JP2016518833A5 - - Google Patents
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- JP2016518833A5 JP2016518833A5 JP2016509127A JP2016509127A JP2016518833A5 JP 2016518833 A5 JP2016518833 A5 JP 2016518833A5 JP 2016509127 A JP2016509127 A JP 2016509127A JP 2016509127 A JP2016509127 A JP 2016509127A JP 2016518833 A5 JP2016518833 A5 JP 2016518833A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- isomerase
- extract
- exogenous
- interest
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108090000623 proteins and genes Proteins 0.000 claims description 80
- 239000000284 extract Substances 0.000 claims description 73
- 102000004169 proteins and genes Human genes 0.000 claims description 63
- 102000006010 Protein Disulfide-Isomerase Human genes 0.000 claims description 62
- 108020003519 protein disulfide isomerase Proteins 0.000 claims description 62
- 238000000034 method Methods 0.000 claims description 57
- 241000894006 Bacteria Species 0.000 claims description 44
- 230000001580 bacterial effect Effects 0.000 claims description 39
- 101000878213 Homo sapiens Inactive peptidyl-prolyl cis-trans isomerase FKBP6 Proteins 0.000 claims description 29
- 102100036984 Inactive peptidyl-prolyl cis-trans isomerase FKBP6 Human genes 0.000 claims description 29
- 108010020062 Peptidylprolyl Isomerase Proteins 0.000 claims description 22
- 102000009658 Peptidylprolyl Isomerase Human genes 0.000 claims description 22
- 230000015572 biosynthetic process Effects 0.000 claims description 18
- 238000003786 synthesis reaction Methods 0.000 claims description 18
- 241000588724 Escherichia coli Species 0.000 claims description 15
- 230000014509 gene expression Effects 0.000 claims description 15
- 150000001413 amino acids Chemical class 0.000 claims description 12
- 238000011534 incubation Methods 0.000 claims description 12
- 238000001243 protein synthesis Methods 0.000 claims description 12
- 210000003705 ribosome Anatomy 0.000 claims description 12
- 230000014616 translation Effects 0.000 claims description 12
- 102000039446 nucleic acids Human genes 0.000 claims description 11
- 108020004707 nucleic acids Proteins 0.000 claims description 11
- 150000007523 nucleic acids Chemical class 0.000 claims description 11
- 230000010627 oxidative phosphorylation Effects 0.000 claims description 10
- 102000004195 Isomerases Human genes 0.000 claims description 9
- 108090000769 Isomerases Proteins 0.000 claims description 9
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 claims description 7
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 6
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 6
- 230000000975 bioactive effect Effects 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 2
- 230000002045 lasting effect Effects 0.000 claims description 2
- 102000035195 Peptidases Human genes 0.000 claims 4
- 108091005804 Peptidases Proteins 0.000 claims 4
- 235000019833 protease Nutrition 0.000 claims 4
- 101001065501 Escherichia phage MS2 Lysis protein Proteins 0.000 claims 1
- 230000008827 biological function Effects 0.000 claims 1
- 125000001151 peptidyl group Chemical group 0.000 claims 1
- 108090000175 Cis-trans-isomerases Proteins 0.000 description 1
- 102000003813 Cis-trans-isomerases Human genes 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361813914P | 2013-04-19 | 2013-04-19 | |
| US61/813,914 | 2013-04-19 | ||
| US201461937069P | 2014-02-07 | 2014-02-07 | |
| US61/937,069 | 2014-02-07 | ||
