ES2716402T3 - ADN polimerasas quiméricas - Google Patents

ADN polimerasas quiméricas Download PDF

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Publication number
ES2716402T3
ES2716402T3 ES15160891T ES15160891T ES2716402T3 ES 2716402 T3 ES2716402 T3 ES 2716402T3 ES 15160891 T ES15160891 T ES 15160891T ES 15160891 T ES15160891 T ES 15160891T ES 2716402 T3 ES2716402 T3 ES 2716402T3
Authority
ES
Spain
Prior art keywords
dna
polymerase
polymerases
seq
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES15160891T
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English (en)
Spanish (es)
Inventor
William Bourn
Gavin Rush
Paul Mcewan
Bjarne Faurholm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kapa Biosystems Inc
Original Assignee
Kapa Biosystems Inc
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Publication of ES2716402T3 publication Critical patent/ES2716402T3/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES15160891T 2008-11-03 2009-11-03 ADN polimerasas quiméricas Active ES2716402T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11086208P 2008-11-03 2008-11-03

Publications (1)

Publication Number Publication Date
ES2716402T3 true ES2716402T3 (es) 2019-06-12

Family

ID=42226349

Family Applications (2)

Application Number Title Priority Date Filing Date
ES15160891T Active ES2716402T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas
ES09829689T Active ES2571446T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES09829689T Active ES2571446T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas

Country Status (7)

Country Link
US (2) US9023633B2 (https=)
EP (2) EP2927317B1 (https=)
JP (3) JP2012507986A (https=)
CN (1) CN102257136A (https=)
CA (1) CA2742593C (https=)
ES (2) ES2716402T3 (https=)
WO (1) WO2010062776A2 (https=)

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CN104250641B (zh) * 2013-06-28 2017-09-01 北京福安华生物科技有限公司 一种高保真dna聚合酶及其制备和应用
JP6428997B2 (ja) * 2013-08-06 2018-11-28 東洋紡株式会社 核酸増幅法
EP3702471B1 (en) * 2014-06-27 2025-12-24 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
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CN106867978A (zh) * 2015-12-10 2017-06-20 江苏众红生物工程创药研究院有限公司 一种重组高保真dna聚合酶及其编码基因和表达方法
JP6969089B2 (ja) * 2015-12-11 2021-11-24 東洋紡株式会社 核酸増幅用組成物及び核酸増幅法
JP6980375B2 (ja) * 2015-12-11 2021-12-15 東洋紡株式会社 核酸増幅用試薬及び核酸増幅法
WO2017121836A1 (en) 2016-01-15 2017-07-20 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
JP6934477B2 (ja) 2016-01-19 2021-09-15 ボード オブ リージェンツ ザ ユニヴァーシティ オブ テキサス システム 熱安定逆転写酵素
US20190176153A1 (en) 2016-05-18 2019-06-13 Roche Sequencing Solutions, Inc. Quantitative real time pcr amplification using an electrowetting-based device
FR3052462A1 (fr) * 2016-06-14 2017-12-15 Dna Script Variants d'une adn polymerase de la famille polx
CN106011100A (zh) * 2016-08-08 2016-10-12 吴江近岸蛋白质科技有限公司 一种Pfu DNA聚合酶及其制备方法
WO2018118997A2 (en) 2016-12-19 2018-06-28 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
CN106754816B (zh) * 2017-02-24 2020-10-27 依科赛生物科技（太仓）有限公司 一种高保真快速扩增融合酶及其制备方法
EP3645710A1 (en) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
KR20250028535A (ko) 2017-06-30 2025-02-28 코덱시스, 인코포레이티드 T7 rna 폴리머라제 변이체
EP3645712A4 (en) 2017-06-30 2021-07-07 Codexis, Inc. T7 RNA POLYMERASE VARIANTS
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WO2019084538A1 (en) 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF
US10961518B2 (en) 2018-01-19 2021-03-30 Bio-Rad Laboratories, Inc. Mutant DNA polymerases
CN112639089B (zh) * 2018-09-03 2024-02-09 深圳华大生命科学研究院 重组型kod聚合酶
EP3874036A1 (en) * 2018-10-31 2021-09-08 Illumina, Inc. Polymerases, compositions, and methods of use
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CN109628424B (zh) * 2018-12-31 2022-04-26 苏州近岸蛋白质科技股份有限公司 一种新型嵌合dna聚合酶及其制备方法
GB2587177A (en) * 2019-01-09 2021-03-24 3Cr Bioscience Ltd Polymerase
US20220090194A1 (en) * 2019-01-09 2022-03-24 3Cr Bioscience Ltd. Method for detecting a nucleic acid sequence
US11959105B2 (en) 2019-06-28 2024-04-16 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
US20240200040A1 (en) * 2019-12-23 2024-06-20 Bgi Shenzhen Chimeric dna polymerase and application thereof
US20230086471A1 (en) * 2020-02-15 2023-03-23 Agency For Science, Technology And Research A method to amplify a nucleic acid
CN113604450B (zh) * 2021-08-18 2023-08-18 华南理工大学 一种kod dna聚合酶突变体及其制备方法与应用
CN113755465A (zh) * 2021-09-23 2021-12-07 武汉爱博泰克生物科技有限公司 嵌合体dna聚合酶及其制备方法
US20250327044A1 (en) * 2021-11-15 2025-10-23 Bgi Shenzhen Chimeric dna polymerase and use thereof
CN119736271B (zh) * 2024-12-27 2026-03-20 安序源生物科技(深圳)有限公司 一种phi29 DNA聚合酶突变体及其用途

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Also Published As

Publication number Publication date
HK1216032A1 (en) 2016-10-07
US20140363848A1 (en) 2014-12-11
EP2927317B1 (en) 2018-12-19
ES2571446T3 (es) 2016-05-25
US9388396B2 (en) 2016-07-12
US20120115188A1 (en) 2012-05-10
EP2927317A1 (en) 2015-10-07
EP2352818A2 (en) 2011-08-10
JP2015154787A (ja) 2015-08-27
JP2017029172A (ja) 2017-02-09
EP2352818A4 (en) 2011-10-12
CN102257136A (zh) 2011-11-23
WO2010062776A3 (en) 2010-08-19
EP2352818B1 (en) 2016-02-10
JP2012507986A (ja) 2012-04-05
CA2742593C (en) 2020-05-05
WO2010062776A2 (en) 2010-06-03
CA2742593A1 (en) 2010-06-03
JP6150847B2 (ja) 2017-06-21
US9023633B2 (en) 2015-05-05

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