JP2010501181A5 - - Google Patents
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- Publication number
- JP2010501181A5 JP2010501181A5 JP2009525612A JP2009525612A JP2010501181A5 JP 2010501181 A5 JP2010501181 A5 JP 2010501181A5 JP 2009525612 A JP2009525612 A JP 2009525612A JP 2009525612 A JP2009525612 A JP 2009525612A JP 2010501181 A5 JP2010501181 A5 JP 2010501181A5
- Authority
- JP
- Japan
- Prior art keywords
- host organism
- group
- expression construct
- pullulanase
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 19
- 108090000637 alpha-Amylases Proteins 0.000 claims description 13
- 239000002773 nucleotide Substances 0.000 claims description 10
- 125000003729 nucleotide group Chemical group 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 4
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 6
- 241000194108 Bacillus licheniformis Species 0.000 claims 4
- 238000004519 manufacturing process Methods 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims 3
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims 3
- 244000063299 Bacillus subtilis Species 0.000 claims 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims 3
- 241000894006 Bacteria Species 0.000 claims 3
- 241000588724 Escherichia coli Species 0.000 claims 3
- 241000233866 Fungi Species 0.000 claims 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 3
- 238000012258 culturing Methods 0.000 claims 3
- 210000003527 eukaryotic cell Anatomy 0.000 claims 3
- 241000894007 species Species 0.000 claims 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- 241000499912 Trichoderma reesei Species 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 241000223259 Trichoderma Species 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 238000001890 transfection Methods 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 description 9
- 238000004422 calculation algorithm Methods 0.000 description 8
- 125000000539 amino acid group Chemical group 0.000 description 7
- 150000001413 amino acids Chemical class 0.000 description 5
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
- RVCKCEDKBVEEHL-UHFFFAOYSA-N 2,3,4,5,6-pentachlorobenzyl alcohol Chemical compound OCC1=C(Cl)C(Cl)=C(Cl)C(Cl)=C1Cl RVCKCEDKBVEEHL-UHFFFAOYSA-N 0.000 description 1
- 125000000899 L-alpha-glutamyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C([H])([H])C([H])([H])C(O[H])=O 0.000 description 1
- 125000000010 L-asparaginyl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])C(=O)N([H])[H] 0.000 description 1
- 125000000415 L-cysteinyl group Chemical group O=C([*])[C@@](N([H])[H])([H])C([H])([H])S[H] 0.000 description 1
- -1 L-form amino acids Chemical class 0.000 description 1
- 125000002061 L-isoleucyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])[C@](C([H])([H])[H])([H])C(C([H])([H])[H])([H])[H] 0.000 description 1
- 125000001176 L-lysyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C([H])([H])C([H])([H])C([H])([H])C(N([H])[H])([H])[H] 0.000 description 1
- 125000002435 L-phenylalanyl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
- 125000002842 L-seryl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])O[H] 0.000 description 1
- 125000002707 L-tryptophyl group Chemical group [H]C1=C([H])C([H])=C2C(C([C@](N([H])[H])(C(=O)[*])[H])([H])[H])=C([H])N([H])C2=C1[H] 0.000 description 1
- 125000003580 L-valyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(C([H])([H])[H])(C([H])([H])[H])[H] 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 238000010845 search algorithm Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83973506P | 2006-08-23 | 2006-08-23 | |
| US90324707P | 2007-02-23 | 2007-02-23 | |
| PCT/US2007/018523 WO2008024372A2 (en) | 2006-08-23 | 2007-08-21 | Pullulanase variants with increased productivity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010501181A JP2010501181A (ja) | 2010-01-21 |
| JP2010501181A5 true JP2010501181A5 (https=) | 2013-03-14 |
Family
ID=38969548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009525612A Pending JP2010501181A (ja) | 2006-08-23 | 2007-08-21 | 増加した生産力を伴うプルラナーゼ変異体 |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7968691B2 (https=) |
| EP (1) | EP2054512B1 (https=) |
| JP (1) | JP2010501181A (https=) |
| KR (1) | KR20090041410A (https=) |
| CN (2) | CN101506360B (https=) |
| BR (1) | BRPI0715619A2 (https=) |
| CA (2) | CA2663948C (https=) |
| CO (1) | CO6160247A2 (https=) |
| EA (1) | EA017712B1 (https=) |
| ES (1) | ES2547857T3 (https=) |
| HU (1) | HUE025068T2 (https=) |
| MX (1) | MX2009001717A (https=) |
| PL (1) | PL2054512T3 (https=) |
| WO (1) | WO2008024372A2 (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7968691B2 (en) * | 2006-08-23 | 2011-06-28 | Danisco Us Inc. | Pullulanase variants with increased productivity |
| IN2012DN00427A (https=) | 2009-07-01 | 2015-05-15 | Bayer Bioscience Nv | |
| ES2676895T5 (es) | 2013-03-11 | 2022-04-27 | Danisco Us Inc | Variantes combinatorias de alfa-amilasa |
| EP3022300B1 (en) * | 2013-07-17 | 2018-07-11 | Novozymes A/S | Pullulanase chimeras and polynucleotides encoding same |
| BR112016009416A2 (pt) | 2013-10-28 | 2017-10-03 | Danisco Us Inc | Levedura seca ativa geneticamente modificada de grande escala |
| MX2016006489A (es) | 2013-11-20 | 2016-08-03 | Danisco Us Inc | Alfa-amilasas variantes que tienen susceptibilidad reducida a la escision por proteasas y metodos de uso. |
| WO2016062875A2 (en) | 2014-10-23 | 2016-04-28 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| CN108064306B (zh) | 2014-12-23 | 2022-11-01 | 营养与生物科学美国4公司 | 酶促产生的纤维素 |
| EP3253869A4 (en) * | 2015-02-04 | 2018-11-07 | Nanjing Bestzyme Bio-Engineering Co. Ltd. | Truncated pullulanases, methods of production, and methods of use thereof |
| US20180030456A1 (en) | 2015-02-19 | 2018-02-01 | Danisco Us Inc. | Enhanced protein expression |
| CN109071615A (zh) | 2016-03-04 | 2018-12-21 | 丹尼斯科美国公司 | 用于在微生物中产生蛋白质的工程化核糖体启动子 |
| CN105925594A (zh) * | 2016-06-13 | 2016-09-07 | 广西大学 | 生淀粉糖化酶及其制备方法与其在生淀粉水解和生料同步糖化发酵产酒精中的应用 |
| US20190367932A1 (en) | 2017-01-23 | 2019-12-05 | Danisco Us Inc. | Methods and compositions for obtaining natural comptence in bacillus host cells |
| JP7231228B2 (ja) | 2017-02-24 | 2023-03-01 | ダニスコ・ユーエス・インク | バチルス・リケニフォルミスにおける増加したタンパク質産生のための組成物及び方法 |
| EP3703661A1 (en) | 2017-11-02 | 2020-09-09 | Danisco US Inc. | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
| KR102715197B1 (ko) | 2018-01-03 | 2024-10-08 | 다니스코 유에스 인크. | 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법 |
| WO2020172465A1 (en) * | 2019-02-20 | 2020-08-27 | Duke University | Compositions and methods for the treatment of genetic diseases |
| CN110184258B (zh) * | 2019-06-11 | 2020-11-06 | 南京林业大学 | 一种普鲁兰酶突变体 |
| CN110791513B (zh) * | 2019-11-15 | 2021-05-25 | 中国科学院南海海洋研究所 | 一种密码子优化的玉足海参α-淀粉酶基因及其重组表达载体与应用 |
| CN111662933B (zh) * | 2020-06-24 | 2023-03-24 | 江南大学 | 一种利用重组酵母进行高支链淀粉酒精发酵的方法 |
| US12508305B2 (en) * | 2020-12-26 | 2025-12-30 | Duke University | Compositions and methods for treating and/or preventing glycogen storage disease type III |
| EP4387982A2 (en) * | 2021-08-20 | 2024-06-26 | Danisco US Inc. | Methods and compositions for enhanced protein production in bacillus cells |
| EP4525615A2 (en) | 2022-05-14 | 2025-03-26 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US5264366A (en) * | 1984-05-29 | 1993-11-23 | Genencor, Inc. | Protease deficient bacillus |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| DE3650202T3 (de) | 1985-08-29 | 2004-06-03 | Genencor International, Inc., South San Francisco | Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung. |
| DK0605040T3 (da) * | 1992-12-28 | 2000-03-13 | Genencor Int | Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6537776B1 (en) * | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
| JP4307563B2 (ja) * | 1997-04-07 | 2009-08-05 | ユニリーバー・ナームローゼ・ベンノートシャープ | 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換 |
| EP0981628A1 (en) * | 1997-05-06 | 2000-03-01 | E.I. Dupont De Nemours And Company | Corn pullulanase |
| JP2002505108A (ja) * | 1998-03-04 | 2002-02-19 | ジェネンコア インターナショナル インコーポレーテッド | プルラナーゼの修飾形態 |
| CN1415016B (zh) * | 1999-12-30 | 2012-07-11 | 金克克国际有限公司 | Trichoderma reesei木聚糖酶 |
| US6350599B1 (en) * | 2000-01-12 | 2002-02-26 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
| AU2663801A (en) * | 2000-01-12 | 2001-07-24 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
| US7886330B2 (en) | 2000-06-30 | 2011-02-08 | Jlb Ventures Llc | Enhanced home entertainment system with removable long-term storage |
| WO2002014490A2 (en) | 2000-08-11 | 2002-02-21 | Genencor International, Inc. | Bacillus transformation, transformants and mutant libraries |
| US7713725B2 (en) * | 2002-09-10 | 2010-05-11 | Danisco Us Inc. | Induction of gene expression using a high concentration sugar mixture |
| US7968691B2 (en) * | 2006-08-23 | 2011-06-28 | Danisco Us Inc. | Pullulanase variants with increased productivity |
-
2007
- 2007-08-20 US US11/841,526 patent/US7968691B2/en not_active Expired - Fee Related
- 2007-08-21 CN CN200780031317.XA patent/CN101506360B/zh not_active Expired - Fee Related
- 2007-08-21 BR BRPI0715619-7A patent/BRPI0715619A2/pt not_active Application Discontinuation
- 2007-08-21 HU HUE07837179A patent/HUE025068T2/en unknown
- 2007-08-21 MX MX2009001717A patent/MX2009001717A/es active IP Right Grant
- 2007-08-21 CN CN201410181518.XA patent/CN103898080B/zh not_active Expired - Fee Related
- 2007-08-21 EP EP20070837179 patent/EP2054512B1/en not_active Not-in-force
- 2007-08-21 CA CA2663948A patent/CA2663948C/en not_active Expired - Fee Related
- 2007-08-21 WO PCT/US2007/018523 patent/WO2008024372A2/en not_active Ceased
- 2007-08-21 CA CA2949536A patent/CA2949536A1/en not_active Abandoned
- 2007-08-21 PL PL07837179T patent/PL2054512T3/pl unknown
- 2007-08-21 JP JP2009525612A patent/JP2010501181A/ja active Pending
- 2007-08-21 ES ES07837179.6T patent/ES2547857T3/es active Active
- 2007-08-21 KR KR1020097003544A patent/KR20090041410A/ko not_active Abandoned
- 2007-08-21 EA EA200970212A patent/EA017712B1/ru not_active IP Right Cessation
-
2009
- 2009-02-20 CO CO09017236A patent/CO6160247A2/es unknown
-
2011
- 2011-02-28 US US13/036,749 patent/US8354101B2/en not_active Expired - Fee Related
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