JP2017503521A5 - - Google Patents
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- JP2017503521A5 JP2017503521A5 JP2016548054A JP2016548054A JP2017503521A5 JP 2017503521 A5 JP2017503521 A5 JP 2017503521A5 JP 2016548054 A JP2016548054 A JP 2016548054A JP 2016548054 A JP2016548054 A JP 2016548054A JP 2017503521 A5 JP2017503521 A5 JP 2017503521A5
- Authority
- JP
- Japan
- Prior art keywords
- reverse transcriptase
- nucleic acid
- mutant
- mlv
- mlv reverse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102100034343 Integrase Human genes 0.000 claims description 190
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 187
- 230000000694 effects Effects 0.000 claims description 46
- 102000039446 nucleic acids Human genes 0.000 claims description 44
- 108020004707 nucleic acids Proteins 0.000 claims description 44
- 150000007523 nucleic acids Chemical class 0.000 claims description 44
- 238000000034 method Methods 0.000 claims description 32
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 31
- 230000035772 mutation Effects 0.000 claims description 27
- 239000000203 mixture Substances 0.000 claims description 23
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 16
- 230000000295 complement effect Effects 0.000 claims description 12
- 239000011692 calcium ascorbate Substances 0.000 claims description 9
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
- 238000001668 nucleic acid synthesis Methods 0.000 claims description 7
- 239000002773 nucleotide Substances 0.000 claims description 7
- 125000003729 nucleotide group Chemical group 0.000 claims description 7
- 108020004999 messenger RNA Proteins 0.000 claims description 6
- 102200054079 rs121907976 Human genes 0.000 claims description 6
- 102200070544 rs202198133 Human genes 0.000 claims description 6
- 102200111286 rs2234704 Human genes 0.000 claims description 6
- 102220242537 rs762217448 Human genes 0.000 claims description 6
- 102200078515 rs779270933 Human genes 0.000 claims description 6
- 239000005546 dideoxynucleotide Substances 0.000 claims description 4
- 229920001184 polypeptide Polymers 0.000 claims description 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 238000010839 reverse transcription Methods 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- 101710203526 Integrase Proteins 0.000 claims description 3
- 239000013604 expression vector Substances 0.000 claims description 2
- 102200028520 rs1057520297 Human genes 0.000 claims description 2
- 239000013598 vector Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 description 15
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000004113 Sepiolite Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 239000003419 rna directed dna polymerase inhibitor Substances 0.000 description 2
- 102220288227 rs141863326 Human genes 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461930395P | 2014-01-22 | 2014-01-22 | |
| US61/930,395 | 2014-01-22 | ||
| PCT/US2015/012534 WO2015112767A2 (en) | 2014-01-22 | 2015-01-22 | Novel reverse transcriptases for use in high temperature nucleic acid synthesis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017503521A JP2017503521A (ja) | 2017-02-02 |
| JP2017503521A5 true JP2017503521A5 (enExample) | 2018-02-01 |
Family
ID=52444678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016548054A Pending JP2017503521A (ja) | 2014-01-22 | 2015-01-22 | 高温核酸合成で使用するための新規な逆転写酵素 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9663770B2 (enExample) |
| EP (1) | EP3097190A2 (enExample) |
| JP (1) | JP2017503521A (enExample) |
| KR (1) | KR20160113177A (enExample) |
| CN (1) | CN106164261A (enExample) |
