SG10202108497TA - Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones - Google Patents
Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperonesInfo
- Publication number
- SG10202108497TA SG10202108497TA SG10202108497TA SG10202108497TA SG10202108497TA SG 10202108497T A SG10202108497T A SG 10202108497TA SG 10202108497T A SG10202108497T A SG 10202108497TA SG 10202108497T A SG10202108497T A SG 10202108497TA SG 10202108497T A SG10202108497T A SG 10202108497TA
- Authority
- SG
- Singapore
- Prior art keywords
- expression
- biologically active
- elevated levels
- synthesis system
- active proteins
- Prior art date
Links
- 102000005431 Molecular Chaperones Human genes 0.000 title 1
- 108010006519 Molecular Chaperones Proteins 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 title 1
- 230000015572 biosynthetic process Effects 0.000 title 1
- 239000000284 extract Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361813914P | 2013-04-19 | 2013-04-19 | |
| US201461937069P | 2014-02-07 | 2014-02-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10202108497TA true SG10202108497TA (en) | 2021-09-29 |
Family
ID=50733442
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201508642WA SG11201508642WA (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
| SG10202108497TA SG10202108497TA (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201508642WA SG11201508642WA (en) | 2013-04-19 | 2014-04-18 | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US10190145B2 (enExample) |
| EP (1) | EP2986731B1 (enExample) |
| JP (2) | JP6463732B2 (enExample) |
| KR (1) | KR102289258B1 (enExample) |
| CN (1) | CN105283556B (enExample) |
| AU (1) | AU2014253785B2 (enExample) |
| CA (1) | CA2909516C (enExample) |
| DK (1) | DK2986731T3 (enExample) |
| ES (1) | ES2922358T3 (enExample) |
| HU (1) | HUE059565T2 (enExample) |
| IL (1) | IL242148B (enExample) |
| SG (2) | SG11201508642WA (enExample) |
| WO (1) | WO2014172631A2 (enExample) |
Families Citing this family (51)
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| KR102289258B1 (ko) * | 2013-04-19 | 2021-08-11 | 서트로 바이오파마, 인크. | 상승된 수준의 외인성 샤페론을 갖는 세포 추출물을 이용한 박테리아 세포 비함유 합성 시스템에서의 생물학적 활성 단백질의 발현 |
| EP3164501A4 (en) | 2014-07-02 | 2018-01-17 | Sutro Biopharma, Inc. | High growth capacity auxotrophic escherichia coli and methods of use |
| RU2739500C2 (ru) | 2014-11-05 | 2020-12-25 | Дженентек, Инк. | Способы получения двуцепочечных белков в бактериях |
| CN108064308B (zh) | 2014-11-05 | 2023-06-09 | 豪夫迈·罗氏有限公司 | 细菌中产生双链蛋白的方法 |
| US10487133B2 (en) | 2014-12-19 | 2019-11-26 | Sutro Biopharma, Inc. | Codon optimization for titer and fidelity improvement |
| MX391051B (es) * | 2014-12-30 | 2025-03-21 | Celgene Corp | Anticuerpos anti-cd47 y usos de los mismos. |
| CN107531749A (zh) * | 2015-03-06 | 2018-01-02 | 豪夫迈·罗氏有限公司 | 超纯化DsbA和DsbC及其制备和使用方法 |
| US20170029798A1 (en) * | 2015-07-27 | 2017-02-02 | Cellivery Therapeutics, Inc. | Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD) |
