IL242148B - Expression of biologically active proteins in a cell-free bacterial expression system containing high levels of external chaperones - Google Patents
Expression of biologically active proteins in a cell-free bacterial expression system containing high levels of external chaperonesInfo
- Publication number
- IL242148B IL242148B IL242148A IL24214815A IL242148B IL 242148 B IL242148 B IL 242148B IL 242148 A IL242148 A IL 242148A IL 24214815 A IL24214815 A IL 24214815A IL 242148 B IL242148 B IL 242148B
- Authority
- IL
- Israel
- Prior art keywords
- expression
- biologically active
- elevated levels
- synthesis system
- active proteins
- Prior art date
Links
- 102000005431 Molecular Chaperones Human genes 0.000 title 1
- 108010006519 Molecular Chaperones Proteins 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 title 1
- 230000015572 biosynthetic process Effects 0.000 title 1
- 239000000284 extract Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361813914P | 2013-04-19 | 2013-04-19 | |
| US201461937069P | 2014-02-07 | 2014-02-07 | |
| PCT/US2014/034643 WO2014172631A2 (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using bacterial cells transformed to exhibit elevated levels of chaperone expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL242148B true IL242148B (en) | 2019-03-31 |
Family
ID=50733442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL242148A IL242148B (en) | 2013-04-19 | 2015-10-18 | Expression of biologically active proteins in a cell-free bacterial expression system containing high levels of external chaperones |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US10190145B2 (enExample) |
| EP (1) | EP2986731B1 (enExample) |
| JP (2) | JP6463732B2 (enExample) |
| KR (1) | KR102289258B1 (enExample) |
| CN (1) | CN105283556B (enExample) |
| AU (1) | AU2014253785B2 (enExample) |
| CA (1) | CA2909516C (enExample) |
| DK (1) | DK2986731T3 (enExample) |
| ES (1) | ES2922358T3 (enExample) |
| HU (1) | HUE059565T2 (enExample) |
| IL (1) | IL242148B (enExample) |
| SG (2) | SG10202108497TA (enExample) |
| WO (1) | WO2014172631A2 (enExample) |
Families Citing this family (57)
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| DK2986731T3 (da) | 2013-04-19 | 2022-08-01 | Sutro Biopharma Inc | Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner |
| WO2016004024A1 (en) | 2014-07-02 | 2016-01-07 | Sutro Biopharma, Inc. | High growth capacity auxotrophic escherichia coli and methods of use |
| RU2739500C2 (ru) * | 2014-11-05 | 2020-12-25 | Дженентек, Инк. | Способы получения двуцепочечных белков в бактериях |
| CN108064308B (zh) | 2014-11-05 | 2023-06-09 | 豪夫迈·罗氏有限公司 | 细菌中产生双链蛋白的方法 |
| WO2016100889A1 (en) * | 2014-12-19 | 2016-06-23 | Sutro Biopharma, Inc. | Codon optimization for titer and fidelity improvement |
| HK1245154A1 (zh) | 2014-12-30 | 2018-08-24 | 细胞基因公司 | 抗cd47抗体及其用途 |
| HRP20192217T1 (hr) * | 2015-03-06 | 2020-02-21 | F. Hoffmann - La Roche Ag | ULTRA-PROČIŠĆENI DsbA I DsbC TE POSTUPCI NJIHOVE IZRADE I UPORABE |
| US20170029798A1 (en) * | 2015-07-27 | 2017-02-02 | Cellivery Therapeutics, Inc. | Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD) |
| CN108473558A (zh) * | 2015-08-20 | 2018-08-31 | 豪夫迈·罗氏有限公司 | Fkpa的纯化及其用于生产重组多肽的用途 |
