RU2704833C2 - Ферментативный синтез l-нуклеиновых кислот - Google Patents
Ферментативный синтез l-нуклеиновых кислот Download PDFInfo
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- RU2704833C2 RU2704833C2 RU2014140243A RU2014140243A RU2704833C2 RU 2704833 C2 RU2704833 C2 RU 2704833C2 RU 2014140243 A RU2014140243 A RU 2014140243A RU 2014140243 A RU2014140243 A RU 2014140243A RU 2704833 C2 RU2704833 C2 RU 2704833C2
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- polymerase
- amino acid
- nucleic acid
- acid sequence
- dna
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- C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
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- C—CHEMISTRY; METALLURGY
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- C—CHEMISTRY; METALLURGY
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
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- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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| EP12003887 | 2012-05-16 | ||
| EP12003887.2 | 2012-05-16 | ||
| PCT/EP2013/001458 WO2013170963A2 (en) | 2012-05-16 | 2013-05-16 | Enzymatic synthesis of l-nucleic acids |
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| RU2014140243A RU2014140243A (ru) | 2016-07-10 |
| RU2704833C2 true RU2704833C2 (ru) | 2019-10-31 |
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| CA2873452A1 (en) | 2012-05-16 | 2013-11-21 | Noxxon Pharma Ag | Enzymatic synthesis of l-nucleic acids |
| SG11201506885UA (en) | 2013-03-21 | 2015-09-29 | Sanofi Aventis Deutschland | Synthesis of cyclic imide containing peptide products |
| HK1219477A1 (zh) | 2013-03-21 | 2017-04-07 | Sanofi-Aventis Deutschland Gmbh | 合成含有乙内酰脲的肽产物 |
| JP6670237B2 (ja) * | 2013-11-20 | 2020-03-25 | アプタリオン バイオテック エージー | 酵素によるｌ−核酸の合成 |
| CN106467910A (zh) * | 2015-08-18 | 2017-03-01 | 清华大学 | L-dna/l-rna聚合酶及其应用 |
| ES2846949T3 (es) | 2015-11-16 | 2021-07-30 | Stratos Genomics Inc | Variantes de la polimerasa DPO4 |
| WO2018204707A1 (en) * | 2017-05-04 | 2018-11-08 | Stratos Genomics Inc. | Dp04 polymerase variants |
| AU2018383610B2 (en) * | 2017-12-11 | 2024-09-12 | F. Hoffmann-La Roche Ag | DPO4 polymerase variants with improved accuracy |
| KR102285583B1 (ko) * | 2020-01-09 | 2021-08-05 | 한국과학기술연구원 | L-dna와 결합하는 자연계 단백질 및 이의 용도 |
| EP4473115A1 (en) * | 2022-02-03 | 2024-12-11 | Tsinghua University | Mirror-image selection of l-nucleic acid aptamers |
Citations (3)
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| WO1998008856A2 (de) * | 1996-08-30 | 1998-03-05 | Fuerste Jens Peter | Spiegelselektion und spiegelevolution von nucleinsäuren |
| WO2003020969A2 (de) * | 2001-08-30 | 2003-03-13 | Noxxon Pharma Ag | Verfahren zur markierung von l-nukleinsäuren |
| WO2003035665A1 (de) * | 2001-10-26 | 2003-05-01 | Noxxon Pharma Ag | Modifizierte l-nukleinsäure |
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| US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
| JPH08505872A (ja) | 1993-01-22 | 1996-06-25 | ユニバーシティ・リサーチ・コーポレイション | 治療剤の局所化 |
| EP1312680A1 (de) | 2001-11-16 | 2003-05-21 | Noxxon Pharma AG | Verfahren zur Synthese von all-D-Peptiden und Peptidnukleinsäuren |