| WO (1) | WO2015112767A2 (enExample) |
Families Citing this family (73)
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| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| EP3186374A1 (en) * | 2014-08-25 | 2017-07-05 | Life Technologies Corporation | Reversibly inactivated thermostable reverse transcriptases, compositions and methods for use |
| WO2017070632A2 (en) | 2015-10-23 | 2017-04-27 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| EP3516050B1 (en) | 2016-09-23 | 2021-08-11 | New England Biolabs, Inc. | A mutant reverse transcriptase |
| US9580698B1 (en) * | 2016-09-23 | 2017-02-28 | New England Biolabs, Inc. | Mutant reverse transcriptase |
| KR20240007715A (ko) | 2016-10-14 | 2024-01-16 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| US11220677B2 (en) | 2016-12-14 | 2022-01-11 | Takara Bio Inc. | Heat-resistant reverse transcriptase mutant |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| KR20190123328A (ko) | 2017-03-09 | 2019-10-31 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 암 백신 |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| CN110914310A (zh) | 2017-03-10 | 2020-03-24 | 哈佛大学的校长及成员们 | 胞嘧啶至鸟嘌呤碱基编辑器 |
| EP3610006B1 (en) | 2017-04-11 | 2021-05-19 | Roche Diagnostics GmbH | Mutant reverse transcriptase with increased thermal stability as well as products, methods and uses involving the same |
| SG11201908596WA (en) * | 2017-04-26 | 2019-11-28 | 10X Genomics Inc | Mmlv reverse transcriptase variants |
| EP3658573A1 (en) | 2017-07-28 | 2020-06-03 | President and Fellows of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace) |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| EP3820495A4 (en) | 2018-07-09 | 2022-07-20 | The Broad Institute Inc. | RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| WO2020132966A1 (zh) * | 2018-12-26 | 2020-07-02 | 深圳华大生命科学研究院 | 酶活性提高的逆转录酶及其应用 |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020191241A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CN114174503B (zh) * | 2019-07-26 | 2024-06-28 | 东洋纺株式会社 | 稳定性优异的突变型逆转录酶 |
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| CN112795550B (zh) * | 2019-11-13 | 2024-05-31 | 广州达安基因股份有限公司 | 耐高温逆转录酶突变体 |
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| CN112795547B (zh) * | 2019-11-13 | 2024-05-31 | 广州达安基因股份有限公司 | 高逆转录效率的逆转录酶突变体 |
| CN112795551B (zh) * | 2019-11-13 | 2024-01-30 | 广州达安基因股份有限公司 | 一种耐高温逆转录酶突变体及其应用 |
| CN112795546B (zh) * | 2019-11-13 | 2024-02-27 | 广州达安基因股份有限公司 | 一种具有高逆转录效率的耐高温逆转录酶突变体及其应用 |
| EP4461810A3 (en) | 2019-12-11 | 2025-04-09 | 10X Genomics, Inc. | Reverse transcriptase variants |
| WO2021146187A1 (en) | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Emulsion based drug screening |
| JP7730449B2 (ja) | 2020-01-13 | 2025-08-28 | イルミナ インコーポレイテッド | 単一細胞遺伝子プロファイリングのための方法およびシステム |
| JP2023511279A (ja) | 2020-01-13 | 2023-03-17 | フルーエント バイオサイエンシーズ インコーポレイテッド | 単一細胞シーケンシング |
| US20210214721A1 (en) * | 2020-01-13 | 2021-07-15 | Fluent Biosciences Inc. | Reverse transcription during template emulsification |
| US12663345B2 (en) | 2020-01-13 | 2026-06-23 | Illumina, Inc. | Devices for generating monodisperse droplets from a bulk liquid |
| JP2023515499A (ja) * | 2020-02-21 | 2023-04-13 | バイオオー サイエンティフィック コーポレーション | 超並列核酸シーケンシングライブラリを生成するための組成物及び方法 |
| BR112022017715A2 (pt) | 2020-03-04 | 2022-11-16 | Flagship Pioneering Innovations Vi Llc | Métodos e composições para modular um genoma |
| CA3175931A1 (en) | 2020-03-16 | 2021-09-23 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
| US12428685B2 (en) | 2020-03-24 | 2025-09-30 | Illumina, Inc. | Viral detection using template emulsification |
