AU2013262069C1 - Enzymatic synthesis of L-nucleic acids - Google Patents

Enzymatic synthesis of L-nucleic acids Download PDF

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Publication number
AU2013262069C1
AU2013262069C1 AU2013262069A AU2013262069A AU2013262069C1 AU 2013262069 C1 AU2013262069 C1 AU 2013262069C1 AU 2013262069 A AU2013262069 A AU 2013262069A AU 2013262069 A AU2013262069 A AU 2013262069A AU 2013262069 C1 AU2013262069 C1 AU 2013262069C1
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Prior art keywords
polymerase
nucleic acid
amino acid
acid sequence
dna
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AU2013262069A
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AU2013262069B2 (en
AU2013262069A1 (en
Inventor
Ralf DAVID
Michael Jahnz
Florian Jarosch
Sven Klussmann
Andreas Pech
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Aptarion Biotech AG
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Aptarion Biotech AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2013262069A 2012-05-16 2013-05-16 Enzymatic synthesis of L-nucleic acids Active AU2013262069C1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12003887 2012-05-16
EP12003887.2 2012-05-16
PCT/EP2013/001458 WO2013170963A2 (en) 2012-05-16 2013-05-16 Enzymatic synthesis of l-nucleic acids

Publications (3)

Publication Number Publication Date
AU2013262069A1 AU2013262069A1 (en) 2014-10-23
AU2013262069B2 AU2013262069B2 (en) 2018-04-05
AU2013262069C1 true AU2013262069C1 (en) 2019-01-31

Family

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AU2013262069A Active AU2013262069C1 (en) 2012-05-16 2013-05-16 Enzymatic synthesis of L-nucleic acids

Country Status (11)

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US (2) US9850471B2 (enExample)
EP (1) EP2850192B1 (enExample)
JP (1) JP6522496B2 (enExample)
KR (1) KR102168915B1 (enExample)
CN (2) CN104379748B (enExample)
AU (1) AU2013262069C1 (enExample)
CA (1) CA2873452A1 (enExample)
ES (1) ES2965777T3 (enExample)
IL (1) IL235556B (enExample)
RU (1) RU2704833C2 (enExample)
WO (1) WO2013170963A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2873452A1 (en) 2012-05-16 2013-11-21 Noxxon Pharma Ag Enzymatic synthesis of l-nucleic acids
SG11201506885UA (en) 2013-03-21 2015-09-29 Sanofi Aventis Deutschland Synthesis of cyclic imide containing peptide products
HK1219477A1 (zh) 2013-03-21 2017-04-07 Sanofi-Aventis Deutschland Gmbh 合成含有乙内酰脲的肽产物
JP6670237B2 (ja) * 2013-11-20 2020-03-25 アプタリオン バイオテック エージー 酵素によるｌ−核酸の合成
CN106467910A (zh) * 2015-08-18 2017-03-01 清华大学 L-dna/l-rna聚合酶及其应用
ES2846949T3 (es) 2015-11-16 2021-07-30 Stratos Genomics Inc Variantes de la polimerasa DPO4
WO2018204707A1 (en) * 2017-05-04 2018-11-08 Stratos Genomics Inc. Dp04 polymerase variants
AU2018383610B2 (en) * 2017-12-11 2024-09-12 F. Hoffmann-La Roche Ag DPO4 polymerase variants with improved accuracy
KR102285583B1 (ko) * 2020-01-09 2021-08-05 한국과학기술연구원 L-dna와 결합하는 자연계 단백질 및 이의 용도
EP4473115A1 (en) * 2022-02-03 2024-12-11 Tsinghua University Mirror-image selection of l-nucleic acid aptamers

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008856A2 (de) * 1996-08-30 1998-03-05 Fuerste Jens Peter Spiegelselektion und spiegelevolution von nucleinsäuren

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US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
JPH08505872A (ja) 1993-01-22 1996-06-25 ユニバーシティ・リサーチ・コーポレイション 治療剤の局所化
EP1288309A1 (de) 2001-08-30 2003-03-05 Noxxon Pharma AG Verfahren zur Markierung von L-Nukleinsäuren
ES2346640T4 (es) * 2001-10-26 2011-04-26 Noxxon Pharma Ag Ácido l-nucleico modificado.
EP1312680A1 (de) 2001-11-16 2003-05-21 Noxxon Pharma AG Verfahren zur Synthese von all-D-Peptiden und Peptidnukleinsäuren
DE10156274A1 (de) 2001-11-16 2003-06-12 Noxxon Pharma Ag Verfahren zur Synthese von all-D-Polypeptiden
KR20060015505A (ko) 2003-04-13 2006-02-17 엔존 파마슈티컬즈, 인코포레이티드 올리고뉴클레오타이드 전구약물
US20090281296A1 (en) 2004-02-09 2009-11-12 Supramol Parenteral Colloid Gmbh Process for the production of conjugates from polysaccharides and polynucelotides
WO2006028496A2 (en) * 2004-02-20 2006-03-16 Applera Corporation Lesion repair polymerase compositions
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WO2006052790A2 (en) 2004-11-05 2006-05-18 The Children's Hospital Of Philadelphia Biodegradable linkers for molecular therapies
CN101534643A (zh) 2006-09-15 2009-09-16 安佐制药股份有限公司 用于递送寡核苷酸的基于位阻酯的生物可降解连接体
JP5637857B2 (ja) * 2008-10-28 2014-12-10 学校法人関西学院 リフォールディング剤および蛋白質のリフォールディング方法
CA2873452A1 (en) 2012-05-16 2013-11-21 Noxxon Pharma Ag Enzymatic synthesis of l-nucleic acids

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008856A2 (de) * 1996-08-30 1998-03-05 Fuerste Jens Peter Spiegelselektion und spiegelevolution von nucleinsäuren

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BOUDSOCQ F ET AL., "Sulffolobus solfataricus P2 DNA polymerase IV(Dpo4):an archeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic pol", NUCLEIC ACIDS RESEARCH. 2001, vol. 29, no. 22, pages 4607 - 4616. *
OLIVEROS M ET AL., "Characterization of an African swine fever virus 20-kDa DNA polymerase involved in DNA repair", THE JOURNAL OF BIOLOGICAL CHEMISTRY. 1997, vol. 272, no. 49, pages 30899 - 30910. *

Also Published As

Publication number Publication date
JP6522496B2 (ja) 2019-05-29
EP2850192B1 (en) 2023-11-01
KR102168915B1 (ko) 2020-10-23
EP2850192A2 (en) 2015-03-25
IL235556B (en) 2020-02-27
RU2704833C2 (ru) 2019-10-31
EP2850192C0 (en) 2023-11-01
CN104379748A (zh) 2015-02-25
ES2965777T3 (es) 2024-04-16
US11015178B2 (en) 2021-05-25
CN109554418A (zh) 2019-04-02
CN104379748B (zh) 2019-01-04
IL235556A0 (en) 2015-01-29
JP2015516165A (ja) 2015-06-11
US20180208910A1 (en) 2018-07-26
US9850471B2 (en) 2017-12-26
AU2013262069B2 (en) 2018-04-05
CA2873452A1 (en) 2013-11-21
WO2013170963A3 (en) 2014-01-23
RU2014140243A (ru) 2016-07-10
KR20150013804A (ko) 2015-02-05
WO2013170963A2 (en) 2013-11-21
AU2013262069A1 (en) 2014-10-23
US20150337348A1 (en) 2015-11-26

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