CN104379748B - L-核酸的酶促合成 - Google Patents

L-核酸的酶促合成 Download PDF

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Publication number
CN104379748B
CN104379748B CN201380024198.0A CN201380024198A CN104379748B CN 104379748 B CN104379748 B CN 104379748B CN 201380024198 A CN201380024198 A CN 201380024198A CN 104379748 B CN104379748 B CN 104379748B
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amino acid
acid sequence
polymerase
seq
nucleic acid
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Chinese (zh)
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CN104379748A (zh
Inventor
A·佩什
R·大卫
F·雅罗什
M·雅恩兹
S·克鲁斯曼
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Arterian Biotechnology Inc
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Noxxon Pharma AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201380024198.0A 2012-05-16 2013-05-16 L-核酸的酶促合成 Active CN104379748B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811516622.4A CN109554418A (zh) 2012-05-16 2013-05-16 L-核酸的酶促合成

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12003887 2012-05-16
EP12003887.2 2012-05-16
PCT/EP2013/001458 WO2013170963A2 (en) 2012-05-16 2013-05-16 Enzymatic synthesis of l-nucleic acids

Related Child Applications (1)

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CN201811516622.4A Division CN109554418A (zh) 2012-05-16 2013-05-16 L-核酸的酶促合成

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CN104379748A CN104379748A (zh) 2015-02-25
CN104379748B true CN104379748B (zh) 2019-01-04

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CN201811516622.4A Pending CN109554418A (zh) 2012-05-16 2013-05-16 L-核酸的酶促合成

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US (2) US9850471B2 (enExample)
EP (1) EP2850192B1 (enExample)
JP (1) JP6522496B2 (enExample)
KR (1) KR102168915B1 (enExample)
CN (2) CN104379748B (enExample)
AU (1) AU2013262069C1 (enExample)
CA (1) CA2873452A1 (enExample)
ES (1) ES2965777T3 (enExample)
IL (1) IL235556B (enExample)
RU (1) RU2704833C2 (enExample)
WO (1) WO2013170963A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2873452A1 (en) 2012-05-16 2013-11-21 Noxxon Pharma Ag Enzymatic synthesis of l-nucleic acids
SG11201506885UA (en) 2013-03-21 2015-09-29 Sanofi Aventis Deutschland Synthesis of cyclic imide containing peptide products
HK1219477A1 (zh) 2013-03-21 2017-04-07 Sanofi-Aventis Deutschland Gmbh 合成含有乙内酰脲的肽产物
JP6670237B2 (ja) * 2013-11-20 2020-03-25 アプタリオン バイオテック エージー 酵素によるｌ−核酸の合成
CN106467910A (zh) * 2015-08-18 2017-03-01 清华大学 L-dna/l-rna聚合酶及其应用
ES2846949T3 (es) 2015-11-16 2021-07-30 Stratos Genomics Inc Variantes de la polimerasa DPO4
WO2018204707A1 (en) * 2017-05-04 2018-11-08 Stratos Genomics Inc. Dp04 polymerase variants
AU2018383610B2 (en) * 2017-12-11 2024-09-12 F. Hoffmann-La Roche Ag DPO4 polymerase variants with improved accuracy
KR102285583B1 (ko) * 2020-01-09 2021-08-05 한국과학기술연구원 L-dna와 결합하는 자연계 단백질 및 이의 용도
EP4473115A1 (en) * 2022-02-03 2024-12-11 Tsinghua University Mirror-image selection of l-nucleic acid aptamers

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2003047743A2 (de) * 2001-11-16 2003-06-12 Noxxon Pharma Ag Verfahren zur synthese von all-d-peptide und peptidnukleinsäuren

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US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
JPH08505872A (ja) 1993-01-22 1996-06-25 ユニバーシティ・リサーチ・コーポレイション 治療剤の局所化
JP2001504448A (ja) * 1996-08-30 2001-04-03 フュルステ，イェンス，ペーター 核酸の鏡面対称選択および進化
EP1288309A1 (de) 2001-08-30 2003-03-05 Noxxon Pharma AG Verfahren zur Markierung von L-Nukleinsäuren
ES2346640T4 (es) * 2001-10-26 2011-04-26 Noxxon Pharma Ag Ácido l-nucleico modificado.
DE10156274A1 (de) 2001-11-16 2003-06-12 Noxxon Pharma Ag Verfahren zur Synthese von all-D-Polypeptiden
KR20060015505A (ko) 2003-04-13 2006-02-17 엔존 파마슈티컬즈, 인코포레이티드 올리고뉴클레오타이드 전구약물
US20090281296A1 (en) 2004-02-09 2009-11-12 Supramol Parenteral Colloid Gmbh Process for the production of conjugates from polysaccharides and polynucelotides
WO2006028496A2 (en) * 2004-02-20 2006-03-16 Applera Corporation Lesion repair polymerase compositions
ES2741524T3 (es) 2004-03-23 2020-02-11 Ascendis Pharma Gmbh Profármaco polimérico con un enlazador autoinmolador
WO2006052790A2 (en) 2004-11-05 2006-05-18 The Children's Hospital Of Philadelphia Biodegradable linkers for molecular therapies
CN101534643A (zh) 2006-09-15 2009-09-16 安佐制药股份有限公司 用于递送寡核苷酸的基于位阻酯的生物可降解连接体
JP5637857B2 (ja) * 2008-10-28 2014-12-10 学校法人関西学院 リフォールディング剤および蛋白質のリフォールディング方法
CA2873452A1 (en) 2012-05-16 2013-11-21 Noxxon Pharma Ag Enzymatic synthesis of l-nucleic acids

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Publication number Publication date
JP6522496B2 (ja) 2019-05-29
EP2850192B1 (en) 2023-11-01
KR102168915B1 (ko) 2020-10-23
AU2013262069C1 (en) 2019-01-31
EP2850192A2 (en) 2015-03-25
IL235556B (en) 2020-02-27
RU2704833C2 (ru) 2019-10-31
EP2850192C0 (en) 2023-11-01
CN104379748A (zh) 2015-02-25
ES2965777T3 (es) 2024-04-16
US11015178B2 (en) 2021-05-25
CN109554418A (zh) 2019-04-02
IL235556A0 (en) 2015-01-29
JP2015516165A (ja) 2015-06-11
US20180208910A1 (en) 2018-07-26
US9850471B2 (en) 2017-12-26
AU2013262069B2 (en) 2018-04-05
CA2873452A1 (en) 2013-11-21
WO2013170963A3 (en) 2014-01-23
RU2014140243A (ru) 2016-07-10
KR20150013804A (ko) 2015-02-05
WO2013170963A2 (en) 2013-11-21
AU2013262069A1 (en) 2014-10-23
US20150337348A1 (en) 2015-11-26

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Patentee after: Arterian biotechnology, Inc

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Patentee before: Nozon Pharmaceutical Co., Ltd.