IL120338A0 - Single step DNA fragments assembly - Google Patents

Single step DNA fragments assembly

Info

Publication number
IL120338A0
IL120338A0 IL12033897A IL12033897A IL120338A0 IL 120338 A0 IL120338 A0 IL 120338A0 IL 12033897 A IL12033897 A IL 12033897A IL 12033897 A IL12033897 A IL 12033897A IL 120338 A0 IL120338 A0 IL 120338A0
Authority
IL
Israel
Prior art keywords
dna fragments
single step
step dna
fragments assembly
assembly
Prior art date
Application number
IL12033897A
Original Assignee
Gesher Israel Advanced Biotecs
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gesher Israel Advanced Biotecs filed Critical Gesher Israel Advanced Biotecs
Priority to IL12033897A priority Critical patent/IL120338A0/en
Publication of IL120338A0 publication Critical patent/IL120338A0/en
Priority to PCT/IL1998/000095 priority patent/WO1998038297A1/en
Priority to PCT/IL1998/000097 priority patent/WO1998038299A1/en
Priority to AU61125/98A priority patent/AU6112598A/en
Priority to AU62287/98A priority patent/AU6228798A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
IL12033897A 1997-02-27 1997-02-27 Single step DNA fragments assembly IL120338A0 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
IL12033897A IL120338A0 (en) 1997-02-27 1997-02-27 Single step DNA fragments assembly
PCT/IL1998/000095 WO1998038297A1 (en) 1997-02-27 1998-02-26 Single step assembly of multiple dna fragments
PCT/IL1998/000097 WO1998038299A1 (en) 1997-02-27 1998-02-26 Single step assembly of multiple dna fragments
AU61125/98A AU6112598A (en) 1997-02-27 1998-02-26 Single step assembly of multiple dna fragments
AU62287/98A AU6228798A (en) 1997-02-27 1998-02-26 Single step assembly of multiple dna fragments

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IL12033897A IL120338A0 (en) 1997-02-27 1997-02-27 Single step DNA fragments assembly

Publications (1)

Publication Number Publication Date
IL120338A0 true IL120338A0 (en) 1997-06-10

Family

ID=11069866

Family Applications (1)

Application Number Title Priority Date Filing Date
IL12033897A IL120338A0 (en) 1997-02-27 1997-02-27 Single step DNA fragments assembly

Country Status (3)

Country Link
AU (2) AU6228798A (en)
IL (1) IL120338A0 (en)
WO (2) WO1998038297A1 (en)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US7018793B1 (en) 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
US6562594B1 (en) 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6825011B1 (en) 1998-12-17 2004-11-30 Yuri Rumantichikov Methods for insertion of nucleic acids into circular vectors
DE19736591A1 (en) * 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Preparing long nucleic acid polymers from linkable oligonucleotides
CA2329122A1 (en) * 1999-03-26 2000-10-05 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
EP1741787A1 (en) * 2000-03-07 2007-01-10 University Of Guelph DNA joining method
CA2317865A1 (en) * 2000-03-07 2001-09-07 University Of Guelph Dna joining method
US7908087B2 (en) 2002-03-18 2011-03-15 Yeda Research And Development Co. Ltd. Methods of identifying latent splice sites
FR2882063B1 (en) * 2005-02-15 2007-06-22 Univ Aix Marseille Ii PROCESS FOR THE PREPARATION OF SYNTHETIC FRAGMENTS OF BICATENE NUCLEIC ACIDS
ATE483802T1 (en) * 2005-08-11 2010-10-15 Synthetic Genomics Inc METHOD FOR IN VITRO RECOMBINATION
CA2618699C (en) 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
EP1968994B1 (en) * 2005-12-06 2013-07-03 Synthetic Genomics, Inc. Synthetic genomes
US20070292954A1 (en) * 2006-04-21 2007-12-20 The Brigham And Women's Hospital, Inc. Generation of recombinant DNA by sequence-and ligation-independent cloning
US8053191B2 (en) * 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
CN107384910A (en) * 2008-02-15 2017-11-24 合成基因组公司 The method of Ligation in vitro and combination assembling nucleic acid molecules
WO2012040628A2 (en) * 2010-09-24 2012-03-29 Massachusetts Institute Of Technology Rapid assemby of multiple arbitrary length dna fragments
EP3705573A1 (en) 2010-11-12 2020-09-09 Gen9, Inc. Methods and devices for nucleic acids synthesis
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
ES2737957T3 (en) 2011-08-26 2020-01-17 Gen9 Inc Compositions and methods for high fidelity nucleic acid assembly
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
AU2013251701A1 (en) 2012-04-24 2014-10-30 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20150191719A1 (en) 2012-06-25 2015-07-09 Gen9, Inc. Methods for Nucleic Acid Assembly and High Throughput Sequencing
CN102787114B (en) * 2012-08-11 2015-06-17 复旦大学 Efficient directional seamless DNA (deoxyribonucleic acid) segment connecting method
KR101417989B1 (en) * 2013-02-08 2014-07-09 주식회사 엔지노믹스 Method for regulating length of overhang of double stranded DNA
WO2016033315A2 (en) 2014-08-27 2016-03-03 New England Biolabs, Inc. Synthon formation
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
CN106995813B (en) * 2017-03-23 2020-06-16 山东大学 New technology for direct cloning of large genome segment and DNA multi-molecule assembly

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE143696T1 (en) * 1989-02-28 1996-10-15 Canon Kk PARTIALLY DOUBLE STRANDED OLIGONUCLEOTIDE AND METHOD FOR FORMING IT
US5580759A (en) * 1994-02-03 1996-12-03 Board Of Regents, The University Of Texas System Construction of recombinant DNA by exonuclease recession

Also Published As

Publication number Publication date
WO1998038297A1 (en) 1998-09-03
AU6228798A (en) 1998-09-18
WO1998038299A1 (en) 1998-09-03
AU6112598A (en) 1998-09-18

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