| CN105198983B (zh) * | 2015-09-11 | 2018-11-06 | 中国石油大学(华东) | 一种利于七次跨膜蛋白ccr5功能性表达的方法 |
| CN106190940B (zh) * | 2016-07-19 | 2019-06-14 | 北京三元基因药业股份有限公司 | 表达抗TNF抗体Fab片段的重组大肠杆菌工程菌 |
| CN106399352B (zh) * | 2016-11-09 | 2020-04-17 | 华东理工大学 | 调节目标蛋白表达的折叠因子及其应用 |
| US11136586B2 (en) | 2016-12-30 | 2021-10-05 | Ntxbio, Llc | Cell-free expression system having novel inorganic polyphosphate-based energy regeneration |
| IL267709B2 (en) | 2016-12-30 | 2025-04-01 | Vaxcyte Inc | Polypeptide-antigen conjugates with unnatural amino acids |
| US12227778B2 (en) | 2016-12-30 | 2025-02-18 | Nature's Toolbox, Inc. | Cell-free expression system having novel inorganic polyphosphate-based energy regeneration |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| WO2019014398A1 (en) | 2017-07-11 | 2019-01-17 | Actym Therapeutics, Inc. | MODIFIED IMMUNOSTIMULATORY BACTERIAL STRAINS AND USES THEREOF |
| TWI821192B (zh) | 2017-07-11 | 2023-11-11 | 美商新索思股份有限公司 | 非天然核苷酸之導入及其方法 |
| IL321812A (en) | 2017-08-03 | 2025-08-01 | Synthorx Inc | Cytokine complexes for the treatment of autoimmune diseases |
| EP3665188A4 (en) * | 2017-08-11 | 2021-07-21 | Synvitrobio, Inc. | IMPROVED IN VITRO TRANSLATION / TRANSLATION SYSTEM (TXTL) AND ITS USES |
| WO2019127469A1 (zh) * | 2017-12-29 | 2019-07-04 | 康码（上海）生物科技有限公司 | 一种高效的能源再生体系(bes)、试剂盒及其制备方法 |
| AU2019224159B2 (en) | 2018-02-26 | 2025-05-29 | Sanofi | IL-15 conjugates and uses thereof |
| CA3176812A1 (en) | 2018-07-11 | 2020-01-16 | Actym Therapeutics, Inc. | Engineered immunostimulatory bacterial strains and uses thereof |
| CN113260626B (zh) | 2018-11-05 | 2024-09-13 | 豪夫迈·罗氏有限公司 | 在原核宿主细胞中产生双链蛋白质的方法 |
| TW202031284A (zh) | 2018-11-08 | 2020-09-01 | 美商新索思股份有限公司 | 介白素10結合物及其用途 |
| IL282875B2 (en) * | 2018-11-08 | 2026-05-01 | Sutro Biopharma Inc | E. coli strains with oxygenated cytoplasm |
| CN109371049A (zh) * | 2018-11-14 | 2019-02-22 | 天津大学 | 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用 |
| MA54952A (fr) | 2019-02-06 | 2022-05-11 | Synthorx Inc | Conjugués d'il-2 et méthodes d'utilisation de ceux-ci |
| KR20220004959A (ko) | 2019-02-27 | 2022-01-12 | 액팀 테라퓨틱스, 인코퍼레이티드 | 종양, 종양-상주 면역 세포, 및 종양 미세환경을 콜로니화하기 위해 조작된 면역자극성 박테리아 |
| EP4013454A1 (en) | 2019-08-15 | 2022-06-22 | Synthorx, Inc. | Immuno oncology combination therapies with il-2 conjugates |
| WO2021050554A1 (en) | 2019-09-10 | 2021-03-18 | Synthorx, Inc. | Il-2 conjugates and methods of use to treat autoimmune diseases |
| JP2022554272A (ja) | 2019-11-04 | 2022-12-28 | シンソークス， インコーポレイテッド | インターロイキン１０コンジュゲートおよびその使用 |
| US12679875B2 (en) * | 2019-11-08 | 2026-07-14 | Korea Institute Of Ocean Science & Technology | Human epidermal growth factor-TF fusion protein and use thereof |
| EP4114931A4 (en) * | 2020-03-05 | 2024-05-01 | Curie Co. Inc. | METHODS FOR CELL PROTEIN EXPRESSION OF MATURE POLYPEPTIDES DERIVED FROM ZYMOGENS AND PROPROTEINS |
| EP4142711A1 (en) * | 2020-04-29 | 2023-03-08 | Willow Biosciences Inc. | Compositions and methods for enhancing recombinant biosynthesis of cannabinoids |
| WO2021263026A1 (en) | 2020-06-25 | 2021-12-30 | Synthorx, Inc. | Immuno oncology combination therapy with il-2 conjugates and anti-egfr antibodies |
| KR20220000028U (ko) | 2020-06-29 | 2022-01-05 | 채인자 | 농작물용 농약 간편 분무장치 |
| EP4211156A1 (en) * | 2020-09-14 | 2023-07-19 | Sutro Biopharma, Inc. | Method for large scale production of antibodies using a cell-free protein synthesis system |
| EP4225376A1 (en) | 2020-10-09 | 2023-08-16 | Synthorx, Inc. | Immuno oncology combination therapy with il-2 conjugates and pembrolizumab |
| IL301612A (en) | 2020-10-09 | 2023-05-01 | Synthorx Inc | Immuno oncology therapies with il-2 conjugates |
| WO2022104367A1 (en) | 2020-11-12 | 2022-05-19 | Northwestern University | Cell-free expression of antibodies, antigen-binding fragments thereof, and antibody derivatives |
| TW202302148A (zh) | 2021-02-12 | 2023-01-16 | 美商欣爍克斯公司 | 使用ｉｌ－２接合物和抗ｐｄ－１抗體或其抗原結合片段的肺癌組合療法 |
| WO2022174101A1 (en) | 2021-02-12 | 2022-08-18 | Synthorx, Inc. | Skin cancer combination therapy with il-2 conjugates and cemiplimab |
| WO2022256534A1 (en) | 2021-06-03 | 2022-12-08 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| EP4452327A1 (en) | 2021-12-20 | 2024-10-30 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122750A1 (en) | 2021-12-23 | 2023-06-29 | Synthorx, Inc. | Cancer combination therapy with il-2 conjugates and cetuximab |
| CN116555061B (zh) * | 2022-01-28 | 2026-07-17 | 北京师范大学 | 一种酿酒酵母突变体及其在制备酒精中的应用 |
| US12163190B2 (en) * | 2022-06-17 | 2024-12-10 | Insitro, Inc. | In situ sequencing of RNA transcripts with non-uniform 5 prime ends |
| CN115028691B (zh) * | 2022-06-29 | 2024-09-10 | 青岛硕景生物科技有限公司 | 一种检测试纸用的独立质控系统、杂交瘤细胞株、质控线用包被单克隆抗体及其应用 |
| WO2024136899A1 (en) | 2022-12-21 | 2024-06-27 | Synthorx, Inc. | Cancer therapy with il-2 conjugates and chimeric antigen receptor therapies |
| CN121263208A (zh) | 2023-03-20 | 2026-01-02 | 新索思股份有限公司 | 使用il-2 peg缀合物的癌症疗法 |
| CN116925196B (zh) * | 2023-06-08 | 2025-04-18 | 齐鲁工业大学(山东省科学院) | 小分子热休克蛋白rsp_1572在提高宿主菌环境耐受性中的应用 |
| WO2025158385A1 (en) | 2024-01-25 | 2025-07-31 | Genzyme Corporation | Pegylated il-2 for suppressing adaptive immune response to gene therapy |
| CN118048325B (zh) * | 2024-04-16 | 2024-07-16 | 天津凯莱英生物科技有限公司 | 酶的高通量筛选方法及酶库的构建方法 |
| WO2025218039A1 (zh) * | 2024-04-16 | 2025-10-23 | 天津凯莱英生物科技有限公司 | 酶的高通量筛选方法及酶库的构建方法 |
| WO2025240255A1 (en) * | 2024-05-11 | 2025-11-20 | Biodiscovery, Llc | Systems and methods of cell-free protein expression |
| WO2026136953A2 (en) | 2024-12-20 | 2026-06-25 | Sutro Biopharma, Inc. | High cell density fed-batch fermentation |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10145694A1 (de) | 2001-09-17 | 2003-04-03 | Roche Diagnostics Gmbh | Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung |
| US20060134739A1 (en) * | 2003-03-11 | 2006-06-22 | Chatterjee Deb K | Synthesis of proteins by cell-free protein expression |
| JP2005253432A (ja) * | 2004-03-15 | 2005-09-22 | Sekisui Chem Co Ltd | タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット |
| JP4561243B2 (ja) * | 2004-08-27 | 2010-10-13 | 株式会社豊田中央研究所 | 無細胞タンパク質合成系のための媒体 |
| DK2035554T3 (da) * | 2006-06-29 | 2013-06-17 | Univ Leland Stanford Junior | Celle-fri syntese af proteiner indeholdende ikke-naturlige aminosyrer |
| CA2673765A1 (en) * | 2007-01-18 | 2008-07-24 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced cell-free synthesis of active proteins containing disulfide bonds |
| EP2379578A4 (en) | 2009-01-12 | 2012-05-02 | Sutro Biopharma Inc | DOUBLE-LOADING SYSTEM FOR THE SELECTIVE INTRODUCTION OF NON-NITIVE AMINO ACIDS IN PROTEINS DUE TO AN IN VITRO-SYNTHESIS PROCESS |
| US20110136169A1 (en) * | 2009-11-20 | 2011-06-09 | Rutgers, The State University Of New Jersey | Expression system compositions and methods |
| CN102732548A (zh) | 2012-05-16 | 2012-10-17 | 中国农业大学 | 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用 |
| WO2014058830A1 (en) * | 2012-10-12 | 2014-04-17 | Sutro Biopharma, Inc. | Proteolytic inactivation of select proteins in bacterial extracts for improved expression |
| DK2986731T3 (da) | 2013-04-19 | 2022-08-01 | Sutro Biopharma Inc | Ekspression af biologisk aktive proteiner i et bakteriecellefrit syntesesystem ved hjælp af celleekstrakter med forhøjede niveauer af exogene chaperoner |
-
2014
- 2014-04-18 DK DK14724974.2T patent/DK2986731T3/da active
- 2014-04-18 WO PCT/US2014/034643 patent/WO2014172631A2/en not_active Ceased
- 2014-04-18 JP JP2016509127A patent/JP6463732B2/ja active Active
- 2014-04-18 CA CA2909516A patent/CA2909516C/en active Active
- 2014-04-18 KR KR1020157032515A patent/KR102289258B1/ko active Active
- 2014-04-18 EP EP14724974.2A patent/EP2986731B1/en active Active
- 2014-04-18 AU AU2014253785A patent/AU2014253785B2/en active Active
- 2014-04-18 CN CN201480032258.8A patent/CN105283556B/zh active Active
- 2014-04-18 HU HUE14724974A patent/HUE059565T2/hu unknown
- 2014-04-18 SG SG10202108497TA patent/SG10202108497TA/en unknown
- 2014-04-18 ES ES14724974T patent/ES2922358T3/es active Active
- 2014-04-18 SG SG11201508642WA patent/SG11201508642WA/en unknown
- 2014-04-18 US US14/256,324 patent/US10190145B2/en active Active
-
2015
- 2015-10-18 IL IL242148A patent/IL242148B/en active IP Right Grant
-
2018
- 2018-07-20 JP JP2018136909A patent/JP2018157837A/ja not_active Withdrawn
- 2018-12-03 US US16/208,245 patent/US10774354B2/en active Active
-
2020
- 2020-08-12 US US16/991,607 patent/US20210054429A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20160002913A (ko) | 2016-01-08 |
| JP2018157837A (ja) | 2018-10-11 |
| ES2922358T3 (es) | 2022-09-13 |
| US20190161781A1 (en) | 2019-05-30 |
| CN105283556B (zh) | 2020-07-14 |
| US20210054429A1 (en) | 2021-02-25 |
| AU2014253785B2 (en) | 2018-07-19 |
| SG11201508642WA (en) | 2015-11-27 |
| KR102289258B1 (ko) | 2021-08-11 |
| WO2014172631A4 (en) | 2015-02-19 |
| HUE059565T2 (hu) | 2022-11-28 |
| US10190145B2 (en) | 2019-01-29 |
| US20140315245A1 (en) | 2014-10-23 |
| US10774354B2 (en) | 2020-09-15 |
| DK2986731T3 (da) | 2022-08-01 |
| JP2016518833A (ja) | 2016-06-30 |
| CA2909516A1 (en) | 2014-10-23 |
| WO2014172631A3 (en) | 2014-12-18 |
| SG10202108497TA (en) | 2021-09-29 |
| EP2986731B1 (en) | 2022-05-18 |
| JP6463732B2 (ja) | 2019-02-06 |
| CA2909516C (en) | 2023-03-07 |
| WO2014172631A2 (en) | 2014-10-23 |
| CN105283556A (zh) | 2016-01-27 |
| EP2986731A2 (en) | 2016-02-24 |
| HK1220993A1 (en) | 2017-05-19 |
| AU2014253785A1 (en) | 2015-11-05 |
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Legal Events
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| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed |