JP2014526899A5 - - Google Patents

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JP2014526899A5
JP2014526899A5 JP2014527291A JP2014527291A JP2014526899A5 JP 2014526899 A5 JP2014526899 A5 JP 2014526899A5 JP 2014527291 A JP2014527291 A JP 2014527291A JP 2014527291 A JP2014527291 A JP 2014527291A JP 2014526899 A5 JP2014526899 A5 JP 2014526899A5
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nucleic acid
stranded nucleic
acid fragments
blunt
double stranded
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JP2014527291A
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JP2014526899A (ja
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Priority claimed from US13/592,827 external-priority patent/US20130059296A1/en
Publication of JP2014526899A publication Critical patent/JP2014526899A/ja
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JP2014527291A 2011-08-26 2012-08-23 核酸の高忠実度アセンブリのための組成物および方法 Pending JP2014526899A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201161527922P 2011-08-26 2011-08-26
US61/527,922 2011-08-26
US201161532825P 2011-09-09 2011-09-09
US61/532,825 2011-09-09
US13/592,827 2012-08-23
US13/592,827 US20130059296A1 (en) 2011-08-26 2012-08-23 Compositions and Methods For High Fidelity Assembly of Nucleic Acids
PCT/US2012/052036 WO2013032850A2 (en) 2011-08-26 2012-08-23 Compositions and methods for high fidelity assembly of nucleic acids

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JP2017038387A Division JP2017136070A (ja) 2011-08-26 2017-03-01 核酸の高忠実度アセンブリのための組成物および方法

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JP2014526899A JP2014526899A (ja) 2014-10-09
JP2014526899A5 true JP2014526899A5 (OSRAM) 2015-10-08

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JP2014527291A Pending JP2014526899A (ja) 2011-08-26 2012-08-23 核酸の高忠実度アセンブリのための組成物および方法
JP2017038387A Pending JP2017136070A (ja) 2011-08-26 2017-03-01 核酸の高忠実度アセンブリのための組成物および方法
JP2020136799A Active JP7208956B2 (ja) 2011-08-26 2020-08-13 核酸の高忠実度アセンブリのための組成物および方法

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JP2017038387A Pending JP2017136070A (ja) 2011-08-26 2017-03-01 核酸の高忠実度アセンブリのための組成物および方法
JP2020136799A Active JP7208956B2 (ja) 2011-08-26 2020-08-13 核酸の高忠実度アセンブリのための組成物および方法

Country Status (10)

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US (3) US20130059296A1 (OSRAM)
EP (4) EP2944693B1 (OSRAM)
JP (3) JP2014526899A (OSRAM)
CN (1) CN104066844B (OSRAM)
AU (2) AU2012300401B2 (OSRAM)
DK (1) DK3594340T3 (OSRAM)
ES (1) ES2737957T3 (OSRAM)
IL (4) IL280334B2 (OSRAM)
LT (2) LT3594340T (OSRAM)
WO (1) WO2013032850A2 (OSRAM)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
LT3360963T (lt) 2010-11-12 2020-02-25 Gen9, Inc. Nukleorūgščių sintezės būdai ir įrenginiai
EP4039363A1 (en) 2010-11-12 2022-08-10 Gen9, Inc. Protein arrays and methods of using and making the same
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
LT3594340T (lt) 2011-08-26 2021-10-25 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN104603286B (zh) 2012-04-24 2020-07-31 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
EP3483311B8 (en) 2012-06-25 2025-10-29 Ginkgo Bioworks, Inc. Methods for nucleic acid assembly and high throughput sequencing
US10280417B2 (en) 2013-03-13 2019-05-07 Gen 9, Inc. Compositions, methods and apparatus for oligonucleotides synthesis
WO2014160059A1 (en) * 2013-03-13 2014-10-02 Gen9, Inc. Compositions and methods for synthesis of high fidelity oligonucleotides
CN105209642A (zh) * 2013-03-15 2015-12-30 卡耐基华盛顿学院 基因组测序和表观遗传分析的方法
CN108467863B (zh) * 2013-03-15 2022-01-25 Gen9股份有限公司 多重核酸合成的组合物和方法
EP3030682B1 (en) 2013-08-05 2020-06-03 Twist Bioscience Corporation De novo synthesized gene libraries
US20170175110A1 (en) * 2013-11-27 2017-06-22 Gen9, Inc. Libraries of Nucleic Acids and Methods for Making the Same
SG11201610168YA (en) 2014-05-16 2017-01-27 Illumina Inc Nucleic acid synthesis techniques
WO2016007604A1 (en) 2014-07-09 2016-01-14 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
WO2016197374A1 (zh) * 2015-06-11 2016-12-15 深圳华大基因研究院 寡核苷酸及其应用
US20180202072A1 (en) * 2015-07-20 2018-07-19 The Regents Of The University Of California Sequence-independent nucleic acid assembly
CN106435744B (zh) * 2015-08-13 2022-02-01 生捷科技控股公司 使用y-接头和消失限制位点的文库构建
JP6982362B2 (ja) 2015-09-18 2021-12-17 ツイスト バイオサイエンス コーポレーション オリゴ核酸変異体ライブラリーとその合成
KR20250053972A (ko) 2015-09-22 2025-04-22 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
EP3384077A4 (en) 2015-12-01 2019-05-08 Twist Bioscience Corporation FUNCTIONALIZED SURFACES AND MANUFACTURE THEREOF
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
CN105950609A (zh) * 2016-04-28 2016-09-21 苏州泓迅生物科技有限公司 半导体芯片高通量合成核苷酸池及其组装双链dna的方法
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation NOVO SYNTHESIZED NUCLEIC ACID BANKS
US20180105806A1 (en) * 2016-09-07 2018-04-19 Massachusetts Institute Of Technology Method for rna-guided endonuclease-based dna assembly
WO2018057526A2 (en) 2016-09-21 2018-03-29 Twist Bioscience Corporation Nucleic acid based data storage
EP3554514A4 (en) 2016-12-16 2020-08-05 Twist Bioscience Corporation BANKS OF VARIANTS OF IMMUNOLOGICAL SYNAPSE AND THEIR SYNTHESIS
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
US10894959B2 (en) 2017-03-15 2021-01-19 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN110691868A (zh) * 2017-05-22 2020-01-14 Gen9股份有限公司 用于核酸操纵的装置和方法
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
SG11201912057RA (en) 2017-06-12 2020-01-30 Twist Bioscience Corp Methods for seamless nucleic acid assembly
JP2020536504A (ja) 2017-09-11 2020-12-17 ツイスト バイオサイエンス コーポレーション Gpcr結合タンパク質およびその合成
GB2583590A (en) 2017-10-20 2020-11-04 Twist Bioscience Corp Heated nanowells for polynucleotide synthesis
GB2585506A (en) 2018-01-04 2021-01-13 Twist Bioscience Corp DNA-based digital information storage
CN118957038A (zh) 2018-05-18 2024-11-15 特韦斯特生物科学公司 用于核酸杂交的多核苷酸、试剂和方法
EP3867373A1 (en) * 2018-10-19 2021-08-25 New England Biolabs, Inc. Improved ordered assembly of multiple dna fragments
US12188011B2 (en) 2018-10-19 2025-01-07 New England Biolabs, Inc. Compositions and methods for improved in vitro assembly of polynucleotides
US12435332B2 (en) 2018-10-19 2025-10-07 New England Biolabs, Inc. Ordered assembly of multiple DNA fragments
CN113692409B (zh) 2018-12-26 2025-01-10 特韦斯特生物科学公司 高度准确的从头多核苷酸合成
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
AU2020229349A1 (en) 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
GB201905303D0 (en) * 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
EP3987019A4 (en) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
EP4034566A4 (en) 2019-09-23 2024-01-24 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CRTH2
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
US20240025939A1 (en) * 2020-03-06 2024-01-25 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
US11530406B1 (en) 2021-08-30 2022-12-20 Sachi Bioworks Inc. System and method for producing a therapeutic oligomer

Family Cites Families (385)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4500707A (en) 1980-02-29 1985-02-19 University Patents, Inc. Nucleosides useful in the preparation of polynucleotides
US5096825A (en) 1983-01-12 1992-03-17 Chiron Corporation Gene for human epidermal growth factor and synthesis and expression thereof
DE3301833A1 (de) 1983-01-20 1984-07-26 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur simultanen synthese mehrerer oligonocleotide an fester phase
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US4888286A (en) 1984-02-06 1989-12-19 Creative Biomolecules, Inc. Production of gene and protein analogs through synthetic gene design using double stranded synthetic oligonucleotides
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5093251A (en) 1986-05-23 1992-03-03 California Institute Of Technology Cassette method of gene synthesis
US5017692A (en) 1986-09-04 1991-05-21 Schering Corporation Truncated human interleukin-a alpha
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US4999294A (en) 1987-12-17 1991-03-12 New England Biolabs, Inc. Method for producing the FokI restriction endonuclease and methylase
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
EP0427745A4 (en) 1988-07-14 1992-11-25 Baylor College Of Medicine Solid phase assembly and reconstruction of biopolymers
US5132215A (en) 1988-09-15 1992-07-21 Eastman Kodak Company Method of making double-stranded dna sequences
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5047524A (en) 1988-12-21 1991-09-10 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US6008031A (en) 1989-05-12 1999-12-28 Duke University Method of analysis and manipulation of DNA utilizing mismatch repair systems
US5459039A (en) 1989-05-12 1995-10-17 Duke University Methods for mapping genetic mutations
US5556750A (en) 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US5104789A (en) 1989-05-15 1992-04-14 The United States Of America, As Represented By The Secretary Of Agriculture Monoclonal antibodies which discriminate between strains of citrus tristeza virus
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US5514568A (en) * 1991-04-26 1996-05-07 Eli Lilly And Company Enzymatic inverse polymerase chain reaction
US5512463A (en) 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US5639603A (en) 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
EP0773227A1 (en) 1991-09-18 1997-05-14 Affymax Technologies N.V. Diverse collections of oligomers in use to prepare drugs, diagnostic reagents, pesticides or herbicides
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US6017696A (en) 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
EP0675966B1 (en) 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5395750A (en) 1992-02-28 1995-03-07 Hoffmann-La Roche Inc. Methods for producing proteins which bind to predetermined antigens
US5916794A (en) 1992-04-03 1999-06-29 Johns Hopkins University Methods for inactivating target DNA and for detecting conformational change in a nucleic acid
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
GB9207381D0 (en) 1992-04-03 1992-05-13 Ici Plc Synthesis of oligonucleotides
WO1993022457A1 (en) 1992-04-24 1993-11-11 Massachusetts Institute Of Technology Screening for genetic variation
US5541061A (en) 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5288514A (en) 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5368823A (en) 1993-02-11 1994-11-29 University Of Georgia Research Foundation, Inc. Automated synthesis of oligonucleotides
US6150141A (en) 1993-09-10 2000-11-21 Trustees Of Boston University Intron-mediated recombinant techniques and reagents
US5498531A (en) 1993-09-10 1996-03-12 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
US6027877A (en) 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
KR100491810B1 (ko) 1994-02-17 2005-10-11 맥시겐, 인크. 무작위단편화및재조립에의한dna돌연변이유발방법
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5514789A (en) 1994-04-21 1996-05-07 Barrskogen, Inc. Recovery of oligonucleotides by gas phase cleavage
DE69531545T2 (de) 1994-06-23 2004-06-24 Affymax Technologies N.V., Willemstad Photolabile verbindungen und verfahren für deren verwendung
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5871902A (en) 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
CA2215417A1 (en) 1995-03-15 1996-09-19 City Of Hope Disposable reagent storage and delivery cartridge
CA2219136A1 (en) * 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5877280A (en) 1995-06-06 1999-03-02 The Mount Sinai School Of Medicine Of The City University Of New York Thermostable muts proteins
US6406847B1 (en) 1995-10-02 2002-06-18 The Board Of Trustees Of The Leland Stanford Junior University Mismatch repair detection
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
ATE236270T1 (de) 1995-12-15 2003-04-15 Univ Duke Verfahren zum nachweis und zur entfernung von mutantsequenzen, die während enzymatischer amplifikation entstehen
US6261797B1 (en) 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US5851804A (en) 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
SE9602062D0 (sv) 1996-05-29 1996-05-29 Pharmacia Biotech Ab Method for detection of mutations
US6495318B2 (en) 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
IL127624A0 (en) 1996-06-17 1999-10-28 Biodynamics Associates Method and kits for preparing multicomponent nucleic acid constructs
ZA975891B (en) 1996-07-05 1998-07-23 Combimatrix Corp Electrochemical solid phase synthesis of polymers
JPH1066576A (ja) * 1996-08-07 1998-03-10 Novo Nordisk As 突出末端を有する2本鎖dna及びこれを用いたdnaのシャフリング方法
WO1998007846A1 (en) 1996-08-23 1998-02-26 Peter Ruhdal Jensen Artificial promoter libraries for selected organisms and promoters derived from such libraries
US6444650B1 (en) 1996-10-01 2002-09-03 Geron Corporation Antisense compositions for detecting and inhibiting telomerase reverse transcriptase
US6110668A (en) 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
AU5156198A (en) 1996-10-29 1998-05-22 Xeotron Corporation Optical device utilizing optical waveguides and mechanical light-switches
ATE259056T1 (de) 1996-11-06 2004-02-15 Sequenom Inc Verfahren zur massenspektrometrie
IL120338A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Single step DNA fragments assembly
IL120339A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
WO1998038326A1 (en) 1997-02-28 1998-09-03 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
US6410225B1 (en) 1997-06-27 2002-06-25 Yale University Purification of oligomers
US6489141B1 (en) 1997-07-09 2002-12-03 The University Of Queensland Nucleic acid sequence and methods for selectively expressing a protein in a target cell or tissue
NZ501917A (en) 1997-07-22 2001-10-26 Qiagen Genomics Inc Method and apparatus for enzymatic reactions performed on nucleic acid arrays conducted at dew point
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
US6031098A (en) 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
DE19736591A1 (de) 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
US6333153B1 (en) 1997-08-28 2001-12-25 Thomas Jefferson University Compositions, kits, and methods for effecting adenine nucleotide modulation of DNA mismatch recognition proteins
US6136568A (en) 1997-09-15 2000-10-24 Hiatt; Andrew C. De novo polynucleotide synthesis using rolling templates
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
ATE294860T1 (de) 1997-09-16 2005-05-15 Egea Biosciences Llc Methoden zur kompletten chemischen synthese und zusammensetzung von genen und genomen
US20020127552A1 (en) 1997-10-10 2002-09-12 Church George M Replica amplification of nucleic acid arrays
US6548021B1 (en) 1997-10-10 2003-04-15 President And Fellows Of Harvard College Surface-bound, double-stranded DNA protein arrays
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
US6177558B1 (en) 1997-11-13 2001-01-23 Protogene Laboratories, Inc. Method and composition for chemical synthesis using high boiling point organic solvents to control evaporation
US6042211A (en) 1997-11-25 2000-03-28 Hewlett-Packard Company Ink drop volume variance compensation for inkjet printing
AU1603199A (en) 1997-12-03 1999-06-16 Curagen Corporation Methods and devices for measuring differential gene expression
ATE242320T1 (de) 1997-12-05 2003-06-15 Europ Lab Molekularbiolog Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems
DE07008633T1 (de) 1997-12-11 2008-12-18 Merial Virus des multisystemischen Kümmersyndroms bei Absatzferkeln
US20030050438A1 (en) 1998-01-05 2003-03-13 Montgomery Donald D. Electrochemical solid phase synthesis
US6093302A (en) 1998-01-05 2000-07-25 Combimatrix Corporation Electrochemical solid phase synthesis
US6150102A (en) 1998-02-03 2000-11-21 Lucent Technologies Inc. Method of generating nucleic acid oligomers of known composition
DE69909972T2 (de) 1998-02-11 2004-05-13 University Of Houston, Houston Vorrichtung zur durchführung chemischer und biochemischer reaktionen unter anwendung von photoerzeugten reagenzien
EP2259046A3 (en) 1998-02-23 2011-11-30 Wisconsin Alumni Research Foundation Method for synthesis of arrays of dna probes
US5912129A (en) 1998-03-05 1999-06-15 Vinayagamoorthy; Thuraiayah Multi-zone polymerase/ligase chain reaction
DE19812103A1 (de) 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
US6670605B1 (en) 1998-05-11 2003-12-30 Halliburton Energy Services, Inc. Method and apparatus for the down-hole characterization of formation fluids
US6271957B1 (en) 1998-05-29 2001-08-07 Affymetrix, Inc. Methods involving direct write optical lithography
EP0967217B1 (en) 1998-06-22 2005-12-21 Affymetrix, Inc. (a California Corporation) Reagents and methods for solid phase synthesis and display
US6846655B1 (en) 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
US6287825B1 (en) 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
DE19854946C2 (de) 1998-11-27 2002-01-03 Guenter Von Kiedrowski Klonieren und Kopieren an Oberflächen
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
US20040005673A1 (en) 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
ATE439437T1 (de) * 1999-01-05 2009-08-15 Univ Boston Verbessertes klonierungsverfahren
US20030054390A1 (en) 1999-01-19 2003-03-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6372484B1 (en) 1999-01-25 2002-04-16 E.I. Dupont De Nemours And Company Apparatus for integrated polymerase chain reaction and capillary electrophoresis
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
EP1147209A2 (en) 1999-02-03 2001-10-24 The Children's Medical Center Corporation Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site
US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
ATE334197T1 (de) 1999-02-19 2006-08-15 Febit Biotech Gmbh Verfahren zur herstellung von polymeren
DE60020340T2 (de) 1999-03-08 2006-03-16 Metrigen, Inc., Burlingame Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür
US6511849B1 (en) 1999-04-23 2003-01-28 The Sir Mortimer B. Davis - Jewish General Hospital Microarrays of biological materials
US6824866B1 (en) 1999-04-08 2004-11-30 Affymetrix, Inc. Porous silica substrates for polymer synthesis and assays
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
DE19925862A1 (de) 1999-06-07 2000-12-14 Diavir Gmbh Verfahren zur Synthese von DNA-Fragmenten
US6355412B1 (en) 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
US6653151B2 (en) 1999-07-30 2003-11-25 Large Scale Proteomics Corporation Dry deposition of materials for microarrays using matrix displacement
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
GB9920711D0 (en) 1999-09-03 1999-11-03 Hd Technologies Limited High dynamic range mass spectrometer
EP1235932A2 (en) 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US6315958B1 (en) 1999-11-10 2001-11-13 Wisconsin Alumni Research Foundation Flow cell for synthesis of arrays of DNA probes and the like
US6800439B1 (en) 2000-01-06 2004-10-05 Affymetrix, Inc. Methods for improved array preparation
EP1130113A1 (en) 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
WO2001062968A2 (en) 2000-02-25 2001-08-30 General Atomics Mutant nucleic binding enzymes and use thereof in diagnostic, detection and purification methods
US6833450B1 (en) 2000-03-17 2004-12-21 Affymetrix, Inc. Phosphite ester oxidation in nucleic acid array preparation
US6365355B1 (en) 2000-03-28 2002-04-02 The Regents Of The University Of California Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
US6969587B2 (en) 2000-04-04 2005-11-29 Taylor Paul D Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
US20020025561A1 (en) 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
WO2001081568A1 (en) * 2000-04-21 2001-11-01 Genencor International, Inc. Non-pcr based recombination of nucleic acids
DE10022995C2 (de) 2000-05-11 2003-11-27 Wavelight Laser Technologie Ag Vorrichtung für die photorefraktive Hornhautchirurgie
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
AU2001264802A1 (en) 2000-05-21 2001-12-03 University Of North Carolina At Chapel Hill Assembly of large viral genomes and chromosomes from subclones
CA2410440A1 (en) 2000-06-02 2001-12-13 Blue Heron Biotechnology, Inc. Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US20030118486A1 (en) 2000-07-03 2003-06-26 Xeotron Corporation Fluidic methods and devices for parallel chemical reactions
CA2415258A1 (en) 2000-07-03 2002-01-10 Xeotron Corporation Devices and methods for carrying out chemical reactions using photogenerated reagents
GB0016472D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
AU2001271893A1 (en) 2000-07-07 2002-01-21 Nimblegen Systems, Inc. Method and apparatus for synthesis of arrays of dna probes
AU2001277014A1 (en) 2000-07-21 2002-02-05 Trustees Of Boston University Modular vector systems
EP1598432A3 (en) 2000-07-31 2006-06-07 Agilent Technologies, Inc. Array based methods for sythesizing nucleic acid mixtures
GB0018876D0 (en) 2000-08-01 2000-09-20 Applied Research Systems Method of producing polypeptides
AU8337701A (en) 2000-08-14 2002-02-25 Us Health Enhanced homologous recombination mediated by lambda recombination proteins
CN1468313A (zh) 2000-08-24 2004-01-14 �����ɷ� 新颖的构建物及其在代谢途径工程中的用途
US6610499B1 (en) 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
US6966945B1 (en) 2000-09-20 2005-11-22 Goodrich Corporation Inorganic matrix compositions, composites and process of making the same
US6666541B2 (en) 2000-09-25 2003-12-23 Picoliter Inc. Acoustic ejection of fluids from a plurality of reservoirs
US20020037359A1 (en) 2000-09-25 2002-03-28 Mutz Mitchell W. Focused acoustic energy in the preparation of peptide arrays
US6658802B2 (en) 2000-10-24 2003-12-09 Northwest Rubber Extruders, Inc. Seal for fixed window of motor vehicle with curved concave corners
EP1205548A1 (en) 2000-11-09 2002-05-15 Kabushiki Kaisha Toyota Chuo Kenkyusho Methods and apparatus for successively ligating double-stranded DNA molecules on a solid phase
US6709861B2 (en) 2000-11-17 2004-03-23 Lucigen Corp. Cloning vectors and vector components
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US6596239B2 (en) 2000-12-12 2003-07-22 Edc Biosystems, Inc. Acoustically mediated fluid transfer methods and uses thereof
US6660475B2 (en) 2000-12-15 2003-12-09 New England Biolabs, Inc. Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof
US20020133359A1 (en) 2000-12-26 2002-09-19 Appareon System, method and article of manufacture for country and regional treatment in a supply chain system
MXPA03006344A (es) 2001-01-19 2004-12-03 Egea Biosciences Inc Montaje dirigido por computadora de un polinucleotido que codifica un polipeptido objetivo.
