CA2873452A1 - Enzymatic synthesis of l-nucleic acids - Google Patents
Enzymatic synthesis of l-nucleic acids Download PDFInfo
- Publication number
- CA2873452A1 CA2873452A1 CA2873452A CA2873452A CA2873452A1 CA 2873452 A1 CA2873452 A1 CA 2873452A1 CA 2873452 A CA2873452 A CA 2873452A CA 2873452 A CA2873452 A CA 2873452A CA 2873452 A1 CA2873452 A1 CA 2873452A1
- Authority
- CA
- Canada
- Prior art keywords
- polymerase
- amino acid
- acid sequence
- seq
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12003887 | 2012-05-16 | ||
| EP12003887.2 | 2012-05-16 | ||
| PCT/EP2013/001458 WO2013170963A2 (en) | 2012-05-16 | 2013-05-16 | Enzymatic synthesis of l-nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2873452A1 true CA2873452A1 (en) | 2013-11-21 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2873452A Abandoned CA2873452A1 (en) | 2012-05-16 | 2013-05-16 | Enzymatic synthesis of l-nucleic acids |
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| Country | Link |
|---|---|
| US (2) | US9850471B2 (enExample) |
| EP (1) | EP2850192B1 (enExample) |
| JP (1) | JP6522496B2 (enExample) |
| KR (1) | KR102168915B1 (enExample) |
| CN (2) | CN104379748B (enExample) |
| AU (1) | AU2013262069C1 (enExample) |
| CA (1) | CA2873452A1 (enExample) |
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| RU (1) | RU2704833C2 (enExample) |
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| CA2873452A1 (en) | 2012-05-16 | 2013-11-21 | Noxxon Pharma Ag | Enzymatic synthesis of l-nucleic acids |
| SG11201506885UA (en) | 2013-03-21 | 2015-09-29 | Sanofi Aventis Deutschland | Synthesis of cyclic imide containing peptide products |
| HK1219477A1 (zh) | 2013-03-21 | 2017-04-07 | Sanofi-Aventis Deutschland Gmbh | 合成含有乙内酰脲的肽产物 |
| JP6670237B2 (ja) * | 2013-11-20 | 2020-03-25 | アプタリオン バイオテック エージー | 酵素によるｌ−核酸の合成 |
| CN106467910A (zh) * | 2015-08-18 | 2017-03-01 | 清华大学 | L-dna/l-rna聚合酶及其应用 |
| ES2846949T3 (es) | 2015-11-16 | 2021-07-30 | Stratos Genomics Inc | Variantes de la polimerasa DPO4 |
| WO2018204707A1 (en) * | 2017-05-04 | 2018-11-08 | Stratos Genomics Inc. | Dp04 polymerase variants |
| AU2018383610B2 (en) * | 2017-12-11 | 2024-09-12 | F. Hoffmann-La Roche Ag | DPO4 polymerase variants with improved accuracy |
| KR102285583B1 (ko) * | 2020-01-09 | 2021-08-05 | 한국과학기술연구원 | L-dna와 결합하는 자연계 단백질 및 이의 용도 |
| EP4473115A1 (en) * | 2022-02-03 | 2024-12-11 | Tsinghua University | Mirror-image selection of l-nucleic acid aptamers |
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| US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
| JPH08505872A (ja) | 1993-01-22 | 1996-06-25 | ユニバーシティ・リサーチ・コーポレイション | 治療剤の局所化 |
| JP2001504448A (ja) * | 1996-08-30 | 2001-04-03 | フュルステ，イェンス，ペーター | 核酸の鏡面対称選択および進化 |
| EP1288309A1 (de) | 2001-08-30 | 2003-03-05 | Noxxon Pharma AG | Verfahren zur Markierung von L-Nukleinsäuren |
| ES2346640T4 (es) * | 2001-10-26 | 2011-04-26 | Noxxon Pharma Ag | Ácido l-nucleico modificado. |
| EP1312680A1 (de) | 2001-11-16 | 2003-05-21 | Noxxon Pharma AG | Verfahren zur Synthese von all-D-Peptiden und Peptidnukleinsäuren |
| DE10156274A1 (de) | 2001-11-16 | 2003-06-12 | Noxxon Pharma Ag | Verfahren zur Synthese von all-D-Polypeptiden |
| KR20060015505A (ko) | 2003-04-13 | 2006-02-17 | 엔존 파마슈티컬즈, 인코포레이티드 | 올리고뉴클레오타이드 전구약물 |
| US20090281296A1 (en) | 2004-02-09 | 2009-11-12 | Supramol Parenteral Colloid Gmbh | Process for the production of conjugates from polysaccharides and polynucelotides |
| WO2006028496A2 (en) * | 2004-02-20 | 2006-03-16 | Applera Corporation | Lesion repair polymerase compositions |
| ES2741524T3 (es) | 2004-03-23 | 2020-02-11 | Ascendis Pharma Gmbh | Profármaco polimérico con un enlazador autoinmolador |
| WO2006052790A2 (en) | 2004-11-05 | 2006-05-18 | The Children's Hospital Of Philadelphia | Biodegradable linkers for molecular therapies |
| CN101534643A (zh) | 2006-09-15 | 2009-09-16 | 安佐制药股份有限公司 | 用于递送寡核苷酸的基于位阻酯的生物可降解连接体 |
| JP5637857B2 (ja) * | 2008-10-28 | 2014-12-10 | 学校法人関西学院 | リフォールディング剤および蛋白質のリフォールディング方法 |
| CA2873452A1 (en) | 2012-05-16 | 2013-11-21 | Noxxon Pharma Ag | Enzymatic synthesis of l-nucleic acids |
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2013
- 2013-05-16 CA CA2873452A patent/CA2873452A1/en not_active Abandoned
- 2013-05-16 RU RU2014140243A patent/RU2704833C2/ru active
- 2013-05-16 EP EP13724519.7A patent/EP2850192B1/en active Active
- 2013-05-16 CN CN201380024198.0A patent/CN104379748B/zh active Active
- 2013-05-16 AU AU2013262069A patent/AU2013262069C1/en active Active
- 2013-05-16 KR KR1020147035228A patent/KR102168915B1/ko active Active
- 2013-05-16 JP JP2015511949A patent/JP6522496B2/ja active Active
- 2013-05-16 WO PCT/EP2013/001458 patent/WO2013170963A2/en not_active Ceased
- 2013-05-16 CN CN201811516622.4A patent/CN109554418A/zh active Pending
- 2013-05-16 ES ES13724519T patent/ES2965777T3/es active Active
- 2013-05-16 US US14/401,128 patent/US9850471B2/en active Active
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2014
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2017
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Also Published As
| Publication number | Publication date |
|---|---|
| JP6522496B2 (ja) | 2019-05-29 |
| EP2850192B1 (en) | 2023-11-01 |
| KR102168915B1 (ko) | 2020-10-23 |
| AU2013262069C1 (en) | 2019-01-31 |
| EP2850192A2 (en) | 2015-03-25 |
| IL235556B (en) | 2020-02-27 |
| RU2704833C2 (ru) | 2019-10-31 |
| EP2850192C0 (en) | 2023-11-01 |
| CN104379748A (zh) | 2015-02-25 |
| ES2965777T3 (es) | 2024-04-16 |
| US11015178B2 (en) | 2021-05-25 |
| CN109554418A (zh) | 2019-04-02 |
| CN104379748B (zh) | 2019-01-04 |
| IL235556A0 (en) | 2015-01-29 |
| JP2015516165A (ja) | 2015-06-11 |
| US20180208910A1 (en) | 2018-07-26 |
| US9850471B2 (en) | 2017-12-26 |
| AU2013262069B2 (en) | 2018-04-05 |
| WO2013170963A3 (en) | 2014-01-23 |
| RU2014140243A (ru) | 2016-07-10 |
| KR20150013804A (ko) | 2015-02-05 |
| WO2013170963A2 (en) | 2013-11-21 |
| AU2013262069A1 (en) | 2014-10-23 |
| US20150337348A1 (en) | 2015-11-26 |
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