KR102289258B1 - 상승된 수준의 외인성 샤페론을 갖는 세포 추출물을 이용한 박테리아 세포 비함유 합성 시스템에서의 생물학적 활성 단백질의 발현 - Google Patents

상승된 수준의 외인성 샤페론을 갖는 세포 추출물을 이용한 박테리아 세포 비함유 합성 시스템에서의 생물학적 활성 단백질의 발현 Download PDF

Info

Publication number
KR102289258B1
KR102289258B1 KR1020157032515A KR20157032515A KR102289258B1 KR 102289258 B1 KR102289258 B1 KR 102289258B1 KR 1020157032515 A KR1020157032515 A KR 1020157032515A KR 20157032515 A KR20157032515 A KR 20157032515A KR 102289258 B1 KR102289258 B1 KR 102289258B1
Authority
KR
South Korea
Prior art keywords
protein
leu
ala
glu
lys
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020157032515A
Other languages
English (en)
Korean (ko)
Other versions
KR20160002913A (ko
Inventor
앨리스 얌
댄 그로프
패트릭 리버스
크리스토퍼 디. 타노스
Original Assignee
서트로 바이오파마, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 서트로 바이오파마, 인크. filed Critical 서트로 바이오파마, 인크.
Publication of KR20160002913A publication Critical patent/KR20160002913A/ko
Application granted granted Critical
Publication of KR102289258B1 publication Critical patent/KR102289258B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y502/00—Cis-trans-isomerases (5.2)
    • C12Y502/01—Cis-trans-Isomerases (5.2.1)
    • C12Y502/01008—Peptidylprolyl isomerase (5.2.1.8), i.e. cyclophilin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/04—Intramolecular oxidoreductases (5.3) transposing S-S bonds (5.3.4)
    • C12Y503/04001—Protein disulfide-isomerase (5.3.4.1), i.e. disufide bond-forming enzyme

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020157032515A 2013-04-19 2014-04-18 상승된 수준의 외인성 샤페론을 갖는 세포 추출물을 이용한 박테리아 세포 비함유 합성 시스템에서의 생물학적 활성 단백질의 발현 Active KR102289258B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361813914P 2013-04-19 2013-04-19
US61/813,914 2013-04-19
US201461937069P 2014-02-07 2014-02-07
US61/937,069 2014-02-07
PCT/US2014/034643 WO2014172631A2 (en) 2013-04-19 2014-04-18 Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression

Publications (2)

Publication Number Publication Date
KR20160002913A KR20160002913A (ko) 2016-01-08
KR102289258B1 true KR102289258B1 (ko) 2021-08-11

Family

ID=50733442

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020157032515A Active KR102289258B1 (ko) 2013-04-19 2014-04-18 상승된 수준의 외인성 샤페론을 갖는 세포 추출물을 이용한 박테리아 세포 비함유 합성 시스템에서의 생물학적 활성 단백질의 발현

Country Status (13)

Country Link
US (3) US10190145B2 (enExample)
EP (1) EP2986731B1 (enExample)
JP (2) JP6463732B2 (enExample)
KR (1) KR102289258B1 (enExample)
CN (1) CN105283556B (enExample)
AU (1) AU2014253785B2 (enExample)
CA (1) CA2909516C (enExample)
DK (1) DK2986731T3 (enExample)
ES (1) ES2922358T3 (enExample)
HU (1) HUE059565T2 (enExample)
IL (1) IL242148B (enExample)
SG (2) SG10202108497TA (enExample)
WO (1) WO2014172631A2 (enExample)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2909516C (en) * 2013-04-19 2023-03-07 Sutro Biopharma, Inc. Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones
WO2016004024A1 (en) 2014-07-02 2016-01-07 Sutro Biopharma, Inc. High growth capacity auxotrophic escherichia coli and methods of use
US10112994B2 (en) 2014-11-05 2018-10-30 Genentech, Inc. Methods of producing two chain proteins in bacteria
EP3215525B1 (en) * 2014-11-05 2020-07-29 Genentech, Inc. Methods of producing two chain proteins in bacteria
US10487133B2 (en) 2014-12-19 2019-11-26 Sutro Biopharma, Inc. Codon optimization for titer and fidelity improvement
EP3240569A4 (en) 2014-12-30 2018-05-30 Celgene Corporation Anti-cd47 antibodies and uses thereof
ES2761726T3 (es) * 2015-03-06 2020-05-20 Hoffmann La Roche DsbA y DsbC ultrapurificadas y procedimientos de preparación y uso de las mismas
US20170029798A1 (en) * 2015-07-27 2017-02-02 Cellivery Therapeutics, Inc. Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD)
KR20180048731A (ko) * 2015-08-20 2018-05-10 제넨테크, 인크. 재조합 폴리펩티드를 제조하기 위한 fkpa의 정제 및 그의 용도
CN105198983B (zh) * 2015-09-11 2018-11-06 中国石油大学(华东) 一种利于七次跨膜蛋白ccr5功能性表达的方法
CN106190940B (zh) * 2016-07-19 2019-06-14 北京三元基因药业股份有限公司 表达抗TNF抗体Fab片段的重组大肠杆菌工程菌
CN106399352B (zh) * 2016-11-09 2020-04-17 华东理工大学 调节目标蛋白表达的折叠因子及其应用
US12227778B2 (en) 2016-12-30 2025-02-18 Nature's Toolbox, Inc. Cell-free expression system having novel inorganic polyphosphate-based energy regeneration
KR20190103256A (ko) 2016-12-30 2019-09-04 수트로박스, 인코포레이티드 비자연 아미노산을 갖는 폴리펩타이드-항원 접합체
WO2018126287A1 (en) 2016-12-30 2018-07-05 Ntxbio, Llc Cell-free expression system having novel inorganic polyphosphate-based energy regeneration
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
CA3069321A1 (en) 2017-07-11 2019-01-17 Synthorx, Inc. Incorporation of unnatural nucleotides and methods thereof
US20200181220A1 (en) 2017-08-03 2020-06-11 Synthorx, Inc. Cytokine conjugates for the treatment of proliferative and infectious diseases
JP2020533018A (ja) * 2017-08-11 2020-11-19 シンビトロバイオ， インコーポレイテッド 改善されたインビトロ転写／翻訳（ｔｘｔｌ）システムおよびその使用
WO2019127469A1 (zh) * 2017-12-29 2019-07-04 康码（上海）生物科技有限公司 一种高效的能源再生体系(bes)、试剂盒及其制备方法
TW202442671A (zh) 2018-02-26 2024-11-01 美商欣爍克斯公司 Il-15結合物及其用途
CN118147029A (zh) * 2018-07-11 2024-06-07 阿克蒂姆治疗有限公司 工程化的免疫刺激性细菌菌株及其用途
KR20210090645A (ko) * 2018-11-05 2021-07-20 제넨테크, 인크. 원핵 숙주 세포에서 2개의 사슬 단백질을 생산하는 방법
MA54195A (fr) 2018-11-08 2022-02-16 Synthorx Inc Conjugués d'interleukine 10 et leurs utilisations
CA3117992A1 (en) * 2018-11-08 2020-05-14 Sutro Biopharma, Inc. E coli strains having an oxidative cytoplasm
CN109371049A (zh) * 2018-11-14 2019-02-22 天津大学 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用
US20200246467A1 (en) 2019-02-06 2020-08-06 Synthorx, Inc. Il-2 conjugates and methods of use thereof
US12024709B2 (en) 2019-02-27 2024-07-02 Actym Therapeutics, Inc. Immunostimulatory bacteria engineered to colonize tumors, tumor-resident immune cells, and the tumor microenvironment
WO2021178001A1 (en) * 2020-03-05 2021-09-10 Curie Co. Inc. Methods for cell free protein expression of mature polypeptides derived from zymogens and proproteins
EP4013454A1 (en) 2019-08-15 2022-06-22 Synthorx, Inc. Immuno oncology combination therapies with il-2 conjugates
CA3150082A1 (en) 2019-09-10 2021-03-18 Jerod PTACIN Il-2 conjugates and methods of use to treat autoimmune diseases
AU2020380275A1 (en) 2019-11-04 2022-04-14 Synthorx, Inc. Interleukin 10 conjugates and uses thereof
EP4056584A4 (en) * 2019-11-08 2024-06-05 Korea Institute of Ocean Science & Technology NOVEL HUMAN EPIDERMAL GROWTH FACTOR-TF FUSION PROTEIN AND USE THEREOF
EP4142711A1 (en) * 2020-04-29 2023-03-08 Willow Biosciences Inc. Compositions and methods for enhancing recombinant biosynthesis of cannabinoids
JP2023531509A (ja) 2020-06-25 2023-07-24 シンソークス， インコーポレイテッド Ｉｌ－２コンジュゲートおよび抗ｅｇｆｒ抗体を用いる免疫腫瘍学併用療法
KR20220000028U (ko) 2020-06-29 2022-01-05 채인자 농작물용 농약 간편 분무장치
EP4211156A1 (en) 2020-09-14 2023-07-19 Sutro Biopharma, Inc. Method for large scale production of antibodies using a cell-free protein synthesis system
BR112023006364A2 (pt) 2020-10-09 2023-05-09 Synthorx Inc Terapia de combinação de imuno-oncologia com conjugados de il-2 e pembrolizumabe
EP4225375A1 (en) 2020-10-09 2023-08-16 Synthorx, Inc. Immuno oncology therapies with il-2 conjugates
WO2022174101A1 (en) 2021-02-12 2022-08-18 Synthorx, Inc. Skin cancer combination therapy with il-2 conjugates and cemiplimab
WO2022174102A1 (en) 2021-02-12 2022-08-18 Synthorx, Inc. Lung cancer combination therapy with il-2 conjugates and an anti-pd-1 antibody or antigen-binding fragment thereof
WO2022256534A1 (en) 2021-06-03 2022-12-08 Synthorx, Inc. Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab
EP4452327A1 (en) 2021-12-20 2024-10-30 Synthorx, Inc. Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab
WO2023122750A1 (en) 2021-12-23 2023-06-29 Synthorx, Inc. Cancer combination therapy with il-2 conjugates and cetuximab
AU2023295552A1 (en) * 2022-06-17 2024-12-12 Insitro, Inc. In situ sequencing of rna transcripts with non-uniform 5' ends
CN115028691B (zh) * 2022-06-29 2024-09-10 青岛硕景生物科技有限公司 一种检测试纸用的独立质控系统、杂交瘤细胞株、质控线用包被单克隆抗体及其应用
WO2024136899A1 (en) 2022-12-21 2024-06-27 Synthorx, Inc. Cancer therapy with il-2 conjugates and chimeric antigen receptor therapies
TW202444349A (zh) 2023-03-20 2024-11-16 美商欣爍克斯公司 使用ｉｌ－２綴合物之癌症療法
CN116925196B (zh) * 2023-06-08 2025-04-18 齐鲁工业大学(山东省科学院) 小分子热休克蛋白rsp_1572在提高宿主菌环境耐受性中的应用
WO2025158385A1 (en) 2024-01-25 2025-07-31 Genzyme Corporation Pegylated il-2 for suppressing adaptive immune response to gene therapy
CN118048325B (zh) * 2024-04-16 2024-07-16 天津凯莱英生物科技有限公司 酶的高通量筛选方法及酶库的构建方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004081033A2 (en) 2003-03-11 2004-09-23 National Institutes Of Health Synthesis of proteins by cell-free protein expression
JP2005253432A (ja) 2004-03-15 2005-09-22 Sekisui Chem Co Ltd タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット
JP2006061080A (ja) 2004-08-27 2006-03-09 Toyota Central Res & Dev Lab Inc 無細胞タンパク質合成系のための媒体

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10145694A1 (de) * 2001-09-17 2003-04-03 Roche Diagnostics Gmbh Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung
EP2035554B1 (en) * 2006-06-29 2013-04-24 The Board of Trustees of The Leland Stanford Junior University Cell-free synthesis of proteins containing unnatural amino acids
WO2008088884A2 (en) * 2007-01-18 2008-07-24 The Board Of Trustees Of The Leland Stanford Junior University Enhanced cell-free synthesis of active proteins containing disulfide bonds
EP2376647B1 (en) * 2009-01-12 2014-03-05 Sutro Biopharma, Inc. Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system
CN102732548A (zh) * 2012-05-16 2012-10-17 中国农业大学 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用
KR102018863B1 (ko) * 2012-10-12 2019-09-05 서트로 바이오파마, 인크. 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화
CA2909516C (en) * 2013-04-19 2023-03-07 Sutro Biopharma, Inc. Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004081033A2 (en) 2003-03-11 2004-09-23 National Institutes Of Health Synthesis of proteins by cell-free protein expression
JP2005253432A (ja) 2004-03-15 2005-09-22 Sekisui Chem Co Ltd タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット
JP2006061080A (ja) 2004-08-27 2006-03-09 Toyota Central Res & Dev Lab Inc 無細胞タンパク質合成系のための媒体

