JP2015154787A5 - - Google Patents

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JP2015154787A5
JP2015154787A5 JP2015111259A JP2015111259A JP2015154787A5 JP 2015154787 A5 JP2015154787 A5 JP 2015154787A5 JP 2015111259 A JP2015111259 A JP 2015111259A JP 2015111259 A JP2015111259 A JP 2015111259A JP 2015154787 A5 JP2015154787 A5 JP 2015154787A5
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JP
Japan
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polymerase
seq
amino acid
domain
chimeric
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JP2015111259A
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Japanese (ja)
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JP2015154787A (ja
JP6150847B2 (ja
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JP2015111259A 2008-11-03 2015-06-01 キメラdnaポリメラーゼ Active JP6150847B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11086208P 2008-11-03 2008-11-03
US61/110,862 2008-11-03

Related Parent Applications (1)

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JP2011534889A Division JP2012507986A (ja) 2008-11-03 2009-11-03 キメラdnaポリメラーゼ

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016219403A Division JP2017029172A (ja) 2008-11-03 2016-11-10 キメラdnaポリメラーゼ

Publications (3)

Publication Number Publication Date
JP2015154787A JP2015154787A (ja) 2015-08-27
JP2015154787A5 true JP2015154787A5 (https=) 2016-02-18
JP6150847B2 JP6150847B2 (ja) 2017-06-21

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ID=42226349

Family Applications (3)

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JP2011534889A Withdrawn JP2012507986A (ja) 2008-11-03 2009-11-03 キメラdnaポリメラーゼ
JP2015111259A Active JP6150847B2 (ja) 2008-11-03 2015-06-01 キメラdnaポリメラーゼ
JP2016219403A Pending JP2017029172A (ja) 2008-11-03 2016-11-10 キメラdnaポリメラーゼ

Family Applications Before (1)

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JP2011534889A Withdrawn JP2012507986A (ja) 2008-11-03 2009-11-03 キメラdnaポリメラーゼ

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2016219403A Pending JP2017029172A (ja) 2008-11-03 2016-11-10 キメラdnaポリメラーゼ

Country Status (7)

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US (2) US9023633B2 (https=)
EP (2) EP2927317B1 (https=)
JP (3) JP2012507986A (https=)
CN (1) CN102257136A (https=)
CA (1) CA2742593C (https=)
ES (2) ES2716402T3 (https=)
WO (1) WO2010062776A2 (https=)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010062777A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Modified type a dna polymerases
CA2742593C (en) 2008-11-03 2020-05-05 Kapa Biosystems, Inc. Chimeric dna polymerases
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
CN102392000A (zh) * 2011-11-07 2012-03-28 李志茹 一种耐高温Pyrolobus聚合酶及其高效表达质粒和应用
TWI487789B (zh) 2012-12-24 2015-06-11 Ind Tech Res Inst 單離的脫氧核糖核酸聚合酶、套組與其應用
CN104250641B (zh) * 2013-06-28 2017-09-01 北京福安华生物科技有限公司 一种高保真dna聚合酶及其制备和应用
JP6428997B2 (ja) * 2013-08-06 2018-11-28 東洋紡株式会社 核酸増幅法
EP3702471B1 (en) * 2014-06-27 2025-12-24 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
EP3778891A1 (en) * 2014-08-27 2021-02-17 New England Biolabs, Inc. Synthon formation
US10329545B2 (en) 2014-11-14 2019-06-25 Illumina, Inc. Polymerases
JP6619152B2 (ja) * 2015-05-08 2019-12-11 国立研究開発法人産業技術総合研究所 フラビンアデニンジヌクレオチド依存型グルコース脱水素酵素活性を有するキメラタンパク質
CN106867978A (zh) * 2015-12-10 2017-06-20 江苏众红生物工程创药研究院有限公司 一种重组高保真dna聚合酶及其编码基因和表达方法
JP6969089B2 (ja) * 2015-12-11 2021-11-24 東洋紡株式会社 核酸増幅用組成物及び核酸増幅法
JP6980375B2 (ja) * 2015-12-11 2021-12-15 東洋紡株式会社 核酸増幅用試薬及び核酸増幅法
WO2017121836A1 (en) 2016-01-15 2017-07-20 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
JP6934477B2 (ja) 2016-01-19 2021-09-15 ボード オブ リージェンツ ザ ユニヴァーシティ オブ テキサス システム 熱安定逆転写酵素
US20190176153A1 (en) 2016-05-18 2019-06-13 Roche Sequencing Solutions, Inc. Quantitative real time pcr amplification using an electrowetting-based device
FR3052462A1 (fr) * 2016-06-14 2017-12-15 Dna Script Variants d'une adn polymerase de la famille polx
CN106011100A (zh) * 2016-08-08 2016-10-12 吴江近岸蛋白质科技有限公司 一种Pfu DNA聚合酶及其制备方法
WO2018118997A2 (en) 2016-12-19 2018-06-28 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
CN106754816B (zh) * 2017-02-24 2020-10-27 依科赛生物科技(太仓)有限公司 一种高保真快速扩增融合酶及其制备方法
EP3645710A1 (en) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
KR20250028535A (ko) 2017-06-30 2025-02-28 코덱시스, 인코포레이티드 T7 rna 폴리머라제 변이체
EP3645712A4 (en) 2017-06-30 2021-07-07 Codexis, Inc. T7 RNA POLYMERASE VARIANTS
EP3653729B1 (en) * 2017-07-12 2025-10-15 Genecast Co., Ltd. Dna polymerase with increased gene mutation specificity and pcr buffer composition for increasing activity thereof
WO2019084538A1 (en) 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF
US10961518B2 (en) 2018-01-19 2021-03-30 Bio-Rad Laboratories, Inc. Mutant DNA polymerases
CN112639089B (zh) * 2018-09-03 2024-02-09 深圳华大生命科学研究院 重组型kod聚合酶
EP3874036A1 (en) * 2018-10-31 2021-09-08 Illumina, Inc. Polymerases, compositions, and methods of use
WO2020117968A2 (en) 2018-12-05 2020-06-11 Illumina, Inc. Polymerases, compositions, and methods of use
CN109628424B (zh) * 2018-12-31 2022-04-26 苏州近岸蛋白质科技股份有限公司 一种新型嵌合dna聚合酶及其制备方法
GB2587177A (en) * 2019-01-09 2021-03-24 3Cr Bioscience Ltd Polymerase
US20220090194A1 (en) * 2019-01-09 2022-03-24 3Cr Bioscience Ltd. Method for detecting a nucleic acid sequence
US11959105B2 (en) 2019-06-28 2024-04-16 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
US20240200040A1 (en) * 2019-12-23 2024-06-20 Bgi Shenzhen Chimeric dna polymerase and application thereof
US20230086471A1 (en) * 2020-02-15 2023-03-23 Agency For Science, Technology And Research A method to amplify a nucleic acid
CN113604450B (zh) * 2021-08-18 2023-08-18 华南理工大学 一种kod dna聚合酶突变体及其制备方法与应用
CN113755465A (zh) * 2021-09-23 2021-12-07 武汉爱博泰克生物科技有限公司 嵌合体dna聚合酶及其制备方法
US20250327044A1 (en) * 2021-11-15 2025-10-23 Bgi Shenzhen Chimeric dna polymerase and use thereof
CN119736271B (zh) * 2024-12-27 2026-03-20 安序源生物科技(深圳)有限公司 一种phi29 DNA聚合酶突变体及其用途

