ATE495436T1 - Verfahren zur isolierung und markierung von probenmolekülen - Google Patents

Verfahren zur isolierung und markierung von probenmolekülen

Info

Publication number
ATE495436T1
ATE495436T1 AT02731818T AT02731818T ATE495436T1 AT E495436 T1 ATE495436 T1 AT E495436T1 AT 02731818 T AT02731818 T AT 02731818T AT 02731818 T AT02731818 T AT 02731818T AT E495436 T1 ATE495436 T1 AT E495436T1
Authority
AT
Austria
Prior art keywords
group
sample molecule
tag
functional group
labeling
Prior art date
Application number
AT02731818T
Other languages
English (en)
Inventor
Rudolf Aebersold
Huilin Zhou
Beate Rist
George Vella
Subhasish Purkayastha
Sasi Pillai
Original Assignee
Inst Systems Biology
Univ Washington
Dh Technologies Dev Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Systems Biology, Univ Washington, Dh Technologies Dev Pte Ltd filed Critical Inst Systems Biology
Application granted granted Critical
Publication of ATE495436T1 publication Critical patent/ATE495436T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Peptides Or Proteins (AREA)
AT02731818T 2001-05-14 2002-05-14 Verfahren zur isolierung und markierung von probenmolekülen ATE495436T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/858,198 US7183116B2 (en) 2001-05-14 2001-05-14 Methods for isolation and labeling of sample molecules
PCT/US2002/015500 WO2002093131A2 (en) 2001-05-14 2002-05-14 Methods for isolating and labeling sample molecules

Publications (1)

Publication Number Publication Date
ATE495436T1 true ATE495436T1 (de) 2011-01-15

Family

ID=25327723

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02731818T ATE495436T1 (de) 2001-05-14 2002-05-14 Verfahren zur isolierung und markierung von probenmolekülen

Country Status (9)

