CA2742593C - Chimeric dna polymerases - Google Patents

Chimeric dna polymerases Download PDF

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Publication number
CA2742593C
CA2742593C CA2742593A CA2742593A CA2742593C CA 2742593 C CA2742593 C CA 2742593C CA 2742593 A CA2742593 A CA 2742593A CA 2742593 A CA2742593 A CA 2742593A CA 2742593 C CA2742593 C CA 2742593C
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polymerase
dna
sequence
domain
seq
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CA2742593A
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English (en)
French (fr)
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CA2742593A1 (en
Inventor
Bjarne Faurholm
Paul McEwan
William Bourn
Gavin Rush
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Kapa Biosystems Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2742593A 2008-11-03 2009-11-03 Chimeric dna polymerases Active CA2742593C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11086208P 2008-11-03 2008-11-03
US61/110,862 2008-11-03
PCT/US2009/063166 WO2010062776A2 (en) 2008-11-03 2009-11-03 Chimeric dna polymerases

Publications (2)

Publication Number Publication Date
CA2742593A1 CA2742593A1 (en) 2010-06-03
CA2742593C true CA2742593C (en) 2020-05-05

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ID=42226349

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2742593A Active CA2742593C (en) 2008-11-03 2009-11-03 Chimeric dna polymerases

Country Status (7)

Country Link
US (2) US9023633B2 (https=)
EP (2) EP2927317B1 (https=)
JP (3) JP2012507986A (https=)
CN (1) CN102257136A (https=)
CA (1) CA2742593C (https=)
ES (2) ES2716402T3 (https=)
WO (1) WO2010062776A2 (https=)

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CN109628424B (zh) * 2018-12-31 2022-04-26 苏州近岸蛋白质科技股份有限公司 一种新型嵌合dna聚合酶及其制备方法
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US20240200040A1 (en) * 2019-12-23 2024-06-20 Bgi Shenzhen Chimeric dna polymerase and application thereof
US20230086471A1 (en) * 2020-02-15 2023-03-23 Agency For Science, Technology And Research A method to amplify a nucleic acid
CN113604450B (zh) * 2021-08-18 2023-08-18 华南理工大学 一种kod dna聚合酶突变体及其制备方法与应用
CN113755465A (zh) * 2021-09-23 2021-12-07 武汉爱博泰克生物科技有限公司 嵌合体dna聚合酶及其制备方法
US20250327044A1 (en) * 2021-11-15 2025-10-23 Bgi Shenzhen Chimeric dna polymerase and use thereof
CN119736271B (zh) * 2024-12-27 2026-03-20 安序源生物科技(深圳)有限公司 一种phi29 DNA聚合酶突变体及其用途

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Also Published As

Publication number Publication date
HK1216032A1 (en) 2016-10-07
US20140363848A1 (en) 2014-12-11
EP2927317B1 (en) 2018-12-19
ES2571446T3 (es) 2016-05-25
US9388396B2 (en) 2016-07-12
US20120115188A1 (en) 2012-05-10
EP2927317A1 (en) 2015-10-07
EP2352818A2 (en) 2011-08-10
JP2015154787A (ja) 2015-08-27
JP2017029172A (ja) 2017-02-09
EP2352818A4 (en) 2011-10-12
ES2716402T3 (es) 2019-06-12
CN102257136A (zh) 2011-11-23
WO2010062776A3 (en) 2010-08-19
EP2352818B1 (en) 2016-02-10
JP2012507986A (ja) 2012-04-05
WO2010062776A2 (en) 2010-06-03
CA2742593A1 (en) 2010-06-03
JP6150847B2 (ja) 2017-06-21
US9023633B2 (en) 2015-05-05

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