US6514704B2 (en) 2001-02-01 2003-02-04 Xerox Corporation Quality control mechanism and process for a biofluid multi-ejector system
WO2002077286A1 (en) 2001-02-21 2002-10-03 Gene Check, Inc. Mutation detection using muts and reca
EP1366057B1 (en) 2001-03-08 2005-12-14 Applera Corporation Method for deprotecting oligonucleotides
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
DK1390535T3 (da) 2001-04-26 2010-12-06 Amgen Mountain View Inc Kombinatoriske biblioteker af monomer-domæner
US6951720B2 (en) 2001-05-10 2005-10-04 San Diego State University Foundation Use of phosphorothiolate polynucleotides in ligating nucleic acids
US6558907B2 (en) 2001-05-16 2003-05-06 Corning Incorporated Methods and compositions for arraying nucleic acids onto a solid support
CA2447240C (en) 2001-05-18 2013-02-19 Wisconsin Alumni Research Foundation Method for the synthesis of dna sequences
US20030044980A1 (en) 2001-06-05 2003-03-06 Gorilla Genomics, Inc. Methods for low background cloning of DNA using long oligonucleotides
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
RU2004100107A (ru) 2001-06-08 2005-06-10 Шанхай Мендель Дна Сентер Ко., Лтд (Cn) Низкотемпературное циклическое удлинение днк с высокой специфичностью праймирования
US7179423B2 (en) 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US6416164B1 (en) 2001-07-20 2002-07-09 Picoliter Inc. Acoustic ejection of fluids using large F-number focusing elements
EP1409666B1 (en) * 2001-07-23 2006-03-29 DSM IP Assets B.V. Process for preparing variant polynucleotides
US6734436B2 (en) 2001-08-07 2004-05-11 Sri International Optical microfluidic devices and methods
CA2461372C (en) 2001-09-27 2014-01-07 Bionomics Limited Dna sequences for human angiogenesis genes
WO2003033718A1 (en) 2001-10-17 2003-04-24 Global Genomics Ab Synthesis of oligonucleotides on solid support and assembly into doublestranded polynucleotides
US20030087298A1 (en) 2001-11-02 2003-05-08 Roland Green Detection of hybridization on oligonucleotide microarray through covalently labeling microarray probe
DE50114507D1 (de) 2001-11-22 2009-01-02 Sloning Biotechnology Gmbh Nukleinsäure-Linker und deren Verwendung in der Gensynthese
US20030099952A1 (en) 2001-11-26 2003-05-29 Roland Green Microarrays with visible pattern detection
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
AU2002357249A1 (en) 2001-12-13 2003-07-09 Blue Heron Biotechnology, Inc. Methods for removal of double-stranded oligonucleotides containing sequence errors using mismatch recognition proteins
US20030171325A1 (en) 2002-01-04 2003-09-11 Board Of Regents, The University Of Texas System Proofreading, error deletion, and ligation method for synthesis of high-fidelity polynucleotide sequences
US6897025B2 (en) 2002-01-07 2005-05-24 Perlegen Sciences, Inc. Genetic analysis systems and methods
US20030215837A1 (en) 2002-01-14 2003-11-20 Diversa Corporation Methods for purifying double-stranded nucleic acids lacking base pair mismatches or nucleotide gaps
US20050130156A1 (en) 2002-01-14 2005-06-16 Gerhard Frey Methods for making polynucleotides and purifying double-stranded polynucleotides
US20030219781A1 (en) 2002-01-14 2003-11-27 Diversa Corporation Compositions and methods for making polynucleotides by iterative assembly of codon building blocks
US20040096826A1 (en) 2002-01-30 2004-05-20 Evans Glen A. Methods for creating recombination products between nucleotide sequences
US7037659B2 (en) 2002-01-31 2006-05-02 Nimblegen Systems Inc. Apparatus for constructing DNA probes having a prismatic and kaleidoscopic light homogenizer
US20040126757A1 (en) 2002-01-31 2004-07-01 Francesco Cerrina Method and apparatus for synthesis of arrays of DNA probes
US7422851B2 (en) 2002-01-31 2008-09-09 Nimblegen Systems, Inc. Correction for illumination non-uniformity during the synthesis of arrays of oligomers
US7157229B2 (en) 2002-01-31 2007-01-02 Nimblegen Systems, Inc. Prepatterned substrate for optical synthesis of DNA probes
US7083975B2 (en) 2002-02-01 2006-08-01 Roland Green Microarray synthesis instrument and method
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
EP1483409B1 (en) 2002-02-28 2009-04-15 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
WO2003078659A2 (en) 2002-03-20 2003-09-25 Innovativebio.Biz Microcapsules with controlable permeability encapsulating a nucleic acid amplification reaction mixture and their use as reaction compartments for parallels reactions
US20030182172A1 (en) 2002-03-25 2003-09-25 Claggett Stuart Lee System and method to build project management processes
US7482119B2 (en) 2002-04-01 2009-01-27 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production
EP1499626A2 (en) 2002-04-01 2005-01-26 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production
AU2003228395B2 (en) 2002-04-02 2006-12-21 Caliper Life Sciences, Inc. Methods and apparatus for separation and isolation of components from a biological sample
US20060014146A1 (en) 2002-04-22 2006-01-19 Philippe Soucaille Method of creating a library of bacterial clones with varying levels of gene expression
US20040171047A1 (en) 2002-05-22 2004-09-02 Dahl Gary A. Target-dependent transcription
WO2003100019A2 (en) 2002-05-24 2003-12-04 Invitrogen Corporation Nested pcr employing degradable primers
