NO20065470L - Ny varmstart nukleinsyreamplifikasjon. - Google Patents

Ny varmstart nukleinsyreamplifikasjon.

Info

Publication number
NO20065470L
NO20065470L NO20065470A NO20065470A NO20065470L NO 20065470 L NO20065470 L NO 20065470L NO 20065470 A NO20065470 A NO 20065470A NO 20065470 A NO20065470 A NO 20065470A NO 20065470 L NO20065470 L NO 20065470L
Authority
NO
Norway
Prior art keywords
nucleic acid
reaction mixture
acid amplification
hot start
new hot
Prior art date
Application number
NO20065470A
Other languages
English (en)
Norwegian (no)
Inventor
Robert B Moffett
Jeannine Muller-Greven
Christopher James Kubu
Original Assignee
Usb Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Usb Corp filed Critical Usb Corp
Publication of NO20065470L publication Critical patent/NO20065470L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
NO20065470A 2004-06-30 2006-11-28 Ny varmstart nukleinsyreamplifikasjon. NO20065470L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58436204P 2004-06-30 2004-06-30
PCT/US2005/023824 WO2006005074A2 (en) 2004-06-30 2005-06-30 Novel hot start nucleic acid amplification

Publications (1)

Publication Number Publication Date
NO20065470L true NO20065470L (no) 2007-01-26

Family

ID=35783411

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20065470A NO20065470L (no) 2004-06-30 2006-11-28 Ny varmstart nukleinsyreamplifikasjon.

Country Status (7)

Country Link
US (3) US7700281B2 (https=)
EP (2) EP2366802A1 (https=)
JP (1) JP5020074B2 (https=)
AT (1) ATE527380T1 (https=)
CA (1) CA2569394A1 (https=)
NO (1) NO20065470L (https=)
WO (1) WO2006005074A2 (https=)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
AU2007268075C1 (en) 2006-06-01 2013-03-07 Trilink Biotechnologies Chemically modified oligonucleotide primers for nucleic acid amplification
JP2010537637A (ja) 2007-08-27 2010-12-09 アプライド バイオシステムズ インコーポレイテッド Ｐｃｒのための方法および組成物
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
JP5687414B2 (ja) * 2009-04-20 2015-03-18 オリンパス株式会社 多型の識別方法
KR101162422B1 (ko) * 2009-11-30 2012-07-10 박수민 핵산 증폭용 조성물
US9597188B2 (en) 2011-04-06 2017-03-21 DePuy Synthes Products, Inc. Version-replicating instrument and orthopaedic surgical procedure for using the same to implant a revision hip prosthesis
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
CN114214394A (zh) * 2012-06-08 2022-03-22 爱奥尼安技术公司 核苷酸扩增反应
KR20210072137A (ko) 2012-06-14 2021-06-16 라이프 테크놀로지스 코포레이션 폴리머라제 연쇄 반응 (pcr)을 위한 신규 조성물, 방법 및 키트
US11155860B2 (en) * 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA3216609C (en) 2012-08-14 2024-05-14 10X Genomics, Inc. Microcapsule compositions and methods
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016904A (es) 2014-06-26 2017-03-27 10X Genomics Inc Analisis de secuencias de acidos nucleicos.
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
MX2017005267A (es) 2014-10-29 2017-07-26 10X Genomics Inc Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana.
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
BR112017018054A2 (pt) 2015-02-24 2018-07-24 10X Genomics Inc métodos para a cobertura de sequências de ácidos nucleicos direcionadas
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
US10626383B2 (en) 2016-01-15 2020-04-21 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN110214186B (zh) 2017-01-30 2023-11-24 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
JP7111706B2 (ja) 2017-06-07 2022-08-02 タカラバイオ株式会社 オリゴヌクレオチドの保存方法
US11618891B2 (en) 2017-06-26 2023-04-04 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
KR102529958B1 (ko) * 2021-01-18 2023-05-08 가톨릭대학교 산학협력단 저 gc 함량 dna의 정량적 증폭을 위한 pcr 첨가제 조성물
CN116004776A (zh) * 2022-08-30 2023-04-25 上海伯杰医疗科技股份有限公司 一管式RT-qPCR全预混反应试剂、应用及RT-qPCR方法
WO2025071292A1 (ko) * 2023-09-26 2025-04-03 주식회사 씨젠 핫 스타트 핵산 중합효소의 성능을 평가하는 방법

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61502282A (ja) 1984-06-01 1986-10-09 ナショナル・バイオメディカル・リサ−チ・ファウンデ−ション 酵素剤を用いた触媒作用による核酸交雑方法
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4994372A (en) 1987-01-14 1991-02-19 President And Fellows Of Harvard College DNA sequencing
US5646019A (en) * 1989-10-24 1997-07-08 Stratagene Method for producing primed nucleic acid templates
US5605824A (en) * 1989-10-24 1997-02-25 Stratagene Composition for hybridizing nucleic acids using single-stranded nucleic acid binding protein
WO1991006679A1 (en) 1989-10-24 1991-05-16 Stratagene An improved method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
US5449603A (en) * 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
DE69131891T2 (de) * 1990-02-16 2000-06-15 F. Hoffmann-La Roche Ag, Basel Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
US5223414A (en) * 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
AU645915B2 (en) * 1991-07-23 1994-01-27 F. Hoffmann-La Roche Ag Improvements in the in situ PCR
WO1993015226A1 (en) * 1992-01-28 1993-08-05 President And Fellows Of Harvard College Method for nucleic acid hybridization using single-stranded dna binding protein
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5364790A (en) * 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
EP0869187A3 (en) 1997-02-24 2001-02-28 Becton, Dickinson and Company Replication of nucleic acids using single-strand DNA binding proteins
US6124120A (en) * 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6183998B1 (en) * 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
GB9920194D0 (en) * 1999-08-27 1999-10-27 Advanced Biotech Ltd A heat-stable thermostable DNA polymerase for use in nucleic acid amplification
WO2003008624A2 (en) * 2001-07-15 2003-01-30 Keck Graduate Institute Nucleic acid amplification using nicking agents
US7399590B2 (en) * 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
WO2004022770A2 (en) 2002-09-05 2004-03-18 Invitrogen Corporation Compositions and methods for synthesizing nucleic acids
JP4632788B2 (ja) * 2002-09-20 2011-02-16 ニュー・イングランド・バイオラブズ・インコーポレイティッド 核酸のヘリカーゼ依存性増幅
JP2004113108A (ja) * 2002-09-26 2004-04-15 Aisin Seiki Co Ltd Ｄｎａ回収法
JP2005110621A (ja) * 2003-10-10 2005-04-28 Aisin Seiki Co Ltd 核酸増幅方法及び核酸増幅用試薬キット
US7759057B2 (en) * 2004-02-04 2010-07-20 Ut-Battelle, Llc Method for analyzing microbial communities
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification

Also Published As

Publication number Publication date
JP2008516584A (ja) 2008-05-22
ATE527380T1 (de) 2011-10-15
EP2366802A1 (en) 2011-09-21
EP1778866B1 (en) 2011-10-05
CA2569394A1 (en) 2006-01-12
WO2006005074A2 (en) 2006-01-12
US20060029952A1 (en) 2006-02-09
US7700281B2 (en) 2010-04-20
EP1778866A4 (en) 2009-06-03
EP1778866A2 (en) 2007-05-02
US8404443B2 (en) 2013-03-26
US20110189736A1 (en) 2011-08-04
JP5020074B2 (ja) 2012-09-05
US20080138878A1 (en) 2008-06-12
WO2006005074A3 (en) 2008-06-19
US7951534B2 (en) 2011-05-31

Similar Documents

Publication Publication Date Title
NO20065470L (no) Ny varmstart nukleinsyreamplifikasjon.
CN102838654B (zh) 合成双功能复合物的方法
WO2005084367A3 (en) Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
NO20030557D0 (no) Fremgangsmåter og preparater til amplifisering av RNA- sekvenser
Tisné et al. The annealing mechanism of HIV-1 reverse transcription primer onto the viral genome
DK1948822T3 (da) Multikomponent-nukleinsyreenzymer og fremgangsmåder til deres anvendelse
DK1252328T3 (da) Fremgangsmåde og midler til isolering af mikroorganismer
CR9969A (es) Anticuerpos anti-globulomero ab, porciones de union a antigeno de estos, hibridomas correspondientes, acidos nucleicos, vectores, celulas huesped, metodos para producir dichos anticuerpos, composiciones que comprenden dichos anticuerpos
WO2007021944A3 (en) In vitro recombination method
Takahashi et al. Translation enhancer improves the ribosome liberation from translation initiation
ATE420170T1 (de) Matrizengerichtete split-and-mix-synthese von bibliotheken kleiner moleküle
WO2006127423A3 (en) Methods of producing polynucleotide libraries using scarless ligation
DK1411122T3 (da) Fremgangsmåde til fremstilling af nukleinsyremolekyler
NO20014641L (no) Spaltning av nukleinsyre fra faste b¶rere
SE0301951D0 (sv) New method
WO2005026730A3 (en) Multiplex binding and activity assays
WO2005019470A3 (en) Detecting and profiling molecular complexes
Reiter et al. Emerging structural themes in large RNA molecules
DE602004018270D1 (de) Nukleinsäureamplifikation mit nichtstandardbasen
JP2022088455A (ja) 熱安定性ポリメラーゼ阻害剤組成物及び方法
WO2006121888A3 (en) Methods of nucleic acid target capture
WO2002086155A3 (en) Method for the preferential nucleic acid amplification of selected nucleic acid regions
WO2005123956A3 (en) Rapid production of oligonucleotides
WO2006074334A3 (en) Identification of rna targets using helicases
ZA200501454B (en) Isolation and cloning of dna from uncultivated organism

Legal Events

Date Code Title Description
FC2A Withdrawal, rejection or dismissal of laid open patent application