Country Link
US (3) US7183116B2 (de)
EP (1) EP1456632B1 (de)
JP (2) JP4414654B2 (de)
CN (1) CN1602422B (de)
AT (1) ATE495436T1 (de)
AU (1) AU2002303760B2 (de)
CA (1) CA2447874C (de)
DE (1) DE60238940D1 (de)
WO (1) WO2002093131A2 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
ES2308800T3 (es) 1997-01-08 2008-12-01 Invitrogen Corporation Metodos para la produccion de proteinas.
JP2003532900A (ja) * 2000-05-05 2003-11-05 パーデュー・リサーチ・ファウンデーション タンパク質を同定及び定量化するための親和性により選択されるサインペプチド
US20030017507A1 (en) * 2001-05-30 2003-01-23 Johnson Richard S. Relative protein quantitation phosphoproteins using stable isotope labeling
AU2002367839A1 (en) 2001-07-16 2003-11-17 Hk Pharmaceuticals, Inc. Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
WO2003027682A2 (en) * 2001-09-27 2003-04-03 Purdue Research Foundation Controlling isotope effects during fractionation of analytes
DE60231845D1 (de) * 2001-12-11 2009-05-14 Univ New Jersey Med Diagnoseverfahren für proteinprofilierung
AU2003202026A1 (en) 2002-01-16 2003-09-02 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
AU2003224674A1 (en) * 2002-03-11 2003-09-29 Hk Pharmaceuticals, Inc. Compounds and methods for analyzing the proteome
US8669116B2 (en) * 2002-03-11 2014-03-11 President And Fellows Of Harvard College Detection and quantification of modified proteins
US20070269895A1 (en) * 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
CA2487314A1 (en) * 2002-06-03 2003-12-11 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
JP2004170195A (ja) * 2002-11-19 2004-06-17 Reverse Proteomics Research Institute Co Ltd タンパク質の固定化方法
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
SG153699A1 (en) * 2003-01-16 2009-07-29 Caprotec Bioanalytics Gmbh Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
WO2005007677A2 (en) * 2003-07-10 2005-01-27 The Institute For Systems Biology Affinity capture of peptides by microarray and related methods
WO2005007663A2 (en) * 2003-07-14 2005-01-27 Irm Llc Fluorogenic enzyme substrates and uses thereof
WO2005007678A2 (en) * 2003-07-14 2005-01-27 Irm Llc Methods for the preparation of rhodamine
WO2005015239A2 (en) * 2003-08-07 2005-02-17 Cornell Research Foundation, Inc. Method for n-terminal labeling of proteins
JP4363935B2 (ja) * 2003-09-17 2009-11-11 株式会社豊田中央研究所 生体物質の光固定化・回収方法及び表面プラズモン共鳴センサー
US7781173B2 (en) * 2003-09-25 2010-08-24 Life Technologies Corporation Homogeneous populations of molecules
US20070048752A1 (en) * 2004-07-12 2007-03-01 Applera Corporation Mass tags for quantitative analyses
US20060172319A1 (en) * 2004-07-12 2006-08-03 Applera Corporation Mass tags for quantitative analyses
WO2006011673A1 (ja) * 2004-07-30 2006-02-02 Reverse Proteomics Research Institute Co., Ltd. 光切断型リンカーを利用したリガンド固定化固相担体
JP4635532B2 (ja) * 2004-09-14 2011-02-23 株式会社ニコン 生体分子の分析および回収方法
US20060134694A1 (en) * 2004-12-22 2006-06-22 Intel Corporation Methods of protein profiling by thiolation
US20060199280A1 (en) * 2005-03-03 2006-09-07 David Bar-Or Quantification of proteins
US8148676B2 (en) * 2005-06-07 2012-04-03 Centre National De La Recherche Scientifique (Cnrs) Use of ionic matrices for maldi mass spectrometry analysis of tissue sections
GB0515323D0 (en) * 2005-07-26 2005-08-31 Electrophoretics Ltd Mass labels
US7906341B2 (en) * 2006-06-30 2011-03-15 Dh Technologies Development Pte, Ltd. Methods, mixtures, kits and compositions pertaining to analyte determination
EP2069386A4 (de) 2006-07-21 2009-10-28 Life Technologies Corp Proteinmolekülgewichtsstandards mit hoher trennschärfe
AU2007349186B2 (en) * 2006-12-07 2014-03-06 Applied Isotope Technologies, Inc Solid phase and catalyzed enabled automated isotope dilution and speciated isotope dilution mass spectrometry
US7728287B2 (en) * 2007-03-01 2010-06-01 Lawrence Livermore National Security, Llc Imaging mass spectrometer with mass tags
WO2011010964A1 (en) * 2009-07-22 2011-01-27 Agency For Science, Technology And Research Differentiation of isobaric amino acids and other species
EP2609426B1 (de) * 2010-08-27 2018-12-19 The Texas A&M University System Fluoreszenzmarkierungsreagenzien und deren verwendungen
US8492163B2 (en) 2011-01-31 2013-07-23 Dh Technologies Development Pte. Ltd. Methods, mixtures, kits and compositions pertaining to analyte determination
GB2528643A (en) * 2014-07-08 2016-02-03 Adc Biotechnology Ltd Method of synthesising ADCs
JP2017020896A (ja) * 2015-07-10 2017-01-26 国立大学法人北陸先端科学技術大学院大学 Ｎ末端標識剤、これを用いた蛍光標識タンパク質及びその製造方法
HK1249092A1 (zh) * 2015-07-29 2018-10-26 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. 用於样品制备尤其是用於质谱分析的方式和方法
CN110687297B (zh) * 2018-07-06 2023-08-22 复旦大学 基于稳定同位素半胱氨酸代谢标记的棕榈酰化修饰蛋白质定量分析方法
GB201913598D0 (en) * 2019-09-20 2019-11-06 Univ Birmingham Labelling of biomolecules
CN113075114B (zh) * 2019-12-17 2022-07-01 北京大学 一种用于单细胞分析的有机质谱流式分析方法
WO2022082098A1 (en) * 2020-10-16 2022-04-21 Lmx Medtech Llc Oxidative mass labeling
CN119569971B (zh) * 2025-02-07 2025-05-27 吉林大学 一种用于蛋白质磷酸化和棕榈酰化修饰同时筛选的Janus材料的制备方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5986076A (en) * 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
CA2193228A1 (en) 1994-06-23 1996-01-04 Christopher Holmes Photolabile compounds and methods for their use
AU734636B2 (en) 1997-07-11 2001-06-21 Xzillion Gmbh & Co. Kg Characterising nucleic acids
ATE253126T1 (de) * 1998-08-25 2003-11-15 Univ Washington Schnelle quantitative analyse von proteinen oder proteinfunktionen in komplexen gemischen
EP1295123A4 (de) 2000-06-12 2005-02-02 Univ Washington Selektives markieren und isolieren von phosphopeptiden und anwendungen in proteomischen analysen
WO2002048717A2 (en) 2000-10-23 2002-06-20 Genetics Institute, Llc. Acid-labile isotope-coded extractant (alice) in mass spectrometric analysis