WO2003100012A2 (en) 2002-05-24 2003-12-04 Nimblegen Systems, Inc. Microarrays and method for running hybridization reaction for multiple samples on a single microarray
US7291460B2 (en) * 2002-05-31 2007-11-06 Verenium Corporation Multiplexed systems for nucleic acid sequencing
US6932097B2 (en) 2002-06-18 2005-08-23 Picoliter Inc. Acoustic control of the composition and/or volume of fluid in a reservoir
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US20040101444A1 (en) 2002-07-15 2004-05-27 Xeotron Corporation Apparatus and method for fluid delivery to a hybridization station
GB0221053D0 (en) 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US20040166567A1 (en) 2002-09-26 2004-08-26 Santi Daniel V Synthetic genes
WO2004029586A1 (en) 2002-09-27 2004-04-08 Nimblegen Systems, Inc. Microarray with hydrophobic barriers
JP4471927B2 (ja) 2002-09-30 2010-06-02 ニンブルゲン システムズ インコーポレイテッド アレイの並列装填方法
US20040110212A1 (en) 2002-09-30 2004-06-10 Mccormick Mark Microarrays with visual alignment marks
WO2004031351A2 (en) 2002-10-01 2004-04-15 Nimblegen Systems, Inc. Microarrays having multiple oligonucleotides in single array features
CA2501857A1 (en) 2002-10-09 2004-04-22 The Board Of Trustees Of The University Of Illinois Microfluidic systems and components
DK1411122T3 (da) 2002-10-18 2008-11-03 Sloning Biotechnology Gmbh Fremgangsmåde til fremstilling af nukleinsyremolekyler
US7267984B2 (en) 2002-10-31 2007-09-11 Rice University Recombination assembly of large DNA fragments
GB0228289D0 (en) * 2002-12-04 2003-01-08 Genome Inst Of Singapore Nat U Method
US7879580B2 (en) 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
US7932025B2 (en) 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
AU2004209426B2 (en) 2003-01-29 2008-04-17 454 Life Sciences Corporation Method for preparing single-stranded DNA libraries
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
WO2004090170A1 (en) 2003-04-02 2004-10-21 Blue Heron Biotechnology, Inc. Error reduction in automated gene synthesis
US7262031B2 (en) 2003-05-22 2007-08-28 The Regents Of The University Of California Method for producing a synthetic gene or other DNA sequence
US20040241655A1 (en) 2003-05-29 2004-12-02 Yuchi Hwang Conditional touchdown multiplex polymerase chain reaction
US8133670B2 (en) 2003-06-13 2012-03-13 Cold Spring Harbor Laboratory Method for making populations of defined nucleic acid molecules
ATE553216T1 (de) 2003-06-20 2012-04-15 Exiqon As Sonden, bibliotheken und kits zur analyse von nukleinsäuregemischen und verfahren zu deren konstruktion
US20050069928A1 (en) 2003-08-04 2005-03-31 Blue Heron Biotechnology, Inc. Methods for synthesis of defined polynucleotides
EP2484687A3 (en) 2003-08-08 2012-11-14 Life Technologies Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US7198900B2 (en) 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20070169227A1 (en) 2003-12-16 2007-07-19 Pioneer Hi-Bred International Inc. Dominant Gene Suppression Transgenes and Methods of Using Same
US7582858B2 (en) 2004-01-23 2009-09-01 Sri International Apparatus and method of moving micro-droplets using laser-induced thermal gradients
DK1557464T3 (da) 2004-01-23 2011-01-24 Sloning Biotechnology Gmbh Enzymatisk fremstilling af nykleinsyremolekylder
AU2005222788A1 (en) 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
US7432055B2 (en) 2004-03-05 2008-10-07 Uchicago Argonne Llc Dual phase multiplex polymerase chain reaction
US20050208503A1 (en) 2004-03-16 2005-09-22 Handy Yowanto Chemical ligation of nucleic acids
US20050227316A1 (en) 2004-04-07 2005-10-13 Kosan Biosciences, Inc. Synthetic genes
US8825411B2 (en) 2004-05-04 2014-09-02 Dna Twopointo, Inc. Design, synthesis and assembly of synthetic nucleic acids
JP2005319407A (ja) 2004-05-10 2005-11-17 Hitachi Ltd 圧電デバイスを用いた機器
EP1604741A1 (en) 2004-05-14 2005-12-14 F. Hoffmann-La Roche Ag Method and apparatus for dispensing a liquid with a pipetting needle
DE602005022443D1 (de) * 2004-06-09 2010-09-02 Wisconsin Alumni Res Found Schnelle synthese von oligonukleotiden
US20060008833A1 (en) 2004-07-12 2006-01-12 Jacobson Joseph M Method for long, error-reduced DNA synthesis
ATE496998T1 (de) 2004-08-27 2011-02-15 Wisconsin Alumni Res Found Verfahren zur fehlerverringerung in nukleinsäurepopulationen
CA2580070A1 (en) 2004-09-10 2006-03-23 Sequenom, Inc. Methods for long-range sequence analysis of nucleic acids
US20060194214A1 (en) 2005-02-28 2006-08-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
CA2584984A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20070122817A1 (en) 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
WO2006049843A1 (en) 2004-11-01 2006-05-11 Parallele Bioscience, Inc. Multiplex polynucleotide synthesis
WO2006053571A2 (en) * 2004-11-22 2006-05-26 Peter Birk Rasmussen Template directed split and mix systhesis of small molecule libraries
US7699979B2 (en) * 2005-01-07 2010-04-20 Board Of Trustees Of The University Of Arkansas Separation system and efficient capture of contaminants using magnetic nanoparticles
CA2594832A1 (en) 2005-01-13 2006-07-20 Codon Devices, Inc. Compositions and methods for protein design
EP2003214B1 (en) 2005-02-01 2013-04-10 AB Advanced Genetic Analysis Corporation Reagents, methods, and libraries for bead-based sequencing
WO2006086209A2 (en) 2005-02-10 2006-08-17 Compass Genetics, Llc Genetic analysis by sequence-specific sorting
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US7285835B2 (en) * 2005-02-24 2007-10-23 Freescale Semiconductor, Inc. Low power magnetoelectronic device structures utilizing enhanced permeability materials
EP1885885A4 (en) 2005-05-11 2008-08-27 Nanolytics Inc METHOD OR DEVICE FOR CONDUCTING CHEMICAL OR BIOCHEMICAL REACTIONS AT MULTIPLE TEMPERATURES
US20060281113A1 (en) 2005-05-18 2006-12-14 George Church Accessible polynucleotide libraries and methods of use thereof
EP1922419A4 (en) 2005-06-10 2010-11-17 Life Technologies Corp METHOD AND SYSTEM FOR GENETIC MULTIPLEX ANALYSIS
EP2302070B1 (en) 2005-06-23 2012-08-22 Keygene N.V. Strategies for high throughput identification and detection of polymorphisms
WO2007005053A1 (en) 2005-06-30 2007-01-11 Codon Devices, Inc. Hierarchical assembly methods for genome engineering
WO2007008951A1 (en) 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for design of non-immunogenic proteins
CA2615057A1 (en) 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for biocatalytic engineering
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
ATE510930T1 (de) * 2005-08-02 2011-06-15 Rubicon Genomics Inc Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion
MY143596A (en) 2005-08-11 2011-06-15 Synthetic Genomics Inc In vitro recombination method
WO2007075438A2 (en) 2005-12-15 2007-07-05 Codon Devices, Inc. Polypeptides comprising unnatural amino acids, methods for their production and uses therefor
US7537897B2 (en) 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
WO2007087347A2 (en) 2006-01-24 2007-08-02 Codon Devices, Inc. Methods, systems, and apparatus for facilitating the design of molecular constructs
JP2009529876A (ja) 2006-03-14 2009-08-27 ゲニゾン バイオサイエンシス インコーポレイテッド 核酸を配列決定するための方法および手段
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
WO2007123742A2 (en) 2006-03-31 2007-11-01 Codon Devices, Inc. Methods and compositions for increasing the fidelity of multiplex nucleic acid assembly
US20070231805A1 (en) 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
EP1842915A1 (en) * 2006-04-04 2007-10-10 Libragen Method of in vitro polynucleotide sequences shuffling by recursive circular DNA molecules fragmentation and ligation
WO2007120624A2 (en) 2006-04-10 2007-10-25 Codon Devices, Inc. Concerted nucleic acid assembly reactions
WO2007136833A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc. Methods and compositions for aptamer production and uses thereof
WO2007136736A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc. Methods for nucleic acid sorting and synthesis
WO2007136835A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc Methods and cells for creating functional diversity and uses thereof
US20070281309A1 (en) 2006-05-19 2007-12-06 Massachusetts Institute Of Technology Microfluidic-based Gene Synthesis
WO2007136834A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2007136840A2 (en) 2006-05-20 2007-11-29 Codon Devices, Inc. Nucleic acid library design and assembly
WO2008054543A2 (en) 2006-05-20 2008-05-08 Codon Devices, Inc. Oligonucleotides for multiplex nucleic acid assembly
ATE496144T1 (de) 2006-06-06 2011-02-15 Gen Probe Inc Markierte oligonukleotide und ihre verwendung in nukleinsäureverstärkungsverfahren
WO2008024319A2 (en) 2006-08-20 2008-02-28 Codon Devices, Inc. Microfluidic devices for nucleic acid assembly
DE102006039479A1 (de) 2006-08-23 2008-03-06 Febit Biotech Gmbh Programmierbare Oligonukleotidsynthese
US8053191B2 (en) * 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US20080287320A1 (en) 2006-10-04 2008-11-20 Codon Devices Libraries and their design and assembly
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US20080214408A1 (en) 2006-10-18 2008-09-04 Government Of The Uinted States Of America, Represented By The Secretary Dept. In situ assembly of protein microarrays
WO2008076368A2 (en) 2006-12-13 2008-06-26 Codon Devices, Inc. Fragment-rearranged nucleic acids and uses thereof
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US8748146B2 (en) 2007-04-19 2014-06-10 Celexion, Llc Engineered nucleases and their uses for nucleic acid assembly
WO2009006445A2 (en) * 2007-06-29 2009-01-08 Applied Biosystems Systems and methods for electronic detection with nanofets
US20090305233A1 (en) 2007-07-03 2009-12-10 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Methods and Reagents for Polynucleotide Assembly
EP2016997B1 (en) 2007-07-10 2011-01-05 Roche Diagnostics GmbH Microfluidic device, method of mixing and use of the device
WO2009032167A1 (en) 2007-08-29 2009-03-12 Illumina Cambridge Method for sequencing a polynucleotide template
KR100957057B1 (ko) 2007-12-03 2010-05-13 래플진(주) 핵산과 신호 프로브의 동시 등온증폭을 이용한 핵산의검출방법
CA2718905A1 (en) * 2008-03-17 2009-09-24 Expressive Research B.V. Expression-linked gene discovery
US9409177B2 (en) 2008-03-21 2016-08-09 Lawrence Livermore National Security, Llc Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences
CN101577391B (zh) 2008-05-07 2011-03-23 富士康(昆山)电脑接插件有限公司 插头连接器及其制造方法
US9068209B2 (en) 2008-05-14 2015-06-30 British Columbia Cancer Agency Branch Gene synthesis by convergent assembly of oligonucleotide subsets