| WO2017031476A2 (en) * | 2015-08-20 | 2017-02-23 | Genentech, Inc. | Purification of fkpa and uses thereof for producing recombinant polypeptides |
| CN105198983B (zh) * | 2015-09-11 | 2018-11-06 | 中国石油大学(华东) | 一种利于七次跨膜蛋白ccr5功能性表达的方法 |
| CN106190940B (zh) * | 2016-07-19 | 2019-06-14 | 北京三元基因药业股份有限公司 | 表达抗TNF抗体Fab片段的重组大肠杆菌工程菌 |
| CN106399352B (zh) * | 2016-11-09 | 2020-04-17 | 华东理工大学 | 调节目标蛋白表达的折叠因子及其应用 |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| US12227778B2 (en) | 2016-12-30 | 2025-02-18 | Nature's Toolbox, Inc. | Cell-free expression system having novel inorganic polyphosphate-based energy regeneration |
| MX2019007910A (es) | 2016-12-30 | 2019-12-05 | Sutrovax Inc | Conjugados de polipeptido-antigeno con aminoacidos no naturales. |
| DK3562940T3 (da) * | 2016-12-30 | 2022-07-11 | Natures Toolbox Inc | Cellefrit ekspressionssystem med hidtil ukendt, uorganisk polyphosphatbaseret energiregeneration |
| JP7325341B2 (ja) | 2017-07-11 | 2023-08-14 | シンソークス，インク． | 非天然ヌクレオチドの組み込み及びその方法 |
| BR112020002272A2 (pt) | 2017-08-03 | 2020-07-28 | Synthorx, Inc. | conjugados de citocina para o tratamento de doenças autoimunes |
| US20200181670A1 (en) * | 2017-08-11 | 2020-06-11 | Synvitrobio, Inc. | Improved In Vitro Transcription/Translation (TXTL) System and Use Thereof |
| WO2019127469A1 (zh) * | 2017-12-29 | 2019-07-04 | 康码（上海）生物科技有限公司 | 一种高效的能源再生体系(bes)、试剂盒及其制备方法 |
| KR102812714B1 (ko) | 2018-02-26 | 2025-05-27 | 신톡스, 인크. | Il-15 접합체 및 이의 용도 |
| SG11202100023XA (en) | 2018-07-11 | 2021-01-28 | Actym Therapeutics Inc | Engineered immunostimulatory bacterial strains and uses thereof |
| WO2020096959A1 (en) | 2018-11-05 | 2020-05-14 | Genentech, Inc. | Methods of producing two chain proteins in prokaryotic host cells |
| JP7636323B2 (ja) | 2018-11-08 | 2025-02-26 | シンソークス， インコーポレイテッド | インターロイキン１０コンジュゲートおよびその使用 |
| WO2020097385A1 (en) * | 2018-11-08 | 2020-05-14 | Sutro Biopharma, Inc. | E coli strains having an oxidative cytoplasm |
| CN109371049A (zh) * | 2018-11-14 | 2019-02-22 | 天津大学 | 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用 |
| CN113660946A (zh) | 2019-02-06 | 2021-11-16 | 新索思股份有限公司 | Il-2缀合物及其使用方法 |
| US12024709B2 (en) | 2019-02-27 | 2024-07-02 | Actym Therapeutics, Inc. | Immunostimulatory bacteria engineered to colonize tumors, tumor-resident immune cells, and the tumor microenvironment |
| WO2021178001A1 (en) * | 2020-03-05 | 2021-09-10 | Curie Co. Inc. | Methods for cell free protein expression of mature polypeptides derived from zymogens and proproteins |
| MX2022001776A (es) | 2019-08-15 | 2022-03-17 | Synthorx Inc | Terapias de combinacion de inmuno oncologia con conjugados de il-2. |
| JP7758662B2 (ja) | 2019-09-10 | 2025-10-22 | シンソークス， インコーポレイテッド | Ｉｌ－２コンジュゲートおよび自己免疫疾患を治療するための使用方法 |
| KR20220097445A (ko) | 2019-11-04 | 2022-07-07 | 신톡스, 인크. | 인터류킨 10 접합체 및 이의 용도 |
