JP2013503630A5 - - Google Patents

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JP2013503630A5
JP2013503630A5 JP2012527909A JP2012527909A JP2013503630A5 JP 2013503630 A5 JP2013503630 A5 JP 2013503630A5 JP 2012527909 A JP2012527909 A JP 2012527909A JP 2012527909 A JP2012527909 A JP 2012527909A JP 2013503630 A5 JP2013503630 A5 JP 2013503630A5
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droplet
droplets
composite
sample
reagent
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JP2013503630A (ja
JP6155418B2 (ja
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JP2012527909A 2009-09-02 2010-08-24 多重エマルジョンの合体による、流体を混合するためのシステム Active JP6155418B2 (ja)

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Application Number Priority Date Filing Date Title
US27586009P 2009-09-02 2009-09-02
US61/275,860 2009-09-02
PCT/US2010/046519 WO2011028539A1 (en) 2009-09-02 2010-08-24 System for mixing fluids by coalescence of multiple emulsions

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JP2013503630A JP2013503630A (ja) 2013-02-04
JP2013503630A5 true JP2013503630A5 (enExample) 2013-10-10
JP6155418B2 JP6155418B2 (ja) 2017-07-05

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US (3) US9194861B2 (enExample)
EP (2) EP2940153B1 (enExample)
JP (1) JP6155418B2 (enExample)
CA (3) CA2767056C (enExample)
WO (1) WO2011028539A1 (enExample)

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Publication number Priority date Publication date Assignee Title
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP2363205A3 (en) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530168B1 (en) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Microfluidic Devices
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US8633015B2 (en) * 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
CA2738578C (en) 2008-09-23 2021-05-04 Quantalife, Inc. Droplet-based assay system
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
WO2011028764A2 (en) 2009-09-02 2011-03-10 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
CA2767056C (en) * 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP5934657B2 (ja) 2010-02-12 2016-06-15 レインダンス テクノロジーズ, インコーポレイテッド デジタル検体分析
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
EP2556170A4 (en) 2010-03-25 2014-01-01 Quantalife Inc TRAPPING TRANSPORT AND DETECTION SYSTEM
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
SG190074A1 (en) 2010-11-01 2013-06-28 Bio Rad Laboratories System for forming emulsions
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
EP2694213B1 (en) * 2011-04-08 2020-05-06 Stokes Bio Limited Biological detection system
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
DE202012013668U1 (de) 2011-06-02 2019-04-18 Raindance Technologies, Inc. Enzymquantifizierung
US20140220350A1 (en) 2011-07-06 2014-08-07 President And Fellows Of Harvard College Multiple emulsions and techniques for the formation of multiple emulsions
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
WO2013029159A1 (en) * 2011-08-30 2013-03-07 The Royal Institution For The Advancement Of Learning / Mcgill University Method and system for pre-programmed self-power microfluidic circuits
CN106423314B (zh) * 2011-09-28 2021-03-02 哈佛学院院长等 用于液滴产生和/或流体操纵的系统和方法
WO2013101741A1 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
WO2013192351A1 (en) 2012-06-20 2013-12-27 Bio-Rad Laboratories, Inc. Stabilized droplets for calibration and testing
WO2014018562A1 (en) * 2012-07-23 2014-01-30 Bio-Rad Laboratories, Inc. Droplet generation system with features for sample positioning
GB2539836B (en) 2012-08-13 2017-03-29 Univ California Methods for detecting target nucleic acids in sample lysate droplets
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP6367196B2 (ja) 2012-08-14 2018-08-01 テンエックス・ジェノミクス・インコーポレイテッド マイクロカプセル組成物および方法
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9422602B2 (en) 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
EP2895591A4 (en) 2012-09-12 2016-10-12 Gnubio Inc INTEGRATED MICROFLUIDIC SYSTEM, METHOD AND KIT FOR PERFORMING TESTS
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093714A1 (en) 2012-12-14 2014-06-19 Bio-Rad Laboratories, Inc. Methods and compositions for using oils for analysis and detection of molecules
FR3000688B1 (fr) * 2013-01-08 2016-09-30 Centre Nat De La Rech Scient - Cnrs - Procede pour activer une reaction chimique, melange activable par ce procede et dispostiif pour la mise en oeuvre de ce procede
KR102190198B1 (ko) 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
WO2014145555A1 (en) * 2013-03-15 2014-09-18 Lariat Biosciences, Inc. Microfluidic methods for manipulating dna
EP3004391B1 (en) * 2013-05-29 2019-03-27 Bio-Rad Laboratories, Inc. Methods for sequencing in emulsion based microfluidics
WO2015019522A1 (ja) * 2013-08-08 2015-02-12 パナソニック株式会社 核酸増幅デバイス、核酸増幅装置及び核酸増幅方法
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
JP6700173B2 (ja) * 2013-09-24 2020-05-27 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア ターゲット検出方法及びシステム
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
GB201321433D0 (en) * 2013-12-04 2014-01-15 Isis Innovation Controling fluid micro compartments
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CA2943624A1 (en) * 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
WO2015160919A1 (en) * 2014-04-16 2015-10-22 President And Fellows Of Harvard College Systems and methods for producing droplet emulsions with relatively thin shells
WO2015170268A1 (en) * 2014-05-09 2015-11-12 Dh Technologies Development Pte. Ltd. Fluid transfer from digital microfluidic device
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2015200869A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
JP2017526046A (ja) 2014-06-26 2017-09-07 10エックス ゲノミクス,インコーポレイテッド 核酸配列アセンブルのプロセス及びシステム
WO2015200717A2 (en) 2014-06-27 2015-12-30 The Regents Of The University Of California Pcr-activated sorting (pas)
US20170268031A1 (en) * 2014-07-30 2017-09-21 The Regents Of The University Of California Methods for bacteriophage detection
WO2016065056A1 (en) 2014-10-22 2016-04-28 The Regents Of The University Of California High definition microdroplet printer
WO2016069939A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN107427808B (zh) 2015-01-12 2020-10-23 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
WO2016115273A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
CA2974299A1 (en) * 2015-02-04 2016-08-11 The Regents Of The University Of California Multiple-emulsion nucleic acid amplification
AU2016215304B2 (en) 2015-02-04 2022-01-27 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
WO2016127073A1 (en) * 2015-02-06 2016-08-11 The Regents Of The University Of Michigan Controlled coalescence of gas phase segmented droplets
CN107208156B (zh) 2015-02-09 2021-10-08 10X基因组学有限公司 用于使用变异识别数据来确定结构变异和定相的系统和方法
CN107532202A (zh) 2015-02-24 2018-01-02 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
CN105936930A (zh) * 2015-03-04 2016-09-14 松下知识产权经营株式会社 Dna检测方法和dna检测装置
CN105969655A (zh) * 2015-03-10 2016-09-28 松下知识产权经营株式会社 多个核酸靶标的分析方法
FR3038530B1 (fr) * 2015-07-07 2017-08-18 Ecole Superieure Physique & Chimie Ind Ville De Paris Procede de recuperation de gouttes et systeme de recuperation de gouttes associe
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017189003A1 (en) 2016-04-29 2017-11-02 Hewlett-Packard Development Company, L.P. Printing with an emulsion
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
EP3269444A1 (en) 2016-07-14 2018-01-17 Base4 Innovation Ltd Method of identifying droplets in a stack and an associated sequencer
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
CN110462053A (zh) 2016-12-21 2019-11-15 加利福尼亚大学董事会 使用基于水凝胶的液滴进行单细胞基因组测序
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018115978A2 (en) * 2016-12-23 2018-06-28 University Of Limerick Water-in-oil-in water emulsions for analysis of biological and chemical samples
FR3061665B1 (fr) * 2017-01-09 2019-05-31 Millidrop Instruments Sas Systeme de recuperation de gouttes et procede associe
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US10408852B2 (en) * 2017-04-26 2019-09-10 Lawrence Livermore National Security, Llc Automated control of microfluidic devices based on machine learning
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP4215616B1 (en) 2017-05-18 2024-09-04 10X Genomics, Inc. Methods and systems for sorting droplets and beads
CN110870018B (zh) 2017-05-19 2024-11-22 10X基因组学有限公司 用于分析数据集的系统和方法
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10357771B2 (en) 2017-08-22 2019-07-23 10X Genomics, Inc. Method of producing emulsions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN111479631B (zh) 2017-10-27 2022-02-22 10X基因组学有限公司 用于样品制备和分析的方法和系统
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
US11779923B2 (en) * 2017-12-06 2023-10-10 Samplix Aps Microfluidic device and a method for provision of double emulsion droplets
CN111699388B (zh) 2017-12-12 2024-08-02 10X基因组学有限公司 用于单细胞处理的系统和方法
CN111712579B (zh) 2017-12-22 2024-10-15 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
CA3095588A1 (en) 2018-04-02 2019-10-10 Dropworks, Inc. Systems and methods for serial flow emulsion processes
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
FR3082440B1 (fr) * 2018-06-14 2020-12-11 Paris Sciences Lettres Quartier Latin Methode de transfert de matiere dans un dispositif microfluidique ou millifluidique
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
SG11202101164TA (en) 2018-08-03 2021-03-30 10X Genomics Inc Methods and systems to minimize barcode exchange
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
EP3613498A1 (en) 2018-08-24 2020-02-26 Université de Liège Microfluidic module for co-encapsulation in droplets
US10913067B2 (en) * 2018-10-01 2021-02-09 Sharp Life Science (Eu) Limited Barrier droplet configurations against migration between droplets on AM-EWOD devices
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
WO2020167862A1 (en) * 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
EP3924505B1 (en) 2019-02-12 2025-12-17 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
EP3930900A1 (en) 2019-02-28 2022-01-05 10X Genomics, Inc. Devices, systems, and methods for increasing droplet formation efficiency
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
EP3947722A1 (en) 2019-03-27 2022-02-09 10X Genomics, Inc. Systems and methods for processing rna from cells
EP3973074A4 (en) 2019-05-22 2023-09-06 Mission Bio, Inc. METHOD AND DEVICE FOR SIMULTANEOUS TARGETED SEQUENCING OF DNA, RNA AND PROTEIN
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
US11919002B2 (en) 2019-08-20 2024-03-05 10X Genomics, Inc. Devices and methods for generating and recovering droplets
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
US12421558B2 (en) 2020-02-13 2025-09-23 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and DNA chromatin accessibility
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
CN120158497A (zh) 2021-02-23 2025-06-17 10X基因组学有限公司 基于探针的核酸和蛋白质分析
KR102680093B1 (ko) * 2021-07-21 2024-06-28 동국대학교 산학협력단 화장품 제조 장치
EP4409289A1 (en) * 2021-10-01 2024-08-07 PNP Research LLC Enhanced binding of target-specific nanoparticle markers
US20250101414A1 (en) * 2022-01-10 2025-03-27 Cz Biohub Sf, Llc Particle-templated emulsification in well plates
GB2616677A (en) 2022-03-18 2023-09-20 Sphere Fluidics Ltd Droplet formation system and method
EP4638006A1 (en) 2022-12-19 2025-10-29 Anchorline Biolabs, Inc. Capillary partitioning microfluidics
IL327356A (en) 2023-10-18 2026-05-01 Amgen Inc Quantitative assessment of mRNA transcript levels using DDPCR for initial pool characterization and evaluation of single-cell clones in the cell line development process

Family Cites Families (270)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3575220A (en) 1968-08-12 1971-04-20 Scientific Industries Apparatus for dispensing liquid sample
BR7500831A (pt) 1974-02-11 1975-12-02 Fmc Corp Aparelho de separacao de bolhas de gas corpo de transmissao de gas para uso no aparelho processo para fabricacao do dito corpo e para difusao de bolhas finas de gas em um corpo de liquido
US4051025A (en) 1976-09-29 1977-09-27 The United States Of America As Represented By The Department Of Health, Education And Welfare Preparative countercurrent chromatography with a slowly rotating helical tube array
US4201691A (en) 1978-01-16 1980-05-06 Exxon Research & Engineering Co. Liquid membrane generator
US4348111A (en) 1978-12-07 1982-09-07 The English Electric Company Limited Optical particle analyzers
US4283262A (en) 1980-07-01 1981-08-11 Instrumentation Laboratory Inc. Analysis system
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
US4399219A (en) 1981-01-29 1983-08-16 Massachusetts Institute Of Technology Process for isolating microbiologically active material
US4636075A (en) 1984-08-22 1987-01-13 Particle Measuring Systems, Inc. Particle measurement utilizing orthogonally polarized components of a laser beam
US4948961A (en) 1985-08-05 1990-08-14 Biotrack, Inc. Capillary flow device
US5225332A (en) 1988-04-22 1993-07-06 Massachusetts Institute Of Technology Process for manipulation of non-aqueous surrounded microdroplets
US5055390A (en) 1988-04-22 1991-10-08 Massachusetts Institute Of Technology Process for chemical manipulation of non-aqueous surrounded microdroplets
JPH082416B2 (ja) 1988-09-29 1996-01-17 宮崎県 エマルションの製造方法
AU639008B2 (en) 1989-06-22 1993-07-15 Alliance Pharmaceutical Corporation Fluorine and phosphorous-containing amphiphilic molecules with surfactant properties
GB8917963D0 (en) 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
GB9016163D0 (en) 1990-07-24 1990-09-05 Cemu Bioteknik Chemical method
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
ES2091976T3 (es) 1991-06-20 1996-11-16 Hoffmann La Roche Metodos perfeccionados para la amplificacion del acido nucleico.
US5422277A (en) 1992-03-27 1995-06-06 Ortho Diagnostic Systems Inc. Cell fixative composition and method of staining cells without destroying the cell surface
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
DE69429038T2 (de) 1993-07-28 2002-03-21 Pe Corporation (Ny), Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
US5538667A (en) 1993-10-28 1996-07-23 Whitehill Oral Technologies, Inc. Ultramulsions
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CA2159830C (en) 1994-04-29 2001-07-03 Applied Biosystems, Llc System for real time detection of nucleic acid amplification products
US5972716A (en) 1994-04-29 1999-10-26 The Perkin-Elmer Corporation Fluorescence monitoring device with textured optical tube and method for reducing background fluorescence
EP0695941B1 (en) 1994-06-08 2002-07-31 Affymetrix, Inc. Method and apparatus for packaging a chip
US5555191A (en) 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
FI100859B (sv) 1994-10-14 1998-03-13 Bjarne Holmbom Förfarande och anordning för att utföra on-line flödesextraktion av ex traherbara komponenter i vätskor
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
FR2733766B1 (fr) 1995-05-03 1997-06-06 Rhone Poulenc Rorer Sa Methode de diagnostic de la schizophrenie
US20020022261A1 (en) 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US6057149A (en) 1995-09-15 2000-05-02 The University Of Michigan Microscale devices and reactions in microscale devices
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US6562605B1 (en) 1995-11-13 2003-05-13 Genencor International, Inc. Extraction of water soluble biomaterials from fluids using a carbon dioxide/surfactant mixture
US5736314A (en) 1995-11-16 1998-04-07 Microfab Technologies, Inc. Inline thermo-cycler
EP0889751B1 (en) 1996-04-03 1999-09-08 The Perkin-Elmer Corporation Device and method for multiple analyte detection
CA2258489C (en) 1996-06-28 2004-01-27 Caliper Technologies Corporation High-throughput screening assay systems in microscale fluidic devices
AU726987B2 (en) 1996-06-28 2000-11-30 Caliper Life Sciences, Inc. Electropipettor and compensation means for electrophoretic bias
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6558916B2 (en) 1996-08-02 2003-05-06 Axiom Biotechnologies, Inc. Cell flow apparatus and method for real-time measurements of patient cellular responses
GB9621357D0 (en) 1996-10-12 1996-12-04 Central Research Lab Ltd Heating apparatus
US7268179B2 (en) 1997-02-03 2007-09-11 Cytonix Corporation Hydrophobic coating compositions, articles coated with said compositions, and processes for manufacturing same
EP1498494A3 (en) 1997-04-01 2007-06-20 Solexa Ltd. Method of nucleic acid sequencing
EP1591541B1 (en) 1997-04-01 2012-02-15 Illumina Cambridge Limited Method of nucleic acid sequencing
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
JP2002503336A (ja) 1997-05-16 2002-01-29 アルバータ リサーチ カウンシル 微量流通システムおよびその使用方法
US6664044B1 (en) 1997-06-19 2003-12-16 Toyota Jidosha Kabushiki Kaisha Method for conducting PCR protected from evaporation
US5912945A (en) 1997-06-23 1999-06-15 Regents Of The University Of California X-ray compass for determining device orientation
ES2283936T3 (es) 1997-07-07 2007-11-01 Medical Research Council Procedimiento de clasificacion in vitro.
US5980936A (en) 1997-08-07 1999-11-09 Alliance Pharmaceutical Corp. Multiple emulsions comprising a hydrophobic continuous phase
EP1538206B1 (en) 1997-09-16 2010-03-24 Centocor, Inc. Method for the complete chemical synthesis and assembly of genes and genomes
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US6833242B2 (en) 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US6074725A (en) 1997-12-10 2000-06-13 Caliper Technologies Corp. Fabrication of microfluidic circuits by printing techniques
EP1645222B1 (en) 1998-03-04 2014-01-22 Visx, Incorporated Ophthalmologic surgery system
US6175669B1 (en) 1998-03-30 2001-01-16 The Regents Of The Universtiy Of California Optical coherence domain reflectometry guidewire
JP3081880B2 (ja) 1998-03-30 2000-08-28 農林水産省食品総合研究所長 マイクロスフィアの連続製造装置
US6384915B1 (en) 1998-03-30 2002-05-07 The Regents Of The University Of California Catheter guided by optical coherence domain reflectometry
JP3012608B1 (ja) 1998-09-17 2000-02-28 農林水産省食品総合研究所長 マイクロチャネル装置及び同装置を用いたエマルションの製造方法
US6146103A (en) 1998-10-09 2000-11-14 The Regents Of The University Of California Micromachined magnetohydrodynamic actuators and sensors
US6637463B1 (en) 1998-10-13 2003-10-28 Biomicro Systems, Inc. Multi-channel microfluidic system design with balanced fluid flow distribution
US6176609B1 (en) 1998-10-13 2001-01-23 V & P Scientific, Inc. Magnetic tumble stirring method, devices and machines for mixing in vessels
US6086740A (en) 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US6261431B1 (en) 1998-12-28 2001-07-17 Affymetrix, Inc. Process for microfabrication of an integrated PCR-CE device and products produced by the same
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6522407B2 (en) 1999-01-22 2003-02-18 The Regents Of The University Of California Optical detection dental disease using polarized light
US6326083B1 (en) 1999-03-08 2001-12-04 Calipher Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
DE60029092T2 (de) 1999-04-09 2007-02-01 Exact Sciences Corp., Marlborough Verfahren zur detektion von nukleinsäuren, welche auf krebs hinweisen
US6357907B1 (en) 1999-06-15 2002-03-19 V & P Scientific, Inc. Magnetic levitation stirring devices and machines for mixing in vessels
AU782726B2 (en) 1999-07-28 2005-08-25 Commissariat A L'energie Atomique Integration of biochemical protocols in a continuous flow microfluidic device
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
DE19947495C2 (de) 1999-10-01 2003-05-28 Agilent Technologies Inc Mikrofluidischer Mikrochip
DE60043896D1 (de) 1999-12-07 2010-04-08 Exact Sciences Corp Verfahren zum nachweis von lungenneoplasmen in fäkalen proben
US6620625B2 (en) 2000-01-06 2003-09-16 Caliper Technologies Corp. Ultra high throughput sampling and analysis systems and methods
JP2003522963A (ja) 2000-02-18 2003-07-29 アクララ バイオサイエンシーズ, インコーポレイテッド 複数部位反応デバイスおよび方法
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
JP3282619B2 (ja) 2000-03-22 2002-05-20 住友電装株式会社 ワイヤーハーネスの屈曲試験方法及び電線の屈曲試験装置
US20010048900A1 (en) 2000-05-24 2001-12-06 Bardell Ronald L. Jet vortex mixer
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6466713B2 (en) 2000-08-18 2002-10-15 The Regents Of The University Of California Optical fiber head for providing lateral viewing
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
WO2002022878A1 (en) 2000-09-14 2002-03-21 Caliper Technologies Corp. Microfluidic devices and methods for performing temperature mediated reactions
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
KR100649342B1 (ko) 2000-10-30 2006-11-27 시쿼넘, 인코포레이티드 기판 상으로 서브마이크로리터 볼륨들을 전달하기 위한 방법 및 장치
KR100435921B1 (ko) * 2000-12-29 2004-06-12 주식회사 태평양 동수역학적 이중 안정화에 의한 안정한 수-유-수 다중에멀젼 시스템 및 이의 제조방법
DK1385950T3 (da) 2001-01-19 2008-11-03 Centocor Inc Computerstyret samling af et polynucleotid, som koder for et målpolypeptid
US7567596B2 (en) 2001-01-30 2009-07-28 Board Of Trustees Of Michigan State University Control system and apparatus for use with ultra-fast laser
DE60211857T2 (de) 2001-02-23 2006-12-21 Japan Science And Technology Agency, Kawaguchi Verfahren zur herstellung einer emulsion und vorrichtung dafür
WO2002069016A2 (en) 2001-02-28 2002-09-06 Lightwave Microsystems Corporation Microfluid control for waveguide optical switches, variable attenuators, and other optical devices
US7192557B2 (en) 2001-03-28 2007-03-20 Handylab, Inc. Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US6575188B2 (en) 2001-07-26 2003-06-10 Handylab, Inc. Methods and systems for fluid control in microfluidic devices
US7270786B2 (en) 2001-03-28 2007-09-18 Handylab, Inc. Methods and systems for processing microfluidic samples of particle containing fluids
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn NUCLEIC ACID AMPLIFICATION USING MICROFLUID DEVICES
US6793723B2 (en) 2001-05-10 2004-09-21 Pitney Bowes Inc. Homogeneous photosensitive optically variable ink compositions for ink jet printing
US20030049659A1 (en) 2001-05-29 2003-03-13 Lapidus Stanley N. Devices and methods for isolating samples into subsamples for analysis
US20030013680A1 (en) 2001-06-12 2003-01-16 Keryx Methods using glycosaminoglycans for the treatment of nephropathy
US6905885B2 (en) 2001-06-12 2005-06-14 The Regents Of The University Of California Portable pathogen detection system
US6808683B2 (en) 2001-09-25 2004-10-26 Cytonome, Inc. Droplet dispensing system
CA2451614C (en) 2001-06-25 2011-01-04 Georgia Tech Research Corporation Dual resonance energy transfer nucleic acid probes
US6797257B2 (en) 2001-06-26 2004-09-28 The Board Of Trustees Of The University Of Illinois Paramagnetic polymerized protein microspheres and methods of preparation thereof
US6573490B2 (en) 2001-06-28 2003-06-03 Valeo Electrical Systems, Inc. Interleaved mosaic imaging rain sensor
US20030032172A1 (en) 2001-07-06 2003-02-13 The Regents Of The University Of California Automated nucleic acid assay system
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US20030027150A1 (en) 2001-08-03 2003-02-06 Katz David A. Method of haplotyping and kit therefor
AUPR707101A0 (en) 2001-08-16 2001-09-06 Corbett Research Pty Ltd Continuous flow thermal device
