JP2013531978A5 - - Google Patents
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- Publication number
- JP2013531978A5 JP2013531978A5 JP2013512251A JP2013512251A JP2013531978A5 JP 2013531978 A5 JP2013531978 A5 JP 2013531978A5 JP 2013512251 A JP2013512251 A JP 2013512251A JP 2013512251 A JP2013512251 A JP 2013512251A JP 2013531978 A5 JP2013531978 A5 JP 2013531978A5
- Authority
- JP
- Japan
- Prior art keywords
- pdhk
- nucleic acid
- acid sequence
- cell
- heterologous nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000053067 Pyruvate Dehydrogenase Acetyl-Transferring Kinase Human genes 0.000 claims 47
- 101710159466 [Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial Proteins 0.000 claims 47
- 210000004027 cell Anatomy 0.000 claims 40
- 150000007523 nucleic acids Chemical group 0.000 claims 40
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 39
- 108020004459 Small interfering RNA Proteins 0.000 claims 32
- 239000004055 small Interfering RNA Substances 0.000 claims 32
- 238000000034 method Methods 0.000 claims 29
- 102000003855 L-lactate dehydrogenase Human genes 0.000 claims 26
- 108700023483 L-lactate dehydrogenases Proteins 0.000 claims 26
- 210000004748 cultured cell Anatomy 0.000 claims 24
- 101100243116 Homo sapiens PDK1 gene Proteins 0.000 claims 10
- 101100463123 Homo sapiens PDK3 gene Proteins 0.000 claims 10
- 101001117143 Homo sapiens [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial Proteins 0.000 claims 10
- 102100024148 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial Human genes 0.000 claims 10
- 102100024150 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial Human genes 0.000 claims 10
- 102100034824 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial Human genes 0.000 claims 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims 10
- 238000004519 manufacturing process Methods 0.000 claims 10
- 229920001184 polypeptide Polymers 0.000 claims 8
- 102000004196 processed proteins & peptides Human genes 0.000 claims 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims 8
- 235000014655 lactic acid Nutrition 0.000 claims 5
- 239000004310 lactic acid Substances 0.000 claims 5
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims 3
- 101150041530 ldha gene Proteins 0.000 claims 3
- 101100463125 Homo sapiens PDK4 gene Proteins 0.000 claims 2
- 102100034825 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial Human genes 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 210000004962 mammalian cell Anatomy 0.000 claims 2
- 238000013518 transcription Methods 0.000 claims 2
- 230000035897 transcription Effects 0.000 claims 2
- 101710088194 Dehydrogenase Proteins 0.000 claims 1
- 108091000080 Phosphotransferase Proteins 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000002222 downregulating effect Effects 0.000 claims 1
- 230000030279 gene silencing Effects 0.000 claims 1
- 102000020233 phosphotransferase Human genes 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34972710P | 2010-05-28 | 2010-05-28 | |
| US61/349,727 | 2010-05-28 | ||
| PCT/US2011/038191 WO2011150241A2 (en) | 2010-05-28 | 2011-05-26 | Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016227876A Division JP6325060B2 (ja) | 2010-05-28 | 2016-11-24 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013531978A JP2013531978A (ja) | 2013-08-15 |
| JP2013531978A5 true JP2013531978A5 (enExample) | 2014-07-10 |
| JP6050226B2 JP6050226B2 (ja) | 2016-12-21 |
Family
ID=45004831
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013512251A Active JP6050226B2 (ja) | 2010-05-28 | 2011-05-26 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
| JP2016227876A Active JP6325060B2 (ja) | 2010-05-28 | 2016-11-24 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
| JP2018076234A Active JP6706642B2 (ja) | 2010-05-28 | 2018-04-11 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016227876A Active JP6325060B2 (ja) | 2010-05-28 | 2016-11-24 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
| JP2018076234A Active JP6706642B2 (ja) | 2010-05-28 | 2018-04-11 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
Country Status (15)
| Country | Link |
|---|---|
| US (4) | US9487809B2 (enExample) |
| EP (1) | EP2576580B1 (enExample) |
| JP (3) | JP6050226B2 (enExample) |
| KR (3) | KR101989762B1 (enExample) |
| CN (1) | CN102985437B (enExample) |
| BR (1) | BR112012030197A2 (enExample) |
| CA (1) | CA2800728C (enExample) |
| DK (1) | DK2576580T3 (enExample) |
| ES (1) | ES2599412T3 (enExample) |
| HU (1) | HUE030820T2 (enExample) |
| MX (3) | MX374851B (enExample) |
| PL (1) | PL2576580T3 (enExample) |
| RU (1) | RU2614125C2 (enExample) |
| SI (1) | SI2576580T1 (enExample) |
| WO (1) | WO2011150241A2 (enExample) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2831860B2 (ja) | 1991-03-29 | 1998-12-02 | 株式会社東芝 | 連続ラインの連続式冷却槽温度制御装置 |
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| NZ592097A (en) | 2008-10-20 | 2013-01-25 | Abbott Lab | Viral inactivation during purification of il-12 and il-18 antibodies |