| PCT/US2014/034643 WO2014172631A2 (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018136909A Division JP2018157837A (ja) | 2013-04-19 | 2018-07-20 | 上昇したレベルの外因性シャペロンを有する細胞抽出物を用いる細菌無細胞合成系における生物活性のあるタンパク質の発現 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016518833A JP2016518833A (ja) | 2016-06-30 |
| JP2016518833A5 true JP2016518833A5 (enExample) | 2017-07-20 |
| JP6463732B2 JP6463732B2 (ja) | 2019-02-06 |
Family
ID=50733442
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016509127A Active JP6463732B2 (ja) | 2013-04-19 | 2014-04-18 | 上昇したレベルの外因性シャペロンを有する細胞抽出物を用いる細菌無細胞合成系における生物活性のあるタンパク質の発現 |
| JP2018136909A Withdrawn JP2018157837A (ja) | 2013-04-19 | 2018-07-20 | 上昇したレベルの外因性シャペロンを有する細胞抽出物を用いる細菌無細胞合成系における生物活性のあるタンパク質の発現 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018136909A Withdrawn JP2018157837A (ja) | 2013-04-19 | 2018-07-20 | 上昇したレベルの外因性シャペロンを有する細胞抽出物を用いる細菌無細胞合成系における生物活性のあるタンパク質の発現 |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US10190145B2 (enExample) |
| EP (1) | EP2986731B1 (enExample) |
| JP (2) | JP6463732B2 (enExample) |
| KR (1) | KR102289258B1 (enExample) |
| CN (1) | CN105283556B (enExample) |
| AU (1) | AU2014253785B2 (enExample) |
| CA (1) | CA2909516C (enExample) |
| DK (1) | DK2986731T3 (enExample) |
| ES (1) | ES2922358T3 (enExample) |
| HU (1) | HUE059565T2 (enExample) |
| IL (1) | IL242148B (enExample) |
| SG (2) | SG10202108497TA (enExample) |
| WO (1) | WO2014172631A2 (enExample) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HUE059565T2 (hu) * | 2013-04-19 | 2022-11-28 | Sutro Biopharma Inc | Biológiailag aktív fehérjék expressziója bakteriális sejtmentes szintézisrendszerben, emelkedett exogén chaperonszintû sejtextraktumok segítségével |
| US10316322B2 (en) * | 2014-07-02 | 2019-06-11 | Sutro Biopharma, Inc. | High growth capacity auxotrophic Escherichia coli and methods of use |
| WO2016073794A1 (en) | 2014-11-05 | 2016-05-12 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
| WO2016073791A1 (en) | 2014-11-05 | 2016-05-12 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
| WO2016100889A1 (en) * | 2014-12-19 | 2016-06-23 | Sutro Biopharma, Inc. | Codon optimization for titer and fidelity improvement |
| KR102489471B1 (ko) | 2014-12-30 | 2023-01-18 | 셀진 코포레이션 | 항-cd47 항체 및 그 용도 |
| SI3265557T1 (sl) * | 2015-03-06 | 2020-02-28 | F. Hoffmann-La Roche Ag | Ultraprečiščena DsbA in DsbC ter postopki njune izdelave in uporabe |
| US20170029798A1 (en) * | 2015-07-27 | 2017-02-02 | Cellivery Therapeutics, Inc. | Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD) |
| MX2018002068A (es) * | 2015-08-20 | 2018-06-06 | Genentech Inc | Purificacion de fkpa y usos de la misma para producir polipeptidos recombinantes. |
| CN105198983B (zh) * | 2015-09-11 | 2018-11-06 | 中国石油大学(华东) | 一种利于七次跨膜蛋白ccr5功能性表达的方法 |
| CN106190940B (zh) * | 2016-07-19 | 2019-06-14 | 北京三元基因药业股份有限公司 | 表达抗TNF抗体Fab片段的重组大肠杆菌工程菌 |
| CN106399352B (zh) * | 2016-11-09 | 2020-04-17 | 华东理工大学 | 调节目标蛋白表达的折叠因子及其应用 |
| DK3562940T3 (da) * | 2016-12-30 | 2022-07-11 | Natures Toolbox Inc | Cellefrit ekspressionssystem med hidtil ukendt, uorganisk polyphosphatbaseret energiregeneration |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| KR20240110660A (ko) | 2016-12-30 | 2024-07-15 | 박사이트, 인코포레이티드 | 비자연 아미노산을 갖는 폴리펩타이드-항원 접합체 |
| US12227778B2 (en) | 2016-12-30 | 2025-02-18 | Nature's Toolbox, Inc. | Cell-free expression system having novel inorganic polyphosphate-based energy regeneration |
