CA2569394A1 - Novel hot start nucleic acid amplification - Google Patents
Novel hot start nucleic acid amplification Download PDFInfo
- Publication number
- CA2569394A1 CA2569394A1 CA002569394A CA2569394A CA2569394A1 CA 2569394 A1 CA2569394 A1 CA 2569394A1 CA 002569394 A CA002569394 A CA 002569394A CA 2569394 A CA2569394 A CA 2569394A CA 2569394 A1 CA2569394 A1 CA 2569394A1
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- CA
- Canada
- Prior art keywords
- nucleic acid
- binding protein
- stranded nucleic
- primer
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Physics & Mathematics (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US58436204P | 2004-06-30 | 2004-06-30 | |
| US60/584,362 | 2004-06-30 | ||
| PCT/US2005/023824 WO2006005074A2 (en) | 2004-06-30 | 2005-06-30 | Novel hot start nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2569394A1 true CA2569394A1 (en) | 2006-01-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002569394A Abandoned CA2569394A1 (en) | 2004-06-30 | 2005-06-30 | Novel hot start nucleic acid amplification |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US7700281B2 (https=) |
| EP (2) | EP2366802A1 (https=) |
| JP (1) | JP5020074B2 (https=) |
| AT (1) | ATE527380T1 (https=) |
| CA (1) | CA2569394A1 (https=) |
| NO (1) | NO20065470L (https=) |
| WO (1) | WO2006005074A2 (https=) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US7700281B2 (en) * | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
| AU2007268075C1 (en) | 2006-06-01 | 2013-03-07 | Trilink Biotechnologies | Chemically modified oligonucleotide primers for nucleic acid amplification |
| JP2010537637A (ja) | 2007-08-27 | 2010-12-09 | アプライド バイオシステムズ インコーポレイテッド | Ｐｃｒのための方法および組成物 |
| JP5843614B2 (ja) | 2009-01-30 | 2016-01-13 | オックスフォード ナノポア テクノロジーズ リミテッド | 膜貫通配列決定における核酸構築物のためのアダプター |
| JP5687414B2 (ja) * | 2009-04-20 | 2015-03-18 | オリンパス株式会社 | 多型の識別方法 |
| KR101162422B1 (ko) * | 2009-11-30 | 2012-07-10 | 박수민 | 핵산 증폭용 조성물 |
| US9597188B2 (en) | 2011-04-06 | 2017-03-21 | DePuy Synthes Products, Inc. | Version-replicating instrument and orthopaedic surgical procedure for using the same to implant a revision hip prosthesis |
| AU2012288629B2 (en) | 2011-07-25 | 2017-02-02 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
| CN114214394A (zh) * | 2012-06-08 | 2022-03-22 | 爱奥尼安技术公司 | 核苷酸扩增反应 |
| KR20210072137A (ko) | 2012-06-14 | 2021-06-16 | 라이프 테크놀로지스 코포레이션 | 폴리머라제 연쇄 반응 (pcr)을 위한 신규 조성물, 방법 및 키트 |
| US11155860B2 (en) * | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA3216609C (en) | 2012-08-14 | 2024-05-14 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2014093676A1 (en) | 2012-12-14 | 2014-06-19 | 10X Technologies, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2014214682B2 (en) | 2013-02-08 | 2018-07-26 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| KR102168813B1 (ko) | 2013-03-08 | 2020-10-22 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 효소 정지 방법 |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| WO2015157567A1 (en) | 2014-04-10 | 2015-10-15 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| MX2016016904A (es) | 2014-06-26 | 2017-03-27 | 10X Genomics Inc | Analisis de secuencias de acidos nucleicos. |
| CN113249435B (zh) | 2014-06-26 | 2024-09-03 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
| MX2017005267A (es) | 2014-10-29 | 2017-07-26 | 10X Genomics Inc | Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana. |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| SG11201705615UA (en) | 2015-01-12 | 2017-08-30 | 10X Genomics Inc | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| EP4286516A3 (en) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partition processing methods and systems |
| BR112017018054A2 (pt) | 2015-02-24 | 2018-07-24 | 10X Genomics Inc | métodos para a cobertura de sequências de ácidos nucleicos direcionadas |
