JP2017503521A5 - - Google Patents
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- Publication number
- JP2017503521A5 JP2017503521A5 JP2016548054A JP2016548054A JP2017503521A5 JP 2017503521 A5 JP2017503521 A5 JP 2017503521A5 JP 2016548054 A JP2016548054 A JP 2016548054A JP 2016548054 A JP2016548054 A JP 2016548054A JP 2017503521 A5 JP2017503521 A5 JP 2017503521A5
- Authority
- JP
- Japan
- Prior art keywords
- reverse transcriptase
- nucleic acid
- mutant
- mlv
- mlv reverse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102100034343 Integrase Human genes 0.000 claims description 190
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 187
- 230000000694 effects Effects 0.000 claims description 46
- 102000039446 nucleic acids Human genes 0.000 claims description 44
- 108020004707 nucleic acids Proteins 0.000 claims description 44
- 150000007523 nucleic acids Chemical class 0.000 claims description 44
- 238000000034 method Methods 0.000 claims description 32
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 31
- 230000035772 mutation Effects 0.000 claims description 27
- 239000000203 mixture Substances 0.000 claims description 23
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 16
- 230000000295 complement effect Effects 0.000 claims description 12
- 239000011692 calcium ascorbate Substances 0.000 claims description 9
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
- 238000001668 nucleic acid synthesis Methods 0.000 claims description 7
- 239000002773 nucleotide Substances 0.000 claims description 7
- 125000003729 nucleotide group Chemical group 0.000 claims description 7
- 108020004999 messenger RNA Proteins 0.000 claims description 6
- 102200054079 rs121907976 Human genes 0.000 claims description 6
- 102200070544 rs202198133 Human genes 0.000 claims description 6
- 102200111286 rs2234704 Human genes 0.000 claims description 6
- 102220242537 rs762217448 Human genes 0.000 claims description 6
- 102200078515 rs779270933 Human genes 0.000 claims description 6
- 239000005546 dideoxynucleotide Substances 0.000 claims description 4
- 229920001184 polypeptide Polymers 0.000 claims description 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 238000010839 reverse transcription Methods 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- 101710203526 Integrase Proteins 0.000 claims description 3
- 239000013604 expression vector Substances 0.000 claims description 2
- 102200028520 rs1057520297 Human genes 0.000 claims description 2
- 239000013598 vector Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 description 15
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000004113 Sepiolite Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 239000003419 rna directed dna polymerase inhibitor Substances 0.000 description 2
- 102220288227 rs141863326 Human genes 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461930395P | 2014-01-22 | 2014-01-22 | |
| US61/930,395 | 2014-01-22 | ||
| PCT/US2015/012534 WO2015112767A2 (en) | 2014-01-22 | 2015-01-22 | Novel reverse transcriptases for use in high temperature nucleic acid synthesis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017503521A JP2017503521A (ja) | 2017-02-02 |
| JP2017503521A5 true JP2017503521A5 (enExample) | 2018-02-01 |
Family
ID=52444678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016548054A Pending JP2017503521A (ja) | 2014-01-22 | 2015-01-22 | 高温核酸合成で使用するための新規な逆転写酵素 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9663770B2 (enExample) |
| EP (1) | EP3097190A2 (enExample) |
| JP (1) | JP2017503521A (enExample) |
| KR (1) | KR20160113177A (enExample) |
| CN (1) | CN106164261A (enExample) |
| WO (1) | WO2015112767A2 (enExample) |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| EP3186374A1 (en) * | 2014-08-25 | 2017-07-05 | Life Technologies Corporation | Reversibly inactivated thermostable reverse transcriptases, compositions and methods for use |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US9580698B1 (en) * | 2016-09-23 | 2017-02-28 | New England Biolabs, Inc. | Mutant reverse transcriptase |
| EP3940066A1 (en) | 2016-09-23 | 2022-01-19 | New England Biolabs, Inc. | A mutant reverse transcriptase |
