DK2035554T3 - Celle-fri syntese af proteiner indeholdende ikke-naturlige aminosyrer - Google Patents

Celle-fri syntese af proteiner indeholdende ikke-naturlige aminosyrer

Info

Publication number
DK2035554T3
DK2035554T3 DK07870711.4T DK07870711T DK2035554T3 DK 2035554 T3 DK2035554 T3 DK 2035554T3 DK 07870711 T DK07870711 T DK 07870711T DK 2035554 T3 DK2035554 T3 DK 2035554T3
Authority
DK
Denmark
Prior art keywords
cell
amino acids
natural amino
containing non
proteins containing
Prior art date
Application number
DK07870711.4T
Other languages
English (en)
Inventor
Aaron R Goerke
James Robert Swartz
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Application granted granted Critical
Publication of DK2035554T3 publication Critical patent/DK2035554T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK07870711.4T 2006-06-29 2007-06-29 Celle-fri syntese af proteiner indeholdende ikke-naturlige aminosyrer DK2035554T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US81791506P 2006-06-29 2006-06-29
US81777206P 2006-06-29 2006-06-29
PCT/US2007/015170 WO2008066583A2 (en) 2006-06-29 2007-06-29 Cell-free synthesis of proteins containing unnatural amino acids

Publications (1)

Publication Number Publication Date
DK2035554T3 true DK2035554T3 (da) 2013-06-17

Family

ID=39468405

Family Applications (1)

Application Number Title Priority Date Filing Date
DK07870711.4T DK2035554T3 (da) 2006-06-29 2007-06-29 Celle-fri syntese af proteiner indeholdende ikke-naturlige aminosyrer

Country Status (9)

