MXPA01009698A - Desdoblamiento de acidos nucleicos de soportes solidos. - Google Patents

Desdoblamiento de acidos nucleicos de soportes solidos.

Info

Publication number
MXPA01009698A
MXPA01009698A MXPA01009698A MXPA01009698A MXPA01009698A MX PA01009698 A MXPA01009698 A MX PA01009698A MX PA01009698 A MXPA01009698 A MX PA01009698A MX PA01009698 A MXPA01009698 A MX PA01009698A MX PA01009698 A MXPA01009698 A MX PA01009698A
Authority
MX
Mexico
Prior art keywords
nucleic acid
nucleotide
acid molecule
molecule
site
Prior art date
Application number
MXPA01009698A
Other languages
English (en)
Inventor
Andrew Goldsborough
Original Assignee
Andrew Goldsborough
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Andrew Goldsborough filed Critical Andrew Goldsborough
Publication of MXPA01009698A publication Critical patent/MXPA01009698A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente invencion proporciona un metodo para separar una molecula de acido nucleico de un soporte solido al cual esta enlazada, en donde se incorpora un nucleotido no convencional en un sitio predeterminado en la molecula de acido nucleico, el metodo comprende desdoblar selectivamente la molecula de acido nucleico en el sitio del nucleotido no convencional. La molecula de acido nucleico puede ser una molecula quimerica que comprende un acido nucleico (secuencia de nucleotidos) y otro componente molecular de una naturaleza quimica diferente. Este aspecto de la invencion tambien proporciona una molecula quimerica (o constructo) que comprende una secuencia de enlace nucleotido que comprende un nucleotido no convencional selectivamente desdoblado en un sitio predeterminado, acoplado a un grupo funcional, que puede ser un grupo de enlace de afinidad o un grupo reportero. La invencion encuentra utilidad en un numero de aplicaciones, por ejemplo, procedimientos biologicos moleculares tales como sintesis de acido nucleico, amplificacion o formacion de secuencias, aislamiento de acido nucleico o en separacion basada en afinidad o procedimientos de ensayo.
MXPA01009698A 1999-03-29 2000-03-28 Desdoblamiento de acidos nucleicos de soportes solidos. MXPA01009698A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9907245.6A GB9907245D0 (en) 1999-03-29 1999-03-29 Cleavage of nucleic acids from solid supports
PCT/GB2000/001190 WO2000058329A1 (en) 1999-03-29 2000-03-28 Cleavage of nucleic acid from solid supports

Publications (1)

Publication Number Publication Date
MXPA01009698A true MXPA01009698A (es) 2003-06-24

Family

ID=10850600

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA01009698A MXPA01009698A (es) 1999-03-29 2000-03-28 Desdoblamiento de acidos nucleicos de soportes solidos.

Country Status (13)

Country Link
EP (1) EP1165585A1 (es)
JP (1) JP2003500013A (es)
KR (1) KR20020013517A (es)
CN (1) CN1345328A (es)
AU (1) AU3566800A (es)
BR (1) BR0009395A (es)
CA (1) CA2368420A1 (es)
GB (1) GB9907245D0 (es)
IL (1) IL145411A0 (es)
MX (1) MXPA01009698A (es)
NO (1) NO20014641L (es)
NZ (1) NZ514852A (es)
WO (1) WO2000058329A1 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
JP4518754B2 (ja) * 2003-06-30 2010-08-04 パナソニック株式会社 ヌクレオチド鎖修飾方法
KR100683025B1 (ko) * 2003-06-30 2007-02-15 마츠시타 덴끼 산교 가부시키가이샤 뉴클레오티드쇄 수식방법
GB0514936D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
CA2744987C (en) 2008-12-02 2018-01-16 Chiralgen, Ltd. Method for the synthesis of phosphorus atom modified nucleic acids
AU2010270714B2 (en) 2009-07-06 2015-08-13 Wave Life Sciences Ltd. Novel nucleic acid prodrugs and methods use thereof
WO2011108682A1 (ja) * 2010-03-05 2011-09-09 国立大学法人 東京大学 リボヌクレオシドホスホロチオエートの製造方法
EP2620428B1 (en) 2010-09-24 2019-05-22 Wave Life Sciences Ltd. Asymmetric auxiliary group
SG10201700554VA (en) 2011-07-19 2017-03-30 Wave Life Sciences Pte Ltd Methods for the synthesis of functionalized nucleic acids
WO2014012081A2 (en) 2012-07-13 2014-01-16 Ontorii, Inc. Chiral control
SG11201500239VA (en) 2012-07-13 2015-03-30 Wave Life Sciences Japan Asymmetric auxiliary group
BR112015000723A2 (pt) 2012-07-13 2017-06-27 Shin Nippon Biomedical Laboratories Ltd adjuvante de ácido nucléico quiral
US10149905B2 (en) 2014-01-15 2018-12-11 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having antitumor effect and antitumor agent
EP3095461A4 (en) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having immunity induction activity, and immunity induction activator
JPWO2015108046A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤
MX2016009290A (es) 2014-01-16 2017-02-28 Wave Life Sciences Ltd Diseño quiral.
US20170022546A1 (en) * 2014-03-10 2017-01-26 Rashid Bashir Detection and quantification of methylation in dna
GB201717446D0 (en) 2017-10-24 2017-12-06 Evox Therapeutics Ltd Affinity purification of engineering extracellular vesicles
CN109956987B (zh) * 2017-12-14 2022-05-17 南京金斯瑞生物科技有限公司 氨解方法及氨解组合物

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5367066A (en) * 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
SE9003743D0 (sv) * 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers

Also Published As

Publication number Publication date
JP2003500013A (ja) 2003-01-07
NO20014641D0 (no) 2001-09-25
WO2000058329A1 (en) 2000-10-05
CA2368420A1 (en) 2000-10-05
NZ514852A (en) 2003-07-25
NO20014641L (no) 2001-11-27
IL145411A0 (en) 2002-06-30
GB9907245D0 (en) 1999-05-26
KR20020013517A (ko) 2002-02-20
EP1165585A1 (en) 2002-01-02
CN1345328A (zh) 2002-04-17
BR0009395A (pt) 2001-12-26
AU3566800A (en) 2000-10-16

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