NO20014641L - Spaltning av nukleinsyre fra faste b¶rere - Google Patents

Spaltning av nukleinsyre fra faste b¶rere

Info

Publication number
NO20014641L
NO20014641L NO20014641A NO20014641A NO20014641L NO 20014641 L NO20014641 L NO 20014641L NO 20014641 A NO20014641 A NO 20014641A NO 20014641 A NO20014641 A NO 20014641A NO 20014641 L NO20014641 L NO 20014641L
Authority
NO
Norway
Prior art keywords
nucleic acid
nucleotide
acid molecule
molecule
cleavage
Prior art date
Application number
NO20014641A
Other languages
English (en)
Other versions
NO20014641D0 (no
Inventor
Andrew Goldsborough
Original Assignee
Andrew Goldsborough
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Andrew Goldsborough filed Critical Andrew Goldsborough
Publication of NO20014641D0 publication Critical patent/NO20014641D0/no
Publication of NO20014641L publication Critical patent/NO20014641L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Det beskrives en fremgangsmåte for frigjøring av et nukleinsyremolekyl fra en fast bærer som det er festet til, hvor et ukonvensjonelt nukleotid er inkorporert i et forutbestemt sete i nevnte nukleinsyremolekyl, hvor fremgangsmåten omfatter selektiv avspaltning av nevnte nukleinsyremolekyl fra setet for nevnte ukonvensjonelle nukleotid. Nukleinsyremolekylet kan være et kimært molekyl som omfatter en nukleinsyre (nukleotidsekvens) og en annen molekylkomponent med en forskjellig kjemisk natur. Derved tilveiebringes også et kimært molekyl (eller konstruksjon) som omfatter en nukleotid-linkersekvens omfattende et selektivt avspaltbart ukonvensjonelt nukleotid fra et forutbestemt sete, koblet til en funksjonell gruppe, som kan være en affinitetsbindingsgruppe eller en rapportørgruppe. Anvendelsesområder kan for eksempel være molekylærbiologiske prosedyrer, så som nukleinsyresyntese, amplifikasjon eller sekvensering, nukleinsyreisolering eller affinitets-baserte separasjons- eller testprosedyrer.
NO20014641A 1999-03-29 2001-09-25 Spaltning av nukleinsyre fra faste b¶rere NO20014641L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9907245.6A GB9907245D0 (en) 1999-03-29 1999-03-29 Cleavage of nucleic acids from solid supports
PCT/GB2000/001190 WO2000058329A1 (en) 1999-03-29 2000-03-28 Cleavage of nucleic acid from solid supports

Publications (2)

Publication Number Publication Date
NO20014641D0 NO20014641D0 (no) 2001-09-25
NO20014641L true NO20014641L (no) 2001-11-27

Family

ID=10850600

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20014641A NO20014641L (no) 1999-03-29 2001-09-25 Spaltning av nukleinsyre fra faste b¶rere

Country Status (13)

Country Link
EP (1) EP1165585A1 (no)
JP (1) JP2003500013A (no)
KR (1) KR20020013517A (no)
CN (1) CN1345328A (no)
AU (1) AU3566800A (no)
BR (1) BR0009395A (no)
CA (1) CA2368420A1 (no)
GB (1) GB9907245D0 (no)
IL (1) IL145411A0 (no)
MX (1) MXPA01009698A (no)
NO (1) NO20014641L (no)
NZ (1) NZ514852A (no)
WO (1) WO2000058329A1 (no)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
JP4518754B2 (ja) * 2003-06-30 2010-08-04 パナソニック株式会社 ヌクレオチド鎖修飾方法
WO2005014808A1 (ja) * 2003-06-30 2005-02-17 Matsushita Electric Industrial Co., Ltd. ヌクレオチド鎖修飾方法
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
KR101881596B1 (ko) 2008-12-02 2018-07-24 웨이브 라이프 사이언시스 재팬 인코포레이티드 인 원자 변형된 핵산의 합성 방법
SG10201403841QA (en) 2009-07-06 2014-09-26 Ontorii Inc Novel nucleic acid prodrugs and methods of use thereof
JP5847700B2 (ja) 2010-03-05 2016-01-27 株式会社Ｗａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｊａｐａｎ リボヌクレオシドホスホロチオエートの製造方法
EP2620428B1 (en) 2010-09-24 2019-05-22 Wave Life Sciences Ltd. Asymmetric auxiliary group
JP6128529B2 (ja) 2011-07-19 2017-05-17 ウェイブ ライフ サイエンシズ リミテッドＷａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｌｔｄ． 官能化核酸の合成のための方法
US9598458B2 (en) 2012-07-13 2017-03-21 Wave Life Sciences Japan, Inc. Asymmetric auxiliary group
EP4219516A3 (en) 2012-07-13 2024-01-10 Wave Life Sciences Ltd. Chiral control
BR112015000723A2 (pt) 2012-07-13 2017-06-27 Shin Nippon Biomedical Laboratories Ltd adjuvante de ácido nucléico quiral
EP3095461A4 (en) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having immunity induction activity, and immunity induction activator
WO2015108048A1 (ja) 2014-01-15 2015-07-23 株式会社新日本科学 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤
WO2015108046A1 (ja) 2014-01-15 2015-07-23 株式会社新日本科学 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤
BR112016016400A2 (pt) 2014-01-16 2017-10-03 Wave Life Sciences Ltd Composições de oligonucleotídeos quiralmente controlados, seu uso, sua composição farmacêutica, e métodos
US20170022546A1 (en) * 2014-03-10 2017-01-26 Rashid Bashir Detection and quantification of methylation in dna
GB201717446D0 (en) 2017-10-24 2017-12-06 Evox Therapeutics Ltd Affinity purification of engineering extracellular vesicles
CN109956987B (zh) * 2017-12-14 2022-05-17 南京金斯瑞生物科技有限公司 氨解方法及氨解组合物

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5367066A (en) * 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
SE9003743D0 (sv) * 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers

Also Published As

Publication number Publication date
EP1165585A1 (en) 2002-01-02
JP2003500013A (ja) 2003-01-07
AU3566800A (en) 2000-10-16
KR20020013517A (ko) 2002-02-20
IL145411A0 (en) 2002-06-30
CA2368420A1 (en) 2000-10-05
GB9907245D0 (en) 1999-05-26
MXPA01009698A (es) 2003-06-24
NZ514852A (en) 2003-07-25
BR0009395A (pt) 2001-12-26
CN1345328A (zh) 2002-04-17
NO20014641D0 (no) 2001-09-25
WO2000058329A1 (en) 2000-10-05

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