CN105283556B - 在细菌无细胞合成系统中表达生物学活性蛋白 - Google Patents

在细菌无细胞合成系统中表达生物学活性蛋白 Download PDF

Info

Publication number
CN105283556B
CN105283556B CN201480032258.8A CN201480032258A CN105283556B CN 105283556 B CN105283556 B CN 105283556B CN 201480032258 A CN201480032258 A CN 201480032258A CN 105283556 B CN105283556 B CN 105283556B
Authority
CN
China
Prior art keywords
protein
exogenous
extract
isomerase
free
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201480032258.8A
Other languages
English (en)
Chinese (zh)
Other versions
CN105283556A (zh
Inventor
艾丽丝·扬
丹·格罗夫
帕特里克·里弗斯
克里斯托弗·D·萨诺斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sutro Biopharma Inc
Original Assignee
Sutro Biopharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sutro Biopharma Inc filed Critical Sutro Biopharma Inc
Publication of CN105283556A publication Critical patent/CN105283556A/zh
Application granted granted Critical
Publication of CN105283556B publication Critical patent/CN105283556B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201480032258.8A 2013-04-19 2014-04-18 在细菌无细胞合成系统中表达生物学活性蛋白 Active CN105283556B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361813914P 2013-04-19 2013-04-19
US61/813,914 2013-04-19
US201461937069P 2014-02-07 2014-02-07
US61/937,069 2014-02-07
PCT/US2014/034643 WO2014172631A2 (en) 2013-04-19 2014-04-18 Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression

Publications (2)

Publication Number Publication Date
CN105283556A CN105283556A (zh) 2016-01-27
CN105283556B true CN105283556B (zh) 2020-07-14

Family

ID=50733442

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480032258.8A Active CN105283556B (zh) 2013-04-19 2014-04-18 在细菌无细胞合成系统中表达生物学活性蛋白

Country Status (13)

