JP2015516165A5 - - Google Patents

Info

Publication number

JP2015516165A5

JP2015516165A5 JP2015511949A JP2015511949A JP2015516165A5 JP 2015516165 A5 JP2015516165 A5 JP 2015516165A5 JP 2015511949 A JP2015511949 A JP 2015511949A JP 2015511949 A JP2015511949 A JP 2015511949A JP 2015516165 A5 JP2015516165 A5 JP 2015516165A5

Authority

JP

Japan

Prior art keywords

polymerase

amino acid

acid sequence

seq

activity

Prior art date

2013-05-16

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 125000003275 alpha amino acid group Chemical group 0.000 claims 69
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 25
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 25
• 230000000694 effects Effects 0.000 claims 24
• 238000000034 method Methods 0.000 claims 24
• 102000004169 proteins and genes Human genes 0.000 claims 21
• 108090000623 proteins and genes Proteins 0.000 claims 21
• 102000004190 Enzymes Human genes 0.000 claims 13
• 108090000790 Enzymes Proteins 0.000 claims 13
• 150000001413 amino acids Chemical class 0.000 claims 12
• -1 Pyrobest polymerase Proteins 0.000 claims 11
• 150000008574 D-amino acids Chemical class 0.000 claims 10
• 239000002773 nucleotide Substances 0.000 claims 9
• 239000012634 fragment Substances 0.000 claims 7
• 241000701386 African swine fever virus Species 0.000 claims 6
• 108010006785 Taq Polymerase Proteins 0.000 claims 6
• BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims 6
• 102000035195 Peptidases Human genes 0.000 claims 4
• 108091005804 Peptidases Proteins 0.000 claims 4
• 241000589499 Thermus thermophilus Species 0.000 claims 4
• 235000019833 protease Nutrition 0.000 claims 4
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 3
• 108010017826 DNA Polymerase I Proteins 0.000 claims 3
• 102000004594 DNA Polymerase I Human genes 0.000 claims 3
• 108050009160 DNA polymerase 1 Proteins 0.000 claims 3
• 108010019653 Pwo polymerase Proteins 0.000 claims 3
• 241001467519 Pyrococcus sp. Species 0.000 claims 3
• 101100117473 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) dbh gene Proteins 0.000 claims 3
• 241000205188 Thermococcus Species 0.000 claims 3
• 108010020713 Tth polymerase Proteins 0.000 claims 3
• 108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 claims 3
• 230000002255 enzymatic effect Effects 0.000 claims 3
• 229910052697 platinum Inorganic materials 0.000 claims 3
• 241000205156 Pyrococcus furiosus Species 0.000 claims 2
• 101900232935 Pyrococcus furiosus DNA polymerase Proteins 0.000 claims 2
• 241000205091 Sulfolobus solfataricus Species 0.000 claims 2
• 241000205180 Thermococcus litoralis Species 0.000 claims 2
• 108010001244 Tli polymerase Proteins 0.000 claims 2
• 125000004185 ester group Chemical group 0.000 claims 2
• 108020004707 nucleic acids Proteins 0.000 claims 2
• 102000039446 nucleic acids Human genes 0.000 claims 2
• 241000205160 Pyrococcus Species 0.000 claims 1
• 241000205101 Sulfolobus Species 0.000 claims 1
• 230000003321 amplification Effects 0.000 claims 1
• 238000006243 chemical reaction Methods 0.000 claims 1
• 238000009833 condensation Methods 0.000 claims 1
• 230000005494 condensation Effects 0.000 claims 1
• 238000004519 manufacturing process Methods 0.000 claims 1
• 238000012986 modification Methods 0.000 claims 1
• 230000004048 modification Effects 0.000 claims 1
• 238000003199 nucleic acid amplification method Methods 0.000 claims 1
• 150000007523 nucleic acids Chemical class 0.000 claims 1
• 125000003729 nucleotide group Chemical group 0.000 claims 1
• 238000010647 peptide synthesis reaction Methods 0.000 claims 1
• 238000003752 polymerase chain reaction Methods 0.000 claims 1
• 125000006239 protecting group Chemical group 0.000 claims 1
• 239000007790 solid phase Substances 0.000 claims 1
• 239000000126 substance Substances 0.000 claims 1
• 125000005000 thioaryl group Chemical group 0.000 claims 1