JP2017503521A - 高温核酸合成で使用するための新規な逆転写酵素 - Google Patents

高温核酸合成で使用するための新規な逆転写酵素 Download PDF

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JP2017503521A
JP2017503521A JP2016548054A JP2016548054A JP2017503521A JP 2017503521 A JP2017503521 A JP 2017503521A JP 2016548054 A JP2016548054 A JP 2016548054A JP 2016548054 A JP2016548054 A JP 2016548054A JP 2017503521 A JP2017503521 A JP 2017503521A
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reverse transcriptase
mutant
mlv
nucleic acid
activity
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JP2017503521A5 (enExample
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ジェフリー ロジャーズ
ジェフリー ロジャーズ
ジェイソン ポッター
ジェイソン ポッター
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ライフ テクノロジーズ コーポレーション
ライフ テクノロジーズ コーポレーション
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Analytical Chemistry (AREA)
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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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JP2016548054A 2014-01-22 2015-01-22 高温核酸合成で使用するための新規な逆転写酵素 Pending JP2017503521A (ja)

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US201461930395P 2014-01-22 2014-01-22
US61/930,395 2014-01-22
PCT/US2015/012534 WO2015112767A2 (en) 2014-01-22 2015-01-22 Novel reverse transcriptases for use in high temperature nucleic acid synthesis

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EP (1) EP3097190A2 (enExample)
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KR (1) KR20160113177A (enExample)
CN (1) CN106164261A (enExample)
WO (1) WO2015112767A2 (enExample)

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KR102338690B1 (ko) * 2020-07-09 2021-12-14 (주)바이오닉스 항체결합단백질 및 우라실 dna 글리코실레이즈 효소를 포함하는 융합 단백질, 및 상기 융합 단백질의 용도
JP2023515499A (ja) * 2020-02-21 2023-04-13 バイオオー サイエンティフィック コーポレーション 超並列核酸シーケンシングライブラリを生成するための組成物及び方法
JP2023517187A (ja) * 2020-03-04 2023-04-24 フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー ゲノムを調節するための方法及び組成物

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US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
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JP2011516072A (ja) * 2008-04-10 2011-05-26 フェルメンタス ユーエービー 核酸の生成

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JP2023515499A (ja) * 2020-02-21 2023-04-13 バイオオー サイエンティフィック コーポレーション 超並列核酸シーケンシングライブラリを生成するための組成物及び方法
JP2023517187A (ja) * 2020-03-04 2023-04-24 フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー ゲノムを調節するための方法及び組成物
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KR102338690B1 (ko) * 2020-07-09 2021-12-14 (주)바이오닉스 항체결합단백질 및 우라실 dna 글리코실레이즈 효소를 포함하는 융합 단백질, 및 상기 융합 단백질의 용도

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