Country Link
US (3) US10190145B2 (enExample)
EP (1) EP2986731B1 (enExample)
JP (2) JP6463732B2 (enExample)
KR (1) KR102289258B1 (enExample)
CN (1) CN105283556B (enExample)
AU (1) AU2014253785B2 (enExample)
CA (1) CA2909516C (enExample)
DK (1) DK2986731T3 (enExample)
ES (1) ES2922358T3 (enExample)
HU (1) HUE059565T2 (enExample)
IL (1) IL242148B (enExample)
SG (2) SG11201508642WA (enExample)
WO (1) WO2014172631A2 (enExample)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102289258B1 (ko) * 2013-04-19 2021-08-11 서트로 바이오파마, 인크. 상승된 수준의 외인성 샤페론을 갖는 세포 추출물을 이용한 박테리아 세포 비함유 합성 시스템에서의 생물학적 활성 단백질의 발현
EP3164501A4 (en) 2014-07-02 2018-01-17 Sutro Biopharma, Inc. High growth capacity auxotrophic escherichia coli and methods of use
RU2739500C2 (ru) 2014-11-05 2020-12-25 Дженентек, Инк. Способы получения двуцепочечных белков в бактериях
CN108064308B (zh) 2014-11-05 2023-06-09 豪夫迈·罗氏有限公司 细菌中产生双链蛋白的方法
US10487133B2 (en) 2014-12-19 2019-11-26 Sutro Biopharma, Inc. Codon optimization for titer and fidelity improvement
MX391051B (es) * 2014-12-30 2025-03-21 Celgene Corp Anticuerpos anti-cd47 y usos de los mismos.
CN107531749A (zh) * 2015-03-06 2018-01-02 豪夫迈·罗氏有限公司 超纯化DsbA和DsbC及其制备和使用方法
US20170029798A1 (en) * 2015-07-27 2017-02-02 Cellivery Therapeutics, Inc. Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD)
WO2017031476A2 (en) * 2015-08-20 2017-02-23 Genentech, Inc. Purification of fkpa and uses thereof for producing recombinant polypeptides
CN105198983B (zh) * 2015-09-11 2018-11-06 中国石油大学(华东) 一种利于七次跨膜蛋白ccr5功能性表达的方法
CN106190940B (zh) * 2016-07-19 2019-06-14 北京三元基因药业股份有限公司 表达抗TNF抗体Fab片段的重组大肠杆菌工程菌
CN106399352B (zh) * 2016-11-09 2020-04-17 华东理工大学 调节目标蛋白表达的折叠因子及其应用
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
US12227778B2 (en) 2016-12-30 2025-02-18 Nature's Toolbox, Inc. Cell-free expression system having novel inorganic polyphosphate-based energy regeneration
MX2019007910A (es) 2016-12-30 2019-12-05 Sutrovax Inc Conjugados de polipeptido-antigeno con aminoacidos no naturales.
DK3562940T3 (da) * 2016-12-30 2022-07-11 Natures Toolbox Inc Cellefrit ekspressionssystem med hidtil ukendt, uorganisk polyphosphatbaseret energiregeneration
JP7325341B2 (ja) 2017-07-11 2023-08-14 シンソークス，インク． 非天然ヌクレオチドの組み込み及びその方法
BR112020002272A2 (pt) 2017-08-03 2020-07-28 Synthorx, Inc. conjugados de citocina para o tratamento de doenças autoimunes
US20200181670A1 (en) * 2017-08-11 2020-06-11 Synvitrobio, Inc. Improved In Vitro Transcription/Translation (TXTL) System and Use Thereof
WO2019127469A1 (zh) * 2017-12-29 2019-07-04 康码（上海）生物科技有限公司 一种高效的能源再生体系(bes)、试剂盒及其制备方法
KR102812714B1 (ko) 2018-02-26 2025-05-27 신톡스, 인크. Il-15 접합체 및 이의 용도
SG11202100023XA (en) 2018-07-11 2021-01-28 Actym Therapeutics Inc Engineered immunostimulatory bacterial strains and uses thereof
WO2020096959A1 (en) 2018-11-05 2020-05-14 Genentech, Inc. Methods of producing two chain proteins in prokaryotic host cells
JP7636323B2 (ja) 2018-11-08 2025-02-26 シンソークス， インコーポレイテッド インターロイキン１０コンジュゲートおよびその使用
WO2020097385A1 (en) * 2018-11-08 2020-05-14 Sutro Biopharma, Inc. E coli strains having an oxidative cytoplasm
CN109371049A (zh) * 2018-11-14 2019-02-22 天津大学 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用
CN113660946A (zh) 2019-02-06 2021-11-16 新索思股份有限公司 Il-2缀合物及其使用方法
US12024709B2 (en) 2019-02-27 2024-07-02 Actym Therapeutics, Inc. Immunostimulatory bacteria engineered to colonize tumors, tumor-resident immune cells, and the tumor microenvironment
WO2021178001A1 (en) * 2020-03-05 2021-09-10 Curie Co. Inc. Methods for cell free protein expression of mature polypeptides derived from zymogens and proproteins
MX2022001776A (es) 2019-08-15 2022-03-17 Synthorx Inc Terapias de combinacion de inmuno oncologia con conjugados de il-2.
JP7758662B2 (ja) 2019-09-10 2025-10-22 シンソークス， インコーポレイテッド Ｉｌ－２コンジュゲートおよび自己免疫疾患を治療するための使用方法
KR20220097445A (ko) 2019-11-04 2022-07-07 신톡스, 인크. 인터류킨 10 접합체 및 이의 용도
EP4056584A4 (en) * 2019-11-08 2024-06-05 Korea Institute of Ocean Science & Technology NOVEL HUMAN EPIDERMAL GROWTH FACTOR-TF FUSION PROTEIN AND USE THEREOF
CA3175683A1 (en) * 2020-04-29 2021-11-04 Xueyang FENG Compositions and methods for enhancing recombinant biosynthesis of cannabinoids
EP4171648A1 (en) 2020-06-25 2023-05-03 Synthorx, Inc. Immuno oncology combination therapy with il-2 conjugates and anti-egfr antibodies
KR20220000028U (ko) 2020-06-29 2022-01-05 채인자 농작물용 농약 간편 분무장치
AU2021342284A1 (en) * 2020-09-14 2023-04-20 Sutro Biopharma, Inc. Method for large scale production of antibodies using a cell-free protein synthesis system
MX2023004029A (es) 2020-10-09 2023-04-27 Synthorx Inc Terapia de combinacion de inmunoncologia con conjugados de il-2 y pembrolizumab.
EP4225375A1 (en) 2020-10-09 2023-08-16 Synthorx, Inc. Immuno oncology therapies with il-2 conjugates
EP4291243A1 (en) 2021-02-12 2023-12-20 Synthorx, Inc. Lung cancer combination therapy with il-2 conjugates and an anti-pd-1 antibody or antigen-binding fragment thereof
TW202245843A (zh) 2021-02-12 2022-12-01 美商欣爍克斯公司 Ｉｌ－２接合物及西米普利單抗（ｃｅｍｉｐｌｉｍａｂ）之皮膚癌組合療法
TW202313117A (zh) 2021-06-03 2023-04-01 美商欣爍克斯公司 包含il-2接合物及西妥昔單抗(cetuximab)之頭頸癌組合療法
EP4452327A1 (en) 2021-12-20 2024-10-30 Synthorx, Inc. Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab
WO2023122750A1 (en) 2021-12-23 2023-06-29 Synthorx, Inc. Cancer combination therapy with il-2 conjugates and cetuximab
US12163190B2 (en) * 2022-06-17 2024-12-10 Insitro, Inc. In situ sequencing of RNA transcripts with non-uniform 5 prime ends
CN115028691B (zh) * 2022-06-29 2024-09-10 青岛硕景生物科技有限公司 一种检测试纸用的独立质控系统、杂交瘤细胞株、质控线用包被单克隆抗体及其应用
WO2024136899A1 (en) 2022-12-21 2024-06-27 Synthorx, Inc. Cancer therapy with il-2 conjugates and chimeric antigen receptor therapies
WO2024196937A1 (en) 2023-03-20 2024-09-26 Synthorx, Inc. Cancer therapy with il-2 peg conjugates
CN116925196B (zh) * 2023-06-08 2025-04-18 齐鲁工业大学(山东省科学院) 小分子热休克蛋白rsp_1572在提高宿主菌环境耐受性中的应用
WO2025158385A1 (en) 2024-01-25 2025-07-31 Genzyme Corporation Pegylated il-2 for suppressing adaptive immune response to gene therapy
CN118048325B (zh) * 2024-04-16 2024-07-16 天津凯莱英生物科技有限公司 酶的高通量筛选方法及酶库的构建方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003025116A2 (de) * 2001-09-17 2003-03-27 Roche Diagnostics Gmbh Verfahren zur erhöhung der löslichkeit, der expressionsrate und der aktivität von proteinen während der rekombinanten herstellung
CN101479379A (zh) * 2006-06-29 2009-07-08 利兰·斯坦福青年大学托管委员会 含有非天然氨基酸的蛋白质的无细胞合成
CN102348807A (zh) * 2009-01-12 2012-02-08 苏特罗生物制药公司 利用体外蛋白合成体系将非天然氨基酸引入蛋白的单装载体系
CN102732548A (zh) * 2012-05-16 2012-10-17 中国农业大学 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060134739A1 (en) 2003-03-11 2006-06-22 Chatterjee Deb K Synthesis of proteins by cell-free protein expression
JP2005253432A (ja) 2004-03-15 2005-09-22 Sekisui Chem Co Ltd タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット
JP4561243B2 (ja) 2004-08-27 2010-10-13 株式会社豊田中央研究所 無細胞タンパク質合成系のための媒体
US7871794B2 (en) * 2007-01-18 2011-01-18 The Board Of Trustees Of The Leland Stanford Junior University Enhanced cell-free synthesis of active proteins containing disulfide bonds
CA2887355C (en) * 2012-10-12 2023-03-07 Sutro Biopharma, Inc. Proteolytic inactivation of select proteins in bacterial extracts for improved expression
KR102289258B1 (ko) * 2013-04-19 2021-08-11 서트로 바이오파마, 인크. 상승된 수준의 외인성 샤페론을 갖는 세포 추출물을 이용한 박테리아 세포 비함유 합성 시스템에서의 생물학적 활성 단백질의 발현