US7094379B2 (en) 2001-10-24 2006-08-22 Commissariat A L'energie Atomique Device for parallel and synchronous injection for sequential injection of different reagents
KR100442836B1 (ko) 2001-11-10 2004-08-02 삼성전자주식회사 생화학 유체를 온도가 다른 폐쇄된 챔버 구간을 따라 회전이동시키는 폐쇄 유체 회로 시스템
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
WO2003048295A1 (en) 2001-11-30 2003-06-12 Fluidigm Corporation Microfluidic device and methods of using same
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
US20030170698A1 (en) 2002-01-04 2003-09-11 Peter Gascoyne Droplet-based microfluidic oligonucleotide synthesis engine
US20030180765A1 (en) 2002-02-01 2003-09-25 The Johns Hopkins University Digital amplification for detection of mismatch repair deficient tumor cells
DE60303504T2 (de) 2002-02-22 2006-10-12 Biodot, Inc., Irvine Verfahren und vorrichtung zur kontaktlosen tröpfchenabgabe unterhalb einer fluidischen oberfläche
EP1488006B1 (en) 2002-03-20 2008-05-28 InnovativeBio.Biz Microcapsules with controlable permeability encapsulating a nucleic acid amplification reaction mixture and their use as reaction compartments for parallels reactions
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US7993266B2 (en) 2002-04-26 2011-08-09 Lawrence Livermore National Security, Llc Early detection of contagious diseases
US7129091B2 (en) 2002-05-09 2006-10-31 University Of Chicago Device and method for pressure-driven plug transport and reaction
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US6794671B2 (en) 2002-07-17 2004-09-21 Particle Sizing Systems, Inc. Sensors and methods for high-sensitivity optical particle counting and sizing
US7188731B2 (en) 2002-08-26 2007-03-13 The Regents Of The University Of California Variable flexure-based fluid filter
US20040038385A1 (en) 2002-08-26 2004-02-26 Langlois Richard G. System for autonomous monitoring of bioagents
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
WO2004074818A2 (en) 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
US20060094108A1 (en) 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
EP1587940A4 (en) 2002-12-20 2006-06-07 Caliper Life Sciences Inc SINGLE MOLECULAR AMPLIFICATION AND DNA PROOF
US20050042639A1 (en) 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
CA2513889A1 (en) 2003-01-29 2004-08-19 454 Corporation Double ended sequencing
US7595195B2 (en) 2003-02-11 2009-09-29 The Regents Of The University Of California Microfluidic devices for controlled viscous shearing and formation of amphiphilic vesicles
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
DK2959774T3 (da) 2003-03-28 2019-05-13 Inguran Llc Fremgangsmåde til kryokonservering af sædceller
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
DE10315640A1 (de) 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
EP1685282A2 (en) 2003-04-17 2006-08-02 Fluidigm Corporation Crystal growth devices and systems, and methods for using same
US7629123B2 (en) 2003-07-03 2009-12-08 University Of Medicine And Dentistry Of New Jersey Compositions and methods for diagnosing autism
EP1649061B1 (en) 2003-07-03 2010-08-25 University Of Medicine And Dentistry Of New Jersey Genes as diagnostic tools for autism
CA2531105C (en) 2003-07-05 2015-03-17 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
EP1654527A4 (en) 2003-08-01 2012-01-04 Ge Healthcare Bio Sciences Ab OPTICAL RESONANCE ANALYSIS UNIT
CN104069784B (zh) 2003-08-27 2017-01-11 哈佛大学 流体物种的电子控制
EP1664077B1 (en) 2003-09-05 2016-04-13 Trustees of Boston University Method for non-invasive prenatal diagnosis
US20050277125A1 (en) 2003-10-27 2005-12-15 Massachusetts Institute Of Technology High-density reaction chambers and methods of use
US7141537B2 (en) 2003-10-30 2006-11-28 3M Innovative Properties Company Mixture of fluorinated polyethers and use thereof as surfactant
US7560033B2 (en) * 2004-10-13 2009-07-14 E.I. Dupont De Nemours And Company Multi-functional oxidizing composition
WO2005055807A2 (en) 2003-12-05 2005-06-23 Beatrice And Samuel A. Seaver Foundation Methods and compositions for autism risk assessment background
US8293471B2 (en) 2004-01-28 2012-10-23 Marshall University Research Corporation Apparatus and method for a continuous rapid thermal cycle system
ES2432040T3 (es) 2004-01-28 2013-11-29 454 Life Sciences Corporation Amplificación de ácido nucleico con emulsión de flujo continuo
CA2555081A1 (en) 2004-02-03 2005-08-18 Postech Foundation High throughput device for performing continuous-flow reactions
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
EP1757357B1 (en) 2004-03-23 2013-04-24 Japan Science and Technology Agency Method and device for producing micro-droplets
US7432106B2 (en) 2004-03-24 2008-10-07 Applied Biosystems Inc. Liquid processing device including gas trap, and system and method
EP3611273A1 (en) 2004-03-31 2020-02-19 The General Hospital Corporation Method to determine responsiveness of cancer to epidermal growth factor receptor targeting treatments
US20050221279A1 (en) 2004-04-05 2005-10-06 The Regents Of The University Of California Method for creating chemical sensors using contact-based microdispensing technology
HU227246B1 (en) 2004-04-30 2010-12-28 Szegedi Biolog Koezpont Use of genes as molecular markers in diagnosis of schizophrenia and diagnostic kit
WO2005110395A1 (en) 2004-05-19 2005-11-24 University Of South Carolina System and device for magnetic drug targeting with magnetic drug carrier particles
US8652851B2 (en) 2004-06-17 2014-02-18 University Of Florida Research Foundation, Inc. Multi-acceptor molecular probes and applications thereof
EP1612279B1 (en) 2004-06-29 2009-03-11 Roche Diagnostics GmbH Association of single nucleotide polymorphisms in PPAR gamma with osteoporosis
WO2006023719A2 (en) 2004-08-20 2006-03-02 Enh Research Institute Identification of snp’s associated with schizophrenia, schizoaffective disorder and bipolar disorder
CN101052468B (zh) 2004-09-09 2012-02-01 居里研究所 采用共线电场的微流控装置
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
WO2006052822A2 (en) 2004-11-05 2006-05-18 Cytolution, Inc. Methods and devices for screening cervical cancer
US20090098044A1 (en) 2004-11-15 2009-04-16 Australian Nuclear Science And Technology Organisation Solid particles from controlled destabilisation of microemulsions
US20060166223A1 (en) 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
US7423751B2 (en) 2005-02-08 2008-09-09 Northrop Grumman Corporation Systems and methods for use in detecting harmful aerosol particles
EP2505591A1 (en) 2005-02-11 2012-10-03 Memorial Sloan-Kettering Cancer Center Methods and compositions for detecting a drug resistant EGFR mutant
US9039273B2 (en) * 2005-03-04 2015-05-26 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
KR20100099333A (ko) 2005-03-05 2010-09-10 주식회사 씨젠 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중 특이성 올리고뉴클레오타이드
US20060269934A1 (en) 2005-03-16 2006-11-30 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
US20070048756A1 (en) 2005-04-18 2007-03-01 Affymetrix, Inc. Methods for whole genome association studies
DE102005037401B4 (de) 2005-08-08 2007-09-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Bildung einer Emulsion in einem fluidischen Mikrosystem
WO2007038670A2 (en) 2005-09-30 2007-04-05 Perlegen Sciences, Inc. Methods and compositions for screening and treatment of disorders of blood glucose regulation
JP4639391B2 (ja) 2005-10-06 2011-02-23 財団法人生産技術研究奨励会 微小液滴の溶合による液滴の形成方法及びその装置
EP2363205A3 (en) * 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
US7976795B2 (en) 2006-01-19 2011-07-12 Rheonix, Inc. Microfluidic systems
WO2007089541A2 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
HUE030215T2 (en) 2006-02-02 2017-04-28 Univ Leland Stanford Junior Non-invasive fetal genetic screening by digital analysis
US20100304446A1 (en) 2006-02-07 2010-12-02 Stokes Bio Limited Devices, systems, and methods for amplifying nucleic acids
EP2298438A1 (en) 2006-02-07 2011-03-23 Stokes Bio Limited A microfluidic droplet queuing network
EP1991357B1 (en) 2006-02-07 2016-09-14 Stokes Bio Limited A microfluidic analysis system
US20070258083A1 (en) 2006-04-11 2007-11-08 Optiscan Biomedical Corporation Noise reduction for analyte detection systems
US8492168B2 (en) 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
WO2009140373A2 (en) 2008-05-13 2009-11-19 Advanced Liquid Logic, Inc. Droplet actuator devices, systems, and methods
US7816121B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet actuation system and method
US7815871B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet microactuator system
EP2530168B1 (en) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Microfluidic Devices
US7608399B2 (en) 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
EP1881079A1 (en) 2006-07-20 2008-01-23 Pangaea Biotech, S.A. Method for the detection of EGFR mutations in blood samples
EP2077912B1 (en) 2006-08-07 2019-03-27 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
US20080044543A1 (en) * 2006-08-17 2008-02-21 Mcclements David J Stabilized emulsions, methods of preparation, and related reduced fat foods
WO2008024114A1 (en) 2006-08-24 2008-02-28 Genizon Biosciences Inc. Genemap of the human genes associated with schizophrenia
EP1912067A1 (en) 2006-10-12 2008-04-16 Eppendorf Array Technologies S.A. Method for quantification of a target compound obtained from a biological sample upon chips
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
WO2008070074A2 (en) 2006-12-04 2008-06-12 Pgxhealth Llc Genetic markers of schizophrenia
