ATE495436T1 - Verfahren zur isolierung und markierung von probenmolekülen - Google Patents

Verfahren zur isolierung und markierung von probenmolekülen

Info

Publication number
ATE495436T1
ATE495436T1 AT02731818T AT02731818T ATE495436T1 AT E495436 T1 ATE495436 T1 AT E495436T1 AT 02731818 T AT02731818 T AT 02731818T AT 02731818 T AT02731818 T AT 02731818T AT E495436 T1 ATE495436 T1 AT E495436T1
Authority
AT
Austria
Prior art keywords
group
sample molecule
tag
functional group
labeling
Prior art date
Application number
AT02731818T
Other languages
English (en)
Inventor
Rudolf Aebersold
Huilin Zhou
Beate Rist
George Vella
Subhasish Purkayastha
Sasi Pillai
Original Assignee
Inst Systems Biology
Univ Washington
Dh Technologies Dev Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Systems Biology, Univ Washington, Dh Technologies Dev Pte Ltd filed Critical Inst Systems Biology
Application granted granted Critical
Publication of ATE495436T1 publication Critical patent/ATE495436T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Peptides Or Proteins (AREA)
AT02731818T 2001-05-14 2002-05-14 Verfahren zur isolierung und markierung von probenmolekülen ATE495436T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/858,198 US7183116B2 (en) 2001-05-14 2001-05-14 Methods for isolation and labeling of sample molecules
PCT/US2002/015500 WO2002093131A2 (en) 2001-05-14 2002-05-14 Methods for isolating and labeling sample molecules

Publications (1)

Publication Number Publication Date
ATE495436T1 true ATE495436T1 (de) 2011-01-15

Family

ID=25327723

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02731818T ATE495436T1 (de) 2001-05-14 2002-05-14 Verfahren zur isolierung und markierung von probenmolekülen

Country Status (9)

Country Link
US (3) US7183116B2 (de)
EP (1) EP1456632B1 (de)
JP (2) JP4414654B2 (de)
CN (1) CN1602422B (de)
AT (1) ATE495436T1 (de)
AU (1) AU2002303760B2 (de)
CA (1) CA2447874C (de)
DE (1) DE60238940D1 (de)
WO (1) WO2002093131A2 (de)

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EP0963435B1 (de) 1997-01-08 2008-05-28 Invitrogen Corporation Verfahren zur herstellung von proteinen
ATE344456T1 (de) * 2000-05-05 2006-11-15 Purdue Research Foundation Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen
US20030017507A1 (en) * 2001-05-30 2003-01-23 Johnson Richard S. Relative protein quantitation phosphoproteins using stable isotope labeling
EP2053406A3 (de) 2001-07-16 2009-06-24 caprotec bioanalytics GmbH Capture Compounds, Sammlung derselben und Verfahren zur Proteomanalyse und Komplexzusammensetzung
AU2002341862C1 (en) * 2001-09-27 2009-01-29 Purdue Research Foundation Controlling isotope effects during fractionation of analytes
JP4547153B2 (ja) * 2001-12-11 2010-09-22 ユニバーシティ オブ メディスン アンド デンティストリー オブ ニュー ジャージー タンパク質プロファイリングのための診断方法
CA2473376A1 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
WO2003078962A2 (en) * 2002-03-11 2003-09-25 President And Fellows Of Harvard College Detection and quantification of modified proteins
DE60325847D1 (de) * 2002-03-11 2009-03-05 Caprotec Bioanalytics Gmbh Verbindungen und verfahren für die analyse des proteoms
US20070269895A1 (en) * 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
WO2003102018A2 (en) * 2002-06-03 2003-12-11 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
JP2004170195A (ja) * 2002-11-19 2004-06-17 Reverse Proteomics Research Institute Co Ltd タンパク質の固定化方法
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
CA2658334C (en) * 2003-01-16 2012-07-10 Caprotec Bioanalytics Gmbh Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
WO2005007677A2 (en) * 2003-07-10 2005-01-27 The Institute For Systems Biology Affinity capture of peptides by microarray and related methods
US20050153306A1 (en) * 2003-07-14 2005-07-14 Irm Llc Fluorogenic enzyme substrates and uses thereof
US20050113584A1 (en) * 2003-07-14 2005-05-26 Irm Llc Methods for the preparation of rhodamine
WO2005015239A2 (en) * 2003-08-07 2005-02-17 Cornell Research Foundation, Inc. Method for n-terminal labeling of proteins
JP4363935B2 (ja) * 2003-09-17 2009-11-11 株式会社豊田中央研究所 生体物質の光固定化・回収方法及び表面プラズモン共鳴センサー
WO2005030981A2 (en) * 2003-09-25 2005-04-07 Invitrogen Corporation Homogeneous populations of molecules
CA2572754A1 (en) * 2004-07-12 2006-02-16 Applera Corporation Mass tags for quantitative analyses
US20070048752A1 (en) * 2004-07-12 2007-03-01 Applera Corporation Mass tags for quantitative analyses
WO2006011673A1 (ja) * 2004-07-30 2006-02-02 Reverse Proteomics Research Institute Co., Ltd. 光切断型リンカーを利用したリガンド固定化固相担体
JP4635532B2 (ja) * 2004-09-14 2011-02-23 株式会社ニコン 生体分子の分析および回収方法
US20060134694A1 (en) * 2004-12-22 2006-06-22 Intel Corporation Methods of protein profiling by thiolation
CA2599731A1 (en) * 2005-03-03 2006-09-08 Dmi Biosciences, Inc. Quantification of proteins
EP1891426B1 (de) * 2005-06-07 2011-10-12 Centre National De La Recherche Scientifique -Cnrs- Verwendung ionischer matrizen zur analyse von gewebeabschnitten durch maldi-massenspektrometrie
GB0515323D0 (en) * 2005-07-26 2005-08-31 Electrophoretics Ltd Mass labels
US7906341B2 (en) * 2006-06-30 2011-03-15 Dh Technologies Development Pte, Ltd. Methods, mixtures, kits and compositions pertaining to analyte determination
WO2008042495A2 (en) 2006-07-21 2008-04-10 Life Technologies Corporation Sharply resolving labeled protein molecular weight standards
EP2108111B1 (de) * 2006-12-07 2019-04-24 Definitek, Inc. Festphasen-katalysierte und -aktivierte automatisierte isotopenverdünnung und speziierte isotopenverdünnungs-massenspektrometrie
US7728287B2 (en) * 2007-03-01 2010-06-01 Lawrence Livermore National Security, Llc Imaging mass spectrometer with mass tags
CN102648312B (zh) * 2009-07-22 2014-03-05 新加坡科技研究局 同质量氨基酸和其它物质的区分
EP2609426B1 (de) * 2010-08-27 2018-12-19 The Texas A&M University System Fluoreszenzmarkierungsreagenzien und deren verwendungen
US8492163B2 (en) 2011-01-31 2013-07-23 Dh Technologies Development Pte. Ltd. Methods, mixtures, kits and compositions pertaining to analyte determination
GB2528643A (en) * 2014-07-08 2016-02-03 Adc Biotechnology Ltd Method of synthesising ADCs
JP2017020896A (ja) * 2015-07-10 2017-01-26 国立大学法人北陸先端科学技術大学院大学 Ｎ末端標識剤、これを用いた蛍光標識タンパク質及びその製造方法
CA2990895C (en) * 2015-07-29 2023-03-21 Nils A. KULAK Means and methods for a sample preparation, especially for mass spectrometry
CN110687297B (zh) * 2018-07-06 2023-08-22 复旦大学 基于稳定同位素半胱氨酸代谢标记的棕榈酰化修饰蛋白质定量分析方法
GB201913598D0 (en) * 2019-09-20 2019-11-06 Univ Birmingham Labelling of biomolecules
CN113075114B (zh) * 2019-12-17 2022-07-01 北京大学 一种用于单细胞分析的有机质谱流式分析方法

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US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5986076A (en) 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
DE69531545T2 (de) 1994-06-23 2004-06-24 Affymax Technologies N.V., Willemstad Photolabile verbindungen und verfahren für deren verwendung
NZ502088A (en) 1997-07-11 2002-05-31 Brax Group Ltd Characterising nucleic acids using mass spectroscopy
ATE253126T1 (de) * 1998-08-25 2003-11-15 Univ Washington Schnelle quantitative analyse von proteinen oder proteinfunktionen in komplexen gemischen
JP2004503780A (ja) 2000-06-12 2004-02-05 ユニバーシティ　オブ　ワシントン リンペプチドの選択的標識および単離ならびにプロテオーム分析への適用
AU4338502A (en) 2000-10-23 2002-06-24 Genetics Inst Acid-labile isotope-coded extractant (alice) and its use in quantitative mass spectrometric analysis of protein mixtures

Also Published As

Publication number Publication date
US20020168644A1 (en) 2002-11-14
US7183116B2 (en) 2007-02-27
DE60238940D1 (de) 2011-02-24
EP1456632B1 (de) 2011-01-12
JP2009175157A (ja) 2009-08-06
CN1602422A (zh) 2005-03-30
EP1456632A4 (de) 2005-04-20
JP2005503540A (ja) 2005-02-03
US20040110186A1 (en) 2004-06-10
CN1602422B (zh) 2011-06-08
US20040265810A1 (en) 2004-12-30
US7364911B2 (en) 2008-04-29
JP4414654B2 (ja) 2010-02-10
CA2447874A1 (en) 2002-11-21
WO2002093131A2 (en) 2002-11-21
WO2002093131A3 (en) 2004-05-27
EP1456632A2 (de) 2004-09-15
AU2002303760B2 (en) 2008-04-10
CA2447874C (en) 2010-08-31

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