DK2986731T3 - Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner - Google Patents
Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner Download PDFInfo
- Publication number
- DK2986731T3 DK2986731T3 DK14724974.2T DK14724974T DK2986731T3 DK 2986731 T3 DK2986731 T3 DK 2986731T3 DK 14724974 T DK14724974 T DK 14724974T DK 2986731 T3 DK2986731 T3 DK 2986731T3
- Authority
- DK
- Denmark
- Prior art keywords
- expression
- biologically active
- elevated levels
- synthesis system
- active proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361813914P | 2013-04-19 | 2013-04-19 | |
| US201461937069P | 2014-02-07 | 2014-02-07 | |
| PCT/US2014/034643 WO2014172631A2 (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2986731T3 true DK2986731T3 (da) | 2022-08-01 |
Family
ID=50733442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14724974.2T DK2986731T3 (da) | 2013-04-19 | 2014-04-18 | Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US10190145B2 (da) |
| EP (1) | EP2986731B1 (da) |
| JP (2) | JP6463732B2 (da) |
| KR (1) | KR102289258B1 (da) |
| CN (1) | CN105283556B (da) |
| AU (1) | AU2014253785B2 (da) |
| CA (1) | CA2909516C (da) |
| DK (1) | DK2986731T3 (da) |
| ES (1) | ES2922358T3 (da) |
| HK (1) | HK1220993A1 (da) |
| HU (1) | HUE059565T2 (da) |
| IL (1) | IL242148B (da) |
| SG (2) | SG10202108497TA (da) |
| WO (1) | WO2014172631A2 (da) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2986731T3 (da) * | 2013-04-19 | 2022-08-01 | Sutro Biopharma Inc | Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner |
| WO2016004024A1 (en) * | 2014-07-02 | 2016-01-07 | Sutro Biopharma, Inc. | High growth capacity auxotrophic escherichia coli and methods of use |
| WO2016073791A1 (en) | 2014-11-05 | 2016-05-12 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
| KR102544705B1 (ko) | 2014-11-05 | 2023-06-15 | 제넨테크, 인크. | 박테리아 내 2쇄 단백질을 생산하는 방법 |
| EP3234170A4 (en) * | 2014-12-19 | 2018-07-25 | Sutro Biopharma, Inc. | Codon optimization for titer and fidelity improvement |
| KR102489471B1 (ko) * | 2014-12-30 | 2023-01-18 | 셀진 코포레이션 | 항-cd47 항체 및 그 용도 |
| AU2016230018A1 (en) * | 2015-03-06 | 2017-09-21 | Genentech, Inc. | Ultrapurified DsbA and DsbC and methods of making and using the same |
| US20170029798A1 (en) * | 2015-07-27 | 2017-02-02 | Cellivery Therapeutics, Inc. | Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD) |
| BR112018003127A2 (pt) * | 2015-08-20 | 2018-09-25 | Genentech Inc | purificação de fkpa e usos do mesmo para produzir polipeptídeos recombinantes |
| CN105198983B (zh) * | 2015-09-11 | 2018-11-06 | 中国石油大学(华东) | 一种利于七次跨膜蛋白ccr5功能性表达的方法 |
| CN106190940B (zh) * | 2016-07-19 | 2019-06-14 | 北京三元基因药业股份有限公司 | 表达抗TNF抗体Fab片段的重组大肠杆菌工程菌 |
| CN106399352B (zh) * | 2016-11-09 | 2020-04-17 | 华东理工大学 | 调节目标蛋白表达的折叠因子及其应用 |
| JP7186166B2 (ja) | 2016-12-30 | 2022-12-08 | バックスサイト・インコーポレイテッド | 非天然アミノ酸とのポリペプチド抗原接合体 |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| WO2018126287A1 (en) * | 2016-12-30 | 2018-07-05 | Ntxbio, Llc | Cell-free expression system having novel inorganic polyphosphate-based energy regeneration |
| AR112756A1 (es) | 2017-07-11 | 2019-12-11 | Synthorx Inc | Incorporación de nucleótidos no naturales, y su método |
| KR20200035092A (ko) | 2017-08-03 | 2020-04-01 | 신톡스, 인크. | 자가면역 질환의 치료를 위한 사이토카인 접합체 |
| JP2020533018A (ja) * | 2017-08-11 | 2020-11-19 | シンビトロバイオ， インコーポレイテッド | 改善されたインビトロ転写／翻訳（ｔｘｔｌ）システムおよびその使用 |
| WO2019127469A1 (zh) * | 2017-12-29 | 2019-07-04 | 康码（上海）生物科技有限公司 | 一种高效的能源再生体系(bes)、试剂盒及其制备方法 |
| JP7340591B2 (ja) | 2018-07-11 | 2023-09-07 | アクティム・セラピューティクス・インコーポレイテッド | 遺伝子操作された免疫刺激性細菌菌株およびその使用 |
| JP2022506156A (ja) * | 2018-11-05 | 2022-01-17 | ジェネンテック， インコーポレイテッド | 原核宿主細胞における２鎖タンパク質の産生方法 |
| JP7426389B2 (ja) * | 2018-11-08 | 2024-02-01 | ストロ バイオファーマ， インコーポレイテッド | 酸化的細胞質を有する大腸菌株 |
| CN109371049A (zh) * | 2018-11-14 | 2019-02-22 | 天津大学 | 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用 |
| TW202045208A (zh) | 2019-02-06 | 2020-12-16 | 美商欣爍克斯公司 | Il-2結合物及其使用方法 |
| TW202120128A (zh) | 2019-08-15 | 2021-06-01 | 美商欣爍克斯公司 | 使用ｉｌ－２接合物之免疫腫瘤學組合療法 |
| BR112022003335A2 (pt) | 2019-09-10 | 2022-05-24 | Synthorx Inc | Conjugados de il-2 e métodos de uso para tratar doenças autoimunes |
| TW202131952A (zh) | 2019-11-04 | 2021-09-01 | 美商欣爍克斯公司 | 介白素10接合物及其用途 |
| EP4114931A4 (en) * | 2020-03-05 | 2024-05-01 | Curie Co. Inc. | METHODS FOR CELL PROTEIN EXPRESSION OF MATURE POLYPEPTIDES DERIVED FROM ZYMOGENS AND PROPROTEINS |
| CA3175683A1 (en) * | 2020-04-29 | 2021-11-04 | Xueyang FENG | Compositions and methods for enhancing recombinant biosynthesis of cannabinoids |
| CA3183834A1 (en) | 2020-06-25 | 2021-12-30 | Giovanni Abbadessa | Immuno oncology combination therapy with il-2 conjugates and anti-egfr antibodies |
| KR20220000028U (ko) | 2020-06-29 | 2022-01-05 | 채인자 | 농작물용 농약 간편 분무장치 |
| US20220089690A1 (en) * | 2020-09-14 | 2022-03-24 | Sutro Biopharma, Inc. | Method for large scale production of antibodies using a cell-free protein synthesis system |
| EP4225376A1 (en) | 2020-10-09 | 2023-08-16 | Synthorx, Inc. | Immuno oncology combination therapy with il-2 conjugates and pembrolizumab |
| CA3194880A1 (en) | 2020-10-09 | 2022-04-14 | Carolina E. CAFFARO | Immuno oncology therapies with il-2 conjugates |
| WO2022174102A1 (en) | 2021-02-12 | 2022-08-18 | Synthorx, Inc. | Lung cancer combination therapy with il-2 conjugates and an anti-pd-1 antibody or antigen-binding fragment thereof |
| TW202245843A (zh) | 2021-02-12 | 2022-12-01 | 美商欣爍克斯公司 | Ｉｌ－２接合物及西米普利單抗（ｃｅｍｉｐｌｉｍａｂ）之皮膚癌組合療法 |
| WO2022256534A1 (en) | 2021-06-03 | 2022-12-08 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122573A1 (en) | 2021-12-20 | 2023-06-29 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122750A1 (en) | 2021-12-23 | 2023-06-29 | Synthorx, Inc. | Cancer combination therapy with il-2 conjugates and cetuximab |
| CN115028691A (zh) * | 2022-06-29 | 2022-09-09 | 青岛硕景生物科技有限公司 | 一种检测试纸用的独立质控系统、杂交瘤细胞株、质控线用包被单克隆抗体及其应用 |
| CN118048325A (zh) * | 2024-04-16 | 2024-05-17 | 天津凯莱英生物科技有限公司 | 酶的高通量筛选方法及酶库的构建方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10145694A1 (de) | 2001-09-17 | 2003-04-03 | Roche Diagnostics Gmbh | Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung |
| US20060134739A1 (en) * | 2003-03-11 | 2006-06-22 | Chatterjee Deb K | Synthesis of proteins by cell-free protein expression |
| JP2005253432A (ja) * | 2004-03-15 | 2005-09-22 | Sekisui Chem Co Ltd | タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット |
| JP4561243B2 (ja) * | 2004-08-27 | 2010-10-13 | 株式会社豊田中央研究所 | 無細胞タンパク質合成系のための媒体 |
| CN101479379B (zh) * | 2006-06-29 | 2012-01-18 | 利兰·斯坦福青年大学托管委员会 | 含有非天然氨基酸的蛋白质的无细胞合成 |
| CA2673765A1 (en) * | 2007-01-18 | 2008-07-24 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced cell-free synthesis of active proteins containing disulfide bonds |
| WO2010081110A1 (en) | 2009-01-12 | 2010-07-15 | Sutro Biopharma, Inc. | Dual charging system for selectively introducing non-native amino acids into proteins using an in vitro synthesis method |
| CN102732548A (zh) | 2012-05-16 | 2012-10-17 | 中国农业大学 | 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用 |
| DK2986731T3 (da) | 2013-04-19 | 2022-08-01 | Sutro Biopharma Inc | Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner |
-
2014
- 2014-04-18 DK DK14724974.2T patent/DK2986731T3/da active
- 2014-04-18 EP EP14724974.2A patent/EP2986731B1/en active Active
- 2014-04-18 CA CA2909516A patent/CA2909516C/en active Active
- 2014-04-18 US US14/256,324 patent/US10190145B2/en active Active
- 2014-04-18 JP JP2016509127A patent/JP6463732B2/ja active Active
- 2014-04-18 CN CN201480032258.8A patent/CN105283556B/zh active Active
- 2014-04-18 WO PCT/US2014/034643 patent/WO2014172631A2/en active Application Filing
- 2014-04-18 SG SG10202108497TA patent/SG10202108497TA/en unknown
- 2014-04-18 SG SG11201508642WA patent/SG11201508642WA/en unknown
- 2014-04-18 ES ES14724974T patent/ES2922358T3/es active Active
- 2014-04-18 HU HUE14724974A patent/HUE059565T2/hu unknown
- 2014-04-18 KR KR1020157032515A patent/KR102289258B1/ko active IP Right Grant
- 2014-04-18 AU AU2014253785A patent/AU2014253785B2/en active Active
-
2015
- 2015-10-18 IL IL242148A patent/IL242148B/en active IP Right Grant
-
2016
- 2016-08-01 HK HK16109141.1A patent/HK1220993A1/zh unknown
-
2018
- 2018-07-20 JP JP2018136909A patent/JP2018157837A/ja not_active Withdrawn
- 2018-12-03 US US16/208,245 patent/US10774354B2/en active Active
-
2020
- 2020-08-12 US US16/991,607 patent/US20210054429A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2986731A2 (en) | 2016-02-24 |
| AU2014253785B2 (en) | 2018-07-19 |
| US20140315245A1 (en) | 2014-10-23 |
| KR20160002913A (ko) | 2016-01-08 |
| KR102289258B1 (ko) | 2021-08-11 |
