AU2005245948B2 - Use of whole blood in PCR reactions - Google Patents
Use of whole blood in PCR reactions Download PDFInfo
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- AU2005245948B2 AU2005245948B2 AU2005245948A AU2005245948A AU2005245948B2 AU 2005245948 B2 AU2005245948 B2 AU 2005245948B2 AU 2005245948 A AU2005245948 A AU 2005245948A AU 2005245948 A AU2005245948 A AU 2005245948A AU 2005245948 B2 AU2005245948 B2 AU 2005245948B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
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- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
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|---|---|---|---|
| US10/850,816 | 2004-05-20 | ||
| US10/850,816 US20050260606A1 (en) | 2004-05-20 | 2004-05-20 | Use of whole blood in PCR reactions |
| US11/005,559 US7462475B2 (en) | 2004-05-20 | 2004-12-06 | Use of whole blood in PCR reactions |
| US11/005,559 | 2004-12-06 | ||
| PCT/US2005/017669 WO2005113829A2 (en) | 2004-05-20 | 2005-05-20 | Use of whole blood in pcr reactions |
Publications (2)
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| AU2005245948A1 AU2005245948A1 (en) | 2005-12-01 |
| AU2005245948B2 true AU2005245948B2 (en) | 2011-04-07 |
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| EP (1) | EP1747290B1 (https=) |
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| AU (1) | AU2005245948B2 (https=) |
| CA (1) | CA2567620C (https=) |
| DK (1) | DK1747290T3 (https=) |
| WO (1) | WO2005113829A2 (https=) |
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| US7462475B2 (en) | 2004-05-20 | 2008-12-09 | Dna Poleymerase Technology, Inc. | Use of whole blood in PCR reactions |
| US8632970B2 (en) | 2005-05-09 | 2014-01-21 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
| US8628918B2 (en) * | 2005-05-09 | 2014-01-14 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
| WO2006124771A2 (en) * | 2005-05-12 | 2006-11-23 | Panomics, Inc. | Multiplex branched-chain dna assays |
| ES2511218T5 (es) * | 2005-06-20 | 2017-12-07 | Advanced Cell Diagnostics, Inc. | Kits y productos para detección de ácidos nucleicos en células individuales y para identificación de células raras en grandes poblaciones de células heterogéneas |
| WO2007044427A2 (en) * | 2005-10-05 | 2007-04-19 | Panomics, Inc. | Detection of nucleic acids from whole blood |
| AU2007296180A1 (en) | 2006-09-14 | 2008-03-20 | Dna Polymerase Technology Inc. | Use of Taq polymerase mutant enzymes for DNA amplification in the presence of PCR inhibitors |
| JP5292722B2 (ja) * | 2007-05-09 | 2013-09-18 | 凸版印刷株式会社 | 未精製血液をサンプルとする核酸増幅法 |
| WO2009140497A1 (en) * | 2008-05-16 | 2009-11-19 | New England Biolabs, Inc. | Enzyme reagents for amplification of polynucleotides in the presence of inhibitors |
| FI20095729A0 (fi) * | 2009-06-26 | 2009-06-26 | Finnzymes Oy | Menetelmä deoksiribonukleiinihappojen kvantitatiiviseen PCR-monistukseen PCR-inhibiittoreita sisältävästä näytteestä |
| CN104849472A (zh) | 2010-10-21 | 2015-08-19 | 领先细胞医疗诊断有限公司 | 用于原位检测核酸的超灵敏方法 |
| WO2012054639A2 (en) | 2010-10-22 | 2012-04-26 | T2 Biosystems, Inc. | Nmr systems and methods for the rapid detection of analytes |
| US8563298B2 (en) | 2010-10-22 | 2013-10-22 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
| US8409807B2 (en) | 2010-10-22 | 2013-04-02 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
| EP2697372B1 (en) | 2011-04-11 | 2015-12-16 | Roche Diagnostics GmbH | Dna polymerases with improved activity |
| GB201113430D0 (en) | 2011-08-03 | 2011-09-21 | Fermentas Uab | DNA polymerases |
| US11208636B2 (en) | 2011-08-10 | 2021-12-28 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
| CN103857805B (zh) | 2011-08-10 | 2017-07-18 | 生命技术公司 | 聚合酶组合物、制备和使用所述聚合酶组合物的方法 |
| JP6144697B2 (ja) | 2011-12-08 | 2017-06-07 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
| US9562271B2 (en) | 2012-04-20 | 2017-02-07 | T2 Biosystems, Inc. | Compositions and methods for detection of Candida species |
| EP3366769B1 (en) | 2012-10-16 | 2021-02-17 | DNA Polymerase Technology, Inc. | Inhibition-resistant polymerases |
| GB201219137D0 (en) * | 2012-10-24 | 2012-12-05 | Ge Healthcare Uk Ltd | Direct nucleic acid amplification kit, reagent and method |
| EP3248011A4 (en) | 2015-01-21 | 2018-11-14 | T2 Biosystems, Inc. | Nmr methods and systems for the rapid detection of tick-borne pathogens |
| US11091745B2 (en) | 2015-05-12 | 2021-08-17 | Dna Polymerase Technology, Inc. | Mutant polymerases and uses thereof |
| DK3362462T3 (da) | 2015-10-12 | 2021-10-11 | Advanced Cell Diagnostics Inc | In situ-detektion af nukleotidvarianter i prøver med højt støjniveau, og sammensætninger og fremgangsmåder relateret dertil |
