CA2909516C - Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones - Google Patents
Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones Download PDFInfo
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Classifications
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- Chemical & Material Sciences (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| US10316322B2 (en) * | 2014-07-02 | 2019-06-11 | Sutro Biopharma, Inc. | High growth capacity auxotrophic Escherichia coli and methods of use |
| ES2819256T3 (es) | 2014-11-05 | 2021-04-15 | Genentech Inc | Métodos para producir proteínas bicatenarias en bacterias |
| US10112994B2 (en) | 2014-11-05 | 2018-10-30 | Genentech, Inc. | Methods of producing two chain proteins in bacteria |
| EP3234170A4 (en) * | 2014-12-19 | 2018-07-25 | Sutro Biopharma, Inc. | Codon optimization for titer and fidelity improvement |
| EA037654B1 (ru) | 2014-12-30 | 2021-04-27 | Селджин Корпорейшн | Анти-cd47-антитела и их применения |
| EP3265557B1 (en) * | 2015-03-06 | 2019-10-16 | F. Hoffmann-La Roche AG | Ultrapurified dsba and dsbc and methods of making and using the same |
| US20170029798A1 (en) * | 2015-07-27 | 2017-02-02 | Cellivery Therapeutics, Inc. | Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD) |
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| WO2019127469A1 (zh) * | 2017-12-29 | 2019-07-04 | 康码（上海）生物科技有限公司 | 一种高效的能源再生体系(bes)、试剂盒及其制备方法 |
| MY203509A (en) | 2018-02-26 | 2024-06-30 | Synthorx Inc | Il-15 conjugates and uses thereof |
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| KR20230027235A (ko) | 2020-06-25 | 2023-02-27 | 신톡스, 인크. | Il-2 콘쥬게이트 및 항-egfr 항체를 사용한 면역 종양학 병용 요법 |
| KR20220000028U (ko) | 2020-06-29 | 2022-01-05 | 채인자 | 농작물용 농약 간편 분무장치 |
| EP4211156A1 (en) | 2020-09-14 | 2023-07-19 | Sutro Biopharma, Inc. | Method for large scale production of antibodies using a cell-free protein synthesis system |
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| TW202228786A (zh) | 2020-10-09 | 2022-08-01 | 美商欣爍克斯公司 | Il-2接合物的免疫腫瘤學療法 |
| TW202245843A (zh) | 2021-02-12 | 2022-12-01 | 美商欣爍克斯公司 | Ｉｌ－２接合物及西米普利單抗（ｃｅｍｉｐｌｉｍａｂ）之皮膚癌組合療法 |
| TW202302148A (zh) | 2021-02-12 | 2023-01-16 | 美商欣爍克斯公司 | 使用ｉｌ－２接合物和抗ｐｄ－１抗體或其抗原結合片段的肺癌組合療法 |
| EP4346903A1 (en) | 2021-06-03 | 2024-04-10 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122573A1 (en) | 2021-12-20 | 2023-06-29 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
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| AU2023295552A1 (en) * | 2022-06-17 | 2024-12-12 | Insitro, Inc. | In situ sequencing of rna transcripts with non-uniform 5' ends |
| CN115028691B (zh) * | 2022-06-29 | 2024-09-10 | 青岛硕景生物科技有限公司 | 一种检测试纸用的独立质控系统、杂交瘤细胞株、质控线用包被单克隆抗体及其应用 |
| WO2024136899A1 (en) | 2022-12-21 | 2024-06-27 | Synthorx, Inc. | Cancer therapy with il-2 conjugates and chimeric antigen receptor therapies |
| TW202444349A (zh) | 2023-03-20 | 2024-11-16 | 美商欣爍克斯公司 | 使用ｉｌ－２綴合物之癌症療法 |
| CN116925196B (zh) * | 2023-06-08 | 2025-04-18 | 齐鲁工业大学(山东省科学院) | 小分子热休克蛋白rsp_1572在提高宿主菌环境耐受性中的应用 |
| WO2025158385A1 (en) | 2024-01-25 | 2025-07-31 | Genzyme Corporation | Pegylated il-2 for suppressing adaptive immune response to gene therapy |
| CN118048325B (zh) * | 2024-04-16 | 2024-07-16 | 天津凯莱英生物科技有限公司 | 酶的高通量筛选方法及酶库的构建方法 |
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| DE10145694A1 (de) * | 2001-09-17 | 2003-04-03 | Roche Diagnostics Gmbh | Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung |
| US20060134739A1 (en) * | 2003-03-11 | 2006-06-22 | Chatterjee Deb K | Synthesis of proteins by cell-free protein expression |
| JP2005253432A (ja) * | 2004-03-15 | 2005-09-22 | Sekisui Chem Co Ltd | タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット |
| JP4561243B2 (ja) * | 2004-08-27 | 2010-10-13 | 株式会社豊田中央研究所 | 無細胞タンパク質合成系のための媒体 |
| EP2035554B1 (en) * | 2006-06-29 | 2013-04-24 | The Board of Trustees of The Leland Stanford Junior University | Cell-free synthesis of proteins containing unnatural amino acids |
| CA2673765A1 (en) * | 2007-01-18 | 2008-07-24 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced cell-free synthesis of active proteins containing disulfide bonds |
| JP5798489B2 (ja) * | 2009-01-12 | 2015-10-21 | ストロ バイオファーマ， インコーポレイテッド | インビトロタンパク質合成システムを用いて非天然アミノ酸をタンパク質に選択的に導入するためのモノチャージシステム |
| CN102732548A (zh) * | 2012-05-16 | 2012-10-17 | 中国农业大学 | 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用 |
| KR102018863B1 (ko) * | 2012-10-12 | 2019-09-05 | 서트로 바이오파마, 인크. | 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화 |
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| AU2014253785A1 (en) | 2015-11-05 |
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| US20140315245A1 (en) | 2014-10-23 |
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| SG10202108497TA (en) | 2021-09-29 |
| KR102289258B1 (ko) | 2021-08-11 |
| DK2986731T3 (da) | 2022-08-01 |
| CN105283556B (zh) | 2020-07-14 |
| JP2016518833A (ja) | 2016-06-30 |
| US10190145B2 (en) | 2019-01-29 |
| EP2986731A2 (en) | 2016-02-24 |
| JP2018157837A (ja) | 2018-10-11 |
| SG11201508642WA (en) | 2015-11-27 |
| WO2014172631A2 (en) | 2014-10-23 |
| HUE059565T2 (hu) | 2022-11-28 |
| CN105283556A (zh) | 2016-01-27 |
| EP2986731B1 (en) | 2022-05-18 |
| HK1220993A1 (en) | 2017-05-19 |
| WO2014172631A4 (en) | 2015-02-19 |
| WO2014172631A3 (en) | 2014-12-18 |
| KR20160002913A (ko) | 2016-01-08 |
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