런스바이오테크놀로지 체외충격파를 이용한 표적물질 전달 장치
WO2020154696A1 (en) 2019-01-25 2020-07-30 Sqz Biotechnologies Company Anucleate cell-derived vaccines
BR112021016903A2 (pt) 2019-02-28 2021-11-03 Sqz Biotechnologies Co Administração de biomoléculas a pbmcs para modificação de uma resposta imune
JP7353381B2 (ja) * 2019-03-12 2023-09-29 エムエックスティー バイオテック 細胞内物質伝達のためのマイクロ流体システムおよび方法
WO2020210162A1 (en) * 2019-04-08 2020-10-15 Sqz Biotechnologies Company Cartridge for use in a system for delivery of a payload into a cell
WO2020207456A1 (en) * 2019-04-11 2020-10-15 The Chinese University Of Hong Kong Systems and methods for controlled membrane disruption
US11685891B2 (en) 2019-05-17 2023-06-27 Regents Of The University Of Colorado Precise mechanical disruption for intracellular delivery to cells and small organisms
WO2020252215A1 (en) 2019-06-12 2020-12-17 Cellfe, Inc. Methods and systems for cell labeling and imaging
CN110272810A (zh) * 2019-07-01 2019-09-24 广州世赛生物科技有限公司 外源物质递送至真核细胞内的装置和方法及其应用
JP2022540797A (ja) * 2019-07-02 2022-09-20 カイトペン コーポレイション 電気機械的送達のためのデバイス、システム、及びキット、ならびにそれらの使用方法
WO2021194497A1 (en) 2020-03-26 2021-09-30 Hewlett-Packard Development Company, L.P. Cell preparation with a series of detection devices
CN112268934B (zh) * 2020-09-16 2022-06-28 东南大学 一种针对循环肿瘤细胞的检测芯片及其检测方法
IL302976A (en) 2020-11-18 2023-07-01 Cellfe Inc Mechanoporation-based methods and systems for high-throughput charge transfer into biological cells
US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
CN116670265B (zh) * 2020-12-29 2026-08-04 加拿大干细胞技术公司 用于扰动细胞膜的具有并行缩窄部的高通量微流控芯片
CN114134028B (zh) * 2022-01-29 2022-05-10 季华实验室 细胞显微注射装置和控制方法
CN115254214B (zh) * 2022-06-29 2023-07-25 中国科学院精密测量科学与技术创新研究院 一种微流控通道、微流控芯片和生化分子递送方法
CN115308301B (zh) * 2022-08-16 2023-03-10 中山大学 一种可测量细胞及细胞核的弹性模量的测量装置
CN115927465B (zh) * 2022-12-26 2025-06-17 苏州依科赛生物科技股份有限公司 一种真核细胞的电转染方法
EP4428222A1 (en) * 2023-03-10 2024-09-11 Cambridge Enterprise, Ltd. Devices and methods for electroporation
KR102679742B1 (ko) * 2023-11-30 2024-07-01 (주)엠엑스티바이오텍 액적 기반 세포 내 물질 전달 플랫폼

Family Cites Families (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2502621C3 (de) 1975-01-23 1978-09-14 Kernforschungsanlage Juelich Gmbh, 5170 Juelich Messung elastischer und dielektrischer Eigenschaften der Membran lebender Zellen
US4478824A (en) 1983-08-08 1984-10-23 Franco Robert S Method for altering red blood cell function and survival
FR2569477B1 (fr) 1984-08-24 1987-01-02 Descartes Universite Rene Appareil et procede pour la determination de la deformabilite des globules rouges du sang
DE3742607A1 (de) 1987-12-16 1989-06-29 Asea Brown Boveri Hochtemperatur-speicherbatterie
JP2720161B2 (ja) 1988-02-01 1998-02-25 株式会社アドバンス 細胞変形能測定装置
JP2685544B2 (ja) 1988-11-11 1997-12-03 株式会社日立製作所 血液フィルタおよび血液検査方法並びに血液検査装置
JP2532707B2 (ja) 1990-03-08 1996-09-11 佑二 菊池 血液回路及びこれを用いた血液測定装置及び血液測定方法
ATE162402T1 (de) 1990-04-24 1998-02-15 Flustat Pty Ltd Oraler an der oberfläche von erythrozyten gebundene antigene beinhaltender impfstoff
US5658892A (en) 1993-01-15 1997-08-19 The General Hospital Corporation Compound delivery using high-pressure impulse transients
US6218166B1 (en) 1994-12-09 2001-04-17 John Wayne Cancer Institute Adjuvant incorporation into antigen carrying cells: compositions and methods
WO1996027392A1 (en) 1995-03-08 1996-09-12 The Scripps Research Institute Antigen presenting system and methods for activation of t-cells
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
EP0874635B1 (en) 1995-12-04 2003-03-19 Puget Sound Blood Center Nonimmunogenic platelet and red blood cell compositions
AU734196B2 (en) 1996-03-28 2001-06-07 Whitehead Institute Opsonin-enhanced cells, and methods of modulating an immune response to an antigen
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
EP0882448B1 (en) 1997-05-05 2005-01-12 DIDECO S.r.l. Method of encapsulating biologically active agents within erythrocytes and apparatus therefor
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
SE9704076D0 (sv) 1997-11-06 1997-11-06 Holdingbolaget Vid Goeteborgs Method for permeabilisation of cell structures and use thereof
GB9816583D0 (en) 1998-07-31 1998-09-30 Univ Ulster Nucleic acid carrier
AU5757100A (en) 1999-06-21 2001-01-09 General Hospital Corporation, The Methods and devices for cell culturing and organ assist systems
JP2002325572A (ja) 2000-12-25 2002-11-12 Univ Osaka 外来物質の導入方法
AU2002252073A1 (en) 2001-02-22 2002-09-12 The Scepens Eye Research Institute, Inc. Tolerogenic antigen presenting cells and in treating immune-inflammatory conditions
WO2003020039A1 (en) 2001-08-28 2003-03-13 Rush-Presbyterian-St. Luke's Medical Center Immune tolerance to predetermined antigens
AU2002353741A1 (en) 2001-11-27 2003-06-10 Cellectricon Ab A method for combined sequential agent delivery and electroporation for cell structures and use thereof
US20030133922A1 (en) 2002-01-15 2003-07-17 Kasha John R. Oral tolerance using allogeneic platelets in ITP
US7148059B1 (en) * 2002-02-22 2006-12-12 Ionoptix Corporation Myocyte culture pacing apparatus
WO2003104788A1 (ja) 2002-06-05 2003-12-18 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
GB0214528D0 (en) 2002-06-24 2002-08-07 Univ Aberdeen Materials and methods for induction of immune tolerance
CA2513072A1 (en) 2003-01-09 2004-07-29 Invitrogen Corporation Cellular delivery and activation polypeptide-nucleic acid complexes
US20060134067A1 (en) 2003-02-18 2006-06-22 Maxcyte, Inc. Loading of cells with antigens by electroporation
CN1981073B (zh) 2004-07-27 2011-07-13 帝斯曼知识产权资产管理有限公司 用于制备碳纳米管/超高摩尔质量聚乙烯复合纤维的方法
US20060134772A1 (en) 2004-11-18 2006-06-22 The Regents Of The University Of California System for locating cells and for cellular analysis
WO2006095330A2 (en) 2005-03-10 2006-09-14 Yeda Research And Development Co. Ltd. Methods and immunogenic cell preparations for treating antigen-associated diseases
US7704743B2 (en) 2005-03-30 2010-04-27 Georgia Tech Research Corporation Electrosonic cell manipulation device and method of use thereof
US7993821B2 (en) 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
US9005974B2 (en) 2005-12-09 2015-04-14 Academish Medisch Centrum Bij De Universiteit Van Amsterdam Means and methods for influencing the stability of cells
WO2007097934A2 (en) 2006-02-17 2007-08-30 Elusys Therapeutics, Inc. Methods and compositions for using erythrocytes as carriers for delivery of drugs
US8293524B2 (en) 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
US20070249038A1 (en) 2006-04-21 2007-10-25 Andrea Adamo Microfluidic device for single cell targeted operations
AU2007284454A1 (en) 2006-08-17 2008-02-21 Massachusetts Institute Of Technology Method and apparatus for microfluidic injection
US20120264108A1 (en) * 2007-01-05 2012-10-18 The Regents Of The University Of California Intracellular Molecular Delivery Based On Nanostructure Injectors
US20080311140A1 (en) 2007-05-29 2008-12-18 Baylor College Of Medicine Antigen specific immunosuppression by dendritic cell therapy
US8841135B2 (en) 2007-06-20 2014-09-23 University Of Washington Biochip for high-throughput screening of circulating tumor cells
EP2169070B1 (en) 2007-06-21 2015-06-10 National University Corporation Nagoya University Method for introducing foreign substance into cell having cell wall
FR2919804B1 (fr) 2007-08-08 2010-08-27 Erytech Pharma Composition et vaccin therapeutique anti-tumoral