| US11046939B2 (en) | 2015-11-27 | 2021-06-29 | Kyushu University, National University Corporation | DNA polymerase variant |
| WO2017127731A1 (en) | 2016-01-21 | 2017-07-27 | T2 Biosystems, Inc. | Nmr methods and systems for the rapid detection of bacteria |
| US20190032104A1 (en) | 2016-01-21 | 2019-01-31 | T2 Biosystems, Inc. | Rapid antimicrobial susceptibility testing using high-sensitivity direct detection methods |
| WO2017180745A1 (en) | 2016-04-14 | 2017-10-19 | T2 Biosystems, Inc. | Methods and systems for amplification in complex samples |
| US10626444B2 (en) * | 2016-10-14 | 2020-04-21 | ACUDX Inc. | Methods of assaying nucleic acids of low quantities using a buffer nucleic acid |
| KR102313431B1 (ko) | 2016-11-21 | 2021-10-18 | 나노스트링 테크놀로지스, 인크. | 화학적 조성물 및 이것을 사용하는 방법 |
| US10961518B2 (en) | 2018-01-19 | 2021-03-30 | Bio-Rad Laboratories, Inc. | Mutant DNA polymerases |
| AU2019271028B2 (en) | 2018-05-14 | 2025-09-18 | Bruker Spatial Biology, Inc. | Chemical compositions and methods of using same |
| CN109022386B (zh) * | 2018-07-12 | 2020-11-03 | 深圳市华中生物药械有限公司 | 重组Taq直扩酶及其制备方法、重组质粒和工程菌 |
| US20220090194A1 (en) * | 2019-01-09 | 2022-03-24 | 3Cr Bioscience Ltd. | Method for detecting a nucleic acid sequence |
| GB2587177A (en) * | 2019-01-09 | 2021-03-24 | 3Cr Bioscience Ltd | Polymerase |
| CN110295237B (zh) * | 2019-06-27 | 2023-07-25 | 河北农业大学 | 一种改进的鸡隐性白羽基因型的分子鉴定方法 |
| US20230304082A1 (en) * | 2020-05-29 | 2023-09-28 | Genotech Corp. | Dna polymerase variant with improved discrimination of genetic variants |
| CN114134220A (zh) * | 2022-01-30 | 2022-03-04 | 济凡生物科技（北京）有限公司 | 一种可以用于血液检测的pcr反应液及其试剂盒 |
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| US5466591A (en) * | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| DE553264T1 (de) * | 1990-10-05 | 1994-04-28 | Wayne M Barnes | Thermostabile dna polymerase. |
| DK0590327T3 (da) * | 1992-09-11 | 2003-07-28 | Hoffmann La Roche | Påvisning af nukleinsyrer i blod |
| US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US6818431B1 (en) | 1995-10-18 | 2004-11-16 | Shanghai Mendel Dna Center Co. Ltd. | DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides |
| CA2326725A1 (en) * | 1998-04-23 | 1999-10-28 | Takara Shuzo Co., Ltd. | Method for synthesizing dna |
| US6214557B1 (en) * | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
| US6428962B1 (en) * | 2001-02-12 | 2002-08-06 | Dna Analysis, Inc. | Nucleic acid collection barrier method and apparatus |
| US6896872B2 (en) | 2001-06-27 | 2005-05-24 | Svetlana A. Dambinova | Rapid multiple panel of biomarkers in laboratory blood tests for TIA/stroke |
| US20060234227A1 (en) * | 2002-05-14 | 2006-10-19 | Alexei Slesarev | Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes |
| US7462475B2 (en) | 2004-05-20 | 2008-12-09 | Dna Poleymerase Technology, Inc. | Use of whole blood in PCR reactions |
| AU2007296180A1 (en) | 2006-09-14 | 2008-03-20 | Dna Polymerase Technology Inc. | Use of Taq polymerase mutant enzymes for DNA amplification in the presence of PCR inhibitors |
-
2004
- 2004-12-06 US US11/005,559 patent/US7462475B2/en not_active Expired - Lifetime
-
2005
- 2005-05-20 EP EP05759554.8A patent/EP1747290B1/en not_active Expired - Lifetime
- 2005-05-20 DK DK05759554.8T patent/DK1747290T3/da active
- 2005-05-20 JP JP2007527461A patent/JP2008506417A/ja active Pending
- 2005-05-20 CA CA2567620A patent/CA2567620C/en not_active Expired - Lifetime
- 2005-05-20 AU AU2005245948A patent/AU2005245948B2/en not_active Expired - Fee Related
- 2005-05-20 WO PCT/US2005/017669 patent/WO2005113829A2/en not_active Ceased
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2008
- 2008-12-08 US US12/330,201 patent/US8470563B2/en not_active Expired - Fee Related
-
2013
- 2013-06-01 US US13/907,891 patent/US20130252309A1/en not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| Kermekchiev, MB et al., Nucleic Acids Research, vol 31, pages 6139-6147 * |
Also Published As
| Publication number | Publication date |
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| WO2005113829A2 (en) | 2005-12-01 |
| EP1747290A2 (en) | 2007-01-31 |
| CA2567620C (en) | 2016-09-20 |
| JP2008506417A (ja) | 2008-03-06 |
| WO2005113829A3 (en) | 2008-04-03 |
| EP1747290B1 (en) | 2014-05-07 |
| US8470563B2 (en) | 2013-06-25 |
| US20060084074A1 (en) | 2006-04-20 |
| US20130252309A1 (en) | 2013-09-26 |
| AU2005245948A1 (en) | 2005-12-01 |
| DK1747290T3 (da) | 2014-08-11 |
| CA2567620A1 (en) | 2005-12-01 |
| US7462475B2 (en) | 2008-12-09 |
| EP1747290A4 (en) | 2009-01-14 |
| US20090170060A1 (en) | 2009-07-02 |
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