SG11201508642WA - Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones - Google Patents
Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperonesInfo
- Publication number
- SG11201508642WA SG11201508642WA SG11201508642WA SG11201508642WA SG11201508642WA SG 11201508642W A SG11201508642W A SG 11201508642WA SG 11201508642W A SG11201508642W A SG 11201508642WA SG 11201508642W A SG11201508642W A SG 11201508642WA SG 11201508642W A SG11201508642W A SG 11201508642WA
- Authority
- SG
- Singapore
- Prior art keywords
- expression
- biologically active
- elevated levels
- synthesis system
- active proteins
- Prior art date
Links
- 102000005431 Molecular Chaperones Human genes 0.000 title 1
- 108010006519 Molecular Chaperones Proteins 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 title 1
- 230000015572 biosynthetic process Effects 0.000 title 1
- 239000000284 extract Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361813914P | 2013-04-19 | 2013-04-19 | |
| US201461937069P | 2014-02-07 | 2014-02-07 | |
| PCT/US2014/034643 WO2014172631A2 (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201508642WA true SG11201508642WA (en) | 2015-11-27 |
Family
ID=50733442
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201508642WA SG11201508642WA (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
| SG10202108497TA SG10202108497TA (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10202108497TA SG10202108497TA (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US10190145B2 (enExample) |
| EP (1) | EP2986731B1 (enExample) |
| JP (2) | JP6463732B2 (enExample) |
| KR (1) | KR102289258B1 (enExample) |
| CN (1) | CN105283556B (enExample) |
| AU (1) | AU2014253785B2 (enExample) |
| CA (1) | CA2909516C (enExample) |
| DK (1) | DK2986731T3 (enExample) |
| ES (1) | ES2922358T3 (enExample) |
| HU (1) | HUE059565T2 (enExample) |
| IL (1) | IL242148B (enExample) |
| SG (2) | SG11201508642WA (enExample) |
| WO (1) | WO2014172631A2 (enExample) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2014172631A2 (en) | 2013-04-19 | 2014-10-23 | Sutro Biopharma, Inc. | Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression |
| EP3164501A4 (en) * | 2014-07-02 | 2018-01-17 | Sutro Biopharma, Inc. | High growth capacity auxotrophic escherichia coli and methods of use |
| AU2015342964B2 (en) * | 2014-11-05 | 2021-06-24 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
| BR112017009152A2 (pt) | 2014-11-05 | 2018-03-06 | Genentech Inc | métodos de produção de proteínas de duas cadeias em bactérias |
| WO2016100889A1 (en) * | 2014-12-19 | 2016-06-23 | Sutro Biopharma, Inc. | Codon optimization for titer and fidelity improvement |
| HK1245154A1 (zh) * | 2014-12-30 | 2018-08-24 | 细胞基因公司 | 抗cd47抗体及其用途 |
| CA2978256A1 (en) * | 2015-03-06 | 2016-09-15 | Genentech, Inc. | Ultrapurified dsba and dsbc and methods of making and using the same |
| US20170029798A1 (en) * | 2015-07-27 | 2017-02-02 | Cellivery Therapeutics, Inc. | Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD) |
| HK1259368A1 (zh) * | 2015-08-20 | 2019-11-29 | F. Hoffmann-La Roche Ag | Fkpa的纯化及其用於生产重组多肽的用途 |
| CN105198983B (zh) * | 2015-09-11 | 2018-11-06 | 中国石油大学(华东) | 一种利于七次跨膜蛋白ccr5功能性表达的方法 |
| CN106190940B (zh) * | 2016-07-19 | 2019-06-14 | 北京三元基因药业股份有限公司 | 表达抗TNF抗体Fab片段的重组大肠杆菌工程菌 |
| CN106399352B (zh) * | 2016-11-09 | 2020-04-17 | 华东理工大学 | 调节目标蛋白表达的折叠因子及其应用 |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| AU2018205001B2 (en) * | 2016-12-30 | 2020-02-20 | Ntxbio, Llc | Cell-free expression system having novel inorganic polyphosphate-based energy regeneration |
| US11998599B2 (en) | 2016-12-30 | 2024-06-04 | Vaxcyte, Inc. | Polypeptide-antigen conjugates with non-natural amino acids |
| US12227778B2 (en) | 2016-12-30 | 2025-02-18 | Nature's Toolbox, Inc. | Cell-free expression system having novel inorganic polyphosphate-based energy regeneration |
| CN111051512A (zh) | 2017-07-11 | 2020-04-21 | 辛索克斯公司 | 非天然核苷酸的掺入及其方法 |
| SG11202000939PA (en) | 2017-08-03 | 2020-02-27 | Synthorx Inc | Cytokine conjugates for the treatment of proliferative and infectious diseases |
| US20200181670A1 (en) * | 2017-08-11 | 2020-06-11 | Synvitrobio, Inc. | Improved In Vitro Transcription/Translation (TXTL) System and Use Thereof |
| WO2019127469A1 (zh) * | 2017-12-29 | 2019-07-04 | 康码（上海）生物科技有限公司 | 一种高效的能源再生体系(bes)、试剂盒及其制备方法 |
| AR114445A1 (es) | 2018-02-26 | 2020-09-09 | Synthorx Inc | Conjugados de il-15, y sus usos |
| JP7340591B2 (ja) * | 2018-07-11 | 2023-09-07 | アクティム・セラピューティクス・インコーポレイテッド | 遺伝子操作された免疫刺激性細菌菌株およびその使用 |
| AU2019375413A1 (en) * | 2018-11-05 | 2021-05-27 | Genentech, Inc. | Methods of producing two chain proteins in prokaryotic host cells |
| AU2019376076B9 (en) | 2018-11-08 | 2025-06-19 | Synthorx, Inc. | Interleukin 10 conjugates and uses thereof |
| WO2020097385A1 (en) * | 2018-11-08 | 2020-05-14 | Sutro Biopharma, Inc. | E coli strains having an oxidative cytoplasm |
| CN109371049A (zh) * | 2018-11-14 | 2019-02-22 | 天津大学 | 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用 |
| AU2020218203A1 (en) | 2019-02-06 | 2021-08-26 | Synthorx, Inc. | IL-2 conjugates and methods of use thereof |
| US12024709B2 (en) | 2019-02-27 | 2024-07-02 | Actym Therapeutics, Inc. | Immunostimulatory bacteria engineered to colonize tumors, tumor-resident immune cells, and the tumor microenvironment |
| JP7679355B2 (ja) | 2019-08-15 | 2025-05-19 | シンソークス， インコーポレイテッド | Ｉｌ－２コンジュゲートを用いる免疫腫瘍学併用療 |
| TW202124385A (zh) | 2019-09-10 | 2021-07-01 | 美商欣爍克斯公司 | 治療自體免疫疾病之il-2接合物及使用方法 |
| CA3156405A1 (en) | 2019-11-04 | 2021-05-14 | Synthorx, Inc. | Interleukin 10 conjugates and uses thereof |
| WO2021091304A1 (ko) * | 2019-11-08 | 2021-05-14 | 한국해양과학기술원 | 신규한 인간 상피세포성장인자-tf 융합 단백질 및 이의 용도 |
