BR0009395A - Método para separar uma molécula de ácidonucléico de um suporte sólido ao qual ela estáligada, de inversamente imobilizar uma moléculade ácido nucléico, de preparar um construto paraligação a, e clivagem subsequente de, um suportesólido, molécula quimérica, método para separaruma célula alvo de uma amostra, kit para uso emum método, poli ou oligonucleotìdeo, e,multiplicidade de oligo ou polinucleotìdeos - Google Patents

Método para separar uma molécula de ácidonucléico de um suporte sólido ao qual ela estáligada, de inversamente imobilizar uma moléculade ácido nucléico, de preparar um construto paraligação a, e clivagem subsequente de, um suportesólido, molécula quimérica, método para separaruma célula alvo de uma amostra, kit para uso emum método, poli ou oligonucleotìdeo, e,multiplicidade de oligo ou polinucleotìdeos

Info

Publication number
BR0009395A
BR0009395A BR0009395-5A BR0009395A BR0009395A BR 0009395 A BR0009395 A BR 0009395A BR 0009395 A BR0009395 A BR 0009395A BR 0009395 A BR0009395 A BR 0009395A
Authority
BR
Brazil
Prior art keywords
nucleic acid
acid molecule
separating
solid support
molecule
Prior art date
Application number
BR0009395-5A
Other languages
English (en)
Portuguese (pt)
Inventor
Andrew Goldsborough
Original Assignee
Andrew Goldsborough
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Andrew Goldsborough filed Critical Andrew Goldsborough
Publication of BR0009395A publication Critical patent/BR0009395A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BR0009395-5A 1999-03-29 2000-03-28 Método para separar uma molécula de ácidonucléico de um suporte sólido ao qual ela estáligada, de inversamente imobilizar uma moléculade ácido nucléico, de preparar um construto paraligação a, e clivagem subsequente de, um suportesólido, molécula quimérica, método para separaruma célula alvo de uma amostra, kit para uso emum método, poli ou oligonucleotìdeo, e,multiplicidade de oligo ou polinucleotìdeos BR0009395A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9907245.6A GB9907245D0 (en) 1999-03-29 1999-03-29 Cleavage of nucleic acids from solid supports
PCT/GB2000/001190 WO2000058329A1 (en) 1999-03-29 2000-03-28 Cleavage of nucleic acid from solid supports

Publications (1)

Publication Number Publication Date
BR0009395A true BR0009395A (pt) 2001-12-26

Family

ID=10850600

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0009395-5A BR0009395A (pt) 1999-03-29 2000-03-28 Método para separar uma molécula de ácidonucléico de um suporte sólido ao qual ela estáligada, de inversamente imobilizar uma moléculade ácido nucléico, de preparar um construto paraligação a, e clivagem subsequente de, um suportesólido, molécula quimérica, método para separaruma célula alvo de uma amostra, kit para uso emum método, poli ou oligonucleotìdeo, e,multiplicidade de oligo ou polinucleotìdeos

Country Status (13)

Country Link
EP (1) EP1165585A1 (no)
JP (1) JP2003500013A (no)
KR (1) KR20020013517A (no)
CN (1) CN1345328A (no)
AU (1) AU3566800A (no)
BR (1) BR0009395A (no)
CA (1) CA2368420A1 (no)
GB (1) GB9907245D0 (no)
IL (1) IL145411A0 (no)
MX (1) MXPA01009698A (no)
NO (1) NO20014641L (no)
NZ (1) NZ514852A (no)
WO (1) WO2000058329A1 (no)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
JP4518754B2 (ja) * 2003-06-30 2010-08-04 パナソニック株式会社 ヌクレオチド鎖修飾方法
WO2005014808A1 (ja) * 2003-06-30 2005-02-17 Matsushita Electric Industrial Co., Ltd. ヌクレオチド鎖修飾方法
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
SG171914A1 (en) 2008-12-02 2011-07-28 Chiralgen Ltd Method for the synthesis of phosphorus atom modified nucleic acids
AU2010270714B2 (en) 2009-07-06 2015-08-13 Wave Life Sciences Ltd. Novel nucleic acid prodrugs and methods use thereof
WO2011108682A1 (ja) * 2010-03-05 2011-09-09 国立大学法人 東京大学 リボヌクレオシドホスホロチオエートの製造方法
US10428019B2 (en) 2010-09-24 2019-10-01 Wave Life Sciences Ltd. Chiral auxiliaries
DK2734208T3 (en) 2011-07-19 2017-06-19 Wave Life Sciences Ltd PROCEDURES FOR SYNTHESIS OF FUNCTIONALIZED NUCLEIC ACIDS
SG11201500232UA (en) 2012-07-13 2015-04-29 Wave Life Sciences Pte Ltd Chiral control
JP6246121B2 (ja) 2012-07-13 2017-12-13 株式会社新日本科学 キラル核酸アジュバント
US9598458B2 (en) 2012-07-13 2017-03-21 Wave Life Sciences Japan, Inc. Asymmetric auxiliary group
EP3095460A4 (en) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
JPWO2015108047A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤
JPWO2015108048A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤
US10160969B2 (en) 2014-01-16 2018-12-25 Wave Life Sciences Ltd. Chiral design
US20170022546A1 (en) * 2014-03-10 2017-01-26 Rashid Bashir Detection and quantification of methylation in dna
GB201717446D0 (en) 2017-10-24 2017-12-06 Evox Therapeutics Ltd Affinity purification of engineering extracellular vesicles
CN109956987B (zh) * 2017-12-14 2022-05-17 南京金斯瑞生物科技有限公司 氨解方法及氨解组合物

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5367066A (en) * 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
SE9003743D0 (sv) * 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers

Also Published As

Publication number Publication date
NZ514852A (en) 2003-07-25
EP1165585A1 (en) 2002-01-02
IL145411A0 (en) 2002-06-30
AU3566800A (en) 2000-10-16
CA2368420A1 (en) 2000-10-05
JP2003500013A (ja) 2003-01-07
NO20014641L (no) 2001-11-27
MXPA01009698A (es) 2003-06-24
GB9907245D0 (en) 1999-05-26
CN1345328A (zh) 2002-04-17
KR20020013517A (ko) 2002-02-20
WO2000058329A1 (en) 2000-10-05
NO20014641D0 (no) 2001-09-25

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1925 DE 27/11/2007.