Country Link
US (1) US8715958B2 (da)
EP (1) EP2035554B1 (da)
JP (1) JP5620100B2 (da)
CN (1) CN101479379B (da)
AU (1) AU2007325952B8 (da)
CA (1) CA2657811C (da)
DK (1) DK2035554T3 (da)
ES (1) ES2415635T3 (da)
WO (1) WO2008066583A2 (da)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100063258A1 (en) * 2006-06-28 2010-03-11 The Board Of Trustees Of The Leland Stanford Junior University Fusion protein constructs
EP2889370A1 (en) 2007-12-11 2015-07-01 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris
JP5959201B2 (ja) * 2008-12-10 2016-08-02 ザ スクリプス リサーチ インスティテュート 化学的に反応性の非天然アミノ酸を使用するキャリア−ペプチド接合体の製造
US9637746B2 (en) 2008-12-15 2017-05-02 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways
JP2013526277A (ja) 2010-05-07 2013-06-24 グリーンライト バイオサイエンシーズ インコーポレーテッド 酵素リロケーションにより代謝経路のフラックスを制御する方法
KR101988063B1 (ko) 2010-08-31 2019-09-30 그린라이트 바이오사이언시스, 아이엔씨. 프로테아제 조작을 통한 대사 경로에서 유동의 제어 방법
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
US12227734B2 (en) 2011-03-08 2025-02-18 University Of Maryland, Baltimore County Microscale bioprocessing system and method for protein manufacturing from human blood
US9982227B2 (en) * 2011-03-08 2018-05-29 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
US10774304B2 (en) 2011-03-08 2020-09-15 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
EP2753702B1 (en) 2011-09-09 2021-12-15 GreenLight Biosciences, Inc. Cell-free preparation of carbapenems
US9637522B2 (en) 2012-05-23 2017-05-02 The Board Of Trustees Of The Leland Stanford Junior University Influenza vaccine constructs
ES2718478T3 (es) 2012-06-08 2019-07-02 Sutro Biopharma Inc Anticuerpos que comprenden restos de aminoácidos no naturales de localización específica, métodos para su preparación y métodos para su uso
DK2863955T3 (da) 2012-06-26 2017-01-23 Sutro Biopharma Inc Modificerede fc-proteiner, der omfatter stedsspecifikke ikke-naturlige aminosyrerester, konjugater heraf, fremgangsmåder til fremstilling heraf og fremgangsmåder til anvendelse heraf
SG11201501464TA (en) 2012-08-31 2015-03-30 Sutro Biopharma Inc Modified amino acids comprising an azido group
WO2014100722A1 (en) 2012-12-21 2014-06-26 Greenlight Biosciences, Inc. Cell-free system for converting methane into fuel, pyruvate or isobutanol
JP2016507520A (ja) 2013-01-23 2016-03-10 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 安定化されたb型肝炎コアポリペプチド
SI2956173T1 (sl) 2013-02-14 2017-06-30 Bristol-Myers Squibb Company Spojine tubulizina, postopki pridobivanja in uporaba
SG10202108497TA (en) * 2013-04-19 2021-09-29 Sutro Biopharma Inc Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones
CA2913999A1 (en) * 2013-06-05 2014-12-11 Greenlight Biosciences, Inc. Control of metabolic flux in cell-free biosynthetic systems
US9764039B2 (en) 2013-07-10 2017-09-19 Sutro Biopharma, Inc. Antibodies comprising multiple site-specific non-natural amino acid residues, methods of their preparation and methods of their use
JP6332585B2 (ja) * 2013-07-11 2018-05-30 国立研究開発法人農業・食品産業技術総合研究機構 トランスジェニックカイコを用いた非天然アミノ酸含有タンパク質の製造方法
US9688977B2 (en) 2013-08-05 2017-06-27 Greenlight Biosciences, Inc. Engineered phosphoglucose isomerase proteins with a protease cleavage site
MX368016B (es) 2013-08-14 2019-09-13 Univ Rice William M Derivados de uncialamicina, métodos de sintesis y su uso como agentes antitumor.
WO2015054658A1 (en) 2013-10-11 2015-04-16 Sutro Biopharma, Inc. Modified amino acids comprising tetrazine functional groups, methods of preparation, and methods of their use
EP3310924A1 (en) * 2014-06-17 2018-04-25 B.G. Negev Technologies and Applications Ltd., at Ben-Gurion University Genetically expanded cell free protein synthesis systems, methods and kits
US10316322B2 (en) 2014-07-02 2019-06-11 Sutro Biopharma, Inc. High growth capacity auxotrophic Escherichia coli and methods of use
WO2016044328A1 (en) * 2014-09-18 2016-03-24 The Regents Of The University Of California Single-molecule phenotype analysis
US10077287B2 (en) 2014-11-10 2018-09-18 Bristol-Myers Squibb Company Tubulysin analogs and methods of making and use
ES2986970T3 (es) 2014-12-04 2024-11-13 Celgene Corp Conjugados de biomoléculas
ES2747386T3 (es) 2015-01-14 2020-03-10 Bristol Myers Squibb Co Dímeros de benzodiacepina unidos por heteroarileno, conjugados de los mismos y métodos de preparación y uso
RU2017137788A (ru) 2015-03-30 2019-04-30 Гринлайт Байосайенсис, Инк. Бесклеточное получение рибонуклеиновой кислоты
JP2019505575A (ja) 2015-12-21 2019-02-28 ブリストル−マイヤーズ スクイブ カンパニーBristol−Myers Squibb Company 部位特異的な結合のための変異型抗体
SG11201806419RA (en) 2016-01-27 2018-08-30 Sutro Biopharma Inc Anti-cd74 antibody conjugates, compositions comprising anti-cd74 antibody conjugates and methods of using anti-cd74 antibody conjugates
SG11201808721YA (en) 2016-04-06 2018-11-29 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
ES2902179T3 (es) 2016-08-19 2022-03-25 Bristol Myers Squibb Co Compuestos de seco-ciclopropapirroloindol, conjugados de anticuerpo-fármaco de los mismos y métodos de elaboración y uso
WO2018075842A1 (en) 2016-10-20 2018-04-26 Bristol-Myers Squibb Company Condensed benzodiazepine derivatives and conjugates made therefrom
DK3562503T3 (da) 2016-12-30 2025-05-26 Vaxcyte Inc Polypeptid-antigen-konjugater med ikke-naturlige aminosyrer
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
WO2018129275A1 (en) 2017-01-06 2018-07-12 Greenlight Biosciences, Inc. Cell-free production of sugars
CN108396034A (zh) * 2017-02-06 2018-08-14 武汉臻智生物科技有限公司 一种提高无细胞体系蛋白质合成的方法
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US10487084B2 (en) 2017-08-16 2019-11-26 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a heterobiaryl moiety, conjugates thereof, and methods and uses therefor
US10494370B2 (en) 2017-08-16 2019-12-03 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a pyridine or pyrazine moiety, conjugates thereof, and methods and uses therefor
US10472361B2 (en) 2017-08-16 2019-11-12 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a benzotriazole moiety, conjugates thereof, and methods and uses therefor
US10508115B2 (en) 2017-08-16 2019-12-17 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having heteroatom-linked aromatic moieties, conjugates thereof, and methods and uses therefor
US10457681B2 (en) 2017-08-16 2019-10-29 Bristol_Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a tricyclic moiety, conjugates thereof, and methods and uses therefor
JP7792196B2 (ja) 2017-10-11 2025-12-25 グリーンライト バイオサイエンシーズ インコーポレーテッド ヌクレオシド三リン酸およびリボ核酸の生成のための方法および組成物
CN112739345A (zh) 2017-11-06 2021-04-30 斯奈普生物公司 Pim激酶抑制剂组合物,方法和其用途
WO2019204346A1 (en) 2018-04-16 2019-10-24 Northwestern University METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES
WO2019209811A1 (en) 2018-04-24 2019-10-31 Bristol-Myers Squibb Company Macrocyclic toll-like receptor 7 (tlr7) agonists
AU2019277094B2 (en) 2018-05-29 2025-12-18 Bristol-Myers Squibb Company Modified self-immolating moieties for use in prodrugs and conjugates and methods of using and making
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
WO2020010016A1 (en) 2018-07-04 2020-01-09 Sutrovax, Inc. Self-adjuvanted immunogenic conjugates
US12421537B2 (en) 2018-07-06 2025-09-23 Northwestern University Ribosome variants for sequence defined polymer synthesis
WO2020072127A2 (en) 2018-08-03 2020-04-09 Northwestern University On demand, portable, cell-free molecular sensing platform
US11554120B2 (en) 2018-08-03 2023-01-17 Bristol-Myers Squibb Company 1H-pyrazolo[4,3-d]pyrimidine compounds as toll-like receptor 7 (TLR7) agonists and methods and uses therefor
GB201817932D0 (en) * 2018-11-02 2018-12-19 Origin Ip Peptide synthesis
EP4108672B1 (en) * 2020-02-17 2025-08-13 Yichen Therapeutics Limited Antibody screening method
US20230095053A1 (en) 2020-03-03 2023-03-30 Sutro Biopharma, Inc. Antibodies comprising site-specific glutamine tags, methods of their preparation and methods of their use
TWI899326B (zh) 2020-09-14 2025-10-01 美商蘇特羅生物製藥公司 使用無細胞蛋白質合成系統來大規模生產抗體之方法
CA3213076A1 (en) * 2021-03-24 2022-09-29 Danielle Jessica Yoesep Systems and methods for cell-free polypeptide synthesis
CN114634958B (zh) * 2021-12-22 2024-08-16 清华大学 使用无细胞蛋白质合成体系插入非天然氨基酸的方法
TW202424204A (zh) 2022-08-08 2024-06-16 美商蘇特羅生物製藥公司 噴霧乾燥之萃取物
CN120380161A (zh) * 2022-12-31 2025-07-25 康码(上海)生物科技有限公司 一种用于插入非天然氨基酸的体外无细胞蛋白质合成体系、方法及应用
WO2025091009A2 (en) 2023-10-27 2025-05-01 Sutro Biopharma, Inc. Overexpression of lysozyme in t7 rna polymerase-expressing host cells
WO2025188694A1 (en) 2024-03-05 2025-09-12 Bristol-Myers Squibb Company Tricyclic tlr7 agonists and uses thereof
US20250282776A1 (en) 2024-03-05 2025-09-11 Bristol-Myers Squibb Company Bicyclic TLR7 Agonists and Uses Thereof
WO2026043823A2 (en) 2024-08-19 2026-02-26 Sutro Biopharma, Inc. Antibodies comprising site-specific non-natural amino acid residues, methods of preparation and uses thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6337191B1 (en) 1999-03-22 2002-01-08 The Board Of Trustees Of The Leland Stanford Junior University Vitro protein synthesis using glycolytic intermediates as an energy source
DK2322631T3 (da) * 2001-04-19 2015-01-12 Scripps Research Inst Fremgangsmåder og sammensætninger til fremstilling af ortogonale tRNA-aminoacyl-tRNA-syntetasepar
AU2003259912B2 (en) 2002-08-19 2008-10-23 The Board Of Trustees Of The Leland Stanford Junior University Improved methods of in vitro protein synthesis
JP3896460B2 (ja) 2003-03-04 2007-03-22 国立大学法人岐阜大学 非天然蛋白質の製造方法、固定化方法及びキット
US7341852B2 (en) 2003-07-18 2008-03-11 The Board Of Trustees Of The Leland Stanford Junior University Methods of decoupling reaction scale and protein synthesis yield in batch mode
US20050054044A1 (en) 2003-07-18 2005-03-10 The Board Of Trustees Of The Leland Stanford Junior University Method of alleviating nucleotide limitations for in vitro protein synthesis
US9410170B2 (en) 2003-11-20 2016-08-09 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vitro protein synthesis
WO2007130453A2 (en) * 2006-05-02 2007-11-15 Allozyne, Inc. Non-natural amino acid substituted polypeptides

Also Published As

Publication number Publication date
JP5620100B2 (ja) 2014-11-05
CN101479379B (zh) 2012-01-18
AU2007325952A1 (en) 2008-06-05
EP2035554A2 (en) 2009-03-18
US8715958B2 (en) 2014-05-06
CA2657811A1 (en) 2008-06-05
CN101479379A (zh) 2009-07-08
EP2035554A4 (en) 2012-02-22
WO2008066583A9 (en) 2008-07-24
AU2007325952B8 (en) 2013-06-27
US20100093024A1 (en) 2010-04-15
CA2657811C (en) 2014-10-21
EP2035554B1 (en) 2013-04-24
WO2008066583A2 (en) 2008-06-05
AU2007325952A8 (en) 2013-06-27
AU2007325952B2 (en) 2008-06-05
AU2007325952A2 (en) 2009-05-14
WO2008066583A3 (en) 2008-12-18
ES2415635T3 (es) 2013-07-26
JP2009542214A (ja) 2009-12-03

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