Also Published As

Publication number Publication date
CN1602422B (zh) 2011-06-08
US7183116B2 (en) 2007-02-27
US20020168644A1 (en) 2002-11-14
AU2002303760B2 (en) 2008-04-10
DE60238940D1 (de) 2011-02-24
US20040110186A1 (en) 2004-06-10
JP4414654B2 (ja) 2010-02-10
US7364911B2 (en) 2008-04-29
CA2447874C (en) 2010-08-31
WO2002093131A3 (en) 2004-05-27
EP1456632A2 (de) 2004-09-15
JP2005503540A (ja) 2005-02-03
EP1456632A4 (de) 2005-04-20
CN1602422A (zh) 2005-03-30
JP2009175157A (ja) 2009-08-06
US20040265810A1 (en) 2004-12-30
CA2447874A1 (en) 2002-11-21
WO2002093131A2 (en) 2002-11-21
EP1456632B1 (de) 2011-01-12

Similar Documents

Publication Publication Date Title
ATE495436T1 (de) Verfahren zur isolierung und markierung von probenmolekülen
DE60010058D1 (de) Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen
PT990047E (pt) Metodos e composicoes para a analise de acidos nucleicos por espectroscopia de massa.
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
SE9803838D0 (sv) A chromatography method and a column material useful in said method
WO2004086050A3 (en) Labelling agents for mass spectrometry comprising tertiary amines
DE69900462D1 (de) Konjugate von dns und pns und verfahren zu deren herstellung
CY1111278T1 (el) Συστημα ασφαλειας
DK2385141T3 (da) Flerkomponentnukleinsyreenzymer og fremgangsmåder til anvendelse heraf
ATE419629T1 (de) Poröse ferro- oder ferrimagnetische glasteilchen für molekültrennung
ATE191934T1 (de) Verfahren zur isothermer amplifikation von nukleinsäure molekülen
ATE443071T1 (de) Verfahren zum entschützen von oligonukleotiden
DE69937447D1 (de) Verfahren zur erkennung von nukleinsäuren
NO20014641L (no) Spaltning av nukleinsyre fra faste b¶rere
AU3143897A (en) Oligonucleotides which specifically bind retroviral nucleocapsid proteins
DE60303020D1 (de) Verfahren zur gewinnung von nukleinsäuren mit hilfe chaotroper-und ammoniumreagentien
EP1983052A3 (de) Verfahren zum Klonen und zur Herstellung von Fragmentketten mit lesbarem Informationsinhalt
BR0213779A (pt) Molécula de ácido nucleico isolada, vetor, célula hospedeira, e, método para a preparação de um composto de quìmica fina
WO2003025567A3 (de) Herstellung von trägergebundenen molekülen mittels oligonucleotid-tags
EP1083980A4 (de) Modulation molekularer wechselwirkungspositionen in rns und anderen biomolekülen
WO2006079099A3 (en) Method of conjugating aminothiol containing molecules to a polymer
ATE514083T1 (de) Verfahren und zusammensetzungen zur reinheitsbestimmung und reinigung chemisch synthetisierter nukleinsäuren
SE9904222L (sv) Nya funktionaliserade polymera reagens
DE59814284D1 (de) Nucleinsäure-sequenzen und verfahren zum nachweis
AU1144699A (en) Group of nucleic acid molecules for (salmonella) detection, nucleic acids, kit and use

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties