DE547359T1 - Gereinigte thermostabile DNS-Polymerase aus Pyrococcus-Arten. - Google Patents

Gereinigte thermostabile DNS-Polymerase aus Pyrococcus-Arten.

Info

Publication number
DE547359T1
DE547359T1 DE1992118965 DE92118965T DE547359T1 DE 547359 T1 DE547359 T1 DE 547359T1 DE 1992118965 DE1992118965 DE 1992118965 DE 92118965 T DE92118965 T DE 92118965T DE 547359 T1 DE547359 T1 DE 547359T1
Authority
DE
Germany
Prior art keywords
dna polymerase
thermostable dna
pyrococcus species
purified thermostable
purified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE1992118965
Other languages
English (en)
Inventor
Donald G Comb
Rebecca Kucera
Francine Perler
Huimin Kong
William E Jack
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Publication of DE547359T1 publication Critical patent/DE547359T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
DE1992118965 1991-12-18 1992-11-05 Gereinigte thermostabile DNS-Polymerase aus Pyrococcus-Arten. Pending DE547359T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US80982291A 1991-12-18 1991-12-18

Publications (1)

Publication Number Publication Date
DE547359T1 true DE547359T1 (de) 1993-11-25

Family

ID=25202300

Family Applications (2)

Application Number Title Priority Date Filing Date
DE1992118965 Pending DE547359T1 (de) 1991-12-18 1992-11-05 Gereinigte thermostabile DNS-Polymerase aus Pyrococcus-Arten.
DE1992632511 Expired - Lifetime DE69232511T2 (de) 1991-12-18 1992-11-05 Gereinigte thermostabile DNS-Polymerase aus Pyrococcus-Arten

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE1992632511 Expired - Lifetime DE69232511T2 (de) 1991-12-18 1992-11-05 Gereinigte thermostabile DNS-Polymerase aus Pyrococcus-Arten

Country Status (3)

Country Link
EP (1) EP0547359B1 (de)
JP (1) JP3453397B2 (de)
DE (2) DE547359T1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
EP0547920B1 (de) * 1991-12-18 2002-02-27 New England Biolabs, Inc. Rekombinante thermostabile DNA-Polymerase von Archaebakterie
US6100078A (en) * 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
CN1123328A (zh) * 1994-11-17 1996-05-29 中国科学院上海生物化学研究所 高温、高传真脱氧核糖核酸(DNA)聚合酶(HiFi Bst)
CA2241170A1 (en) 1995-12-27 1997-07-10 Takara Shuzo Co., Ltd. Novel dna polymerase
DE19611759A1 (de) * 1996-03-25 1997-10-02 Boehringer Mannheim Gmbh Thermostabile DNA-Polymerase aus Thermococcus spec.
FR2750999B1 (fr) 1996-07-10 1998-11-20 Appligene Oncor Adn polymerase thermostable d'archaebacteries du genre pyrococcus sp
US5827716A (en) * 1996-07-30 1998-10-27 Amersham Life Science, Inc. Modified Pol-II type DNA polymerases
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US5882904A (en) * 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
DE60141097D1 (de) 2000-05-11 2010-03-11 Toyo Boseki Modifizierte thermostabile dna polymerase von pyrococcus kodakaraensis
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
EP2208796B1 (de) 2005-01-06 2014-06-18 Applied Biosystems, LLC Polypeptide mit Nukleinsäurebindungsaktivität und Zusammensetzungen und Verfahren zur Nukleinsäurenamplifikation
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US9023633B2 (en) * 2008-11-03 2015-05-05 Kapa Biosystems Chimeric DNA polymerases

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5322785A (en) * 1990-04-26 1994-06-21 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
WO1992009689A1 (en) * 1990-12-03 1992-06-11 Stratagene PURIFIED THERMOSTABLE $i(PYROCOCCUS FURIOSUS)

Also Published As

Publication number Publication date
JP3453397B2 (ja) 2003-10-06
EP0547359A1 (de) 1993-06-23
JPH05328969A (ja) 1993-12-14
DE69232511D1 (de) 2002-05-02
EP0547359B1 (de) 2002-03-27
DE69232511T2 (de) 2002-10-24

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