JP2008506417A5 - - Google Patents

Info

Publication number

JP2008506417A5

JP2008506417A5 JP2007527461A JP2007527461A JP2008506417A5 JP 2008506417 A5 JP2008506417 A5 JP 2008506417A5 JP 2007527461 A JP2007527461 A JP 2007527461A JP 2007527461 A JP2007527461 A JP 2007527461A JP 2008506417 A5 JP2008506417 A5 JP 2008506417A5

Authority

JP

Japan

Prior art keywords

seq

polymerase

blood

volume

resistant

Prior art date

2005-05-20

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

• Espacenet
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• 239000008280 blood Substances 0.000 claims 37
• 210000004369 blood Anatomy 0.000 claims 37
• 238000000034 method Methods 0.000 claims 35
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 17
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 17
• 230000004544 DNA amplification Effects 0.000 claims 16
• 108020004707 nucleic acids Proteins 0.000 claims 16
• 102000039446 nucleic acids Human genes 0.000 claims 16
• 150000007523 nucleic acids Chemical class 0.000 claims 16
• 238000006243 chemical reaction Methods 0.000 claims 10
• 229920001184 polypeptide Polymers 0.000 claims 10
• 102000004196 processed proteins & peptides Human genes 0.000 claims 10
• 108090000765 processed proteins & peptides Proteins 0.000 claims 10
• 238000006467 substitution reaction Methods 0.000 claims 8
• 238000002944 PCR assay Methods 0.000 claims 7
• 108020004705 Codon Proteins 0.000 claims 6
• 125000000539 amino acid group Chemical group 0.000 claims 6
• 239000000203 mixture Substances 0.000 claims 6
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 4
• 108010006785 Taq Polymerase Proteins 0.000 claims 4
• 125000003275 alpha amino acid group Chemical group 0.000 claims 4
• 230000000694 effects Effects 0.000 claims 4
• 239000002773 nucleotide Substances 0.000 claims 4
• 125000003729 nucleotide group Chemical group 0.000 claims 4
• 108010043461 Deep Vent DNA polymerase Proteins 0.000 claims 2
• 108010002747 Pfu DNA polymerase Proteins 0.000 claims 2
• 101000865057 Thermococcus litoralis DNA polymerase Proteins 0.000 claims 2
• 108010068698 spleen exonuclease Proteins 0.000 claims 2
• KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims 1
• FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims 1
• FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims 1
• 108020004414 DNA Proteins 0.000 claims 1
• KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims 1
• 108091036333 Rapid DNA Proteins 0.000 claims 1
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 1
• 229930006000 Sucrose Natural products 0.000 claims 1
• 229960003237 betaine Drugs 0.000 claims 1
• 230000001351 cycling effect Effects 0.000 claims 1
• 159000000003 magnesium salts Chemical class 0.000 claims 1
• 239000000600 sorbitol Substances 0.000 claims 1
• 239000005720 sucrose Substances 0.000 claims 1
• 238000005382 thermal cycling Methods 0.000 claims 1
• 239000001226 triphosphate Substances 0.000 claims 1
• 235000011178 triphosphate Nutrition 0.000 claims 1
• UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1