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Bioprocess Biosyst., Vol. 33, pp. 127-132 (2010.)

Also Published As

Publication number Publication date
SG10202108497TA (en) 2021-09-29
EP2986731A2 (en) 2016-02-24
ES2922358T3 (es) 2022-09-13
HUE059565T2 (hu) 2022-11-28
WO2014172631A3 (en) 2014-12-18
SG11201508642WA (en) 2015-11-27
DK2986731T3 (da) 2022-08-01
EP2986731B1 (en) 2022-05-18
CA2909516A1 (en) 2014-10-23
HK1220993A1 (en) 2017-05-19
IL242148B (en) 2019-03-31
WO2014172631A4 (en) 2015-02-19
AU2014253785A1 (en) 2015-11-05
CN105283556B (zh) 2020-07-14
US20140315245A1 (en) 2014-10-23
CN105283556A (zh) 2016-01-27
JP2016518833A (ja) 2016-06-30
US20210054429A1 (en) 2021-02-25
JP2018157837A (ja) 2018-10-11
US10774354B2 (en) 2020-09-15
CA2909516C (en) 2023-03-07
US20190161781A1 (en) 2019-05-30
WO2014172631A2 (en) 2014-10-23
US10190145B2 (en) 2019-01-29
JP6463732B2 (ja) 2019-02-06
KR20160002913A (ko) 2016-01-08
AU2014253785B2 (en) 2018-07-19

Similar Documents

Publication Publication Date Title
KR102289258B1 (ko) 상승된 수준의 외인성 샤페론을 갖는 세포 추출물을 이용한 박테리아 세포 비함유 합성 시스템에서의 생물학적 활성 단백질의 발현
US11261219B2 (en) Proteolytic inactivation of select proteins in bacterial extracts for improved expression
US10487133B2 (en) Codon optimization for titer and fidelity improvement
US20200332280A1 (en) Use of lambda-gam protein in ribosomal display technology
US20220089690A1 (en) Method for large scale production of antibodies using a cell-free protein synthesis system
HK1220993B (en) Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones
TWI899326B (zh) 使用無細胞蛋白質合成系統來大規模生產抗體之方法

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20151112

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20190418

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20201210

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20210616

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20210806

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20210806

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20240627

Start annual number: 4

End annual number: 4