US20100047876A1 (en) * 2008-08-08 2010-02-25 President And Fellows Of Harvard College Hierarchical assembly of polynucleotides
US8808986B2 (en) 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US8999679B2 (en) * 2008-12-18 2015-04-07 Iti Scotland Limited Method for assembly of polynucleic acid sequences
JP5985390B2 (ja) 2009-04-02 2016-09-06 フリューダイム・コーポレイション 標的核酸にバーコードを付加するためのマルチプライマー増幅方法
CN104404134B (zh) 2009-04-03 2017-05-10 莱弗斯基因股份有限公司 多重核酸检测方法和系统
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US20130224729A1 (en) 2009-08-12 2013-08-29 President And Fellows Of Harvard College Biodetection Methods and Compositions
WO2011023800A1 (en) 2009-08-31 2011-03-03 Basf Plant Science Company Gmbh Regulatory nucleic acid molecules for enhancing seed-specific gene expression in plants promoting enhanced polyunsaturated fatty acid synthesis
PL389135A1 (pl) 2009-09-28 2011-04-11 Michał Lower Sposób i sonda DNA do otrzymywania endonukleaz restrukcyjnych, zwłaszcza o pożądanej specyficzności sekwencyjnej
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US20110117559A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011066186A1 (en) * 2009-11-25 2011-06-03 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
US8850219B2 (en) 2010-05-13 2014-09-30 Salesforce.Com, Inc. Secure communications
WO2011143556A1 (en) 2010-05-13 2011-11-17 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) * 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
CN101921840B (zh) 2010-06-30 2014-06-25 深圳华大基因科技有限公司 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法
ES2618127T3 (es) 2010-10-27 2017-06-20 President And Fellows Of Harvard College Composiciones de dúplex de cebador de extremos cohesivos y métodos de uso
LT3360963T (lt) 2010-11-12 2020-02-25 Gen9, Inc. Nukleorūgščių sintezės būdai ir įrenginiai
EP4039363A1 (en) 2010-11-12 2022-08-10 Gen9, Inc. Protein arrays and methods of using and making the same
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
US9809904B2 (en) 2011-04-21 2017-11-07 University Of Washington Through Its Center For Commercialization Methods for retrieval of sequence-verified DNA constructs
EP2530159A1 (en) 2011-06-03 2012-12-05 Sandoz Ag Transcription terminator sequences
CN103732744A (zh) 2011-06-15 2014-04-16 Gen9股份有限公司 制备性体外克隆的方法
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
LT3594340T (lt) 2011-08-26 2021-10-25 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
US20150203839A1 (en) 2011-08-26 2015-07-23 Gen9, Inc. Compositions and Methods for High Fidelity Assembly of Nucleic Acids
US9322023B2 (en) 2011-10-06 2016-04-26 Cornell University Constructs and methods for the assembly of biological pathways
HK1200871A1 (en) 2011-11-16 2015-08-14 Sangamo Therapeutics, Inc. Modified dna-binding proteins and uses thereof
US8939620B2 (en) 2011-12-21 2015-01-27 Kawasaki Jukogyo Kabushiki Kaisha Saddle-riding type automotive vehicle
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US20130281308A1 (en) 2012-04-24 2013-10-24 Gen9, Inc. Methods for sorting nucleic acids and preparative in vitro cloning
CN104603286B (zh) 2012-04-24 2020-07-31 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
EP3483311B8 (en) 2012-06-25 2025-10-29 Ginkgo Bioworks, Inc. Methods for nucleic acid assembly and high throughput sequencing
CN104870635B (zh) 2012-11-30 2020-04-10 艾克瑟根有限公司 具有增加比例的e1b蛋白质的156r剪接同工型的溶瘤腺病毒
KR102479178B1 (ko) 2012-12-06 2022-12-19 시그마-알드리치 컴퍼니., 엘엘씨 Crispr-기초된 유전체 변형과 조절
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
JP6700788B2 (ja) 2012-12-17 2020-05-27 プレジデント アンド フェローズ オブ ハーバード カレッジ Rna誘導性ヒトゲノム改変
WO2014160059A1 (en) 2013-03-13 2014-10-02 Gen9, Inc. Compositions and methods for synthesis of high fidelity oligonucleotides
US10280417B2 (en) 2013-03-13 2019-05-07 Gen 9, Inc. Compositions, methods and apparatus for oligonucleotides synthesis
KR102271292B1 (ko) 2013-03-15 2021-07-02 더 제너럴 하스피탈 코포레이션 Rna-안내 게놈 편집의 특이성을 증가시키기 위한 rna-안내 foki 뉴클레아제(rfn)의 용도
CN108467863B (zh) 2013-03-15 2022-01-25 Gen9股份有限公司 多重核酸合成的组合物和方法
US20140272346A1 (en) 2013-03-15 2014-09-18 Rubicon Technology, Inc. Method of growing aluminum oxide onto substrates by use of an aluminum source in an oxygen environment to create transparent, scratch resistant windows
WO2014191518A1 (en) 2013-05-29 2014-12-04 Cellectis A method for producing precise dna cleavage using cas9 nickase activity
US10421957B2 (en) 2013-07-29 2019-09-24 Agilent Technologies, Inc. DNA assembly using an RNA-programmable nickase
LT3027771T (lt) 2013-07-30 2019-04-25 Gen9, Inc. Ilgos kloninės sekos verifikuotų nukleorūgščių konstruktų gamybos būdas
MX370560B (es) 2013-08-08 2019-12-17 Illumina Inc Sistema de fluidos para distribución de reactivo a una celda de flujo.
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US20170175110A1 (en) 2013-11-27 2017-06-22 Gen9, Inc. Libraries of Nucleic Acids and Methods for Making the Same
WO2016007604A1 (en) 2014-07-09 2016-01-14 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
US20170349925A1 (en) 2014-10-21 2017-12-07 Gingko Bioworks, Inc. Methods for Nucleic Acid Assembly
EP3384022A4 (en) 2015-11-30 2019-07-31 Gen9, Inc. TRANSCRIPTION TERMINATOR AND USE THEREOF
US20190010530A1 (en) 2015-12-21 2019-01-10 Gen9, Inc. Methods and compositions for nucleic acid assembly

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