DE69527803D1 - Aufgereinigte dna-polymerase von thermococcus barossii - Google Patents

Aufgereinigte dna-polymerase von thermococcus barossii

Info

Publication number
DE69527803D1
DE69527803D1 DE69527803T DE69527803T DE69527803D1 DE 69527803 D1 DE69527803 D1 DE 69527803D1 DE 69527803 T DE69527803 T DE 69527803T DE 69527803 T DE69527803 T DE 69527803T DE 69527803 D1 DE69527803 D1 DE 69527803D1
Authority
DE
Germany
Prior art keywords
dna polymerase
purified dna
thermococcus barossii
barossii
thermococcus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69527803T
Other languages
English (en)
Other versions
DE69527803T2 (de
Inventor
A Luhm
Hennezel B D
D Duffaud
F Jolly
M Kelly
Yi-Fay Ting
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
North Carolina State University
University of California
Global Life Sciences Solutions USA LLC
Original Assignee
North Carolina State University
University of California
Pharmacia Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by North Carolina State University, University of California, Pharmacia Biotech Inc filed Critical North Carolina State University
Publication of DE69527803D1 publication Critical patent/DE69527803D1/de
Application granted granted Critical
Publication of DE69527803T2 publication Critical patent/DE69527803T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69527803T 1995-01-18 1995-11-29 Aufgereinigte dna-polymerase von thermococcus barossii Expired - Fee Related DE69527803T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/375,134 US5602011A (en) 1995-01-18 1995-01-18 Purified Thermococcus barossii DNA polymerase
PCT/US1995/015263 WO1996022389A1 (en) 1995-01-18 1995-11-29 Purified thermococcus barossii dna polymerase

Publications (2)

Publication Number Publication Date
DE69527803D1 true DE69527803D1 (de) 2002-09-19
DE69527803T2 DE69527803T2 (de) 2003-04-24

Family

ID=23479638

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69527803T Expired - Fee Related DE69527803T2 (de) 1995-01-18 1995-11-29 Aufgereinigte dna-polymerase von thermococcus barossii

Country Status (7)

Country Link
US (1) US5602011A (de)
EP (1) EP0799319B1 (de)
JP (1) JPH10503381A (de)
AU (1) AU692025B2 (de)
CA (1) CA2210895A1 (de)
DE (1) DE69527803T2 (de)
WO (1) WO1996022389A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19611759A1 (de) * 1996-03-25 1997-10-02 Boehringer Mannheim Gmbh Thermostabile DNA-Polymerase aus Thermococcus spec.
EP0822256B1 (de) * 1996-07-29 2003-09-24 Toyo Boseki Kabushiki Kaisha Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
US5882904A (en) * 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
US20040214221A1 (en) * 1999-05-07 2004-10-28 Klaus Muehlegger High density labeling of DNA with modified or "chromophore" carrying nucleotides and DNA polymerases used
US20050123940A1 (en) * 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
US20040009486A1 (en) * 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
WO2001061015A2 (de) * 2000-02-17 2001-08-23 Qiagen Gmbh Thermostable chimeric nucleic acid polymerases and uses thereof
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
US20030134292A1 (en) * 2001-10-30 2003-07-17 Farchaus Joseph W. Thermostable DNA polymerases and methods of making same
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
EP1606419A1 (de) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systeme und methoden zur erhohung der milchproduktion in tieren
CN1913841B (zh) 2004-01-27 2014-06-11 日本来富恩株式会社 烧蚀导管
US20050202484A1 (en) 2004-02-19 2005-09-15 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
BRPI0515777A (pt) * 2004-12-11 2008-08-05 Cytogenix Inc biossìntese isenta de células de ácido nucléico de alta qualidade e usos dos mesmos
KR100777230B1 (ko) * 2006-11-30 2007-11-28 한국해양연구원 써모코커스 유래 돌연변이 ｄｎａ 중합효소들 및 그의유전자들
JP2010528669A (ja) * 2007-06-13 2010-08-26 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション ポリメラーゼ安定化
WO2009085333A1 (en) * 2007-12-27 2009-07-09 Ge Healthcare Bio-Sciences Corp. A single enzyme system for fast, ultra long pcr
KR20170053636A (ko) * 2014-09-11 2017-05-16 다카라 바이오 가부시키가이샤 내열성의 미스매치 엔도뉴클레아제의 이용방법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5352600A (en) * 1986-08-22 1994-10-04 Hoffmann-La Roche Inc. Purified thermostable enzyme
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
JP2531246B2 (ja) * 1988-08-26 1996-09-04 東洋紡績株式会社 耐熱性ｄｎａポリメラ―ゼおよびその製法
US5322785A (en) * 1990-04-26 1994-06-21 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
JPH067160A (ja) * 1991-12-18 1994-01-18 New England Biolabs Inc 古細菌からの組換え熱安定性ｄｎａポリメラーゼ

Also Published As

Publication number Publication date
JPH10503381A (ja) 1998-03-31
EP0799319A1 (de) 1997-10-08
CA2210895A1 (en) 1996-07-25
AU4287196A (en) 1996-08-07
AU692025B2 (en) 1998-05-28
EP0799319B1 (de) 2002-08-14
WO1996022389A1 (en) 1996-07-25
US5602011A (en) 1997-02-11
DE69527803T2 (de) 2003-04-24
EP0799319A4 (de) 1999-06-30

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Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee
8364 No opposition during term of opposition