EP2140027B1 (en) 2006-12-07 2012-07-18 Novartis AG Non-invasive prenatal genetic screen
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
WO2008089244A2 (en) 2007-01-17 2008-07-24 University Of Rochester Frequency-addressable apparatus and methods for actuation of liquids
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2008109878A2 (en) 2007-03-07 2008-09-12 California Institute Of Technology Testing device
US20100144538A1 (en) 2007-03-08 2010-06-10 Genizon Biosciences Inc. Genemap of the human genes associated with schizophrenia
EP2136786B8 (en) 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
JP5666763B2 (ja) 2007-04-11 2015-02-12 味の素株式会社 油中水型乳化組成物
US8951732B2 (en) 2007-06-22 2015-02-10 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
WO2009011808A1 (en) 2007-07-13 2009-01-22 President And Fellows Of Harvard College Droplet-based selection
CA2694820C (en) 2007-07-30 2015-06-16 F. Hoffman-La Roche Ag Methods of identification using methylation of cpg
US20090053719A1 (en) 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
JP5707132B2 (ja) 2007-09-07 2015-04-22 フルイダイム コーポレイション コピー数変動の決定、方法およびシステム
WO2009049889A1 (en) 2007-10-16 2009-04-23 Roche Diagnostics Gmbh High resolution, high throughput hla genotyping by clonal sequencing
US7807920B2 (en) 2007-10-30 2010-10-05 Opel, Inc. Concentrated solar photovoltaic module
WO2009085246A1 (en) 2007-12-20 2009-07-09 University Of Massachusetts Cross-linked biopolymers, related compositions and methods of use
JP5224801B2 (ja) 2007-12-21 2013-07-03 キヤノン株式会社 核酸増幅装置
US8367370B2 (en) 2008-02-11 2013-02-05 Wheeler Aaron R Droplet-based cell culture and cell assays using digital microfluidics
US20090220434A1 (en) 2008-02-29 2009-09-03 Florida State University Research Foundation Nanoparticles that facilitate imaging of biological tissue and methods of forming the same
US8062903B2 (en) 2008-03-03 2011-11-22 University Of Washington Droplet compartmentalization for chemical separation and on-line sampling
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
US9011777B2 (en) 2008-03-21 2015-04-21 Lawrence Livermore National Security, Llc Monodisperse microdroplet generation and stopping without coalescence
WO2010001419A2 (en) 2008-07-04 2010-01-07 Decode Genetics Ehf Copy number variations predictive of risk of schizophrenia
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US8256931B2 (en) 2008-07-24 2012-09-04 Seward George H Achromatic homogenizer and collimator for LEDs
PL3663411T3 (pl) 2008-08-12 2022-02-07 Stokes Bio Limited Sposoby do cyfrowego pcr
US9180453B2 (en) 2008-08-15 2015-11-10 University Of Washington Method and apparatus for the discretization and manipulation of sample volumes
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20110218123A1 (en) * 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20120171683A1 (en) 2010-03-02 2012-07-05 Ness Kevin D Analysis of fragmented genomic dna in droplets
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US20130084572A1 (en) 2011-09-30 2013-04-04 Quantalife, Inc. Calibrations and controls for droplet-based assays
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US20130017551A1 (en) 2011-07-13 2013-01-17 Bio-Rad Laboratories, Inc. Computation of real-world error using meta-analysis of replicates
US20130059754A1 (en) 2011-09-01 2013-03-07 Bio-Rad Laboratories, Inc. Digital assays with reduced measurement uncertainty
EP2389455A4 (en) 2009-01-26 2012-12-05 Verinata Health Inc METHODS AND COMPOSITIONS FOR IDENTIFYING A FETAL CELL
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
WO2010117461A2 (en) 2009-04-08 2010-10-14 Applied Biosystems, Llc System and method for preparing and using bulk emulsion
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
IN2012DN03431A (enExample) 2009-09-21 2015-10-23 Akonni Biosystems
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
EP2957641B1 (en) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP5934657B2 (ja) 2010-02-12 2016-06-15 レインダンス テクノロジーズ, インコーポレイテッド デジタル検体分析
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
EP2556170A4 (en) 2010-03-25 2014-01-01 Quantalife Inc TRAPPING TRANSPORT AND DETECTION SYSTEM
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
SG190074A1 (en) 2010-11-01 2013-06-28 Bio Rad Laboratories System for forming emulsions
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
EP2673382B1 (en) 2011-02-11 2020-05-06 Bio-Rad Laboratories, Inc. Thermocycling device for nucleic acid amplification and methods of use
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
DE202012013668U1 (de) 2011-06-02 2019-04-18 Raindance Technologies, Inc. Enzymquantifizierung
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay

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