| WO2018110595A1 (ja) | 2016-12-14 | 2018-06-21 | タカラバイオ株式会社 | 耐熱性の逆転写酵素変異体 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| ES2880366T3 (es) | 2017-04-11 | 2021-11-24 | Hoffmann La Roche | Retrotranscriptasa mutante con un incremento en la estabilidad térmica así como productos, procedimientos y usos que involucran la misma |
| US20180312822A1 (en) * | 2017-04-26 | 2018-11-01 | 10X Genomics, Inc. | Mmlv reverse transcriptase variants |
| EP3592847B1 (en) * | 2017-04-26 | 2024-06-05 | 10X Genomics, Inc. | Mmlv reverse transcriptase variants |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| WO2020132966A1 (zh) * | 2018-12-26 | 2020-07-02 | 深圳华大生命科学研究院 | 酶活性提高的逆转录酶及其应用 |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001306T5 (de) * | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CA3148761A1 (en) * | 2019-07-26 | 2021-02-04 | Becton, Dickinson And Company | Buffer compositions for reducing aggregation |
| CN114174503B (zh) * | 2019-07-26 | 2024-06-28 | 东洋纺株式会社 | 稳定性优异的突变型逆转录酶 |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| CN112795547B (zh) * | 2019-11-13 | 2024-05-31 | 广州达安基因股份有限公司 | 高逆转录效率的逆转录酶突变体 |
| CN112795551B (zh) * | 2019-11-13 | 2024-01-30 | 广州达安基因股份有限公司 | 一种耐高温逆转录酶突变体及其应用 |
| CN112795550B (zh) * | 2019-11-13 | 2024-05-31 | 广州达安基因股份有限公司 | 耐高温逆转录酶突变体 |
| CN112795546B (zh) * | 2019-11-13 | 2024-02-27 | 广州达安基因股份有限公司 | 一种具有高逆转录效率的耐高温逆转录酶突变体及其应用 |
| CN112795549B (zh) * | 2019-11-13 | 2023-11-28 | 广州达安基因股份有限公司 | 一种逆转录酶突变体 |
| EP4073241A2 (en) | 2019-12-11 | 2022-10-19 | 10X Genomics, Inc. | Reverse transcriptase variants |
| CA3167722A1 (en) * | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Reverse transcription during template emulsification |
| US11827936B2 (en) | 2020-01-13 | 2023-11-28 | Fluent Biosciences Inc. | Methods and systems for single cell gene profiling |
| WO2021146184A1 (en) | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Single cell sequencing |
| EP4090328A4 (en) | 2020-01-13 | 2024-02-14 | Fluent Biosciences Inc. | EMULSION-BASED ACTIVE SCREENING |
| JP2023515499A (ja) * | 2020-02-21 | 2023-04-13 | バイオオー サイエンティフィック コーポレーション | 超並列核酸シーケンシングライブラリを生成するための組成物及び方法 |
| AU2021230546A1 (en) * | 2020-03-04 | 2022-10-13 | Flagship Pioneering Innovations Vi, Llc | Methods and compositions for modulating a genome |
| CA3175931A1 (en) | 2020-03-16 | 2021-09-23 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
| US12428685B2 (en) | 2020-03-24 | 2025-09-30 | Illumina, Inc. | Viral detection using template emulsification |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
| CN111647576B (zh) * | 2020-06-24 | 2021-02-09 | 南京诺唯赞生物科技股份有限公司 | 一种热稳定性逆转录酶突变体及其应用 |
| KR102338690B1 (ko) * | 2020-07-09 | 2021-12-14 | (주)바이오닉스 | 항체결합단백질 및 우라실 dna 글리코실레이즈 효소를 포함하는 융합 단백질, 및 상기 융합 단백질의 용도 |
| JP2023534028A (ja) | 2020-07-15 | 2023-08-07 | フルーエント バイオサイエンシーズ インコーポレイテッド | 段階的ライゲーション用オリゴ |
| CA3186660A1 (en) * | 2020-07-20 | 2022-01-27 | Sarah Franz BEAUDOIN | Reverse transcriptase mutants with increased activity and thermostability |
| US20220135955A1 (en) * | 2020-07-20 | 2022-05-05 | Integrated DNA Techonolgies, Inc. | Reverse Transcriptase Mutants with Increased Activity and Thermostability |
| CN114480338B (zh) * | 2020-11-13 | 2024-04-05 | 广州达安基因股份有限公司 | 用于核酸检测的逆转录酶突变体 |
| CN114480330B (zh) * | 2020-11-13 | 2024-11-01 | 广州达安基因股份有限公司 | 多突变位点的逆转录酶突变体 |
| CN114480335B (zh) * | 2020-11-13 | 2024-06-07 | 广州达安基因股份有限公司 | 逆转录酶及逆转录检测试剂 |
| CN114480331A (zh) * | 2020-11-13 | 2022-05-13 | 广州达安基因股份有限公司 | Mmlv酶组合突变体 |
| CN112251423B (zh) * | 2020-12-08 | 2021-03-19 | 北京健为医学检验实验室有限公司 | 一种m-mlv逆转录酶体及其应用 |
| CN113234858A (zh) * | 2021-05-20 | 2021-08-10 | 阿吉安(福州)基因医学检验实验室有限公司 | 一种covid-19的检测试剂盒 |
| CN113355303B (zh) * | 2021-06-07 | 2023-06-16 | 中元汇吉生物技术股份有限公司 | 一种m-mlv逆转录酶突变体及其应用 |
| CA3231677A1 (en) * | 2021-09-08 | 2023-03-16 | Flagship Pioneering Innovations Vi, Llc | Methods and compositions for modulating a genome |
| CN117586983A (zh) * | 2022-08-11 | 2024-02-23 | 菲鹏生物股份有限公司 | 一种逆转录酶、核酸分子及合成cDNA的方法 |
| WO2024137911A1 (en) * | 2022-12-21 | 2024-06-27 | Pairwise Plants Services, Inc. | Engineered proteins and methods of use thereof |
| CN116004568B (zh) * | 2023-02-07 | 2025-10-17 | 北京诺派生物科技有限公司 | 一种可用于核酸检测的耐高温反转录酶及其制备方法与应用 |
| CN116286721A (zh) * | 2023-03-29 | 2023-06-23 | 江苏同科医药科技有限公司 | 一种耐热型逆转录酶突变体及其制备方法和应用 |