| EP4056584A4 (en) * | 2019-11-08 | 2024-06-05 | Korea Institute of Ocean Science & Technology | NOVEL HUMAN EPIDERMAL GROWTH FACTOR-TF FUSION PROTEIN AND USE THEREOF |
| CA3175683A1 (en) * | 2020-04-29 | 2021-11-04 | Xueyang FENG | Compositions and methods for enhancing recombinant biosynthesis of cannabinoids |
| EP4171648A1 (en) | 2020-06-25 | 2023-05-03 | Synthorx, Inc. | Immuno oncology combination therapy with il-2 conjugates and anti-egfr antibodies |
| KR20220000028U (ko) | 2020-06-29 | 2022-01-05 | 채인자 | 농작물용 농약 간편 분무장치 |
| AU2021342284A1 (en) * | 2020-09-14 | 2023-04-20 | Sutro Biopharma, Inc. | Method for large scale production of antibodies using a cell-free protein synthesis system |
| MX2023004029A (es) | 2020-10-09 | 2023-04-27 | Synthorx Inc | Terapia de combinacion de inmunoncologia con conjugados de il-2 y pembrolizumab. |
| EP4225375A1 (en) | 2020-10-09 | 2023-08-16 | Synthorx, Inc. | Immuno oncology therapies with il-2 conjugates |
| EP4291243A1 (en) | 2021-02-12 | 2023-12-20 | Synthorx, Inc. | Lung cancer combination therapy with il-2 conjugates and an anti-pd-1 antibody or antigen-binding fragment thereof |
| TW202245843A (zh) | 2021-02-12 | 2022-12-01 | 美商欣爍克斯公司 | Ｉｌ－２接合物及西米普利單抗（ｃｅｍｉｐｌｉｍａｂ）之皮膚癌組合療法 |
| TW202313117A (zh) | 2021-06-03 | 2023-04-01 | 美商欣爍克斯公司 | 包含il-2接合物及西妥昔單抗(cetuximab)之頭頸癌組合療法 |
| EP4452327A1 (en) | 2021-12-20 | 2024-10-30 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122750A1 (en) | 2021-12-23 | 2023-06-29 | Synthorx, Inc. | Cancer combination therapy with il-2 conjugates and cetuximab |
| US12163190B2 (en) * | 2022-06-17 | 2024-12-10 | Insitro, Inc. | In situ sequencing of RNA transcripts with non-uniform 5 prime ends |
| CN115028691B (zh) * | 2022-06-29 | 2024-09-10 | 青岛硕景生物科技有限公司 | 一种检测试纸用的独立质控系统、杂交瘤细胞株、质控线用包被单克隆抗体及其应用 |
| WO2024136899A1 (en) | 2022-12-21 | 2024-06-27 | Synthorx, Inc. | Cancer therapy with il-2 conjugates and chimeric antigen receptor therapies |
| WO2024196937A1 (en) | 2023-03-20 | 2024-09-26 | Synthorx, Inc. | Cancer therapy with il-2 peg conjugates |
| CN116925196B (zh) * | 2023-06-08 | 2025-04-18 | 齐鲁工业大学(山东省科学院) | 小分子热休克蛋白rsp_1572在提高宿主菌环境耐受性中的应用 |
| WO2025158385A1 (en) | 2024-01-25 | 2025-07-31 | Genzyme Corporation | Pegylated il-2 for suppressing adaptive immune response to gene therapy |
| CN118048325B (zh) * | 2024-04-16 | 2024-07-16 | 天津凯莱英生物科技有限公司 | 酶的高通量筛选方法及酶库的构建方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10145694A1 (de) | 2001-09-17 | 2003-04-03 | Roche Diagnostics Gmbh | Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung |
| US20060134739A1 (en) | 2003-03-11 | 2006-06-22 | Chatterjee Deb K | Synthesis of proteins by cell-free protein expression |
| JP2005253432A (ja) | 2004-03-15 | 2005-09-22 | Sekisui Chem Co Ltd | タンパク質の生産方法、並びにそれに使用するための組成物、試薬及びキット |
| JP4561243B2 (ja) | 2004-08-27 | 2010-10-13 | 株式会社豊田中央研究所 | 無細胞タンパク質合成系のための媒体 |
| AU2007325952B8 (en) * | 2006-06-29 | 2013-06-27 | The Board Of Trustees Of The Leland Stanford Junior University | Cell-free synthesis of proteins containing unnatural amino acids |