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5091310A (en) 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5075216A (en) 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5866395A (en) 1990-12-03 1999-02-02 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5545552A (en) 1990-12-03 1996-08-13 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5948663A (en) 1990-12-03 1999-09-07 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
DE547359T1 (de) 1991-12-18 1993-11-25 New England Biolabs Inc Gereinigte thermostabile DNS-Polymerase aus Pyrococcus-Arten.
US5556772A (en) 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
JP3132624B2 (ja) 1994-05-09 2001-02-05 東洋紡績株式会社 超好熱始原菌由来のdnaポリメラーゼ遺伝子およびその用途
US6033859A (en) 1996-05-24 2000-03-07 Toyo Boseki Kabushiki Kaisha Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1
WO1998033900A1 (en) 1997-01-31 1998-08-06 Japan Science And Technology Corporation Method and apparatus for predicting protein function site, method for improving protein function, and function-improved protein
DE19810879A1 (de) * 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
DE69943048D1 (de) 1998-08-18 2011-01-27 Dermtech International San Diego Methode zum nachweis biologischer faktoren in der epidermis
GB9921318D0 (en) * 1999-09-09 1999-11-10 Kristensen Tom Chimeric molecules
US20050123940A1 (en) 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
DE60027040T2 (de) 1999-10-29 2006-11-23 Stratagene California, La Jolla Zusammensetzungen und methoden zur verwendung von dna polymerasen
ATE491031T1 (de) * 2000-02-17 2010-12-15 Qiagen Gmbh Thermostabile chimäre nukleinsäurepolymerasen und ihre verwendungen
DE60141097D1 (de) 2000-05-11 2010-03-11 Toyo Boseki Modifizierte thermostabile dna polymerase von pyrococcus kodakaraensis
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
EP1301205A1 (en) * 2000-07-12 2003-04-16 Invitrogen Corporation High fidelity polymerases and uses thereof
GB2382321A (en) 2001-11-21 2003-05-28 Doyen Medipharm Ltd Process and apparatus for cutting sheets
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
AU2004225520A1 (en) 2003-03-25 2004-10-14 Stratagene DNA polymerase fusions and uses thereof
US20040244310A1 (en) 2003-03-28 2004-12-09 Blumberg Marvin R. Data center
AU2005245966B2 (en) * 2004-05-20 2010-04-01 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Thermostable Y-family polymerases and chimeras
EP1888743B1 (en) 2005-05-10 2011-08-03 Illumina Cambridge Limited Improved polymerases
US20070190538A1 (en) 2005-07-29 2007-08-16 Applera Corporation Chimeric polymerases
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
JP2007298879A (ja) 2006-05-02 2007-11-15 C & C:Kk ストロボ装置
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
CA2742593C (en) 2008-11-03 2020-05-05 Kapa Biosystems, Inc. Chimeric dna polymerases
US8481685B2 (en) 2008-11-03 2013-07-09 Kapa Biosystems Modified DNA polymerases
JP5328696B2 (ja) 2010-03-01 2013-10-30 淀川ヒューテック株式会社 複層ベルト

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