WO2003025567A3 - Herstellung von trägergebundenen molekülen mittels oligonucleotid-tags - Google Patents

Herstellung von trägergebundenen molekülen mittels oligonucleotid-tags Download PDF

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Publication number
WO2003025567A3
WO2003025567A3 PCT/DE2002/003414 DE0203414W WO03025567A3 WO 2003025567 A3 WO2003025567 A3 WO 2003025567A3 DE 0203414 W DE0203414 W DE 0203414W WO 03025567 A3 WO03025567 A3 WO 03025567A3
Authority
WO
WIPO (PCT)
Prior art keywords
support
molecules
production
tags
assignment
Prior art date
Application number
PCT/DE2002/003414
Other languages
English (en)
French (fr)
Other versions
WO2003025567A2 (de
Inventor
Stefan Duebel
Original Assignee
Bernard Andre
Stefan Duebel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bernard Andre, Stefan Duebel filed Critical Bernard Andre
Priority to AU2002340734A priority Critical patent/AU2002340734A1/en
Priority to DE10294286T priority patent/DE10294286D2/de
Publication of WO2003025567A2 publication Critical patent/WO2003025567A2/de
Publication of WO2003025567A3 publication Critical patent/WO2003025567A3/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Abstract

Die Erfindung beschreibt Träger für Bindungsassays, bei dem die Zuordnung und Kopplung von Fängermolekülen an die Festphase durch einfaches Überschichten einer in bestimmter Weise vorbehandelten Fläche (Träger) mit einer Lösung geschieht, welche ein Gemisch speziell derivatisierter 'Targets' (Fängermoleküle) enthält. Die Zuordnung eines bestimmten Moleküls zu einem bestimmten Platz auf dem Träger wird durch ein System von Markierungsmolekülen ('Tags') gewährleistet, welche von in bestimmter Form angeordneten Rezeptormolekülen auf dem Träger erkannt wird. Diese 'Tags' und ihre Rezeptormoleküle sind typischerweise Nukleinsäurestücke, können aber auch andere Molekülklassen sein, insbesondere Leucin-zipper, Antikörper-Antigen-Paare oder andere Proteinpaare. Die Erfindung beschreibt weiterhin Verfahren zur Herstellung solcher Träger und der Fängermoleküle, sowie deren Anwendung nach der Kopplung.
PCT/DE2002/003414 2001-09-13 2002-09-13 Herstellung von trägergebundenen molekülen mittels oligonucleotid-tags WO2003025567A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002340734A AU2002340734A1 (en) 2001-09-13 2002-09-13 Production of support-bonded molecules by means of oligonucleotide tags
DE10294286T DE10294286D2 (de) 2001-09-13 2002-09-13 Herstellung von trägergebundenen Molekülen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10145226.8 2001-09-13
DE2001145226 DE10145226A1 (de) 2001-09-13 2001-09-13 Herstellung von trägergebundenen Molekülen

Publications (2)

Publication Number Publication Date
WO2003025567A2 WO2003025567A2 (de) 2003-03-27
WO2003025567A3 true WO2003025567A3 (de) 2003-11-13

Family

ID=7698955

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/003414 WO2003025567A2 (de) 2001-09-13 2002-09-13 Herstellung von trägergebundenen molekülen mittels oligonucleotid-tags

Country Status (3)

Country Link
AU (1) AU2002340734A1 (de)
DE (2) DE10145226A1 (de)
WO (1) WO2003025567A2 (de)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US9096951B2 (en) 2003-02-21 2015-08-04 Nuevolution A/S Method for producing second-generation library
US9109248B2 (en) 2002-10-30 2015-08-18 Nuevolution A/S Method for the synthesis of a bifunctional complex
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL163822A0 (en) 2002-03-15 2005-12-18 Nuevolution As An improved method for synthesising templated molecules
EP1539980B1 (de) 2002-08-01 2016-02-17 Nuevolution A/S MEHRSTUFENSYNTHESE VON MIT MATRIZEN ASSOZIIERTE MOLEKüLEN
WO2004074501A2 (en) * 2003-02-21 2004-09-02 Nuevolution A/S A method for obtaining structural information about an encoded molecule
WO2005026387A1 (en) * 2003-09-18 2005-03-24 Nuevolution A/S A method for obtaining structural information concerning an encoded molecule and method for selecting compounds
DK2558577T3 (en) 2010-04-16 2019-04-01 Nuevolution As Bi-functional complexes and methods for the preparation and use of such complexes

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WO1998054312A1 (en) * 1997-05-28 1998-12-03 Babraham Institute Ribosome complexes as selection particles for in vitro display and evolution of proteins
WO1999051773A1 (en) * 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
WO2000004382A1 (en) * 1998-07-14 2000-01-27 Zyomyx, Inc. Arrays of proteins and methods of use thereof
WO2002014860A1 (en) * 2000-08-15 2002-02-21 Discerna Limited Functional protein arrays

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DE19629141A1 (de) * 1996-07-19 1998-04-16 Bayer Ag Verfahren und Vorrichtung zum Screening von Molekülen bezüglich ihres individuellen Bindungsverhaltens zu mindestens einem vorgegebenen Ligand
US6261804B1 (en) * 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
CA2350417C (en) * 1998-12-02 2010-02-09 Phylos, Inc. Dna-protein fusions and uses thereof
DE19957827C2 (de) * 1999-11-25 2003-06-12 Epigenomics Ag Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche
WO2001053539A1 (en) * 2000-01-24 2001-07-26 Phylos, Inc. Sensitive, multiplexed diagnostic assays for protein analysis

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998054312A1 (en) * 1997-05-28 1998-12-03 Babraham Institute Ribosome complexes as selection particles for in vitro display and evolution of proteins
WO1999051773A1 (en) * 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
WO2000004382A1 (en) * 1998-07-14 2000-01-27 Zyomyx, Inc. Arrays of proteins and methods of use thereof
WO2000004389A2 (en) * 1998-07-14 2000-01-27 Zyomyx, Inc. Arrays of protein-capture agents and methods of use thereof
WO2002014860A1 (en) * 2000-08-15 2002-02-21 Discerna Limited Functional protein arrays

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HE M ET AL: "Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method)", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 29, no. 15, 1 August 2001 (2001-08-01), pages E73, XP002182708, ISSN: 0305-1048 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US9109248B2 (en) 2002-10-30 2015-08-18 Nuevolution A/S Method for the synthesis of a bifunctional complex
US9284600B2 (en) 2002-10-30 2016-03-15 Neuvolution A/S Method for the synthesis of a bifunctional complex
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9096951B2 (en) 2003-02-21 2015-08-04 Nuevolution A/S Method for producing second-generation library
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Also Published As

Publication number Publication date
DE10294286D2 (de) 2004-12-23
AU2002340734A1 (en) 2003-04-01
WO2003025567A2 (de) 2003-03-27
DE10145226A1 (de) 2003-04-10

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