| EP4114931A4 (en) * | 2020-03-05 | 2024-05-01 | Curie Co. Inc. | METHODS FOR CELL PROTEIN EXPRESSION OF MATURE POLYPEPTIDES DERIVED FROM ZYMOGENS AND PROPROTEINS |
| WO2021222288A1 (en) * | 2020-04-29 | 2021-11-04 | Willow Biosciences, Inc. | Compositions and methods for enhancing recombinant biosynthesis of cannabinoids |
| KR20230027235A (ko) | 2020-06-25 | 2023-02-27 | 신톡스, 인크. | Il-2 콘쥬게이트 및 항-egfr 항체를 사용한 면역 종양학 병용 요법 |
| KR20220000028U (ko) | 2020-06-29 | 2022-01-05 | 채인자 | 농작물용 농약 간편 분무장치 |
| AU2021342284A1 (en) * | 2020-09-14 | 2023-04-20 | Sutro Biopharma, Inc. | Method for large scale production of antibodies using a cell-free protein synthesis system |
| CA3194880A1 (en) | 2020-10-09 | 2022-04-14 | Carolina E. CAFFARO | Immuno oncology therapies with il-2 conjugates |
| JP2023546009A (ja) | 2020-10-09 | 2023-11-01 | シンソークス， インコーポレイテッド | Ｉｌ－２コンジュゲートおよびペムブロリズマブを用いる免疫腫瘍学併用療法 |
| TW202245843A (zh) | 2021-02-12 | 2022-12-01 | 美商欣爍克斯公司 | Ｉｌ－２接合物及西米普利單抗（ｃｅｍｉｐｌｉｍａｂ）之皮膚癌組合療法 |
| EP4291243A1 (en) | 2021-02-12 | 2023-12-20 | Synthorx, Inc. | Lung cancer combination therapy with il-2 conjugates and an anti-pd-1 antibody or antigen-binding fragment thereof |
| TW202313679A (zh) | 2021-06-03 | 2023-04-01 | 美商欣爍克斯公司 | 包含il-2接合物及pd-1拮抗劑之頭頸癌組合療法 |
| WO2023122573A1 (en) | 2021-12-20 | 2023-06-29 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122750A1 (en) | 2021-12-23 | 2023-06-29 | Synthorx, Inc. | Cancer combination therapy with il-2 conjugates and cetuximab |
| AU2023295552A1 (en) * | 2022-06-17 | 2024-12-12 | Insitro, Inc. | In situ sequencing of rna transcripts with non-uniform 5' ends |
| CN115028691B (zh) * | 2022-06-29 | 2024-09-10 | 青岛硕景生物科技有限公司 | 一种检测试纸用的独立质控系统、杂交瘤细胞株、质控线用包被单克隆抗体及其应用 |
| WO2024136899A1 (en) | 2022-12-21 | 2024-06-27 | Synthorx, Inc. | Cancer therapy with il-2 conjugates and chimeric antigen receptor therapies |
| TW202444349A (zh) | 2023-03-20 | 2024-11-16 | 美商欣爍克斯公司 | 使用ｉｌ－２綴合物之癌症療法 |
| CN116925196B (zh) * | 2023-06-08 | 2025-04-18 | 齐鲁工业大学(山东省科学院) | 小分子热休克蛋白rsp_1572在提高宿主菌环境耐受性中的应用 |
| WO2025158385A1 (en) | 2024-01-25 | 2025-07-31 | Genzyme Corporation | Pegylated il-2 for suppressing adaptive immune response to gene therapy |
| CN118048325B (zh) * | 2024-04-16 | 2024-07-16 | 天津凯莱英生物科技有限公司 | 酶的高通量筛选方法及酶库的构建方法 |
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| DE10145694A1 (de) | 2001-09-17 | 2003-04-03 | Roche Diagnostics Gmbh | Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung |
| US20060134739A1 (en) | 2003-03-11 | 2006-06-22 | Chatterjee Deb K | Synthesis of proteins by cell-free protein expression |
| JP2005253432A (ja) * | 2004-03-15 | 2005-09-22 | Sekisui Chem Co Ltd | タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット |
| JP4561243B2 (ja) * | 2004-08-27 | 2010-10-13 | 株式会社豊田中央研究所 | 無細胞タンパク質合成系のための媒体 |
| CN101479379B (zh) * | 2006-06-29 | 2012-01-18 | 利兰·斯坦福青年大学托管委员会 | 含有非天然氨基酸的蛋白质的无细胞合成 |
| EP2109682A4 (en) * | 2007-01-18 | 2012-05-09 | Univ Leland Stanford Junior | Enhanced cell-free synthesis of active proteins with disulfide bonds |
| US20100184134A1 (en) | 2009-01-12 | 2010-07-22 | Sutro Biopharma, Inc. | Dual charging system for selectively introducing non-native amino acids into proteins using an in vitro synthesis method |
| CN102732548A (zh) | 2012-05-16 | 2012-10-17 | 中国农业大学 | 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用 |
| KR102018863B1 (ko) * | 2012-10-12 | 2019-09-05 | 서트로 바이오파마, 인크. | 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화 |
| WO2014172631A2 (en) * | 2013-04-19 | 2014-10-23 | Sutro Biopharma, Inc. | Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression |
-
2014
- 2014-04-18 WO PCT/US2014/034643 patent/WO2014172631A2/en not_active Ceased
- 2014-04-18 HU HUE14724974A patent/HUE059565T2/hu unknown
- 2014-04-18 ES ES14724974T patent/ES2922358T3/es active Active
- 2014-04-18 SG SG11201508642WA patent/SG11201508642WA/en unknown
- 2014-04-18 DK DK14724974.2T patent/DK2986731T3/da active
- 2014-04-18 EP EP14724974.2A patent/EP2986731B1/en active Active
- 2014-04-18 JP JP2016509127A patent/JP6463732B2/ja active Active
- 2014-04-18 AU AU2014253785A patent/AU2014253785B2/en active Active
- 2014-04-18 SG SG10202108497TA patent/SG10202108497TA/en unknown
- 2014-04-18 US US14/256,324 patent/US10190145B2/en active Active
- 2014-04-18 CA CA2909516A patent/CA2909516C/en active Active
- 2014-04-18 CN CN201480032258.8A patent/CN105283556B/zh active Active
- 2014-04-18 KR KR1020157032515A patent/KR102289258B1/ko active Active
-
2015
- 2015-10-18 IL IL242148A patent/IL242148B/en active IP Right Grant
-
2018
- 2018-07-20 JP JP2018136909A patent/JP2018157837A/ja not_active Withdrawn
- 2018-12-03 US US16/208,245 patent/US10774354B2/en active Active
-
2020
- 2020-08-12 US US16/991,607 patent/US20210054429A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| HK1220993A1 (en) | 2017-05-19 |
| KR20160002913A (ko) | 2016-01-08 |
| AU2014253785B2 (en) | 2018-07-19 |
| WO2014172631A4 (en) | 2015-02-19 |
| IL242148B (en) | 2019-03-31 |
| KR102289258B1 (ko) | 2021-08-11 |
| EP2986731B1 (en) | 2022-05-18 |
| WO2014172631A3 (en) | 2014-12-18 |
| US20190161781A1 (en) | 2019-05-30 |
| US20210054429A1 (en) | 2021-02-25 |
| ES2922358T3 (es) | 2022-09-13 |
| US10190145B2 (en) | 2019-01-29 |
| JP2016518833A (ja) | 2016-06-30 |
| US20140315245A1 (en) | 2014-10-23 |
| DK2986731T3 (da) | 2022-08-01 |
| CA2909516C (en) | 2023-03-07 |
| CA2909516A1 (en) | 2014-10-23 |
| CN105283556A (zh) | 2016-01-27 |
| SG10202108497TA (en) | 2021-09-29 |
| CN105283556B (zh) | 2020-07-14 |
| EP2986731A2 (en) | 2016-02-24 |
| US10774354B2 (en) | 2020-09-15 |
| JP2018157837A (ja) | 2018-10-11 |
| WO2014172631A2 (en) | 2014-10-23 |
| HUE059565T2 (hu) | 2022-11-28 |
| AU2014253785A1 (en) | 2015-11-05 |
| JP6463732B2 (ja) | 2019-02-06 |
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