ATE527380T1 - Neue hot-start-nukleinsäureamplifikation - Google Patents
Neue hot-start-nukleinsäureamplifikationInfo
- Publication number
- ATE527380T1 ATE527380T1 AT05764400T AT05764400T ATE527380T1 AT E527380 T1 ATE527380 T1 AT E527380T1 AT 05764400 T AT05764400 T AT 05764400T AT 05764400 T AT05764400 T AT 05764400T AT E527380 T1 ATE527380 T1 AT E527380T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- reaction mixture
- acid amplification
- hot start
- new hot
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US58436204P | 2004-06-30 | 2004-06-30 | |
| PCT/US2005/023824 WO2006005074A2 (en) | 2004-06-30 | 2005-06-30 | Novel hot start nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE527380T1 true ATE527380T1 (de) | 2011-10-15 |
Family
ID=35783411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05764400T ATE527380T1 (de) | 2004-06-30 | 2005-06-30 | Neue hot-start-nukleinsäureamplifikation |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US7700281B2 (https=) |
| EP (2) | EP2366802A1 (https=) |
| JP (1) | JP5020074B2 (https=) |
| AT (1) | ATE527380T1 (https=) |
| CA (1) | CA2569394A1 (https=) |
| NO (1) | NO20065470L (https=) |
| WO (1) | WO2006005074A2 (https=) |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7700281B2 (en) * | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
| ATE502122T1 (de) | 2006-06-01 | 2011-04-15 | Trilink Biotechnologies | Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation |
| WO2009029648A2 (en) | 2007-08-27 | 2009-03-05 | Applera Corporation | Methods and compositions for pcr |
| EP2391732B1 (en) | 2009-01-30 | 2015-05-27 | Oxford Nanopore Technologies Limited | Methods using adaptors for nucleic acid constructs in transmembrane sequencing |
| JP5687414B2 (ja) | 2009-04-20 | 2015-03-18 | オリンパス株式会社 | 多型の識別方法 |
| KR101162422B1 (ko) * | 2009-11-30 | 2012-07-10 | 박수민 | 핵산 증폭용 조성물 |
| EP2856974B1 (en) | 2011-04-06 | 2017-03-01 | DePuy Synthes Products, LLC | Distal reamer |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| EP2859111B1 (en) * | 2012-06-08 | 2020-07-08 | Ionian Technologies, LLC | Nucleic acid amplifications |
| JP6450308B2 (ja) | 2012-06-14 | 2019-01-09 | ライフ テクノロジーズ コーポレーション | ポリメラーゼ連鎖反応(pcr)のための新規の組成物、方法、およびキット |
| US11155860B2 (en) * | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR20200140929A (ko) | 2013-02-08 | 2020-12-16 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| BR112016023625A2 (pt) | 2014-04-10 | 2018-06-26 | 10X Genomics, Inc. | dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| BR112017008877A2 (pt) | 2014-10-29 | 2018-07-03 | 10X Genomics Inc | métodos e composições para sequenciamento de ácido nucleico-alvo |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| JP6769969B2 (ja) | 2015-01-12 | 2020-10-14 | 10エックス ジェノミクス, インコーポレイテッド | 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー |
| MX2017010857A (es) | 2015-02-24 | 2017-12-11 | 10X Genomics Inc | Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana. |
| EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| JP6954899B2 (ja) | 2015-12-04 | 2021-10-27 | 10エックス ゲノミクス,インコーポレイテッド | 核酸の解析のための方法及び組成物 |
| WO2017121836A1 (en) | 2016-01-15 | 2017-07-20 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| EP3445876B1 (en) | 2017-05-26 | 2023-07-05 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| KR102724054B1 (ko) | 2017-06-07 | 2024-10-29 | 다카라 바이오 가부시키가이샤 | 올리고뉴클레오타이드의 보존 방법 |
| EP3645710A1 (en) | 2017-06-26 | 2020-05-06 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| CN112262218B (zh) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| KR102529958B1 (ko) * | 2021-01-18 | 2023-05-08 | 가톨릭대학교 산학협력단 | 저 gc 함량 dna의 정량적 증폭을 위한 pcr 첨가제 조성물 |
| CN116004776A (zh) * | 2022-08-30 | 2023-04-25 | 上海伯杰医疗科技股份有限公司 | 一管式RT-qPCR全预混反应试剂、应用及RT-qPCR方法 |
| WO2025071292A1 (ko) * | 2023-09-26 | 2025-04-03 | 주식회사 씨젠 | 핫 스타트 핵산 중합효소의 성능을 평가하는 방법 |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1985005685A1 (en) | 1984-06-01 | 1985-12-19 | National Biomedical Research Foundation | Catalyzed nucleic acid hybridization using enzymatic reagent |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US4994372A (en) | 1987-01-14 | 1991-02-19 | President And Fellows Of Harvard College | DNA sequencing |
| US5449603A (en) * | 1989-10-24 | 1995-09-12 | Stratagene | Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| US5646019A (en) * | 1989-10-24 | 1997-07-08 | Stratagene | Method for producing primed nucleic acid templates |
| US5605824A (en) * | 1989-10-24 | 1997-02-25 | Stratagene | Composition for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| WO1991006679A1 (en) | 1989-10-24 | 1991-05-16 | Stratagene | An improved method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| DK0515506T3 (da) | 1990-02-16 | 2000-05-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af carcinogene humane papillomavirus |
| US5223414A (en) * | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| CA2074214C (en) | 1991-07-23 | 2000-02-22 | Will Bloch | Improvements in the in situ pcr |
| WO1993015226A1 (en) * | 1992-01-28 | 1993-08-05 | President And Fellows Of Harvard College | Method for nucleic acid hybridization using single-stranded dna binding protein |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5364790A (en) * | 1993-02-16 | 1994-11-15 | The Perkin-Elmer Corporation | In situ PCR amplification system |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| EP0869187A3 (en) | 1997-02-24 | 2001-02-28 | Becton, Dickinson and Company | Replication of nucleic acids using single-strand DNA binding proteins |
| US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
| US6183998B1 (en) | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
| GB9920194D0 (en) | 1999-08-27 | 1999-10-27 | Advanced Biotech Ltd | A heat-stable thermostable DNA polymerase for use in nucleic acid amplification |
| WO2003008624A2 (en) | 2001-07-15 | 2003-01-30 | Keck Graduate Institute | Nucleic acid amplification using nicking agents |
| US7399590B2 (en) * | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| EP1539209A2 (en) * | 2002-09-05 | 2005-06-15 | Invitrogen Corporation | Compositions and methods for synthesizing nucleic acids |
| CA2498764C (en) | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
| JP2004113108A (ja) * | 2002-09-26 | 2004-04-15 | Aisin Seiki Co Ltd | Dna回収法 |
| JP2005110621A (ja) | 2003-10-10 | 2005-04-28 | Aisin Seiki Co Ltd | 核酸増幅方法及び核酸増幅用試薬キット |
| US7759057B2 (en) * | 2004-02-04 | 2010-07-20 | Ut-Battelle, Llc | Method for analyzing microbial communities |
| US7700281B2 (en) * | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
-
2005
- 2005-06-29 US US11/171,008 patent/US7700281B2/en active Active
- 2005-06-30 JP JP2007519541A patent/JP5020074B2/ja not_active Expired - Fee Related
- 2005-06-30 EP EP11163362A patent/EP2366802A1/en not_active Withdrawn
- 2005-06-30 AT AT05764400T patent/ATE527380T1/de not_active IP Right Cessation
- 2005-06-30 EP EP05764400A patent/EP1778866B1/en not_active Expired - Lifetime
- 2005-06-30 WO PCT/US2005/023824 patent/WO2006005074A2/en not_active Ceased
- 2005-06-30 CA CA002569394A patent/CA2569394A1/en not_active Abandoned
-
2006
- 2006-11-28 NO NO20065470A patent/NO20065470L/no not_active Application Discontinuation
-
2007
- 2007-11-14 US US11/939,820 patent/US7951534B2/en not_active Expired - Lifetime
-
2011
- 2011-03-29 US US13/074,752 patent/US8404443B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2006005074A3 (en) | 2008-06-19 |
| JP2008516584A (ja) | 2008-05-22 |
| CA2569394A1 (en) | 2006-01-12 |
| WO2006005074A2 (en) | 2006-01-12 |
| EP1778866B1 (en) | 2011-10-05 |
| US8404443B2 (en) | 2013-03-26 |
| EP1778866A4 (en) | 2009-06-03 |
| US20080138878A1 (en) | 2008-06-12 |
| EP1778866A2 (en) | 2007-05-02 |
| US20060029952A1 (en) | 2006-02-09 |
| NO20065470L (no) | 2007-01-26 |
| US7700281B2 (en) | 2010-04-20 |
| US7951534B2 (en) | 2011-05-31 |
| JP5020074B2 (ja) | 2012-09-05 |
| EP2366802A1 (en) | 2011-09-21 |
| US20110189736A1 (en) | 2011-08-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |