ATE495436T1 - Verfahren zur isolierung und markierung von probenmolekülen - Google Patents

Verfahren zur isolierung und markierung von probenmolekülen

Info

Publication number
ATE495436T1
ATE495436T1 AT02731818T AT02731818T ATE495436T1 AT E495436 T1 ATE495436 T1 AT E495436T1 AT 02731818 T AT02731818 T AT 02731818T AT 02731818 T AT02731818 T AT 02731818T AT E495436 T1 ATE495436 T1 AT E495436T1
Authority
AT
Austria
Prior art keywords
group
sample molecule
tag
functional group
labeling
Prior art date
Application number
AT02731818T
Other languages
English (en)
Inventor
Rudolf Aebersold
Huilin Zhou
Beate Rist
George Vella
Subhasish Purkayastha
Sasi Pillai
Original Assignee
Inst Systems Biology
Univ Washington
Dh Technologies Dev Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Systems Biology, Univ Washington, Dh Technologies Dev Pte Ltd filed Critical Inst Systems Biology
Application granted granted Critical
Publication of ATE495436T1 publication Critical patent/ATE495436T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
AT02731818T 2001-05-14 2002-05-14 Verfahren zur isolierung und markierung von probenmolekülen ATE495436T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/858,198 US7183116B2 (en) 2001-05-14 2001-05-14 Methods for isolation and labeling of sample molecules
PCT/US2002/015500 WO2002093131A2 (en) 2001-05-14 2002-05-14 Methods for isolating and labeling sample molecules

Publications (1)

Publication Number Publication Date
ATE495436T1 true ATE495436T1 (de) 2011-01-15

Family

ID=25327723

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02731818T ATE495436T1 (de) 2001-05-14 2002-05-14 Verfahren zur isolierung und markierung von probenmolekülen

Country Status (9)

Country Link
US (3) US7183116B2 (de)
EP (1) EP1456632B1 (de)
JP (2) JP4414654B2 (de)
CN (1) CN1602422B (de)
AT (1) ATE495436T1 (de)
AU (1) AU2002303760B2 (de)
CA (1) CA2447874C (de)
DE (1) DE60238940D1 (de)
WO (1) WO2002093131A2 (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
JP2001512306A (ja) 1997-01-08 2001-08-21 ライフ テクノロジーズ，インコーポレイテッド タンパク質の産生方法
AU2001259470A1 (en) * 2000-05-05 2001-11-20 Purdue Research Foundation Affinity selected signature peptides for protein identification and quantification
US20030017507A1 (en) * 2001-05-30 2003-01-23 Johnson Richard S. Relative protein quantitation phosphoproteins using stable isotope labeling
DE60230927D1 (de) 2001-07-16 2009-03-05 Caprotec Bioanalytics Gmbh Fangverbindungen, ihre entnahme und verfahren zur analysierung des proteoms und von komplexen zusammensetzungen
AU2002341862C1 (en) * 2001-09-27 2009-01-29 Purdue Research Foundation Controlling isotope effects during fractionation of analytes
WO2003049602A2 (en) * 2001-12-11 2003-06-19 University Of Medicine And Dentistry Of New Jersey Diagnostic methods for protein profiling
JP2005538929A (ja) 2002-01-16 2005-12-22 ダイナル バイオテック エイエスエイ 単一サンプルからの核酸及びタンパク質の単離方法
ES2319634T3 (es) * 2002-03-11 2009-05-11 Caprotec Bioanalytics Gmbh Compuestos y metodos para analizar el proteoma.
WO2003078962A2 (en) * 2002-03-11 2003-09-25 President And Fellows Of Harvard College Detection and quantification of modified proteins
AU2003249692B2 (en) * 2002-06-03 2008-07-31 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
US20070269895A1 (en) * 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
JP2004170195A (ja) * 2002-11-19 2004-06-17 Reverse Proteomics Research Institute Co Ltd タンパク質の固定化方法
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
JP4741458B2 (ja) * 2003-01-16 2011-08-03 カプロテック・バイオアナリティクス・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング 捕獲化合物、その収集物ならびにプロテオームおよび複合組成物を分析するための方法
US7794947B2 (en) * 2003-07-10 2010-09-14 Institute For Systems Biology Affinity capture of peptides by microarray and related methods
US20050153306A1 (en) * 2003-07-14 2005-07-14 Irm Llc Fluorogenic enzyme substrates and uses thereof
WO2005007678A2 (en) * 2003-07-14 2005-01-27 Irm Llc Methods for the preparation of rhodamine
WO2005015239A2 (en) * 2003-08-07 2005-02-17 Cornell Research Foundation, Inc. Method for n-terminal labeling of proteins
JP4363935B2 (ja) * 2003-09-17 2009-11-11 株式会社豊田中央研究所 生体物質の光固定化・回収方法及び表面プラズモン共鳴センサー
WO2005030981A2 (en) * 2003-09-25 2005-04-07 Invitrogen Corporation Homogeneous populations of molecules
CA2572754A1 (en) * 2004-07-12 2006-02-16 Applera Corporation Mass tags for quantitative analyses
US20070048752A1 (en) * 2004-07-12 2007-03-01 Applera Corporation Mass tags for quantitative analyses
US7456022B2 (en) * 2004-07-30 2008-11-25 Reverse Proteomics Research Institute Co., Ltd. Solid support having ligand immobilized thereon by using photocleavable linker
JP4635532B2 (ja) * 2004-09-14 2011-02-23 株式会社ニコン 生体分子の分析および回収方法
US20060134694A1 (en) * 2004-12-22 2006-06-22 Intel Corporation Methods of protein profiling by thiolation
WO2006094185A2 (en) * 2005-03-03 2006-09-08 Dmi Biosciences Inc. Quantification of proteins
ATE520022T1 (de) * 2005-06-07 2011-08-15 Centre Nat Rech Scient Verwendung von konjugaten mit mittels photodissoziation oder fragmentation spaltbaren bindern für die massenspektrometrieanalyse von gewebeschnitten
GB0515323D0 (en) * 2005-07-26 2005-08-31 Electrophoretics Ltd Mass labels
US7906341B2 (en) * 2006-06-30 2011-03-15 Dh Technologies Development Pte, Ltd. Methods, mixtures, kits and compositions pertaining to analyte determination
EP2069386A4 (de) 2006-07-21 2009-10-28 Life Technologies Corp Proteinmolekülgewichtsstandards mit hoher trennschärfe
US8383420B2 (en) * 2006-12-07 2013-02-26 Applied Isotope Technologies, Inc. Solid phase and catalyzed enabled automated isotope dilution and speciated isotope dilution mass spectrometry
US7728287B2 (en) * 2007-03-01 2010-06-01 Lawrence Livermore National Security, Llc Imaging mass spectrometer with mass tags
US9863956B2 (en) 2009-07-22 2018-01-09 Agency For Science, Technology And Research Differentiation of isobaric amino acids and other species
WO2012027717A2 (en) 2010-08-27 2012-03-01 The Texas A&M University System Flourescence labeling reagents and uses thereof
US8492163B2 (en) 2011-01-31 2013-07-23 Dh Technologies Development Pte. Ltd. Methods, mixtures, kits and compositions pertaining to analyte determination
GB2528643A (en) * 2014-07-08 2016-02-03 Adc Biotechnology Ltd Method of synthesising ADCs
JP2017020896A (ja) * 2015-07-10 2017-01-26 国立大学法人北陸先端科学技術大学院大学 Ｎ末端標識剤、これを用いた蛍光標識タンパク質及びその製造方法
CA2990895C (en) * 2015-07-29 2023-03-21 Nils A. KULAK Means and methods for a sample preparation, especially for mass spectrometry
CN110687297B (zh) * 2018-07-06 2023-08-22 复旦大学 基于稳定同位素半胱氨酸代谢标记的棕榈酰化修饰蛋白质定量分析方法
GB201913598D0 (en) * 2019-09-20 2019-11-06 Univ Birmingham Labelling of biomolecules
CN113075114B (zh) * 2019-12-17 2022-07-01 北京大学 一种用于单细胞分析的有机质谱流式分析方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5986076A (en) 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
AU689924B2 (en) 1994-06-23 1998-04-09 Affymax Technologies N.V. Photolabile compounds and methods for their use
EP1002128A1 (de) 1997-07-11 2000-05-24 Brax Genomics Limited Charakterisierung von nukleinsäuren
DE69912444T3 (de) * 1998-08-25 2010-05-06 University Of Washington, Seattle Schnelle quantitative analyse von proteinen oder proteinfunktionen in komplexen gemischen
EP1295123A4 (de) 2000-06-12 2005-02-02 Univ Washington Selektives markieren und isolieren von phosphopeptiden und anwendungen in proteomischen analysen
CA2426731A1 (en) 2000-10-23 2002-06-20 Genetics Institute, Llc. Acid-labile isotope-coded extractant (alice) and its use in quantitative mass spectrometric analysis of protein mixtures

Also Published As

Publication number Publication date
US7183116B2 (en) 2007-02-27
US20020168644A1 (en) 2002-11-14
JP2005503540A (ja) 2005-02-03
WO2002093131A3 (en) 2004-05-27
AU2002303760B2 (en) 2008-04-10
CN1602422A (zh) 2005-03-30
US7364911B2 (en) 2008-04-29
US20040265810A1 (en) 2004-12-30
CA2447874C (en) 2010-08-31
EP1456632A4 (de) 2005-04-20
CN1602422B (zh) 2011-06-08
EP1456632A2 (de) 2004-09-15
DE60238940D1 (de) 2011-02-24
US20040110186A1 (en) 2004-06-10
EP1456632B1 (de) 2011-01-12
WO2002093131A2 (en) 2002-11-21
JP2009175157A (ja) 2009-08-06
JP4414654B2 (ja) 2010-02-10
CA2447874A1 (en) 2002-11-21

Similar Documents

Publication Publication Date Title
ATE495436T1 (de) Verfahren zur isolierung und markierung von probenmolekülen
DE60010058D1 (de) Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
WO2004086050A3 (en) Labelling agents for mass spectrometry comprising tertiary amines
DE69900462D1 (de) Konjugate von dns und pns und verfahren zu deren herstellung
CY1111278T1 (el) Συστημα ασφαλειας
DK2385141T3 (da) Flerkomponentnukleinsyreenzymer og fremgangsmåder til anvendelse heraf
ATE419629T1 (de) Poröse ferro- oder ferrimagnetische glasteilchen für molekültrennung
ATE191934T1 (de) Verfahren zur isothermer amplifikation von nukleinsäure molekülen
DE69937447D1 (de) Verfahren zur erkennung von nukleinsäuren
US20060099626A1 (en) DNA-templated combinatorial library device and method for use
ATE532066T1 (de) Oberflächenimmobilisierter polyelektrolyt mit mehreren, zur kovalenten bindung an biomoleküle fähigen funktionellen gruppen
ATE443071T1 (de) Verfahren zum entschützen von oligonukleotiden
NO20014641L (no) Spaltning av nukleinsyre fra faste b¶rere
DE60303020D1 (de) Verfahren zur gewinnung von nukleinsäuren mit hilfe chaotroper-und ammoniumreagentien
EP1983052A3 (de) Verfahren zum Klonen und zur Herstellung von Fragmentketten mit lesbarem Informationsinhalt
AU3143897A (en) Oligonucleotides which specifically bind retroviral nucleocapsid proteins
EA200701578A1 (ru) Способ конъюгирования молекул, содержащих аминотиол, к носителям
Sachdeva et al. Covalent tagging of phosphorylated peptides by phosphate-specific deoxyribozymes
WO2008094205A3 (en) A mild chemically cleavable linker system
ATE402198T1 (de) Verfahren zur immobilisierung biologischer moleküle an feste oberflächen
WO2003025567A3 (de) Herstellung von trägergebundenen molekülen mittels oligonucleotid-tags
EP1083980A4 (de) Modulation molekularer wechselwirkungspositionen in rns und anderen biomolekülen
SE9904222L (sv) Nya funktionaliserade polymera reagens
ATE514083T1 (de) Verfahren und zusammensetzungen zur reinheitsbestimmung und reinigung chemisch synthetisierter nukleinsäuren

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties