CN106701716B - 一种热不稳定的ung酶及其应用 - Google Patents
一种热不稳定的ung酶及其应用 Download PDFInfo
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- CN106701716B CN106701716B CN201610873765.5A CN201610873765A CN106701716B CN 106701716 B CN106701716 B CN 106701716B CN 201610873765 A CN201610873765 A CN 201610873765A CN 106701716 B CN106701716 B CN 106701716B
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- ung enzyme
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
- C12Y302/02027—Uracil-DNA glycosylase (3.2.2.27)
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- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
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- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
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Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610873765.5A CN106701716B (zh) | 2016-10-08 | 2016-10-08 | 一种热不稳定的ung酶及其应用 |
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CN201610873765.5A CN106701716B (zh) | 2016-10-08 | 2016-10-08 | 一种热不稳定的ung酶及其应用 |
Publications (2)
Publication Number | Publication Date |
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CN106701716A CN106701716A (zh) | 2017-05-24 |
CN106701716B true CN106701716B (zh) | 2020-08-11 |
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ID=58940673
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Application Number | Title | Priority Date | Filing Date |
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CN201610873765.5A Active CN106701716B (zh) | 2016-10-08 | 2016-10-08 | 一种热不稳定的ung酶及其应用 |
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CN (1) | CN106701716B (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107828758A (zh) * | 2017-11-13 | 2018-03-23 | 江苏众红生物工程创药研究院有限公司 | 重组尿嘧啶‑dna糖苷酶及其编码基因、制备方法和应用 |
KR102169528B1 (ko) * | 2019-08-06 | 2020-10-23 | 주식회사 엔지노믹스 | 열민감성이 향상된 돌연변이 udg |
EP4008779A4 (en) * | 2020-10-22 | 2023-07-19 | Enzynomics Co. Ltd. | UDG MUTANTE FEATURING ENHANCED THERMAL SENSITIVITY |
CN114854723A (zh) * | 2022-05-26 | 2022-08-05 | 中国科学院分子植物科学卓越创新中心 | 水稻尿嘧啶dna糖苷酶及其在基因编辑诱导植物单碱基多样性中的应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6187575B1 (en) * | 1995-12-05 | 2001-02-13 | Roche Diagnostics Gmbh | Thermolabile uracil-DNA-glycosylas, process for its preparation and use for removing uracil from DNA |
CN1325989A (zh) * | 2000-05-26 | 2001-12-12 | 上海博德基因开发有限公司 | 一种新的多肽——人尿嘧啶脱氧核糖核酸糖基化酶22和编码这种多肽的多核苷酸 |
US6713294B1 (en) * | 1996-01-09 | 2004-03-30 | Hans E. Krokan | DNA glycosylases and their use |
KR20090039531A (ko) * | 2007-10-18 | 2009-04-22 | 성균관대학교산학협력단 | 바실러스 스피시스 нj171 균주 유래의 신규한 저온성우라실-dna 글리코실라제의 제조방법 및 중합효소연쇄반응에서의 이용 |
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2016
- 2016-10-08 CN CN201610873765.5A patent/CN106701716B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6187575B1 (en) * | 1995-12-05 | 2001-02-13 | Roche Diagnostics Gmbh | Thermolabile uracil-DNA-glycosylas, process for its preparation and use for removing uracil from DNA |
US6713294B1 (en) * | 1996-01-09 | 2004-03-30 | Hans E. Krokan | DNA glycosylases and their use |
CN1325989A (zh) * | 2000-05-26 | 2001-12-12 | 上海博德基因开发有限公司 | 一种新的多肽——人尿嘧啶脱氧核糖核酸糖基化酶22和编码这种多肽的多核苷酸 |
KR20090039531A (ko) * | 2007-10-18 | 2009-04-22 | 성균관대학교산학협력단 | 바실러스 스피시스 нj171 균주 유래의 신규한 저온성우라실-dna 글리코실라제의 제조방법 및 중합효소연쇄반응에서의 이용 |
Non-Patent Citations (3)
Title |
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Heat-labile uracil-DNA glycosylase: purification and characterization;SobeK H等;《FEBS Lett》;19960610;第388卷(第1期);全文 * |
Use of heat labile UNG in an RT-PCR assay for enterovirus detection;Taggart EW等;《Journal of virological methods》;20020831;第105卷(第1期);全文 * |
尿嘧啶DNA糖基化酶在PCR防污染中的应用;李振勇等;《中国医药导刊》;20041123;第3卷(第4期);全文 * |
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CN106701716A (zh) | 2017-05-24 |
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Effective date of registration: 20181204 Address after: 102200 No. 2M-10/12, No. 2 Building, No. 36 Zhenxing Road, Changping District Science and Technology Park, Beijing Applicant after: Beijing Tianyue Oriental Gene Technology Co.,Ltd. Address before: Dawa Port-vicinity Economic Zone, Panjin City, Liaoning Province Applicant before: LIAONING ERKANG GENE TECHNOLOGY CO.,LTD. |
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Address after: No.2m-10 and No.12, building 2, No.36, Zhenxing Road, science and Technology Park, Changping District, Beijing Applicant after: Blue dream times (Beijing) Biotechnology Co.,Ltd. Address before: No.2m-10 / 12, building 2, No.36, Zhenxing Road, science and Technology Park, Changping District Applicant before: Beijing Tianyue Oriental Gene Technology Co.,Ltd. |
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Effective date of registration: 20220712 Address after: Room 203, floor 2, building 23, yard 79, Shuangying West Road, Changping District Science Park, Beijing 102200 Patentee after: Beijing lingfu Biotechnology Co.,Ltd. Address before: No.2m-10, 12, building 2, No.36, Zhenxing Road, science and Technology Park, Changping District, Beijing Patentee before: Blue dream times (Beijing) Biotechnology Co.,Ltd. |
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