JP2016535987A5 - - Google Patents
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- JP2016535987A5 JP2016535987A5 JP2016524530A JP2016524530A JP2016535987A5 JP 2016535987 A5 JP2016535987 A5 JP 2016535987A5 JP 2016524530 A JP2016524530 A JP 2016524530A JP 2016524530 A JP2016524530 A JP 2016524530A JP 2016535987 A5 JP2016535987 A5 JP 2016535987A5
- Authority
- JP
- Japan
- Prior art keywords
- dna sequence
- sequence
- blocker
- hybridizes
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 44
- 238000000034 method Methods 0.000 claims 24
- 230000000295 complement effect Effects 0.000 claims 14
- 239000011541 reaction mixture Substances 0.000 claims 13
- 210000001124 body fluid Anatomy 0.000 claims 5
- 239000010839 body fluid Substances 0.000 claims 5
- 238000001816 cooling Methods 0.000 claims 4
- 238000010438 heat treatment Methods 0.000 claims 4
- 238000002844 melting Methods 0.000 claims 4
- 230000008018 melting Effects 0.000 claims 4
- 102100030708 GTPase KRas Human genes 0.000 claims 3
- 101000584612 Homo sapiens GTPase KRas Proteins 0.000 claims 3
- -1 c- MET Proteins 0.000 claims 3
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 claims 3
- 108700015053 epidermal growth factor receptor activity proteins Proteins 0.000 claims 3
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 210000002700 urine Anatomy 0.000 claims 3
- 108091093088 Amplicon Proteins 0.000 claims 2
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- 101000605639 Homo sapiens Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Proteins 0.000 claims 2
- 101000984753 Homo sapiens Serine/threonine-protein kinase B-raf Proteins 0.000 claims 2
- 102100038332 Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Human genes 0.000 claims 2
- 102100027103 Serine/threonine-protein kinase B-raf Human genes 0.000 claims 2
- 108010078814 Tumor Suppressor Protein p53 Proteins 0.000 claims 2
- 210000002381 plasma Anatomy 0.000 claims 2
- 238000012163 sequencing technique Methods 0.000 claims 2
- 210000002966 serum Anatomy 0.000 claims 2
- 101100322915 Caenorhabditis elegans akt-1 gene Proteins 0.000 claims 1
- ZEOWTGPWHLSLOG-UHFFFAOYSA-N Cc1ccc(cc1-c1ccc2c(n[nH]c2c1)-c1cnn(c1)C1CC1)C(=O)Nc1cccc(c1)C(F)(F)F Chemical compound Cc1ccc(cc1-c1ccc2c(n[nH]c2c1)-c1cnn(c1)C1CC1)C(=O)Nc1cccc(c1)C(F)(F)F ZEOWTGPWHLSLOG-UHFFFAOYSA-N 0.000 claims 1
- 102100023593 Fibroblast growth factor receptor 1 Human genes 0.000 claims 1
- 101710182386 Fibroblast growth factor receptor 1 Proteins 0.000 claims 1
- 102100023600 Fibroblast growth factor receptor 2 Human genes 0.000 claims 1
- 101710182389 Fibroblast growth factor receptor 2 Proteins 0.000 claims 1
- 102100027842 Fibroblast growth factor receptor 3 Human genes 0.000 claims 1
- 101710182396 Fibroblast growth factor receptor 3 Proteins 0.000 claims 1
- 102100039788 GTPase NRas Human genes 0.000 claims 1
- 101000744505 Homo sapiens GTPase NRas Proteins 0.000 claims 1
- 101001012157 Homo sapiens Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 claims 1
- 101000829171 Hypocrea virens (strain Gv29-8 / FGSC 10586) Effector TSP1 Proteins 0.000 claims 1
- 102100025744 Mothers against decapentaplegic homolog 1 Human genes 0.000 claims 1
- 108010051742 Platelet-Derived Growth Factor beta Receptor Proteins 0.000 claims 1
- 102100026547 Platelet-derived growth factor receptor beta Human genes 0.000 claims 1
- 102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 claims 1
- 101700032040 SMAD1 Proteins 0.000 claims 1
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- 230000037429 base substitution Effects 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 230000001351 cycling effect Effects 0.000 claims 1
- 238000007847 digital PCR Methods 0.000 claims 1
- 238000012165 high-throughput sequencing Methods 0.000 claims 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 claims 1
- 238000011880 melting curve analysis Methods 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 238000012175 pyrosequencing Methods 0.000 claims 1
- 238000003753 real-time PCR Methods 0.000 claims 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361893283P | 2013-10-20 | 2013-10-20 | |
| US201361904141P | 2013-11-14 | 2013-11-14 | |
| US61/904,141 | 2013-11-14 | ||
| US201462039905P | 2014-08-20 | 2014-08-20 | |
| US62/039,905 | 2014-08-20 | ||
| US61/893,283 | 2014-10-20 | ||