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| CA2800728C (en) | 2010-05-28 | 2020-10-27 | Genentech, Inc. | Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase |
| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| US9505833B2 (en) | 2012-04-20 | 2016-11-29 | Abbvie Inc. | Human antibodies that bind human TNF-alpha and methods of preparing the same |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| WO2013176754A1 (en) | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| SG11201504249XA (en) | 2012-09-02 | 2015-07-30 | Abbvie Inc | Methods to control protein heterogeneity |
| US8921526B2 (en) | 2013-03-14 | 2014-12-30 | Abbvie, Inc. | Mutated anti-TNFα antibodies and methods of their use |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| US9499614B2 (en) | 2013-03-14 | 2016-11-22 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides |
| AR095196A1 (es) | 2013-03-15 | 2015-09-30 | Regeneron Pharma | Medio de cultivo celular libre de suero |
| SMT202100691T1 (it) | 2013-07-11 | 2022-01-10 | Modernatx Inc | Composizioni 5 comprendenti polinucleotidi sintetici che codificano proteine correlate a crispr e sgrna sintetici e metodi d'uso |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| SG10201800772TA (en) * | 2013-10-11 | 2018-03-28 | Regeneron Pharma | Metabolically optimized cell culture |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| WO2015073884A2 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| WO2015142903A2 (en) * | 2014-03-17 | 2015-09-24 | Genentech, Inc. | Method of controlling lactate production with piperdine-dione derivatives |
| EP3666896A1 (en) | 2014-10-10 | 2020-06-17 | Dicerna Pharmaceuticals, Inc. | Therapeutic inhibition of lactate dehydrogenase and agents therefor |
| KR102265924B1 (ko) * | 2014-10-31 | 2021-06-15 | 삼성바이오에피스 주식회사 | Pdk 불활성화에 의한 폴리펩타이드의 생산 |
| WO2016125937A1 (ko) * | 2015-02-06 | 2016-08-11 | 경북대학교 산학협력단 | Pdk2를 이용한 여성 갱년기 질환 예방 또는 치료제의 스크리닝 방법 및 상기 방법으로 스크리닝 된 치료제를 유효성분으로 포함하는 여성 갱년기 질환의 예방 또는 치료용 약학적 조성물 |
| CN105368839A (zh) * | 2015-12-22 | 2016-03-02 | 中国人民解放军第三军医大学 | 一种有效抑制小鼠pdk2表达的rna干扰序列及其应用 |
| EP3452080A4 (en) * | 2016-05-02 | 2019-10-09 | The Regents of the University of California | MAMMALIAN CELLS WITHOUT LACTATE DEHYDROGENASE ACTIVITY |
| IL273875B2 (en) | 2017-10-13 | 2025-07-01 | Dicerna Pharmaceuticals Inc | Methods and compositions for inhibiting expression of ldha |
| CN107904257B (zh) * | 2017-11-22 | 2021-05-04 | 上海药明生物技术有限公司 | 一种增加cho细胞群蛋白表达量的方法 |
| JP2021505662A (ja) | 2017-12-04 | 2021-02-18 | ザ ジェネラル ホスピタル コーポレイション | 疾患を軽減するための細胞外酸化還元酵素系 |
| EP3775184B1 (en) * | 2018-03-29 | 2025-12-10 | F. Hoffmann-La Roche AG | Modulating lactogenic activity in mammalian cells |
| AU2020306672B2 (en) * | 2019-06-26 | 2023-08-24 | F. Hoffmann-La Roche Ag | Mammalian cell lines with SIRT-1 gene knockout |
| US12297451B1 (en) | 2019-10-25 | 2025-05-13 | Regeneron Pharmaceuticals, Inc. | Cell culture medium |
| AU2021271317A1 (en) | 2020-05-15 | 2023-01-19 | Crispr Therapeutics Ag | Messenger RNA encoding Cas9 for use in genome-editing systems |
| CN115948404B (zh) * | 2023-02-01 | 2024-06-04 | 重庆医科大学 | 一种抑制环状RNA circPDHK1的siRNA及其用途 |
| EP4574966A1 (en) * | 2023-12-18 | 2025-06-25 | Good Meat, Inc. | Engineered cell lines for the production of cultivated meat |
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-
2011
- 2011-05-26 CA CA2800728A patent/CA2800728C/en active Active
- 2011-05-26 KR KR1020187016873A patent/KR101989762B1/ko not_active Expired - Fee Related
- 2011-05-26 DK DK11787435.4T patent/DK2576580T3/en active
- 2011-05-26 CN CN201180026517.2A patent/CN102985437B/zh active Active
- 2011-05-26 HU HUE11787435A patent/HUE030820T2/en unknown
- 2011-05-26 BR BR112012030197A patent/BR112012030197A2/pt not_active Application Discontinuation
- 2011-05-26 MX MX2016014767A patent/MX374851B/es unknown
- 2011-05-26 ES ES11787435.4T patent/ES2599412T3/es active Active
- 2011-05-26 WO PCT/US2011/038191 patent/WO2011150241A2/en not_active Ceased
- 2011-05-26 PL PL11787435T patent/PL2576580T3/pl unknown
- 2011-05-26 MX MX2012013579A patent/MX343604B/es active IP Right Grant
- 2011-05-26 SI SI201131017A patent/SI2576580T1/sl unknown
- 2011-05-26 EP EP11787435.4A patent/EP2576580B1/en active Active
- 2011-05-26 RU RU2012157307A patent/RU2614125C2/ru active
- 2011-05-26 KR KR1020127033948A patent/KR101869987B1/ko not_active Expired - Fee Related
- 2011-05-26 JP JP2013512251A patent/JP6050226B2/ja active Active
- 2011-05-26 KR KR1020197016381A patent/KR20190067277A/ko not_active Withdrawn
-
2012
- 2012-11-22 MX MX2020009460A patent/MX2020009460A/es unknown
- 2012-11-28 US US13/688,075 patent/US9487809B2/en active Active
-
2016
- 2016-09-16 US US15/268,424 patent/US10011856B2/en active Active
- 2016-11-24 JP JP2016227876A patent/JP6325060B2/ja active Active
-
2018
- 2018-04-11 JP JP2018076234A patent/JP6706642B2/ja active Active
- 2018-05-31 US US15/994,950 patent/US10704071B2/en active Active
-
2020
- 2020-05-29 US US16/888,344 patent/US11046987B2/en active Active
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