| JP2018157837A (ja) | 2018-10-11 |
| US20210054429A1 (en) | 2021-02-25 |
| AU2014253785A1 (en) | 2015-11-05 |
| US20190161781A1 (en) | 2019-05-30 |
| CA2909516A1 (en) | 2014-10-23 |
| SG11201508642WA (en) | 2015-11-27 |
| ES2922358T3 (es) | 2022-09-13 |
| CN105283556B (zh) | 2020-07-14 |
| CA2909516C (en) | 2023-03-07 |
| HUE059565T2 (hu) | 2022-11-28 |
| JP6463732B2 (ja) | 2019-02-06 |
| EP2986731B1 (en) | 2022-05-18 |
| JP2016518833A (ja) | 2016-06-30 |
| CN105283556A (zh) | 2016-01-27 |
| WO2014172631A2 (en) | 2014-10-23 |
| US10774354B2 (en) | 2020-09-15 |
| IL242148B (en) | 2019-03-31 |
| WO2014172631A3 (en) | 2014-12-18 |
| WO2014172631A4 (en) | 2015-02-19 |
| SG10202108497TA (en) | 2021-09-29 |
| US10190145B2 (en) | 2019-01-29 |
| HK1220993A1 (zh) | 2017-05-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK2986731T3 (da) | Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner | |
| CL2016001190A1 (es) | Cultivo de células de mamífero. | |
| BR112015030946A2 (pt) | composição compreendendo uma célula ß pancreática não-nativa, método de diferenciação de células progenitoras em células ß pancreáticas in vitro e uso de uma célula ß pancreática não-nativa | |
| DK3351620T3 (da) | Metabolsk optimeret cellekultur | |
| DK2780022T3 (da) | Farmaceutiske præparater af humane rpe-celler og anvendelser deraf | |
| SMT201500055B (it) | Dispositivi e metodi per la coltura di cellule | |
| GB2561496A (en) | Devices and methods for simulating a function of a liver tissue | |
| DK2705143T3 (da) | Protein-induceret pluripotent celleteknologi og anvendelse deraf | |
| EP2811011A4 (en) | CELL CULTURE KIT AND METHOD FOR USING THE CELL CULTURE KIT | |
| DK2998289T3 (da) | Forbindelser til at målrette lægemiddellevering og fremme sirna-aktivitet | |
| SG2014013676A (en) | Therapeutics using adipose cells and cell secretions | |
| DK2718416T3 (da) | Ekspansion af stamceller i hulfiberbioreaktorer | |
| EP2968549A4 (en) | CELL CULTURE METHODS | |
| UY34017A (es) | Proteínas de fusión y vacunas de combinación | |
| EP2971040A4 (en) | CELL CULTURE METHODS | |
| EP2971014A4 (en) | CELL CULTURE METHODS | |
| DK2744456T3 (da) | Forbedringer ved eller i forbindelse med proteser og ortoser | |
| CO6870031A2 (es) | Pirrolotriazinas sustituidas con hidroximetilarilo y sus usos | |
| CO6870003A2 (es) | Vacunas bovinas y métodos | |
| UY34124A (es) | Cerramiento y método para conformarlo | |
| BR112014002624A2 (pt) | microorganismo recombinante e cultura de microorganismo recombinante | |
| DK2753346T3 (da) | Ceramidase og celledifferentiering | |
| BR112013033166A2 (pt) | método microbiológico de produção de uma solução de dextrano, solução de dextrano nativo e seu uso | |
| DK2938186T3 (da) | Organ- og vævsbevaringsformuleringer med øget stabilitet og holdbarhed | |
| SG10201806083RA (en) | Microcarrier perfusion culturing methods and uses thereof |