| SG11201804086VA (en) | 2015-12-04 | 2018-06-28 | 10X Genomics Inc | Methods and compositions for nucleic acid analysis |
| US10626383B2 (en) | 2016-01-15 | 2020-04-21 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN110214186B (zh) | 2017-01-30 | 2023-11-24 | 10X基因组学有限公司 | 用于基于微滴的单细胞条形编码的方法和系统 |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| CN109526228B (zh) | 2017-05-26 | 2022-11-25 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
| US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| JP7111706B2 (ja) | 2017-06-07 | 2022-08-02 | タカラバイオ株式会社 | オリゴヌクレオチドの保存方法 |
| US11618891B2 (en) | 2017-06-26 | 2023-04-04 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| EP3954782A1 (en) | 2017-11-15 | 2022-02-16 | 10X Genomics, Inc. | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| CN112262218B (zh) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| KR102529958B1 (ko) * | 2021-01-18 | 2023-05-08 | 가톨릭대학교 산학협력단 | 저 gc 함량 dna의 정량적 증폭을 위한 pcr 첨가제 조성물 |
| CN116004776A (zh) * | 2022-08-30 | 2023-04-25 | 上海伯杰医疗科技股份有限公司 | 一管式RT-qPCR全预混反应试剂、应用及RT-qPCR方法 |
| WO2025071292A1 (ko) * | 2023-09-26 | 2025-04-03 | 주식회사 씨젠 | 핫 스타트 핵산 중합효소의 성능을 평가하는 방법 |
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| JPS61502282A (ja) | 1984-06-01 | 1986-10-09 | ナショナル・バイオメディカル・リサ−チ・ファウンデ−ション | 酵素剤を用いた触媒作用による核酸交雑方法 |
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| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
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| US5646019A (en) * | 1989-10-24 | 1997-07-08 | Stratagene | Method for producing primed nucleic acid templates |
| US5605824A (en) * | 1989-10-24 | 1997-02-25 | Stratagene | Composition for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| WO1991006679A1 (en) | 1989-10-24 | 1991-05-16 | Stratagene | An improved method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| US5449603A (en) * | 1989-10-24 | 1995-09-12 | Stratagene | Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
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| JP4632788B2 (ja) * | 2002-09-20 | 2011-02-16 | ニュー・イングランド・バイオラブズ・インコーポレイティッド | 核酸のヘリカーゼ依存性増幅 |
| JP2004113108A (ja) * | 2002-09-26 | 2004-04-15 | Aisin Seiki Co Ltd | Ｄｎａ回収法 |
| JP2005110621A (ja) * | 2003-10-10 | 2005-04-28 | Aisin Seiki Co Ltd | 核酸増幅方法及び核酸増幅用試薬キット |
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| US7700281B2 (en) * | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
-
2005
- 2005-06-29 US US11/171,008 patent/US7700281B2/en active Active
- 2005-06-30 CA CA002569394A patent/CA2569394A1/en not_active Abandoned
- 2005-06-30 EP EP11163362A patent/EP2366802A1/en not_active Withdrawn
- 2005-06-30 WO PCT/US2005/023824 patent/WO2006005074A2/en not_active Ceased
- 2005-06-30 EP EP05764400A patent/EP1778866B1/en not_active Expired - Lifetime
- 2005-06-30 AT AT05764400T patent/ATE527380T1/de not_active IP Right Cessation
- 2005-06-30 JP JP2007519541A patent/JP5020074B2/ja not_active Expired - Fee Related
-
2006
- 2006-11-28 NO NO20065470A patent/NO20065470L/no not_active Application Discontinuation
-
2007
- 2007-11-14 US US11/939,820 patent/US7951534B2/en not_active Expired - Lifetime
-
2011
- 2011-03-29 US US13/074,752 patent/US8404443B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP2008516584A (ja) | 2008-05-22 |
| NO20065470L (no) | 2007-01-26 |
| ATE527380T1 (de) | 2011-10-15 |
| EP2366802A1 (en) | 2011-09-21 |
| EP1778866B1 (en) | 2011-10-05 |
| WO2006005074A2 (en) | 2006-01-12 |
| US20060029952A1 (en) | 2006-02-09 |
| US7700281B2 (en) | 2010-04-20 |
| EP1778866A4 (en) | 2009-06-03 |
| EP1778866A2 (en) | 2007-05-02 |
| US8404443B2 (en) | 2013-03-26 |
| US20110189736A1 (en) | 2011-08-04 |
| JP5020074B2 (ja) | 2012-09-05 |
| US20080138878A1 (en) | 2008-06-12 |
| WO2006005074A3 (en) | 2008-06-19 |
| US7951534B2 (en) | 2011-05-31 |
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| Date | Code | Title | Description |
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| FZDE | Discontinued |