DK2035554T3 - Celle-fri syntese af proteiner indeholdende ikke-naturlige aminosyrer - Google Patents

Celle-fri syntese af proteiner indeholdende ikke-naturlige aminosyrer

Info

Publication number
DK2035554T3
DK2035554T3 DK07870711.4T DK07870711T DK2035554T3 DK 2035554 T3 DK2035554 T3 DK 2035554T3 DK 07870711 T DK07870711 T DK 07870711T DK 2035554 T3 DK2035554 T3 DK 2035554T3
Authority
DK
Denmark
Prior art keywords
cell
amino acids
natural amino
containing non
proteins containing
Prior art date
Application number
DK07870711.4T
Other languages
English (en)
Inventor
Aaron R Goerke
James Robert Swartz
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Application granted granted Critical
Publication of DK2035554T3 publication Critical patent/DK2035554T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
DK07870711.4T 2006-06-29 2007-06-29 Celle-fri syntese af proteiner indeholdende ikke-naturlige aminosyrer DK2035554T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US81777206P 2006-06-29 2006-06-29
US81791506P 2006-06-29 2006-06-29
PCT/US2007/015170 WO2008066583A2 (en) 2006-06-29 2007-06-29 Cell-free synthesis of proteins containing unnatural amino acids

Publications (1)

Publication Number Publication Date
DK2035554T3 true DK2035554T3 (da) 2013-06-17

Family

ID=39468405

Family Applications (1)

Application Number Title Priority Date Filing Date
DK07870711.4T DK2035554T3 (da) 2006-06-29 2007-06-29 Celle-fri syntese af proteiner indeholdende ikke-naturlige aminosyrer

Country Status (9)

Country Link
US (1) US8715958B2 (da)
EP (1) EP2035554B1 (da)
JP (1) JP5620100B2 (da)
CN (1) CN101479379B (da)
AU (1) AU2007325952B8 (da)
CA (1) CA2657811C (da)
DK (1) DK2035554T3 (da)
ES (1) ES2415635T3 (da)
WO (1) WO2008066583A2 (da)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100063258A1 (en) * 2006-06-28 2010-03-11 The Board Of Trustees Of The Leland Stanford Junior University Fusion protein constructs
BRPI0819929A2 (pt) 2007-12-11 2014-10-14 Scripps Research Inst Expressão de amino ácido não natural em vivo na levedura pichia pastoris metilotrófica
WO2010068278A2 (en) 2008-12-10 2010-06-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US9637746B2 (en) 2008-12-15 2017-05-02 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways
EP2566953B1 (en) 2010-05-07 2019-01-02 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through enzyme relocation
EP3219796B1 (en) 2010-08-31 2020-10-07 GreenLight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
US9982227B2 (en) 2011-03-08 2018-05-29 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
US10774304B2 (en) 2011-03-08 2020-09-15 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
CN104093848A (zh) 2011-09-09 2014-10-08 绿光生物科学公司 碳青霉烯(carbapenem)的无细胞制备
CN104582714A (zh) 2012-05-23 2015-04-29 斯坦福大学托管董事会 流感疫苗构建体
US9738724B2 (en) 2012-06-08 2017-08-22 Sutro Biopharma, Inc. Antibodies comprising site-specific non-natural amino acid residues, methods of their preparation and methods of their use
WO2014004639A1 (en) 2012-06-26 2014-01-03 Sutro Biopharma, Inc. Modified fc proteins comprising site-specific non-natural amino acid residues, conjugates of the same, methods of their preparation and methods of their use
WO2014036492A1 (en) 2012-08-31 2014-03-06 Sutro Biopharma, Inc. Modified amino acids comprising an azido group
MX2015008188A (es) 2012-12-21 2016-02-05 Greenlight Biosciences Inc Sistema libre de celulas para convertir metano en combustible, piruvato o isobutanol.
JP2016507520A (ja) 2013-01-23 2016-03-10 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 安定化されたb型肝炎コアポリペプチド
SG11201506243XA (en) 2013-02-14 2015-09-29 Bristol Myers Squibb Co Tubulysin compounds, methods of making and use
SG10202108497TA (en) * 2013-04-19 2021-09-29 Sutro Biopharma Inc Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones
US20160115558A1 (en) * 2013-06-05 2016-04-28 Greenlight Biosciences, Inc. Control of metabolic flux in cell-free biosynthetic systems
WO2015006555A2 (en) 2013-07-10 2015-01-15 Sutro Biopharma, Inc. Antibodies comprising multiple site-specific non-natural amino acid residues, methods of their preparation and methods of their use
JP6332585B2 (ja) * 2013-07-11 2018-05-30 国立研究開発法人農業・食品産業技術総合研究機構 トランスジェニックカイコを用いた非天然アミノ酸含有タンパク質の製造方法
JP6483687B2 (ja) 2013-08-05 2019-03-13 グリーンライト バイオサイエンシーズ インコーポレーテッドGreenlight Biosciences,Inc. プロテアーゼ切断部位を有する操作されたタンパク質
AU2014306592B2 (en) 2013-08-14 2019-04-04 Bristol-Myers Squibb Company Derivatives of uncialamycin, methods of synthesis and their use as antitumor agents
WO2015054658A1 (en) 2013-10-11 2015-04-16 Sutro Biopharma, Inc. Modified amino acids comprising tetrazine functional groups, methods of preparation, and methods of their use
US20170292139A1 (en) * 2014-06-17 2017-10-12 B.G NEGEV TECHNOLOGIES AND APPLICATIONS LTD., at BEN-GURION UNIVERSITY Genetically expanded cell free protein synthesis systems, methods and kits
EP3164501A4 (en) 2014-07-02 2018-01-17 Sutro Biopharma, Inc. High growth capacity auxotrophic escherichia coli and methods of use
US11634706B2 (en) 2014-09-18 2023-04-25 The Trustees of the California State University Single-molecule phenotype analysis
US10077287B2 (en) 2014-11-10 2018-09-18 Bristol-Myers Squibb Company Tubulysin analogs and methods of making and use
JP2018502067A (ja) 2014-12-04 2018-01-25 セルジーン コーポレイション 生体分子複合体
CA2973355A1 (en) 2015-01-14 2016-07-21 Bristol-Myers Squibb Company Heteroarylene-bridged benzodiazepine dimers, conjugates thereof, and methods of making and using
CN107614682B (zh) 2015-03-30 2021-11-09 绿光生物科技股份有限公司 核糖核酸的无细胞生成
WO2017112624A1 (en) 2015-12-21 2017-06-29 Bristol-Myers Squibb Company Variant antibodies for site-specific conjugation
RU2018130757A (ru) 2016-01-27 2020-02-27 Сутро Биофарма, Инк. Конъюгаты антитела к cd74, композиции, содержащие конъюгаты антитела к cd74, и способы использования конъюгатов антитела к cd74
WO2017176963A1 (en) 2016-04-06 2017-10-12 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
US10287291B2 (en) 2016-08-19 2019-05-14 Bristol-Myers Squibb Company Seco-cyclopropapyrroloindole compounds, antibody-drug conjugates thereof, and methods of making and use
US10398783B2 (en) 2016-10-20 2019-09-03 Bristol-Myers Squibb Company Antiproliferative compounds and conjugates made therefrom
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
WO2018126229A2 (en) 2016-12-30 2018-07-05 Sutrovax, Inc. Polypeptide-antigen conjugates with non-natural amino acids
MX2019008159A (es) 2017-01-06 2019-12-09 Greenlight Biosciences Inc Producción acelular de azúcares.
CN108396034A (zh) * 2017-02-06 2018-08-14 武汉臻智生物科技有限公司 一种提高无细胞体系蛋白质合成的方法
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US10472361B2 (en) 2017-08-16 2019-11-12 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a benzotriazole moiety, conjugates thereof, and methods and uses therefor
US10494370B2 (en) 2017-08-16 2019-12-03 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a pyridine or pyrazine moiety, conjugates thereof, and methods and uses therefor
US10487084B2 (en) 2017-08-16 2019-11-26 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a heterobiaryl moiety, conjugates thereof, and methods and uses therefor
US10508115B2 (en) 2017-08-16 2019-12-17 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having heteroatom-linked aromatic moieties, conjugates thereof, and methods and uses therefor
US10457681B2 (en) 2017-08-16 2019-10-29 Bristol_Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a tricyclic moiety, conjugates thereof, and methods and uses therefor
SG11202003192UA (en) 2017-10-11 2020-05-28 Greenlight Biosciences Inc Methods and compositions for nucleoside triphosphate and ribonucleic acid production
EP3706735A1 (en) 2017-11-06 2020-09-16 Snap Bio, Inc. Pim kinase inhibitor compositions, methods, and uses thereof
WO2019204346A1 (en) 2018-04-16 2019-10-24 Northwestern University METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES
WO2019209811A1 (en) 2018-04-24 2019-10-31 Bristol-Myers Squibb Company Macrocyclic toll-like receptor 7 (tlr7) agonists
MX2020012674A (es) 2018-05-29 2021-02-09 Bristol Myers Squibb Co Porciones autoinmolantes modificadas para usarse en profarmacos y conjugados y metodos de uso y fabricacion.
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
WO2020010016A1 (en) 2018-07-04 2020-01-09 Sutrovax, Inc. Self-adjuvanted immunogenic conjugates
US11554120B2 (en) 2018-08-03 2023-01-17 Bristol-Myers Squibb Company 1H-pyrazolo[4,3-d]pyrimidine compounds as toll-like receptor 7 (TLR7) agonists and methods and uses therefor
WO2021178597A1 (en) 2020-03-03 2021-09-10 Sutro Biopharma, Inc. Antibodies comprising site-specific glutamine tags, methods of their preparation and methods of their use
EP4211156A1 (en) 2020-09-14 2023-07-19 Sutro Biopharma, Inc. Method for large scale production of antibodies using a cell-free protein synthesis system
CN114634958A (zh) * 2021-12-22 2022-06-17 清华大学 使用无细胞蛋白质合成体系插入非天然氨基酸的方法
WO2024035661A1 (en) 2022-08-08 2024-02-15 Sutro Biopharma, Inc. Spray dried extract

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6337191B1 (en) 1999-03-22 2002-01-08 The Board Of Trustees Of The Leland Stanford Junior University Vitro protein synthesis using glycolytic intermediates as an energy source
DK1456360T3 (da) 2001-04-19 2015-08-31 Scripps Research Inst Fremgangsmåder og sammensætning til fremstiling af ortogonale TRNA-aminoacyl-TRNA-syntetasepar
SI1539948T1 (sl) * 2002-08-19 2010-02-26 Univ Leland Stanford Junior Izboljšani postopki sinteze proteinov in vitro
JP3896460B2 (ja) 2003-03-04 2007-03-22 国立大学法人岐阜大学 非天然蛋白質の製造方法、固定化方法及びキット
US20050054044A1 (en) 2003-07-18 2005-03-10 The Board Of Trustees Of The Leland Stanford Junior University Method of alleviating nucleotide limitations for in vitro protein synthesis
US7341852B2 (en) 2003-07-18 2008-03-11 The Board Of Trustees Of The Leland Stanford Junior University Methods of decoupling reaction scale and protein synthesis yield in batch mode
EP1685240B1 (en) 2003-11-20 2014-12-31 The Board of Trustees of the Leland Stanford Junior University Improved methods of in vitro protein synthesis
AU2007248680C1 (en) * 2006-05-02 2014-01-23 Allozyne, Inc. Non-natural amino acid substituted polypeptides

Also Published As

Publication number Publication date
CA2657811A1 (en) 2008-06-05
EP2035554B1 (en) 2013-04-24
WO2008066583A3 (en) 2008-12-18
US20100093024A1 (en) 2010-04-15
AU2007325952A1 (en) 2008-06-05
AU2007325952B2 (en) 2008-06-05
WO2008066583A2 (en) 2008-06-05
ES2415635T3 (es) 2013-07-26
AU2007325952A8 (en) 2013-06-27
US8715958B2 (en) 2014-05-06
CA2657811C (en) 2014-10-21
AU2007325952B8 (en) 2013-06-27
JP5620100B2 (ja) 2014-11-05
EP2035554A2 (en) 2009-03-18
CN101479379B (zh) 2012-01-18
AU2007325952A2 (en) 2009-05-14
JP2009542214A (ja) 2009-12-03
CN101479379A (zh) 2009-07-08
EP2035554A4 (en) 2012-02-22
WO2008066583A9 (en) 2008-07-24

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