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003025116A2 (de) * 2001-09-17 2003-03-27 Roche Diagnostics Gmbh Verfahren zur erhöhung der löslichkeit, der expressionsrate und der aktivität von proteinen während der rekombinanten herstellung
CN101479379A (zh) * 2006-06-29 2009-07-08 利兰·斯坦福青年大学托管委员会 含有非天然氨基酸的蛋白质的无细胞合成
CN102348807A (zh) * 2009-01-12 2012-02-08 苏特罗生物制药公司 利用体外蛋白合成体系将非天然氨基酸引入蛋白的单装载体系
CN102732548A (zh) * 2012-05-16 2012-10-17 中国农业大学 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Cell-Free Production of Aggregation-Prone Proteins in Soluble and Active Forms;Sang-Hyeon Kang等;《Biotechnology Progress》;20050508;第21卷(第5期);摘要，第1413页，表1 *
Cell-free production of functional antibody fragments;In-Seok Oh等;《Bioprocess and Biosystems Engineering》;20090823;第33卷(第1期);摘要，Materials and methods部分 *
Effect of folding factors in rescuing unstable heterologous lipase B to enhance its overexpression in the periplasm of Escherichia coli;Yali Xu等;《Applied Microbiology and Biotechnology》;20080522;第79卷(第6期);摘要，Materials and methods部分 *
Providing an Oxidizing Environment for the Cell-Free Expression of Disulfide-Containing Proteins by Exhausting the Reducing Activity of Escherichia coli S30 Extract;In-Seok Oh等;《Biotechnology Progress》;20060628;第22卷(第4期);摘要，Materials and Methods部分 *
SnapShot: Molecular Chaperones, Part II;Yun-Chi Tang等;《Cell》;20070126;第128卷(第2期);第212e1-212e2页 *

Also Published As

Publication number Publication date
EP2986731B1 (en) 2022-05-18
SG11201508642WA (en) 2015-11-27
EP2986731A2 (en) 2016-02-24
DK2986731T3 (da) 2022-08-01
AU2014253785B2 (en) 2018-07-19
US20140315245A1 (en) 2014-10-23
JP2016518833A (ja) 2016-06-30
HK1220993A1 (en) 2017-05-19
SG10202108497TA (en) 2021-09-29
CA2909516A1 (en) 2014-10-23
US20210054429A1 (en) 2021-02-25
KR102289258B1 (ko) 2021-08-11
WO2014172631A3 (en) 2014-12-18
JP2018157837A (ja) 2018-10-11
AU2014253785A1 (en) 2015-11-05
US10774354B2 (en) 2020-09-15
JP6463732B2 (ja) 2019-02-06
HUE059565T2 (hu) 2022-11-28
WO2014172631A4 (en) 2015-02-19
CA2909516C (en) 2023-03-07
KR20160002913A (ko) 2016-01-08
ES2922358T3 (es) 2022-09-13
IL242148B (en) 2019-03-31
CN105283556A (zh) 2016-01-27
US10190145B2 (en) 2019-01-29
US20190161781A1 (en) 2019-05-30
WO2014172631A2 (en) 2014-10-23

Similar Documents

Publication Publication Date Title
CN105283556B (zh) 在细菌无细胞合成系统中表达生物学活性蛋白
US11261219B2 (en) Proteolytic inactivation of select proteins in bacterial extracts for improved expression
US10487133B2 (en) Codon optimization for titer and fidelity improvement
CN101479379B (zh) 含有非天然氨基酸的蛋白质的无细胞合成
US20200332280A1 (en) Use of lambda-gam protein in ribosomal display technology
US20220089690A1 (en) Method for large scale production of antibodies using a cell-free protein synthesis system
HK1220993B (en) Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones
TWI899326B (zh) 使用無細胞蛋白質合成系統來大規模生產抗體之方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant