JP2010504758A5 - - Google Patents
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- Publication number
- JP2010504758A5 JP2010504758A5 JP2009530433A JP2009530433A JP2010504758A5 JP 2010504758 A5 JP2010504758 A5 JP 2010504758A5 JP 2009530433 A JP2009530433 A JP 2009530433A JP 2009530433 A JP2009530433 A JP 2009530433A JP 2010504758 A5 JP2010504758 A5 JP 2010504758A5
- Authority
- JP
- Japan
- Prior art keywords
- xylose
- glucose
- recombinant
- ethanol
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 36
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 27
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 18
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 18
- 241000588901 Zymomonas Species 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 11
- 101001059186 Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) Glucose-fructose oxidoreductase Proteins 0.000 claims description 9
- 108010009512 Glucose-fructose oxidoreductase Proteins 0.000 claims description 8
- 238000012239 gene modification Methods 0.000 claims description 8
- 230000005017 genetic modification Effects 0.000 claims description 8
- 235000013617 genetically modified food Nutrition 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 230000004048 modification Effects 0.000 claims description 2
- 238000012986 modification Methods 0.000 claims description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 6
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 6
- 239000000811 xylitol Substances 0.000 description 6
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 6
- 229960002675 xylitol Drugs 0.000 description 6
- 235000010447 xylitol Nutrition 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 239000007836 KH2PO4 Substances 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 4
- 235000019341 magnesium sulphate Nutrition 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 238000011218 seed culture Methods 0.000 description 3
- 108020005544 Antisense RNA Proteins 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 206010010144 Completed suicide Diseases 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 241000588902 Zymomonas mobilis Species 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000011736 potassium bicarbonate Substances 0.000 description 1
- 235000015497 potassium bicarbonate Nutrition 0.000 description 1
- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US84781306P | 2006-09-28 | 2006-09-28 | |
| US60/847,813 | 2006-09-28 | ||
| PCT/US2007/020950 WO2008133638A2 (en) | 2006-09-28 | 2007-09-28 | Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010504758A JP2010504758A (ja) | 2010-02-18 |
| JP2010504758A5 true JP2010504758A5 (https=) | 2010-10-07 |
| JP5346808B2 JP5346808B2 (ja) | 2013-11-20 |
Family
ID=39926224
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009530433A Expired - Fee Related JP5346808B2 (ja) | 2006-09-28 | 2007-09-28 | エタノール産生のためのキシロース資化性ザイモモナス（Ｚｙｍｏｍｏｎａｓ）のキシリトール合成変異体 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7741119B2 (https=) |
| EP (1) | EP2066784B1 (https=) |
| JP (1) | JP5346808B2 (https=) |
| CN (1) | CN101861385B (https=) |
| BR (1) | BRPI0715282A2 (https=) |
| CA (1) | CA2661512C (https=) |
| ES (1) | ES2554805T3 (https=) |
| HU (1) | HUE026720T2 (https=) |
| PL (1) | PL2066784T3 (https=) |
| WO (1) | WO2008133638A2 (https=) |
Families Citing this family (90)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7781191B2 (en) * | 2005-04-12 | 2010-08-24 | E. I. Du Pont De Nemours And Company | Treatment of biomass to obtain a target chemical |
| EP2060632A1 (en) * | 2007-10-29 | 2009-05-20 | Technische Universität Berlin | Method of modifying a yeast cell for the production of ethanol |
| EP2209899B1 (en) * | 2007-10-30 | 2012-04-18 | E. I. du Pont de Nemours and Company | Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate |
| JP5444323B2 (ja) * | 2008-03-27 | 2014-03-19 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | 高発現ザイモモナス（Ｚｙｍｏｍｏｎａｓ）プロモーター |
| JP2011515107A (ja) * | 2008-03-27 | 2011-05-19 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | 改善されたキシロース資化能を有するザイモモナス（Ｚｙｍｏｍｏｎａｓ） |
| WO2010080489A1 (en) * | 2008-12-19 | 2010-07-15 | E. I. Du Pont De Nemours And Company | Ozone treatment of biomass to enhance enzymatic saccharification |
| US8247208B2 (en) * | 2008-12-22 | 2012-08-21 | Alliance For Sustainable Energy Llc | Zymomonas with improved xylose utilization in stress conditions |
| US20110143408A1 (en) | 2009-06-18 | 2011-06-16 | E. I. Du Pont De Nemours And Company | Zymomonas with improved arabinose utilization |
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| RU2012131405A (ru) | 2009-12-23 | 2014-01-27 | ДАНИСКО ЮЭс ИНК. | Способы улучшения эффективности одновременно протекающих реакций осахаривания и ферментации |
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| US9040263B2 (en) | 2010-07-28 | 2015-05-26 | Butamax Advanced Biofuels Llc | Production of alcohol esters and in situ product removal during alcohol fermentation |
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| WO2001083786A2 (en) * | 2000-05-01 | 2001-11-08 | Midwest Research Institute | Stable zymomonas mobilis xylose and arabinose fermenting strains |
| US7374939B1 (en) | 2000-05-01 | 2008-05-20 | Midwest Research Institute | Method of inactivation of an end product of energy metabolism in Zymomonas mobilis |
| US7223575B2 (en) | 2000-05-01 | 2007-05-29 | Midwest Research Institute | Zymomonas pentose-sugar fermenting strains and uses thereof |
| US7354755B2 (en) * | 2000-05-01 | 2008-04-08 | Midwest Research Institute | Stable zymomonas mobilis xylose and arabinose fermenting strains |
| US6566107B1 (en) | 2000-11-01 | 2003-05-20 | Midwest Research Institute | Recombinant Zymomonas mobilis with improved xylose utilization |
| US20040231060A1 (en) | 2003-03-07 | 2004-11-25 | Athenix Corporation | Methods to enhance the activity of lignocellulose-degrading enzymes |
| CN1600850A (zh) | 2003-09-23 | 2005-03-30 | 中国农业科学院生物技术研究所 | 运动发酵单胞菌工程菌及其应用 |
-
2007
- 2007-09-27 US US11/862,566 patent/US7741119B2/en not_active Expired - Fee Related
- 2007-09-28 PL PL07874067T patent/PL2066784T3/pl unknown
- 2007-09-28 ES ES07874067.7T patent/ES2554805T3/es active Active
- 2007-09-28 CA CA2661512A patent/CA2661512C/en not_active Expired - Fee Related
- 2007-09-28 JP JP2009530433A patent/JP5346808B2/ja not_active Expired - Fee Related
- 2007-09-28 WO PCT/US2007/020950 patent/WO2008133638A2/en not_active Ceased
- 2007-09-28 CN CN200780036026XA patent/CN101861385B/zh not_active Expired - Fee Related
- 2007-09-28 BR BRPI0715282-5A patent/BRPI0715282A2/pt active Search and Examination
- 2007-09-28 HU HUE07874067A patent/HUE026720T2/en unknown
- 2007-09-28 EP EP07874067.7A patent/EP2066784B1/en not_active Not-in-force
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