| AR112756A1 (es) | 2017-07-11 | 2019-12-11 | Synthorx Inc | Incorporación de nucleótidos no naturales, y su método |
| MX2020001336A (es) | 2017-08-03 | 2020-08-20 | Synthorx Inc | Conjugados de citoquina para el tratamiento de enfermedades autoinmunes. |
| US20200181670A1 (en) * | 2017-08-11 | 2020-06-11 | Synvitrobio, Inc. | Improved In Vitro Transcription/Translation (TXTL) System and Use Thereof |
| WO2019127469A1 (zh) * | 2017-12-29 | 2019-07-04 | 康码（上海）生物科技有限公司 | 一种高效的能源再生体系(bes)、试剂盒及其制备方法 |
| TWI834636B (zh) | 2018-02-26 | 2024-03-11 | 美商欣爍克斯公司 | Il-15結合物及其用途 |
| WO2020014543A2 (en) * | 2018-07-11 | 2020-01-16 | Actym Therapeutics, Inc. | Engineered immunostimulatory bacterial strains and uses thereof |
| WO2020096959A1 (en) * | 2018-11-05 | 2020-05-14 | Genentech, Inc. | Methods of producing two chain proteins in prokaryotic host cells |
| JP7426389B2 (ja) * | 2018-11-08 | 2024-02-01 | ストロ バイオファーマ， インコーポレイテッド | 酸化的細胞質を有する大腸菌株 |
| JP7636323B2 (ja) | 2018-11-08 | 2025-02-26 | シンソークス， インコーポレイテッド | インターロイキン１０コンジュゲートおよびその使用 |
| CN109371049A (zh) * | 2018-11-14 | 2019-02-22 | 天津大学 | 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用 |
| JP7702872B2 (ja) | 2019-02-06 | 2025-07-04 | シンソークス， インコーポレイテッド | Ｉｌ－２コンジュゲートおよびその使用方法 |
| US12024709B2 (en) | 2019-02-27 | 2024-07-02 | Actym Therapeutics, Inc. | Immunostimulatory bacteria engineered to colonize tumors, tumor-resident immune cells, and the tumor microenvironment |
| CA3150163A1 (en) | 2019-08-15 | 2021-02-18 | Synthorx, Inc. | Immuno oncology combination therapies with il-2 conjugates |
| TW202124385A (zh) | 2019-09-10 | 2021-07-01 | 美商欣爍克斯公司 | 治療自體免疫疾病之il-2接合物及使用方法 |
| MX2022005251A (es) | 2019-11-04 | 2022-06-08 | Synthorx Inc | Conjugados de interleuquina 10 y sus usos. |
| EP4056584A4 (en) * | 2019-11-08 | 2024-06-05 | Korea Institute of Ocean Science & Technology | NOVEL HUMAN EPIDERMAL GROWTH FACTOR-TF FUSION PROTEIN AND USE THEREOF |
| EP4114931A4 (en) * | 2020-03-05 | 2024-05-01 | Curie Co. Inc. | METHODS FOR CELL PROTEIN EXPRESSION OF MATURE POLYPEPTIDES DERIVED FROM ZYMOGENS AND PROPROTEINS |
| CA3175683A1 (en) * | 2020-04-29 | 2021-11-04 | Xueyang FENG | Compositions and methods for enhancing recombinant biosynthesis of cannabinoids |
| BR112022026236A2 (pt) | 2020-06-25 | 2023-01-17 | Synthorx Inc | Terapia de combinação de imuno-oncologia com conjugados de il-2 e anticorpos anti-egfr |
| KR20220000028U (ko) | 2020-06-29 | 2022-01-05 | 채인자 | 농작물용 농약 간편 분무장치 |
| US20220089690A1 (en) * | 2020-09-14 | 2022-03-24 | Sutro Biopharma, Inc. | Method for large scale production of antibodies using a cell-free protein synthesis system |
| IL301612A (en) | 2020-10-09 | 2023-05-01 | Synthorx Inc | Immuno oncology therapies with il-2 conjugates |
| BR112023006364A2 (pt) | 2020-10-09 | 2023-05-09 | Synthorx Inc | Terapia de combinação de imuno-oncologia com conjugados de il-2 e pembrolizumabe |
| WO2022174101A1 (en) | 2021-02-12 | 2022-08-18 | Synthorx, Inc. | Skin cancer combination therapy with il-2 conjugates and cemiplimab |
| EP4291243A1 (en) | 2021-02-12 | 2023-12-20 | Synthorx, Inc. | Lung cancer combination therapy with il-2 conjugates and an anti-pd-1 antibody or antigen-binding fragment thereof |
| EP4346903A1 (en) | 2021-06-03 | 2024-04-10 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| EP4452327A1 (en) | 2021-12-20 | 2024-10-30 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122750A1 (en) | 2021-12-23 | 2023-06-29 | Synthorx, Inc. | Cancer combination therapy with il-2 conjugates and cetuximab |