| US7871794B2 (en) * | 2007-01-18 | 2011-01-18 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced cell-free synthesis of active proteins containing disulfide bonds |
| EP2376647B1 (en) | 2009-01-12 | 2014-03-05 | Sutro Biopharma, Inc. | Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system |
| CN102732548A (zh) | 2012-05-16 | 2012-10-17 | 中国农业大学 | 一种表达蛇毒激肽原酶的高效麦胚无细胞蛋白合成系统的建立及应用 |
| CA2887355C (en) * | 2012-10-12 | 2023-03-07 | Sutro Biopharma, Inc. | Proteolytic inactivation of select proteins in bacterial extracts for improved expression |
| KR102289258B1 (ko) * | 2013-04-19 | 2021-08-11 | 서트로 바이오파마, 인크. | 상승된 수준의 외인성 샤페론을 갖는 세포 추출물을 이용한 박테리아 세포 비함유 합성 시스템에서의 생물학적 활성 단백질의 발현 |
-
2014
- 2014-04-18 KR KR1020157032515A patent/KR102289258B1/ko active Active
- 2014-04-18 WO PCT/US2014/034643 patent/WO2014172631A2/en not_active Ceased
- 2014-04-18 CN CN201480032258.8A patent/CN105283556B/zh active Active
- 2014-04-18 US US14/256,324 patent/US10190145B2/en active Active
- 2014-04-18 DK DK14724974.2T patent/DK2986731T3/da active
- 2014-04-18 CA CA2909516A patent/CA2909516C/en active Active
- 2014-04-18 ES ES14724974T patent/ES2922358T3/es active Active
- 2014-04-18 AU AU2014253785A patent/AU2014253785B2/en active Active
- 2014-04-18 EP EP14724974.2A patent/EP2986731B1/en active Active
- 2014-04-18 SG SG11201508642WA patent/SG11201508642WA/en unknown
- 2014-04-18 JP JP2016509127A patent/JP6463732B2/ja active Active
- 2014-04-18 HU HUE14724974A patent/HUE059565T2/hu unknown
- 2014-04-18 SG SG10202108497TA patent/SG10202108497TA/en unknown
-
2015
- 2015-10-18 IL IL242148A patent/IL242148B/en active IP Right Grant
-
2018
- 2018-07-20 JP JP2018136909A patent/JP2018157837A/ja not_active Withdrawn
- 2018-12-03 US US16/208,245 patent/US10774354B2/en active Active
-
2020
- 2020-08-12 US US16/991,607 patent/US20210054429A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2986731B1 (en) | 2022-05-18 |
| SG11201508642WA (en) | 2015-11-27 |
| EP2986731A2 (en) | 2016-02-24 |
| DK2986731T3 (da) | 2022-08-01 |
| AU2014253785B2 (en) | 2018-07-19 |
| US20140315245A1 (en) | 2014-10-23 |
| JP2016518833A (ja) | 2016-06-30 |
| HK1220993A1 (en) | 2017-05-19 |
| CA2909516A1 (en) | 2014-10-23 |
| US20210054429A1 (en) | 2021-02-25 |
| KR102289258B1 (ko) | 2021-08-11 |
| WO2014172631A3 (en) | 2014-12-18 |
| JP2018157837A (ja) | 2018-10-11 |
| AU2014253785A1 (en) | 2015-11-05 |
| US10774354B2 (en) | 2020-09-15 |
| JP6463732B2 (ja) | 2019-02-06 |
| HUE059565T2 (hu) | 2022-11-28 |
| WO2014172631A4 (en) | 2015-02-19 |
| CN105283556B (zh) | 2020-07-14 |
| CA2909516C (en) | 2023-03-07 |
| KR20160002913A (ko) | 2016-01-08 |
| ES2922358T3 (es) | 2022-09-13 |
| IL242148B (en) | 2019-03-31 |
| CN105283556A (zh) | 2016-01-27 |
| US10190145B2 (en) | 2019-01-29 |
| US20190161781A1 (en) | 2019-05-30 |
| WO2014172631A2 (en) | 2014-10-23 |
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