JP2018500913A5 - - Google Patents
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- JP2018500913A5 JP2018500913A5 JP2017534913A JP2017534913A JP2018500913A5 JP 2018500913 A5 JP2018500913 A5 JP 2018500913A5 JP 2017534913 A JP2017534913 A JP 2017534913A JP 2017534913 A JP2017534913 A JP 2017534913A JP 2018500913 A5 JP2018500913 A5 JP 2018500913A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- nucleic acid
- cell
- cells
- rnp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004027 cell Anatomy 0.000 claims 36
- 108090000623 proteins and genes Proteins 0.000 claims 29
- 102000004169 proteins and genes Human genes 0.000 claims 27
- 238000000034 method Methods 0.000 claims 25
- 150000007523 nucleic acids Chemical class 0.000 claims 18
- 108020004707 nucleic acids Proteins 0.000 claims 14
- 102000039446 nucleic acids Human genes 0.000 claims 14
- 108010081734 Ribonucleoproteins Proteins 0.000 claims 13
- 102000004389 Ribonucleoproteins Human genes 0.000 claims 13
- 208000031481 Pathologic Constriction Diseases 0.000 claims 6
- 208000037804 stenosis Diseases 0.000 claims 6
- 230000036262 stenosis Effects 0.000 claims 6
- 239000000725 suspension Substances 0.000 claims 5
- 108020004414 DNA Proteins 0.000 claims 4
- 230000014509 gene expression Effects 0.000 claims 4
- 108091079001 CRISPR RNA Proteins 0.000 claims 2
- 108010051219 Cre recombinase Proteins 0.000 claims 2
- 108091027544 Subgenomic mRNA Proteins 0.000 claims 2
- 238000010459 TALEN Methods 0.000 claims 2
- 108091028113 Trans-activating crRNA Proteins 0.000 claims 2
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims 2
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 2
- -1 meganuclease Proteins 0.000 claims 2
- 108091033409 CRISPR Proteins 0.000 claims 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims 1
- 108020005004 Guide RNA Proteins 0.000 claims 1
- 102000006479 Heterogeneous-Nuclear Ribonucleoproteins Human genes 0.000 claims 1
- 108010019372 Heterogeneous-Nuclear Ribonucleoproteins Proteins 0.000 claims 1
- 108010033040 Histones Proteins 0.000 claims 1
- 102000006947 Histones Human genes 0.000 claims 1
- 108090000621 Ribonuclease P Proteins 0.000 claims 1
- 102000004167 Ribonuclease P Human genes 0.000 claims 1
- 108010003165 Small Nuclear Ribonucleoproteins Proteins 0.000 claims 1
- 102000004598 Small Nuclear Ribonucleoproteins Human genes 0.000 claims 1
- 108010017842 Telomerase Proteins 0.000 claims 1
- 210000000349 chromosome Anatomy 0.000 claims 1
- 230000000536 complexating effect Effects 0.000 claims 1
- 238000010362 genome editing Methods 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 210000004940 nucleus Anatomy 0.000 claims 1
- 210000003705 ribosome Anatomy 0.000 claims 1
- 150000003384 small molecules Chemical class 0.000 claims 1
- 230000002459 sustained effect Effects 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021087763A JP2021129584A (ja) | 2015-01-12 | 2021-05-25 | マイクロ流体送達による遺伝子編集 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562102347P | 2015-01-12 | 2015-01-12 | |
| US62/102,347 | 2015-01-12 | ||
| PCT/US2016/013113 WO2016115179A1 (en) | 2015-01-12 | 2016-01-12 | Gene editing through microfluidic delivery |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021087763A Division JP2021129584A (ja) | 2015-01-12 | 2021-05-25 | マイクロ流体送達による遺伝子編集 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018500913A JP2018500913A (ja) | 2018-01-18 |
| JP2018500913A5 true JP2018500913A5 (enExample) | 2019-02-28 |
| JP7278027B2 JP7278027B2 (ja) | 2023-05-19 |
Family
ID=56406306
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017534913A Active JP7278027B2 (ja) | 2015-01-12 | 2016-01-12 | マイクロ流体送達による遺伝子編集 |
| JP2021087763A Pending JP2021129584A (ja) | 2015-01-12 | 2021-05-25 | マイクロ流体送達による遺伝子編集 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021087763A Pending JP2021129584A (ja) | 2015-01-12 | 2021-05-25 | マイクロ流体送達による遺伝子編集 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US11125739B2 (enExample) |
| EP (1) | EP3245294A4 (enExample) |
| JP (2) | JP7278027B2 (enExample) |
| CN (1) | CN107250373A (enExample) |
| AU (1) | AU2016206870B2 (enExample) |
| WO (1) | WO2016115179A1 (enExample) |
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| BR112014009346B1 (pt) * | 2011-10-17 | 2020-09-15 | Massachusetts Institute Of Technology | Entrega intracelular |
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| KR102243597B1 (ko) | 2013-08-16 | 2021-04-26 | 메사추세츠 인스티튜트 오브 테크놀로지 | 세포로의 선택적 물질 전달 |
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| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
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| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
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| CN107002089B (zh) | 2014-11-14 | 2021-09-07 | 麻省理工学院 | 化合物和组合物向细胞中的破坏和场实现的递送 |
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2016
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- 2016-01-12 AU AU2016206870A patent/AU2016206870B2/en active Active
- 2016-01-12 CN CN201680008278.0A patent/CN107250373A/zh active Pending
- 2016-01-12 US US15/542,892 patent/US11125739B2/en active Active
- 2016-01-12 JP JP2017534913A patent/JP7278027B2/ja active Active
- 2016-01-12 EP EP16737769.6A patent/EP3245294A4/en active Pending
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2021
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- 2021-08-17 US US17/404,286 patent/US12130281B2/en active Active
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