| DE10156274A1 (de) | 2001-11-16 | 2003-06-12 | Noxxon Pharma Ag | Verfahren zur Synthese von all-D-Polypeptiden |
| KR20060015505A (ko) | 2003-04-13 | 2006-02-17 | 엔존 파마슈티컬즈, 인코포레이티드 | 올리고뉴클레오타이드 전구약물 |
| US20090281296A1 (en) | 2004-02-09 | 2009-11-12 | Supramol Parenteral Colloid Gmbh | Process for the production of conjugates from polysaccharides and polynucelotides |
| WO2006028496A2 (en) * | 2004-02-20 | 2006-03-16 | Applera Corporation | Lesion repair polymerase compositions |
| ES2741524T3 (es) | 2004-03-23 | 2020-02-11 | Ascendis Pharma Gmbh | Profármaco polimérico con un enlazador autoinmolador |
| WO2006052790A2 (en) | 2004-11-05 | 2006-05-18 | The Children's Hospital Of Philadelphia | Biodegradable linkers for molecular therapies |
| CN101534643A (zh) | 2006-09-15 | 2009-09-16 | 安佐制药股份有限公司 | 用于递送寡核苷酸的基于位阻酯的生物可降解连接体 |
| JP5637857B2 (ja) * | 2008-10-28 | 2014-12-10 | 学校法人関西学院 | リフォールディング剤および蛋白質のリフォールディング方法 |
| CA2873452A1 (en) | 2012-05-16 | 2013-11-21 | Noxxon Pharma Ag | Enzymatic synthesis of l-nucleic acids |
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- 2013-05-16 RU RU2014140243A patent/RU2704833C2/ru active
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- 2013-05-16 WO PCT/EP2013/001458 patent/WO2013170963A2/en not_active Ceased
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2014
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998008856A2 (de) * | 1996-08-30 | 1998-03-05 | Fuerste Jens Peter | Spiegelselektion und spiegelevolution von nucleinsäuren |
| WO2003020969A2 (de) * | 2001-08-30 | 2003-03-13 | Noxxon Pharma Ag | Verfahren zur markierung von l-nukleinsäuren |
| WO2003035665A1 (de) * | 2001-10-26 | 2003-05-01 | Noxxon Pharma Ag | Modifizierte l-nukleinsäure |
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| KEEFE A.D. ET AL. Aptamers as therapeutics // NATURE REVIEWS, Drug Discovery, JULY 2010, volume 9, 537-550. * |
| KEEFE A.D. ET AL. Aptamers as therapeutics // NATURE REVIEWS, Drug Discovery, JULY 2010, volume 9, 537-550. Биохимия: Учебник / Под ред. Е.С. Северина. - 2-е изд., испр. - М.: ГЭОТАР-МЕД, 2004, с. 151-153. * |
| Биохимия: Учебник / Под ред. Е.С. Северина. - 2-е изд., испр. - М.: ГЭОТАР-МЕД, 2004, с. 151-153. * |
Also Published As
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| JP6522496B2 (ja) | 2019-05-29 |
| EP2850192B1 (en) | 2023-11-01 |
| KR102168915B1 (ko) | 2020-10-23 |
| AU2013262069C1 (en) | 2019-01-31 |
| EP2850192A2 (en) | 2015-03-25 |
| IL235556B (en) | 2020-02-27 |
| EP2850192C0 (en) | 2023-11-01 |
| CN104379748A (zh) | 2015-02-25 |
| ES2965777T3 (es) | 2024-04-16 |
| US11015178B2 (en) | 2021-05-25 |
| CN109554418A (zh) | 2019-04-02 |
| CN104379748B (zh) | 2019-01-04 |
| IL235556A0 (en) | 2015-01-29 |
| JP2015516165A (ja) | 2015-06-11 |
| US20180208910A1 (en) | 2018-07-26 |
| US9850471B2 (en) | 2017-12-26 |
| AU2013262069B2 (en) | 2018-04-05 |
| CA2873452A1 (en) | 2013-11-21 |
| WO2013170963A3 (en) | 2014-01-23 |
| RU2014140243A (ru) | 2016-07-10 |
| KR20150013804A (ko) | 2015-02-05 |
| WO2013170963A2 (en) | 2013-11-21 |
| AU2013262069A1 (en) | 2014-10-23 |
| US20150337348A1 (en) | 2015-11-26 |
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