JP2013515249A - 物体の２次元または３次元の位置調整のための高解像度顕微鏡および方法 - Google Patents

物体の２次元または３次元の位置調整のための高解像度顕微鏡および方法 Download PDF

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JP2013515249A
JP2013515249A JP2012545135A JP2012545135A JP2013515249A JP 2013515249 A JP2013515249 A JP 2013515249A JP 2012545135 A JP2012545135 A JP 2012545135A JP 2012545135 A JP2012545135 A JP 2012545135A JP 2013515249 A JP2013515249 A JP 2013515249A
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JP2013515249A5 (enExample
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カルクブレナー、トーマス
ヴォレシェンスキー、ラルフ
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Carl Zeiss Microscopy GmbH
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361—Optical details, e.g. image relay to the camera or image sensor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Optics & Photonics (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
JP2012545135A 2009-12-22 2010-12-14 物体の２次元または３次元の位置調整のための高解像度顕微鏡および方法 Pending JP2013515249A (ja)

Applications Claiming Priority (3)

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DE102009060793.5 2009-12-22
DE102009060793A DE102009060793A1 (de) 2009-12-22 2009-12-22 Hochauflösendes Mikroskop und Verfahren zur zwei- oder dreidimensionalen Positionsbestimmung von Objekten
PCT/EP2010/007595 WO2011085766A1 (de) 2009-12-22 2010-12-14 Hochauflösendes mikroskop und verfahren zur zwei- oder dreidimensionalen positionsbestimmung von objekten

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JP2013515249A5 JP2013515249A5 (enExample) 2013-08-08

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JP2012545135A Pending JP2013515249A (ja) 2009-12-22 2010-12-14 物体の２次元または３次元の位置調整のための高解像度顕微鏡および方法
JP2015121233A Active JP6018263B2 (ja) 2009-12-22 2015-06-16 物体の２次元または３次元の位置調整のための高解像度顕微鏡および方法
JP2016191256A Active JP6282706B2 (ja) 2009-12-22 2016-09-29 物体の２次元または３次元の位置調整のための高解像度顕微鏡および方法

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JP2016191256A Active JP6282706B2 (ja) 2009-12-22 2016-09-29 物体の２次元または３次元の位置調整のための高解像度顕微鏡および方法

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US (1) US9234846B2 (enExample)
EP (1) EP2516993B1 (enExample)
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DE (1) DE102009060793A1 (enExample)
WO (1) WO2011085766A1 (enExample)

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JP2013057938A (ja) * 2011-09-02 2013-03-28 Leica Microsystems Cms Gmbh 点状対象物の３次元位置決め用の顕微鏡装置および顕微鏡法
JP2014224813A (ja) * 2013-05-14 2014-12-04 カール ツァイス マイクロスコピー ゲーエムベーハーＣａｒｌ Ｚｅｉｓｓ Ｍｉｃｒｏｓｃｏｐｙ Ｇｍｂｈ ３ｄ高解像度局在顕微鏡法のための方法
JP2014224814A (ja) * 2013-05-14 2014-12-04 カール ツァイス マイクロスコピー ゲーエムベーハーＣａｒｌ Ｚｅｉｓｓ Ｍｉｃｒｏｓｃｏｐｙ Ｇｍｂｈ ３ｄ高解像度局在顕微鏡法のための方法
JP2015505620A (ja) * 2012-01-24 2015-02-23 カール ツァイス マイクロスコピー ゲーエムベーハーＣａｒｌ Ｚｅｉｓｓ Ｍｉｃｒｏｓｃｏｐｙ Ｇｍｂｈ 高分解能３ｄ蛍光顕微鏡法のための顕微鏡および方法
JP2015531072A (ja) * 2012-08-23 2015-10-29 アイシス イノヴェイション リミテッド 誘導放出抑制顕微鏡検査
JP2016515718A (ja) * 2013-03-22 2016-05-30 ライカ ミクロジュステムス ツェーエムエス ゲーエムベーハー 点状物体の局在化のための光学顕微鏡法
JP2016534395A (ja) * 2013-08-14 2016-11-04 カール ツァイス マイクロスコピー ゲーエムベーハーＣａｒｌ Ｚｅｉｓｓ Ｍｉｃｒｏｓｃｏｐｙ Ｇｍｂｈ 高解像度の３ｄ蛍光顕微鏡検査
KR20170010590A (ko) * 2015-07-20 2017-02-01 주식회사 토모큐브 파면 제어기를 활용한 초고속 고정밀 3차원 굴절률 측정 방법 및 장치
JP2017506370A (ja) * 2014-02-12 2017-03-02 カール ツァイス マイクロスコピー ゲーエムベーハーＣａｒｌ Ｚｅｉｓｓ Ｍｉｃｒｏｓｃｏｐｙ Ｇｍｂｈ 複数焦点走査蛍光顕微鏡
WO2017104662A1 (ja) * 2015-12-18 2017-06-22 株式会社堀場製作所 粒子分析装置及び粒子分析方法
WO2018128146A1 (ja) * 2017-01-05 2018-07-12 東レエンジニアリング株式会社 分光測定方法および分光測定装置
JPWO2017090211A1 (ja) * 2015-11-27 2018-09-06 株式会社ニコン 顕微鏡装置
JP2019086529A (ja) * 2017-11-01 2019-06-06 株式会社ニコン 顕微鏡、照明装置、及び観察方法

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DE102013208415B4 (de) * 2013-05-07 2023-12-28 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren für die 3D-hochauflösende Lokalisierungsmikroskopie
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WO2015089506A2 (en) 2013-12-15 2015-06-18 President And Fellows Of Harvard College Methods and compositions relating to optical super-resolution patterning
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JP6300658B2 (ja) * 2014-06-19 2018-03-28 オリンパス株式会社 標本観察装置
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DE102015016353A1 (de) 2015-12-16 2017-06-22 Horst Wochnowski PALM-Verfahren mit mehreren sich nur marginal voneinander unterscheidenden Fluorophoren
CN106546513B (zh) * 2016-11-02 2019-04-23 中国人民解放军理工大学 一种基于正交双视场的三维降水粒子测量与重构装置及方法
CN107167929B (zh) * 2017-06-12 2019-06-25 华南师范大学 基于dmd的双模式光学超分辨显微成像装置及方法
JP2019066706A (ja) 2017-10-02 2019-04-25 ソニー株式会社 蛍光顕微鏡装置及び蛍光顕微鏡システム
WO2019135069A1 (en) * 2018-01-02 2019-07-11 King's College London Method and system for localisation microscopy
JP7380569B2 (ja) * 2018-08-09 2023-11-15 ソニーグループ株式会社 光学顕微鏡装置及び光学顕微鏡システム
CN109407295B (zh) * 2018-12-18 2020-07-24 中国科学院深圳先进技术研究院 一种基于dmd可多色激发的结构光显微系统及多色激发方法
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CN111650739B (zh) * 2020-05-21 2022-06-03 中国科学院苏州生物医学工程技术研究所 基于dmd的单帧曝光快速三维荧光成像系统及方法
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JP2017506370A (ja) * 2014-02-12 2017-03-02 カール ツァイス マイクロスコピー ゲーエムベーハーＣａｒｌ Ｚｅｉｓｓ Ｍｉｃｒｏｓｃｏｐｙ Ｇｍｂｈ 複数焦点走査蛍光顕微鏡
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JPWO2017090211A1 (ja) * 2015-11-27 2018-09-06 株式会社ニコン 顕微鏡装置
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