| CN116286723A (zh) * | 2023-04-28 | 2023-06-23 | 深圳市艾伟迪生物科技有限公司 | 一种基于蛋白定向进化的快速逆转录酶及其应用 |
| GB2630305A (en) * | 2023-05-22 | 2024-11-27 | Thermo Fisher Scientific Baltics Uab | Reverse transcriptase with improved properties |
| CN116694598B (zh) * | 2023-06-30 | 2024-12-13 | 翌圣生物科技(上海)股份有限公司 | 一种鼠白血病病毒逆转录酶突变体及其应用 |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| DE229810T1 (de) | 1985-07-09 | 1987-11-05 | Quadrant Bioresources Ltd., Soulbury, Leighton Buzzard, Bedfordshire | Beschuetzung von proteinen und aehnlichem. |
| US5618711A (en) | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5561058A (en) | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5693517A (en) | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
| US4962022A (en) | 1986-09-22 | 1990-10-09 | Becton Dickinson And Company | Storage and use of liposomes |
| US5244797B1 (en) | 1988-01-13 | 1998-08-25 | Life Technologies Inc | Cloned genes encoding reverse transcriptase lacking rnase h activity |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5498523A (en) | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
| GB8903593D0 (en) | 1989-02-16 | 1989-04-05 | Pafra Ltd | Storage of materials |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| US5646016A (en) | 1991-02-06 | 1997-07-08 | Genetics Institute, Inc. | Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules |
| HU223760B1 (hu) | 1991-09-24 | 2005-01-28 | Keygene N.V. | Szelektív restrikciós fragmentumsokszorosítás, általános módszer DNS-fingerprint analízisére |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| US6015668A (en) | 1994-09-30 | 2000-01-18 | Life Technologies, Inc. | Cloned DNA polymerases from thermotoga and mutants thereof |
| US5912155A (en) | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
| US5556771A (en) | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| WO1997009451A1 (en) | 1995-09-08 | 1997-03-13 | Life Technologies, Inc. | Cloned dna polymerases from thermotoga and mutants thereof |
| US6013488A (en) | 1996-07-25 | 2000-01-11 | The Institute Of Physical And Chemical Research | Method for reverse transcription |
| WO1998023733A2 (en) | 1996-11-27 | 1998-06-04 | University Of Washington | Thermostable polymerases having altered fidelity |
| US6989259B2 (en) | 1997-04-22 | 2006-01-24 | Invitrogen Corporation | Recombinant methods for making reverse transcriptases and mutants thereof |
| US6183998B1 (en) | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
| JP4193079B2 (ja) | 1998-11-10 | 2008-12-10 | 東洋紡績株式会社 | 変異型逆転写酵素 |
| EP1266024A4 (en) | 2000-03-15 | 2003-07-30 | Invitrogen Corp | HIGH FIDELITY INVERTED TRANSCRIPTASES AND USES THEREOF |
| EP2295551B1 (en) * | 2000-05-26 | 2016-04-06 | Life Technologies Corporation | Thermostable reverse transcriptases and uses thereof |
| US9771565B2 (en) * | 2000-05-26 | 2017-09-26 | Life Technologies Corporation | Thermostable reverse transcriptases and uses thereof |
| US7078208B2 (en) | 2000-05-26 | 2006-07-18 | Invitrogen Corporation | Thermostable reverse transcriptases and uses thereof |
| JP2005508630A (ja) | 2001-09-14 | 2005-04-07 | インヴィトロジェン コーポレーション | Ｄｎａポリメラーゼとその変異体 |
| EP2325303B1 (en) | 2002-09-13 | 2013-11-06 | Life Technologies Corporation | Thermostable reverse transcriptases and uses thereof |
| US7579153B2 (en) | 2005-01-25 | 2009-08-25 | Population Genetics Technologies, Ltd. | Isothermal DNA amplification |
| ATE535603T1 (de) | 2005-08-10 | 2011-12-15 | Stratagene California | Mutante reverse transkriptase und verwendungsverfahren |
| GB0806562D0 (en) * | 2008-04-10 | 2008-05-14 | Fermentas Uab | Production of nucleic acid |
| US8900814B2 (en) * | 2010-08-13 | 2014-12-02 | Kyoto University | Variant reverse transcriptase |
-
2015
- 2015-01-22 CN CN201580014630.7A patent/CN106164261A/zh active Pending
- 2015-01-22 US US14/603,256 patent/US9663770B2/en active Active
- 2015-01-22 KR KR1020167022831A patent/KR20160113177A/ko not_active Withdrawn
- 2015-01-22 JP JP2016548054A patent/JP2017503521A/ja active Pending
- 2015-01-22 WO PCT/US2015/012534 patent/WO2015112767A2/en not_active Ceased
- 2015-01-22 EP EP15702365.6A patent/EP3097190A2/en not_active Withdrawn
-
2017
- 2017-05-26 US US15/607,247 patent/US20180010105A1/en not_active Abandoned
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