DK2986731T3 - Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner - Google Patents
Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner Download PDFInfo
- Publication number
- DK2986731T3 DK2986731T3 DK14724974.2T DK14724974T DK2986731T3 DK 2986731 T3 DK2986731 T3 DK 2986731T3 DK 14724974 T DK14724974 T DK 14724974T DK 2986731 T3 DK2986731 T3 DK 2986731T3
- Authority
- DK
- Denmark
- Prior art keywords
- expression
- biologically active
- elevated levels
- synthesis system
- active proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361813914P | 2013-04-19 | 2013-04-19 | |
| US201461937069P | 2014-02-07 | 2014-02-07 | |
| PCT/US2014/034643 WO2014172631A2 (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2986731T3 true DK2986731T3 (da) | 2022-08-01 |
Family
ID=50733442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14724974.2T DK2986731T3 (da) | 2013-04-19 | 2014-04-18 | Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US10190145B2 (da) |
| EP (1) | EP2986731B1 (da) |
| JP (2) | JP6463732B2 (da) |
| KR (1) | KR102289258B1 (da) |
| CN (1) | CN105283556B (da) |
| AU (1) | AU2014253785B2 (da) |
| CA (1) | CA2909516C (da) |
| DK (1) | DK2986731T3 (da) |
| ES (1) | ES2922358T3 (da) |
| HK (1) | HK1220993A1 (da) |
| HU (1) | HUE059565T2 (da) |
| IL (1) | IL242148B (da) |
| SG (2) | SG10202108497TA (da) |
| WO (1) | WO2014172631A2 (da) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG10202108497TA (en) * | 2013-04-19 | 2021-09-29 | Sutro Biopharma Inc | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
| EP3164501A4 (en) | 2014-07-02 | 2018-01-17 | Sutro Biopharma, Inc. | High growth capacity auxotrophic escherichia coli and methods of use |
| US10066002B2 (en) * | 2014-11-05 | 2018-09-04 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
| CA2966558C (en) | 2014-11-05 | 2024-03-12 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
| US10487133B2 (en) | 2014-12-19 | 2019-11-26 | Sutro Biopharma, Inc. | Codon optimization for titer and fidelity improvement |
| SG11201705310TA (en) * | 2014-12-30 | 2017-07-28 | Celgene Corp | Anti-cd47 antibodies and uses thereof |
| EP3636749B1 (en) * | 2015-03-06 | 2022-04-27 | F. Hoffmann-La Roche AG | Ultrapurified dsbc and methods of making and using the same |
| US20170029798A1 (en) * | 2015-07-27 | 2017-02-02 | Cellivery Therapeutics, Inc. | Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD) |
| CA2995385A1 (en) * | 2015-08-20 | 2017-02-23 | Genentech, Inc. | Purification of fkpa and uses thereof for producing recombinant polypeptides |
| CN105198983B (zh) * | 2015-09-11 | 2018-11-06 | 中国石油大学(华东) | 一种利于七次跨膜蛋白ccr5功能性表达的方法 |
| CN106190940B (zh) * | 2016-07-19 | 2019-06-14 | 北京三元基因药业股份有限公司 | 表达抗TNF抗体Fab片段的重组大肠杆菌工程菌 |
| CN106399352B (zh) * | 2016-11-09 | 2020-04-17 | 华东理工大学 | 调节目标蛋白表达的折叠因子及其应用 |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| EP3562940B1 (en) * | 2016-12-30 | 2022-06-15 | Nature's Toolbox, Inc. | Cell-free expression system having novel inorganic polyphosphate-based energy regeneration |
| MA49578A (fr) | 2017-07-11 | 2021-04-07 | Synthorx Inc | Incorporation de nucléotides non naturels et procédés associés |
| WO2019028419A1 (en) | 2017-08-03 | 2019-02-07 | Synthorx, Inc. | CYTOKINE CONJUGATES FOR THE TREATMENT OF PROLIFERATIVE AND INFECTIOUS DISEASES |
| EP3665188A4 (en) * | 2017-08-11 | 2021-07-21 | Synvitrobio, Inc. | IMPROVED IN VITRO TRANSLATION / TRANSLATION SYSTEM (TXTL) AND ITS USES |
| WO2019127469A1 (zh) * | 2017-12-29 | 2019-07-04 | 康码（上海）生物科技有限公司 | 一种高效的能源再生体系(bes)、试剂盒及其制备方法 |
| SG11202100023XA (en) | 2018-07-11 | 2021-01-28 | Actym Therapeutics Inc | Engineered immunostimulatory bacterial strains and uses thereof |
| TW202031899A (zh) * | 2018-11-05 | 2020-09-01 | 美商建南德克公司 | 在原核宿主細胞中產生二鏈蛋白質之方法 |
| CA3117992A1 (en) * | 2018-11-08 | 2020-05-14 | Sutro Biopharma, Inc. | E coli strains having an oxidative cytoplasm |
| CN109371049A (zh) * | 2018-11-14 | 2019-02-22 | 天津大学 | 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用 |
| CA3127689A1 (en) | 2019-02-06 | 2020-08-13 | Synthorx, Inc. | Il-2 conjugates and methods of use thereof |
| WO2021178001A1 (en) * | 2020-03-05 | 2021-09-10 | Curie Co. Inc. | Methods for cell free protein expression of mature polypeptides derived from zymogens and proproteins |
| CA3150163A1 (en) | 2019-08-15 | 2021-02-18 | Synthorx, Inc. | Immuno oncology combination therapies with il-2 conjugates |
| CN114746122A (zh) | 2019-09-10 | 2022-07-12 | 新索思股份有限公司 | Il-2缀合物和治疗自身免疫性疾病的使用方法 |
| AU2020380275A1 (en) | 2019-11-04 | 2022-04-14 | Synthorx, Inc. | Interleukin 10 conjugates and uses thereof |
| EP4142711A1 (en) * | 2020-04-29 | 2023-03-08 | Willow Biosciences Inc. | Compositions and methods for enhancing recombinant biosynthesis of cannabinoids |
| EP4171648A1 (en) | 2020-06-25 | 2023-05-03 | Synthorx, Inc. | Immuno oncology combination therapy with il-2 conjugates and anti-egfr antibodies |
| KR20220000028U (ko) | 2020-06-29 | 2022-01-05 | 채인자 | 농작물용 농약 간편 분무장치 |
| EP4211156A1 (en) * | 2020-09-14 | 2023-07-19 | Sutro Biopharma, Inc. | Method for large scale production of antibodies using a cell-free protein synthesis system |
| CA3194859A1 (en) | 2020-10-09 | 2022-04-14 | Carolina E. CAFFARO | Immuno oncology combination therapy with il-2 conjugates and pembrolizumab |
| IL301612A (en) | 2020-10-09 | 2023-05-01 | Synthorx Inc | Immuno-oncological treatments with IL-2 couples |
| WO2022174101A1 (en) | 2021-02-12 | 2022-08-18 | Synthorx, Inc. | Skin cancer combination therapy with il-2 conjugates and cemiplimab |
| TW202302148A (zh) | 2021-02-12 | 2023-01-16 | 美商欣爍克斯公司 | 使用ｉｌ－２接合物和抗ｐｄ－１抗體或其抗原結合片段的肺癌組合療法 |
| EP4346903A1 (en) | 2021-06-03 | 2024-04-10 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122573A1 (en) | 2021-12-20 | 2023-06-29 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122750A1 (en) | 2021-12-23 | 2023-06-29 | Synthorx, Inc. | Cancer combination therapy with il-2 conjugates and cetuximab |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10145694A1 (de) * | 2001-09-17 | 2003-04-03 | Roche Diagnostics Gmbh | Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung |
| US20060134739A1 (en) * | 2003-03-11 | 2006-06-22 | Chatterjee Deb K | Synthesis of proteins by cell-free protein expression |
| JP2005253432A (ja) | 2004-03-15 | 2005-09-22 | Sekisui Chem Co Ltd | タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット |
| JP4561243B2 (ja) | 2004-08-27 | 2010-10-13 | 株式会社豊田中央研究所 | 無細胞タンパク質合成系のための媒体 |
| AU2007325952B8 (en) * | 2006-06-29 | 2013-06-27 | The Board Of Trustees Of The Leland Stanford Junior University | Cell-free synthesis of proteins containing unnatural amino acids |
| JP2010516251A (ja) * | 2007-01-18 | 2010-05-20 | ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ | ジスルフィド結合を含む活性なタンパク質の増強された無細胞合成の方法 |
| EP2379578A4 (en) * | 2009-01-12 | 2012-05-02 | Sutro Biopharma Inc | DOUBLE-LOADING SYSTEM FOR THE SELECTIVE INTRODUCTION OF NON-NITIVE AMINO ACIDS IN PROTEINS DUE TO AN IN VITRO-SYNTHESIS PROCESS |
| CN102732548A (zh) * | 2012-05-16 | 2012-10-17 | 中国农业大学 | 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用 |
| SG10202108497TA (en) * | 2013-04-19 | 2021-09-29 | Sutro Biopharma Inc | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
-
2014
- 2014-04-18 SG SG10202108497TA patent/SG10202108497TA/en unknown
- 2014-04-18 JP JP2016509127A patent/JP6463732B2/ja active Active
- 2014-04-18 AU AU2014253785A patent/AU2014253785B2/en active Active
- 2014-04-18 HU HUE14724974A patent/HUE059565T2/hu unknown
- 2014-04-18 ES ES14724974T patent/ES2922358T3/es active Active
- 2014-04-18 CN CN201480032258.8A patent/CN105283556B/zh active Active
- 2014-04-18 SG SG11201508642WA patent/SG11201508642WA/en unknown
- 2014-04-18 EP EP14724974.2A patent/EP2986731B1/en active Active
- 2014-04-18 KR KR1020157032515A patent/KR102289258B1/ko active IP Right Grant
- 2014-04-18 WO PCT/US2014/034643 patent/WO2014172631A2/en active Application Filing
- 2014-04-18 CA CA2909516A patent/CA2909516C/en active Active
- 2014-04-18 US US14/256,324 patent/US10190145B2/en active Active
- 2014-04-18 DK DK14724974.2T patent/DK2986731T3/da active
-
2015
- 2015-10-18 IL IL242148A patent/IL242148B/en active IP Right Grant
-
2016
- 2016-08-01 HK HK16109141.1A patent/HK1220993A1/zh unknown
-
2018
- 2018-07-20 JP JP2018136909A patent/JP2018157837A/ja not_active Withdrawn
- 2018-12-03 US US16/208,245 patent/US10774354B2/en active Active
-
2020
- 2020-08-12 US US16/991,607 patent/US20210054429A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2986731B1 (en) | 2022-05-18 |
| KR20160002913A (ko) | 2016-01-08 |
| AU2014253785A1 (en) | 2015-11-05 |
| JP6463732B2 (ja) | 2019-02-06 |
| KR102289258B1 (ko) | 2021-08-11 |
| US20140315245A1 (en) | 2014-10-23 |
| HK1220993A1 (zh) | 2017-05-19 |
| US20190161781A1 (en) | 2019-05-30 |
| US10190145B2 (en) | 2019-01-29 |
| CA2909516C (en) | 2023-03-07 |
| JP2016518833A (ja) | 2016-06-30 |
| US20210054429A1 (en) | 2021-02-25 |
| EP2986731A2 (en) | 2016-02-24 |
| CN105283556A (zh) | 2016-01-27 |
| SG10202108497TA (en) | 2021-09-29 |
| AU2014253785B2 (en) | 2018-07-19 |
| SG11201508642WA (en) | 2015-11-27 |
| HUE059565T2 (hu) | 2022-11-28 |
| JP2018157837A (ja) | 2018-10-11 |
| CA2909516A1 (en) | 2014-10-23 |
| IL242148B (en) | 2019-03-31 |
| CN105283556B (zh) | 2020-07-14 |
| WO2014172631A3 (en) | 2014-12-18 |
| WO2014172631A4 (en) | 2015-02-19 |
| WO2014172631A2 (en) | 2014-10-23 |
| ES2922358T3 (es) | 2022-09-13 |
| US10774354B2 (en) | 2020-09-15 |
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