| PCT/US2014/061435 WO2015073163A2 (en) | 2013-11-14 | 2014-10-20 | Synthesis and enrichment of nucleic acid sequences |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016535987A JP2016535987A (ja) | 2016-11-24 |
| JP2016535987A5 true JP2016535987A5 (https=) | 2017-11-30 |
Family
ID=55151140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016524530A Withdrawn JP2016535987A (ja) | 2013-10-20 | 2014-10-20 | 核酸配列の合成及び濃縮 |
Country Status (3)
| Country | Link |
|---|---|
| US (2) | US20160273022A1 (https=) |
| JP (1) | JP2016535987A (https=) |
| CN (1) | CN105283555A (https=) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4414990A3 (en) | 2013-01-17 | 2024-11-06 | Personalis, Inc. | Methods and systems for genetic analysis |
| US10125399B2 (en) | 2014-10-30 | 2018-11-13 | Personalis, Inc. | Methods for using mosaicism in nucleic acids sampled distal to their origin |
| PL3298162T3 (pl) * | 2015-05-18 | 2020-07-27 | Saga Diagnostics Ab | Wykrywanie docelowego kwasu nukleinowego i wariantów |
| US11299783B2 (en) | 2016-05-27 | 2022-04-12 | Personalis, Inc. | Methods and systems for genetic analysis |
| CN107012221A (zh) * | 2016-10-14 | 2017-08-04 | 苏州艾达康医疗科技有限公司 | 基于阻断物的富集系统及其应用 |
| CN106755451A (zh) * | 2017-01-05 | 2017-05-31 | 苏州艾达康医疗科技有限公司 | 核酸制备及分析 |
| WO2019022132A1 (ja) * | 2017-07-26 | 2019-01-31 | 積水メディカル株式会社 | 変異遺伝子検出方法 |
| CN108410982A (zh) * | 2018-02-05 | 2018-08-17 | 广州市达瑞生物技术股份有限公司 | 一种基于maldi-tof-ms检测肺癌34个突变位点的试剂盒 |
| CN108179191A (zh) * | 2018-02-05 | 2018-06-19 | 广州市达瑞生物技术股份有限公司 | 一种检测人类ctDNA基因突变的试剂盒 |
| US10801064B2 (en) | 2018-05-31 | 2020-10-13 | Personalis, Inc. | Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples |
| US11814750B2 (en) | 2018-05-31 | 2023-11-14 | Personalis, Inc. | Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples |
| CN109632938B (zh) * | 2019-01-11 | 2021-03-16 | 中国科学院化学研究所 | 聚多巴胺修饰的银纳米颗粒在质谱分析检测中的应用 |
| CN109762901B (zh) * | 2019-01-30 | 2022-11-29 | 杭州瑞普基因科技有限公司 | 用于富集低频dna突变的dna探针应用于多种突变的同时检测 |
| JP7470787B2 (ja) | 2019-11-05 | 2024-04-18 | パーソナリス，インコーポレイティド | 単一試料からの腫瘍純度の推定 |
| CN111004839A (zh) * | 2019-12-18 | 2020-04-14 | 杭州瑞普基因科技有限公司 | 基于锁核酸修饰的阻滞物进行阻滞取代扩增富集检测目标突变的方法 |
| WO2023059654A1 (en) | 2021-10-05 | 2023-04-13 | Personalis, Inc. | Customized assays for personalized cancer monitoring |
| CN115287338A (zh) * | 2022-07-04 | 2022-11-04 | 苏州唯善生物科技有限公司 | 一种提高数字pcr灵敏度的检测方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5849497A (en) * | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| US6391592B1 (en) * | 2000-12-14 | 2002-05-21 | Affymetrix, Inc. | Blocker-aided target amplification of nucleic acids |
| AUPS076902A0 (en) | 2002-02-26 | 2002-03-21 | Commonwealth Scientific And Industrial Research Organisation | Novel selective polymerase chain reaction |
| ES2546848T3 (es) * | 2006-03-10 | 2015-09-29 | Epigenomics Ag | Un método para identificar una muestra biológica para el análisis de la metilación |
| JP5531367B2 (ja) * | 2007-08-01 | 2014-06-25 | ダナ−ファーバー キャンサー インスチテュート | 標的配列の濃縮 |
| EP2853601B1 (en) | 2008-07-18 | 2016-09-21 | TrovaGene, Inc. | Methods for PCR-based detection of "ultra short" nucleic acid sequences |
| US20130178383A1 (en) * | 2008-11-12 | 2013-07-11 | David Spetzler | Vesicle isolation methods |
| US8206929B2 (en) | 2009-01-07 | 2012-06-26 | Roche Molecular Systems, Inc. | Nucleic acid amplification with allele-specific suppression of sequence variants |
| AU2011224534B2 (en) | 2010-03-08 | 2014-10-09 | Dana-Farber Cancer Institute, Inc. | Full COLD-PCR enrichment with reference blocking sequence |
| KR101312241B1 (ko) | 2010-04-27 | 2013-09-27 | 사회복지법인 삼성생명공익재단 | 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법 |
| CN103459612A (zh) | 2010-12-03 | 2013-12-18 | 布兰代斯大学 | 用于检测野生型群体中的核酸突变体的方法和试剂盒 |
| GB201100150D0 (en) | 2011-01-06 | 2011-02-23 | Epistem Ltd | Mutational analysis |
| US20140147846A1 (en) | 2011-05-26 | 2014-05-29 | Brandeis University | Detection of sequence variants in the human epidermal growth factor receptor (egfr) gene |
| US9133490B2 (en) | 2012-05-16 | 2015-09-15 | Transgenomic, Inc. | Step-up method for COLD-PCR enrichment |
-
2014
- 2014-10-20 CN CN201480013111.4A patent/CN105283555A/zh active Pending
- 2014-10-20 US US15/027,688 patent/US20160273022A1/en not_active Abandoned
- 2014-10-20 JP JP2016524530A patent/JP2016535987A/ja not_active Withdrawn
-
2016
- 2016-09-03 US US15/256,523 patent/US9725755B2/en not_active Expired - Fee Related
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