CN106164261A - 适用于高温核酸合成的新颖逆转录酶 - Google Patents

适用于高温核酸合成的新颖逆转录酶 Download PDF

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Publication number
CN106164261A
CN106164261A CN201580014630.7A CN201580014630A CN106164261A CN 106164261 A CN106164261 A CN 106164261A CN 201580014630 A CN201580014630 A CN 201580014630A CN 106164261 A CN106164261 A CN 106164261A
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Prior art keywords
reverse transcriptase
reverse
mlv
transcriptases
nucleic acid
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CN201580014630.7A
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Chinese (zh)
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J·罗格斯
J·波特
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Life Technologies Inc
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Life Technologies Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201580014630.7A 2014-01-22 2015-01-22 适用于高温核酸合成的新颖逆转录酶 Pending CN106164261A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461930395P 2014-01-22 2014-01-22
US61/930,395 2014-01-22
PCT/US2015/012534 WO2015112767A2 (en) 2014-01-22 2015-01-22 Novel reverse transcriptases for use in high temperature nucleic acid synthesis

Publications (1)

Publication Number Publication Date
CN106164261A true CN106164261A (zh) 2016-11-23

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Family Applications (1)

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CN201580014630.7A Pending CN106164261A (zh) 2014-01-22 2015-01-22 适用于高温核酸合成的新颖逆转录酶

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US (2) US9663770B2 (enExample)
EP (1) EP3097190A2 (enExample)
JP (1) JP2017503521A (enExample)
KR (1) KR20160113177A (enExample)
CN (1) CN106164261A (enExample)
WO (1) WO2015112767A2 (enExample)

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CN110637084A (zh) * 2017-04-26 2019-12-31 10X基因组学有限公司 Mmlv逆转录酶变体
CN110709513A (zh) * 2017-04-11 2020-01-17 豪夫迈·罗氏有限公司 具有热稳定性增加的突变型逆转录酶以及涉及其的产物、方法和用途
WO2020132966A1 (zh) * 2018-12-26 2020-07-02 深圳华大生命科学研究院 酶活性提高的逆转录酶及其应用
CN111647576A (zh) * 2020-06-24 2020-09-11 南京诺唯赞生物科技股份有限公司 一种热稳定性逆转录酶突变体及其应用
CN112795549A (zh) * 2019-11-13 2021-05-14 中山大学达安基因股份有限公司 一种逆转录酶突变体
CN112795547A (zh) * 2019-11-13 2021-05-14 中山大学达安基因股份有限公司 高逆转录效率的逆转录酶突变体
CN112795550A (zh) * 2019-11-13 2021-05-14 中山大学达安基因股份有限公司 耐高温逆转录酶突变体
CN112795546A (zh) * 2019-11-13 2021-05-14 中山大学达安基因股份有限公司 一种具有高逆转录效率的耐高温逆转录酶突变体及其应用
WO2021093845A1 (zh) * 2019-11-13 2021-05-20 中山大学达安基因股份有限公司 耐高温逆转录酶突变体及其应用
CN113234858A (zh) * 2021-05-20 2021-08-10 阿吉安(福州)基因医学检验实验室有限公司 一种covid-19的检测试剂盒
CN113355303A (zh) * 2021-06-07 2021-09-07 重庆中元汇吉生物技术有限公司 一种m-mlv逆转录酶突变体及其应用
CN114174503A (zh) * 2019-07-26 2022-03-11 东洋纺株式会社 稳定性优异的突变型逆转录酶
CN114480338A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 用于核酸检测的逆转录酶突变体
CN114480330A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 多突变位点的逆转录酶突变体
CN114480331A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 Mmlv酶组合突变体
CN114480335A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 逆转录酶及逆转录检测试剂
WO2024032512A1 (zh) * 2022-08-11 2024-02-15 菲鹏生物股份有限公司 一种逆转录酶、核酸分子及合成cDNA的方法

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US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
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