GB0718160D0 (en) 2007-09-18 2007-10-24 Medical Res Council Methods
EP2057998A1 (en) 2007-10-31 2009-05-13 Universitätsklinikum Hamburg-Eppendorf Use of modified cells for the treatment of multiple sclerosis
CN101874201B (zh) 2007-11-28 2012-08-15 柯尼卡美能达精密光学株式会社 血液流动性测量装置及血液流动性测量方法
US20090280518A1 (en) * 2008-05-12 2009-11-12 Massachusetts Institute Of Technology System for high throughput measurement of mechanical properties of cells
JP5137129B2 (ja) 2008-07-23 2013-02-06 国立大学法人山口大学 血液細胞の力学的特性測定装置
EP2321405A4 (en) 2008-08-08 2012-09-12 Agency Science Tech & Res MICROFLUIDIC CONTINUOUS FLOW DEVICE
US8211656B2 (en) 2008-08-13 2012-07-03 The Invention Science Fund I, Llc Biological targeting compositions and methods of using the same
US8187864B2 (en) * 2008-10-01 2012-05-29 The Governing Council Of The University Of Toronto Exchangeable sheets pre-loaded with reagent depots for digital microfluidics
US20110091973A1 (en) 2008-12-09 2011-04-21 Glaser Larry F Modified and fusion enhanced erythrocytes, cells and uses thereof
US9157550B2 (en) 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
US9017991B2 (en) 2009-03-13 2015-04-28 Tufts University Methods tip assemblies and kits for introducing material into cells
EP2405959A4 (en) 2009-03-13 2013-10-16 Univ Tufts METHODS, DEVICES AND KITS FOR INTRODUCING GENETIC MATERIAL IN LIVING CELLS
US20120107925A1 (en) 2009-03-20 2012-05-03 Mo Huang Li Devices for separating cells and methods of using them
WO2010129671A2 (en) * 2009-05-08 2010-11-11 Chauncey Sayre Method of and apparatus for changing one type of cell into another type of cell
GB0909754D0 (en) 2009-06-05 2009-07-22 Magnani Mauro Drug delivery systems
EP2451475A2 (en) 2009-07-06 2012-05-16 Novartis AG Self replicating rna molecules and uses thereof
US9364831B2 (en) * 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
ES2600932T3 (es) 2009-10-27 2017-02-13 Erytech Pharma Composición para inducir una tolerancia inmunitaria específica
US20130023051A1 (en) 2009-12-21 2013-01-24 Keygene N.V. Techniques for transfecting protoplasts
WO2011084882A2 (en) 2010-01-05 2011-07-14 Contrafect Corporation Methods and compositions for enhanced immunological therapy and targeting of gram-positive bacteria
US8844570B2 (en) 2010-02-01 2014-09-30 California Institute Of Technology Generating binary states using a microfluidic channel
JP5704590B2 (ja) 2010-02-05 2015-04-22 国立大学法人東京農工大学 サイズ選択マイクロキャビティアレイを用いた循環腫瘍細胞の検出
US20110289043A1 (en) 2010-03-22 2011-11-24 Brown University Research Foundation Computational methods and compositions
CA2825093A1 (en) 2011-01-19 2012-07-26 The University Of British Columbia Apparatus and method for particle separation
EP2681306B1 (en) 2011-02-28 2019-01-09 President and Fellows of Harvard College Cell culture system
CN102250842A (zh) * 2011-05-23 2011-11-23 昆明理工大学 一种电穿孔转染昆虫细胞的方法
CN116179323A (zh) 2011-05-27 2023-05-30 不列颠哥伦比亚大学 用于高通量分析的微流控细胞捕获和分析设备
HRP20141230T1 (hr) 2011-07-05 2015-02-13 SOTIO a.s. Naäśini i postupci aktivne staniäśne imunoterapije raka upotrebom tumorskih stanica usmrä†enih visokim hidrostatskim tlakom, te dendritiäśkih stanica
US20130065314A1 (en) 2011-08-25 2013-03-14 Phytocentric Biotech Inc. Algal transformation systems, compositions and methods
WO2013059343A1 (en) * 2011-10-17 2013-04-25 Massachusetts Institute Of Technology Intracellular delivery
CN104684546A (zh) 2012-06-07 2015-06-03 哈佛大学校长及研究员协会 用于药物靶向的纳米疗法
US9255245B2 (en) 2012-07-03 2016-02-09 Agilent Technologies, Inc. Sample probes and methods for sampling intracellular material
US9327526B2 (en) * 2012-07-25 2016-05-03 Xerox Corporation Active biased electrodes for reducing electrostatic fields underneath print heads in an electrostatic media transport
CN102925337B (zh) 2012-11-08 2014-06-18 武汉友芝友生物制药有限公司 一种微流体细胞捕获芯片及其制备方法
GB201300049D0 (en) 2013-01-03 2013-02-20 Transimmune Ag Method for obtaining immuno-stimulatory dendritic cells
GB201300052D0 (en) 2013-01-03 2013-02-20 Transimmune Ag Method for obtaining immuno-suppressive dendritic cells
WO2014120956A1 (en) 2013-01-30 2014-08-07 Asta Fluidic Technologies, Inc. Microfluidic-based fetal red blood cell detection
US9725709B2 (en) 2013-03-12 2017-08-08 OpenCell Technologies, Inc. Intracellular delivery and transfection methods and devices
WO2014151888A1 (en) 2013-03-15 2014-09-25 The Regents Of The University Of California High-throughput cargo delivery into live cells using photothermal platforms
KR20140115560A (ko) 2013-03-21 2014-10-01 인하대학교 산학협력단 기계적 스트레스를 이용한 단세포 생물체 개질 방법 및 이에 사용되는 장치
EP3789487A1 (en) 2013-04-03 2021-03-10 Memorial Sloan Kettering Cancer Center Effective generation of tumor-targeted t-cells derived from pluripotent stem cells
US10124336B2 (en) 2013-08-16 2018-11-13 Massachusetts Institute Of Technology Selective delivery of material to cells
CA2927947A1 (en) 2013-10-22 2015-04-30 Cellanyx Diagnostics, Llc Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells
US10137673B2 (en) 2013-12-31 2018-11-27 Canon U.S. Life Sciences, Inc. Methods and systems for continuous flow cell lysis in a microfluidic device
CN106456744A (zh) 2014-04-01 2017-02-22 鲁比厄斯治疗法股份有限公司 用于免疫调节的方法和组合物
SG11201608701QA (en) 2014-04-18 2016-11-29 Editas Medicine Inc Crispr-cas-related methods, compositions and components for cancer immunotherapy
WO2016003485A1 (en) * 2014-07-03 2016-01-07 Massachusetts Institute Of Technology Microfluidic assay for rapid optimization of cell electroporation
CN122564053A (zh) 2014-10-31 2026-08-14 麻省理工学院 递送生物分子至免疫细胞
WO2016077761A1 (en) 2014-11-14 2016-05-19 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
EP3242946B1 (en) 2015-01-07 2026-08-19 Indee. Inc. A method for mechanical and hydrodynamic microfluidic transfection and apparatus therefor
AU2016206870B2 (en) 2015-01-12 2022-02-17 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
WO2016183482A1 (en) 2015-05-13 2016-11-17 Rubius Therapeutics, Inc. Membrane-receiver complex therapeutics
GB201513278D0 (en) 2015-07-03 2015-09-09 Transimmune Ag And Yale University Device and method for obtaining immunostimulatory antigen-presenting cells
JP6925984B2 (ja) 2015-07-09 2021-08-25 マサチューセッツ インスティテュート オブ テクノロジー 無核細胞への物質の送達
US20190382796A1 (en) 2015-09-04 2019-12-19 Sqz Biotechnologies Company Intracellular delivery of biomolecules mediated by a surface with pores
US11613759B2 (en) 2015-09-04 2023-03-28 Sqz Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
AU2016374643B2 (en) 2015-12-22 2023-12-14 Sangui Bio Pty. Ltd Therapeutic methods using erythrocytes
CN108697733A (zh) 2016-01-11 2018-10-23 鲁比厄斯治疗法股份有限公司 与癌症适应症的多模式治疗性细胞体系有关的组合物和方法
EP3402890B1 (en) 2016-01-12 2025-01-01 SQZ Biotechnologies Company Intracellular delivery of complexes
JP2019518009A (ja) 2016-05-03 2019-06-27 スクイーズ バイオテクノロジーズ カンパニー 寛容性を誘導するための生体分子の細胞内送達
CN109415741A (zh) 2016-05-03 2019-03-01 Sqz生物技术公司 细胞内递送生物分子以诱导耐受性
US20170326213A1 (en) 2016-05-16 2017-11-16 Augusta University Research Institute, Inc. Protein-Coupled Red Blood Cell Compositions and Methods of Their Use
CN106244543A (zh) 2016-07-29 2016-12-21 北京时合生物科技有限公司 一种pbmc体外诱导分化成为树突状细胞的方法
WO2018089497A1 (en) 2016-11-08 2018-05-17 Georgia Tech Research Corporation Methods for convectively-driven intracellular delivery

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