CA2909516C - Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones - Google Patents

Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones Download PDF

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Publication number
CA2909516C
CA2909516C CA2909516A CA2909516A CA2909516C CA 2909516 C CA2909516 C CA 2909516C CA 2909516 A CA2909516 A CA 2909516A CA 2909516 A CA2909516 A CA 2909516A CA 2909516 C CA2909516 C CA 2909516C
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protein
extract
isomerase
exogenous
interest
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French (fr)
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CA2909516A1 (en
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Alice Yam
Dan Groff
Patrick RIVERS
Christopher D. Thanos
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Sutro Biopharma Inc
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Sutro Biopharma Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2909516A 2013-04-19 2014-04-18 Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones Active CA2909516C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361813914P 2013-04-19 2013-04-19
US61/813,914 2013-04-19
US201461937069P 2014-02-07 2014-02-07
US61/937,069 2014-02-07
PCT/US2014/034643 WO2014172631A2 (en) 2013-04-19 2014-04-18 Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression

Publications (2)

Publication Number Publication Date
CA2909516A1 CA2909516A1 (en) 2014-10-23
CA2909516C true CA2909516C (en) 2023-03-07

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CA2909516A Active CA2909516C (en) 2013-04-19 2014-04-18 Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones

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US (3) US10190145B2 (enExample)
EP (1) EP2986731B1 (enExample)
JP (2) JP6463732B2 (enExample)
KR (1) KR102289258B1 (enExample)
CN (1) CN105283556B (enExample)
AU (1) AU2014253785B2 (enExample)
CA (1) CA2909516C (enExample)
DK (1) DK2986731T3 (enExample)
ES (1) ES2922358T3 (enExample)
HU (1) HUE059565T2 (enExample)
IL (1) IL242148B (enExample)
SG (2) SG10202108497TA (enExample)
WO (1) WO2014172631A2 (enExample)

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JP2022554272A (ja) 2019-11-04 2022-12-28 シンソークス， インコーポレイテッド インターロイキン１０コンジュゲートおよびその使用
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Also Published As

Publication number Publication date
KR20160002913A (ko) 2016-01-08
JP2018157837A (ja) 2018-10-11
ES2922358T3 (es) 2022-09-13
US20190161781A1 (en) 2019-05-30
CN105283556B (zh) 2020-07-14
US20210054429A1 (en) 2021-02-25
AU2014253785B2 (en) 2018-07-19
SG11201508642WA (en) 2015-11-27
KR102289258B1 (ko) 2021-08-11
WO2014172631A4 (en) 2015-02-19
HUE059565T2 (hu) 2022-11-28
US10190145B2 (en) 2019-01-29
US20140315245A1 (en) 2014-10-23
US10774354B2 (en) 2020-09-15
DK2986731T3 (da) 2022-08-01
IL242148B (en) 2019-03-31
JP2016518833A (ja) 2016-06-30
CA2909516A1 (en) 2014-10-23
WO2014172631A3 (en) 2014-12-18
SG10202108497TA (en) 2021-09-29
EP2986731B1 (en) 2022-05-18
JP6463732B2 (ja) 2019-02-06
WO2014172631A2 (en) 2014-10-23
CN105283556A (zh) 2016-01-27
EP2986731A2 (en) 2016-02-24
HK1220993A1 (en) 2017-05-19
AU2014253785A1 (en) 2015-11-05

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