JP2008516584A5 - - Google Patents
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- Publication number
- JP2008516584A5 JP2008516584A5 JP2007519541A JP2007519541A JP2008516584A5 JP 2008516584 A5 JP2008516584 A5 JP 2008516584A5 JP 2007519541 A JP2007519541 A JP 2007519541A JP 2007519541 A JP2007519541 A JP 2007519541A JP 2008516584 A5 JP2008516584 A5 JP 2008516584A5
- Authority
- JP
- Japan
- Prior art keywords
- temperature
- nucleic acid
- reaction mixture
- binding protein
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 37
- 239000011541 reaction mixture Substances 0.000 claims description 22
- 150000007523 nucleic acids Chemical class 0.000 claims description 17
- 101710088729 Single-stranded nucleic acid-binding protein Proteins 0.000 claims description 14
- 108020004707 nucleic acids Proteins 0.000 claims description 14
- 102000039446 nucleic acids Human genes 0.000 claims description 14
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 238000009396 hybridization Methods 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 3
- 230000000295 complement effect Effects 0.000 claims description 3
- 230000003362 replicative effect Effects 0.000 claims description 3
- 230000001404 mediated effect Effects 0.000 claims 5
- 238000001668 nucleic acid synthesis Methods 0.000 claims 5
- 239000002773 nucleotide Substances 0.000 claims 5
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 3
- 239000000203 mixture Substances 0.000 claims 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims 3
- 239000007853 buffer solution Substances 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 150000001768 cations Chemical class 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 238000004925 denaturation Methods 0.000 claims 1
- 230000036425 denaturation Effects 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 239000011259 mixed solution Substances 0.000 claims 1
- 108700020942 nucleic acid binding protein Proteins 0.000 claims 1
- 102000044158 nucleic acid binding protein Human genes 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US58436204P | 2004-06-30 | 2004-06-30 | |
| US60/584,362 | 2004-06-30 | ||
| PCT/US2005/023824 WO2006005074A2 (en) | 2004-06-30 | 2005-06-30 | Novel hot start nucleic acid amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008516584A JP2008516584A (ja) | 2008-05-22 |
| JP2008516584A5 true JP2008516584A5 (https=) | 2009-07-23 |
| JP5020074B2 JP5020074B2 (ja) | 2012-09-05 |
Family
ID=35783411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007519541A Expired - Fee Related JP5020074B2 (ja) | 2004-06-30 | 2005-06-30 | 新規ホットスタート核酸増幅法 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US7700281B2 (https=) |
| EP (2) | EP2366802A1 (https=) |
| JP (1) | JP5020074B2 (https=) |
| AT (1) | ATE527380T1 (https=) |
| CA (1) | CA2569394A1 (https=) |
| NO (1) | NO20065470L (https=) |
| WO (1) | WO2006005074A2 (https=) |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7700281B2 (en) * | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
| ATE502122T1 (de) | 2006-06-01 | 2011-04-15 | Trilink Biotechnologies | Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation |
| WO2009029648A2 (en) | 2007-08-27 | 2009-03-05 | Applera Corporation | Methods and compositions for pcr |
| EP2391732B1 (en) | 2009-01-30 | 2015-05-27 | Oxford Nanopore Technologies Limited | Methods using adaptors for nucleic acid constructs in transmembrane sequencing |
| JP5687414B2 (ja) | 2009-04-20 | 2015-03-18 | オリンパス株式会社 | 多型の識別方法 |
| KR101162422B1 (ko) * | 2009-11-30 | 2012-07-10 | 박수민 | 핵산 증폭용 조성물 |
| EP2856974B1 (en) | 2011-04-06 | 2017-03-01 | DePuy Synthes Products, LLC | Distal reamer |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| EP2859111B1 (en) * | 2012-06-08 | 2020-07-08 | Ionian Technologies, LLC | Nucleic acid amplifications |
| JP6450308B2 (ja) | 2012-06-14 | 2019-01-09 | ライフ テクノロジーズ コーポレーション | ポリメラーゼ連鎖反応（ｐｃｒ）のための新規の組成物、方法、およびキット |
| US11155860B2 (en) * | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR20200140929A (ko) | 2013-02-08 | 2020-12-16 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| BR112016023625A2 (pt) | 2014-04-10 | 2018-06-26 | 10X Genomics, Inc. | dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| BR112017008877A2 (pt) | 2014-10-29 | 2018-07-03 | 10X Genomics Inc | métodos e composições para sequenciamento de ácido nucleico-alvo |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| JP6769969B2 (ja) | 2015-01-12 | 2020-10-14 | １０エックス ジェノミクス， インコーポレイテッド | 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー |
| MX2017010857A (es) | 2015-02-24 | 2017-12-11 | 10X Genomics Inc | Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana. |
| EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| JP6954899B2 (ja) | 2015-12-04 | 2021-10-27 | １０エックス ゲノミクス，インコーポレイテッド | 核酸の解析のための方法及び組成物 |
| WO2017121836A1 (en) | 2016-01-15 | 2017-07-20 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| EP3445876B1 (en) | 2017-05-26 | 2023-07-05 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| KR102724054B1 (ko) | 2017-06-07 | 2024-10-29 | 다카라 바이오 가부시키가이샤 | 올리고뉴클레오타이드의 보존 방법 |
| EP3645710A1 (en) | 2017-06-26 | 2020-05-06 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| CN112262218B (zh) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| KR102529958B1 (ko) * | 2021-01-18 | 2023-05-08 | 가톨릭대학교 산학협력단 | 저 gc 함량 dna의 정량적 증폭을 위한 pcr 첨가제 조성물 |
| CN116004776A (zh) * | 2022-08-30 | 2023-04-25 | 上海伯杰医疗科技股份有限公司 | 一管式RT-qPCR全预混反应试剂、应用及RT-qPCR方法 |
| WO2025071292A1 (ko) * | 2023-09-26 | 2025-04-03 | 주식회사 씨젠 | 핫 스타트 핵산 중합효소의 성능을 평가하는 방법 |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1985005685A1 (en) | 1984-06-01 | 1985-12-19 | National Biomedical Research Foundation | Catalyzed nucleic acid hybridization using enzymatic reagent |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US4994372A (en) | 1987-01-14 | 1991-02-19 | President And Fellows Of Harvard College | DNA sequencing |
| US5449603A (en) * | 1989-10-24 | 1995-09-12 | Stratagene | Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| US5646019A (en) * | 1989-10-24 | 1997-07-08 | Stratagene | Method for producing primed nucleic acid templates |
| US5605824A (en) * | 1989-10-24 | 1997-02-25 | Stratagene | Composition for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| WO1991006679A1 (en) | 1989-10-24 | 1991-05-16 | Stratagene | An improved method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| DK0515506T3 (da) | 1990-02-16 | 2000-05-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af carcinogene humane papillomavirus |
| US5223414A (en) * | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| CA2074214C (en) | 1991-07-23 | 2000-02-22 | Will Bloch | Improvements in the in situ pcr |
| WO1993015226A1 (en) * | 1992-01-28 | 1993-08-05 | President And Fellows Of Harvard College | Method for nucleic acid hybridization using single-stranded dna binding protein |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5364790A (en) * | 1993-02-16 | 1994-11-15 | The Perkin-Elmer Corporation | In situ PCR amplification system |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| EP0869187A3 (en) | 1997-02-24 | 2001-02-28 | Becton, Dickinson and Company | Replication of nucleic acids using single-strand DNA binding proteins |
| US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
| US6183998B1 (en) | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
| GB9920194D0 (en) | 1999-08-27 | 1999-10-27 | Advanced Biotech Ltd | A heat-stable thermostable DNA polymerase for use in nucleic acid amplification |
| WO2003008624A2 (en) | 2001-07-15 | 2003-01-30 | Keck Graduate Institute | Nucleic acid amplification using nicking agents |
| US7399590B2 (en) * | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| EP1539209A2 (en) * | 2002-09-05 | 2005-06-15 | Invitrogen Corporation | Compositions and methods for synthesizing nucleic acids |
| CA2498764C (en) | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
| JP2004113108A (ja) * | 2002-09-26 | 2004-04-15 | Aisin Seiki Co Ltd | Ｄｎａ回収法 |
| JP2005110621A (ja) | 2003-10-10 | 2005-04-28 | Aisin Seiki Co Ltd | 核酸増幅方法及び核酸増幅用試薬キット |
| US7759057B2 (en) * | 2004-02-04 | 2010-07-20 | Ut-Battelle, Llc | Method for analyzing microbial communities |
| US7700281B2 (en) * | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
-
2005
- 2005-06-29 US US11/171,008 patent/US7700281B2/en active Active
- 2005-06-30 JP JP2007519541A patent/JP5020074B2/ja not_active Expired - Fee Related
- 2005-06-30 EP EP11163362A patent/EP2366802A1/en not_active Withdrawn
- 2005-06-30 AT AT05764400T patent/ATE527380T1/de not_active IP Right Cessation
- 2005-06-30 EP EP05764400A patent/EP1778866B1/en not_active Expired - Lifetime
- 2005-06-30 WO PCT/US2005/023824 patent/WO2006005074A2/en not_active Ceased
- 2005-06-30 CA CA002569394A patent/CA2569394A1/en not_active Abandoned
-
2006
- 2006-11-28 NO NO20065470A patent/NO20065470L/no not_active Application Discontinuation
-
2007
- 2007-11-14 US US11/939,820 patent/US7951534B2/en not_active Expired - Lifetime
-
2011
- 2011-03-29 US US13/074,752 patent/US8404443B2/en not_active Expired - Lifetime
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