| CA3177481A1 (en) | 2020-05-08 | 2021-11-11 | David R. Liu | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| CN111647576B (zh) * | 2020-06-24 | 2021-02-09 | 南京诺唯赞生物科技股份有限公司 | 一种热稳定性逆转录酶突变体及其应用 |
| KR102338690B1 (ko) * | 2020-07-09 | 2021-12-14 | (주)바이오닉스 | 항체결합단백질 및 우라실 dna 글리코실레이즈 효소를 포함하는 융합 단백질, 및 상기 융합 단백질의 용도 |
| CA3189597A1 (en) | 2020-07-15 | 2022-01-20 | Fluent Biosciences Inc. | Tiered ligation oligos |
| US12559734B2 (en) * | 2020-07-20 | 2026-02-24 | Integrated Dna Technologies, Inc. | Reverse transcriptase mutants with increased activity and thermostability |
| US12435318B2 (en) * | 2020-07-20 | 2025-10-07 | Integrated Dna Technologies, Inc. | Reverse transcriptase mutants with increased activity and thermostability |
| CN114480335B (zh) * | 2020-11-13 | 2024-06-07 | 广州达安基因股份有限公司 | 逆转录酶及逆转录检测试剂 |
| CN114480331A (zh) * | 2020-11-13 | 2022-05-13 | 广州达安基因股份有限公司 | Mmlv酶组合突变体 |
| CN114480330B (zh) * | 2020-11-13 | 2024-11-01 | 广州达安基因股份有限公司 | 多突变位点的逆转录酶突变体 |
| CN114480338B (zh) * | 2020-11-13 | 2024-04-05 | 广州达安基因股份有限公司 | 用于核酸检测的逆转录酶突变体 |
| CN112251423B (zh) * | 2020-12-08 | 2021-03-19 | 北京健为医学检验实验室有限公司 | 一种m-mlv逆转录酶体及其应用 |
| CN113234858A (zh) * | 2021-05-20 | 2021-08-10 | 阿吉安(福州)基因医学检验实验室有限公司 | 一种covid-19的检测试剂盒 |
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| CN113355303B (zh) * | 2021-06-07 | 2023-06-16 | 中元汇吉生物技术股份有限公司 | 一种m-mlv逆转录酶突变体及其应用 |
| KR20260037664A (ko) | 2021-09-08 | 2026-03-18 | 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 | 게놈을 조절하기 위한 방법 및 조성물 |
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| CN117586983B (zh) * | 2022-08-11 | 2026-05-29 | 菲鹏生物股份有限公司 | 一种逆转录酶、核酸分子及合成cDNA的方法 |
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| CN116814581A (zh) * | 2022-10-13 | 2023-09-29 | 苏州拜科生物科技有限公司 | 一种热稳定性反转录酶突变体基因、制备方法和应用 |
| CN120359295A (zh) * | 2022-12-21 | 2025-07-22 | 成对植物服务股份有限公司 | 工程化蛋白质和其使用方法 |
| CN116004568B (zh) * | 2023-02-07 | 2025-10-17 | 北京诺派生物科技有限公司 | 一种可用于核酸检测的耐高温反转录酶及其制备方法与应用 |
| CN116286721B (zh) * | 2023-03-29 | 2026-06-26 | 苏州源启生物科技有限公司 | 一种耐热型逆转录酶突变体及其制备方法和应用 |
| CN116286723A (zh) * | 2023-04-28 | 2023-06-23 | 深圳市艾伟迪生物科技有限公司 | 一种基于蛋白定向进化的快速逆转录酶及其应用 |
| GB2630305A (en) * | 2023-05-22 | 2024-11-27 | Thermo Fisher Scientific Baltics Uab | Reverse transcriptase with improved properties |
| CN119592538A (zh) * | 2023-06-30 | 2025-03-11 | 翌圣生物科技(上海)股份有限公司 | 一种鼠白血病病毒逆转录酶突变体及其应用 |
| WO2026003215A1 (fr) | 2024-06-27 | 2026-01-02 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Bases modifiées chimiquement avec un groupement clivable et méthodes de sélection d'acides nucléiques fonctionnels les comprenant |
| WO2026135182A1 (ko) * | 2024-12-19 | 2026-06-25 | 주식회사 씨젠 | 돌연변이 m-mlv 역전사 효소 |
| CN121204003B (zh) * | 2025-10-17 | 2026-07-17 | 珠海宝锐生物科技有限公司 | 一种mmlv酶突变体、制备方法及其应用 |
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|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| JPH0779694B2 (ja) | 1985-07-09 | 1995-08-30 | カドラント バイオリソ−シズ リミテツド | 蛋白質および同類品の保護 |
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-
2015
- 2015-01-22 US US14/603,256 patent/US9663770B2/en active Active
- 2015-01-22 EP EP15702365.6A patent/EP3097190A2/en not_active Withdrawn
- 2015-01-22 JP JP2016548054A patent/JP2017503521A/ja active Pending
- 2015-01-22 CN CN201580014630.7A patent/CN106164261A/zh active Pending
- 2015-01-22 KR KR1020167022831A patent/KR20160113177A/ko not_active Withdrawn
- 2015-01-22 WO PCT/US2015/012534 patent/WO2015112767A2/en not_active Ceased
-
2017
- 2017-05-26 US US15/607,247 patent/US20180010105A1/en not_active Abandoned
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