| CA3255881A1 (en) * | 2022-06-17 | 2023-12-21 | Insitro Inc | In situ sequencing of RNA transcripts with non-uniform 5' ends |
| CN115028691B (zh) * | 2022-06-29 | 2024-09-10 | 青岛硕景生物科技有限公司 | 一种检测试纸用的独立质控系统、杂交瘤细胞株、质控线用包被单克隆抗体及其应用 |
| WO2024136899A1 (en) | 2022-12-21 | 2024-06-27 | Synthorx, Inc. | Cancer therapy with il-2 conjugates and chimeric antigen receptor therapies |
| WO2024196937A1 (en) | 2023-03-20 | 2024-09-26 | Synthorx, Inc. | Cancer therapy with il-2 peg conjugates |
| CN116925196B (zh) * | 2023-06-08 | 2025-04-18 | 齐鲁工业大学(山东省科学院) | 小分子热休克蛋白rsp_1572在提高宿主菌环境耐受性中的应用 |
| WO2025158385A1 (en) | 2024-01-25 | 2025-07-31 | Genzyme Corporation | Pegylated il-2 for suppressing adaptive immune response to gene therapy |
| CN118048325B (zh) * | 2024-04-16 | 2024-07-16 | 天津凯莱英生物科技有限公司 | 酶的高通量筛选方法及酶库的构建方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10145694A1 (de) | 2001-09-17 | 2003-04-03 | Roche Diagnostics Gmbh | Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung |
| WO2004081033A2 (en) * | 2003-03-11 | 2004-09-23 | National Institutes Of Health | Synthesis of proteins by cell-free protein expression |
| JP2005253432A (ja) | 2004-03-15 | 2005-09-22 | Sekisui Chem Co Ltd | タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット |
| JP4561243B2 (ja) | 2004-08-27 | 2010-10-13 | 株式会社豊田中央研究所 | 無細胞タンパク質合成系のための媒体 |
| AU2007325952B8 (en) * | 2006-06-29 | 2013-06-27 | The Board Of Trustees Of The Leland Stanford Junior University | Cell-free synthesis of proteins containing unnatural amino acids |
| CA2673765A1 (en) * | 2007-01-18 | 2008-07-24 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced cell-free synthesis of active proteins containing disulfide bonds |
| CN102348807B (zh) | 2009-01-12 | 2015-04-15 | 苏特罗生物制药公司 | 利用体外蛋白合成体系将非天然氨基酸引入蛋白的单装载体系 |
| CN102732548A (zh) | 2012-05-16 | 2012-10-17 | 中国农业大学 | 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用 |
| JP6421122B2 (ja) * | 2012-10-12 | 2018-11-07 | ストロ バイオファーマ， インコーポレイテッド | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
| HUE059565T2 (hu) | 2013-04-19 | 2022-11-28 | Sutro Biopharma Inc | Biológiailag aktív fehérjék expressziója bakteriális sejtmentes szintézisrendszerben, emelkedett exogén chaperonszintû sejtextraktumok segítségével |
-
2014
- 2014-04-18 HU HUE14724974A patent/HUE059565T2/hu unknown
- 2014-04-18 SG SG10202108497TA patent/SG10202108497TA/en unknown
- 2014-04-18 CA CA2909516A patent/CA2909516C/en active Active
- 2014-04-18 DK DK14724974.2T patent/DK2986731T3/da active
- 2014-04-18 KR KR1020157032515A patent/KR102289258B1/ko active Active
- 2014-04-18 JP JP2016509127A patent/JP6463732B2/ja active Active
- 2014-04-18 ES ES14724974T patent/ES2922358T3/es active Active
- 2014-04-18 AU AU2014253785A patent/AU2014253785B2/en active Active
- 2014-04-18 US US14/256,324 patent/US10190145B2/en active Active
- 2014-04-18 CN CN201480032258.8A patent/CN105283556B/zh active Active
- 2014-04-18 WO PCT/US2014/034643 patent/WO2014172631A2/en not_active Ceased
- 2014-04-18 EP EP14724974.2A patent/EP2986731B1/en active Active
- 2014-04-18 SG SG11201508642WA patent/SG11201508642WA/en unknown
-
2015
- 2015-10-18 IL IL242148A patent/IL242148B/en active IP Right Grant
-
2018
- 2018-07-20 JP JP2018136909A patent/JP2018157837A/ja not_active Withdrawn
- 2018-12-03 US US16/208,245 patent/US10774354B2/en active Active
-
2020
- 2020-08-12 US US16/991,607 patent/US20210054429A1/en active Pending
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