JP2010501181A - 増加した生産力を伴うプルラナーゼ変異体 - Google Patents

増加した生産力を伴うプルラナーゼ変異体 Download PDF

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JP2010501181A
JP2010501181A JP2009525612A JP2009525612A JP2010501181A JP 2010501181 A JP2010501181 A JP 2010501181A JP 2009525612 A JP2009525612 A JP 2009525612A JP 2009525612 A JP2009525612 A JP 2009525612A JP 2010501181 A JP2010501181 A JP 2010501181A
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pullulanase
host organism
sequence
seq
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JP2010501181A5 (https=
Inventor
イングランド、ジョージ
コルクマン、マーク
ミラー、ブライアン・エス
ブローメン、キャスパー
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ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン
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Publication of JP2010501181A publication Critical patent/JP2010501181A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2009525612A 2006-08-23 2007-08-21 増加した生産力を伴うプルラナーゼ変異体 Pending JP2010501181A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US83973506P 2006-08-23 2006-08-23
US90324707P 2007-02-23 2007-02-23
PCT/US2007/018523 WO2008024372A2 (en) 2006-08-23 2007-08-21 Pullulanase variants with increased productivity

Publications (2)

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JP2010501181A true JP2010501181A (ja) 2010-01-21
JP2010501181A5 JP2010501181A5 (https=) 2013-03-14

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JP2009525612A Pending JP2010501181A (ja) 2006-08-23 2007-08-21 増加した生産力を伴うプルラナーゼ変異体

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US (2) US7968691B2 (https=)
EP (1) EP2054512B1 (https=)
JP (1) JP2010501181A (https=)
KR (1) KR20090041410A (https=)
CN (2) CN103898080B (https=)
BR (1) BRPI0715619A2 (https=)
CA (2) CA2663948C (https=)
CO (1) CO6160247A2 (https=)
EA (1) EA017712B1 (https=)
ES (1) ES2547857T3 (https=)
HU (1) HUE025068T2 (https=)
MX (1) MX2009001717A (https=)
PL (1) PL2054512T3 (https=)
WO (1) WO2008024372A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018504136A (ja) * 2015-02-04 2018-02-15 ナンジン ベストザイム バイオ−エンジニアリング コンパニー リミテッド 切断型プルラナーゼの製造および使用方法

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US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
US20120096600A1 (en) 2009-07-01 2012-04-19 Rene Ruiter Methods and means for obtaining plants with enhanced glyphosate tolerance
DK2970931T3 (en) 2013-03-11 2018-01-22 Danisco Us Inc COMBINATORY ALPHA AMYLASE VARIATIONS
HUE040545T2 (hu) 2013-07-17 2019-03-28 Novozymes As Pullulanáz kimérák és az azokat kódoló polinukleotidok
BR112016009416A2 (pt) 2013-10-28 2017-10-03 Danisco Us Inc Levedura seca ativa geneticamente modificada de grande escala
US20160272957A1 (en) 2013-11-20 2016-09-22 Danisco Us Inc. Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
CN107109450A (zh) 2014-12-23 2017-08-29 纳幕尔杜邦公司 酶促产生的纤维素
EP3259358B1 (en) * 2015-02-19 2024-06-19 Danisco US Inc. Enhanced protein expression
WO2017152169A1 (en) 2016-03-04 2017-09-08 Danisco Us Inc. Engineered ribosomal promoters for protein production in microorganisms
CN105925594A (zh) * 2016-06-13 2016-09-07 广西大学 生淀粉糖化酶及其制备方法与其在生淀粉水解和生料同步糖化发酵产酒精中的应用
EP3571217B1 (en) 2017-01-23 2023-06-21 Danisco US Inc. Methods and compositions for obtaining natural competence in bacillus host cells
EP3585910B1 (en) 2017-02-24 2024-03-20 Danisco US Inc. Compositions and methods for increased protein production in bacillus licheniformis
EP3703661A1 (en) 2017-11-02 2020-09-09 Danisco US Inc. Freezing point depressed solid matrix compositions for melt granulation of enzymes
JP7280269B2 (ja) 2018-01-03 2023-05-23 ダニスコ・ユーエス・インク タンパク質の産生増加のための変異及び遺伝子改変バチルス属（ｂａｃｉｌｌｕｓ）細胞、並びにその方法
WO2020172465A1 (en) * 2019-02-20 2020-08-27 Duke University Compositions and methods for the treatment of genetic diseases
CN110184258B (zh) * 2019-06-11 2020-11-06 南京林业大学 一种普鲁兰酶突变体
CN110791513B (zh) * 2019-11-15 2021-05-25 中国科学院南海海洋研究所 一种密码子优化的玉足海参α-淀粉酶基因及其重组表达载体与应用
CN111662933B (zh) * 2020-06-24 2023-03-24 江南大学 一种利用重组酵母进行高支链淀粉酒精发酵的方法
US12508305B2 (en) * 2020-12-26 2025-12-30 Duke University Compositions and methods for treating and/or preventing glycogen storage disease type III
WO2023023642A2 (en) * 2021-08-20 2023-02-23 Danisco Us Inc. Methods and compositions for enhanced protein production in bacillus cells
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法

Citations (3)

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JPH06217770A (ja) * 1992-12-28 1994-08-09 Solvay & Cie プルラナーゼ、それを生産する微生物、そのプルラナーゼの調製方法及び使用
WO2001051620A2 (en) * 2000-01-12 2001-07-19 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
JP2002505108A (ja) * 1998-03-04 2002-02-19 ジェネンコア インターナショナル インコーポレーテッド プルラナーゼの修飾形態

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JPH06217770A (ja) * 1992-12-28 1994-08-09 Solvay & Cie プルラナーゼ、それを生産する微生物、そのプルラナーゼの調製方法及び使用
JP2002505108A (ja) * 1998-03-04 2002-02-19 ジェネンコア インターナショナル インコーポレーテッド プルラナーゼの修飾形態
WO2001051620A2 (en) * 2000-01-12 2001-07-19 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
JP2018504136A (ja) * 2015-02-04 2018-02-15 ナンジン ベストザイム バイオ−エンジニアリング コンパニー リミテッド 切断型プルラナーゼの製造および使用方法
US10400228B2 (en) 2015-02-04 2019-09-03 Nanjing Bestzyme Bio-Engineering Co., Ltd. Truncated pullulanases, methods of production, and methods of use thereof

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Publication number Publication date
CO6160247A2 (es) 2010-05-20
CN101506360B (zh) 2014-06-11
US8354101B2 (en) 2013-01-15
MX2009001717A (es) 2009-02-25
HK1135137A1 (en) 2010-05-28
US20110281326A1 (en) 2011-11-17
PL2054512T3 (pl) 2015-09-30
EA200970212A1 (ru) 2009-08-28
EA017712B1 (ru) 2013-02-28
CA2663948A1 (en) 2008-02-28
CA2949536A1 (en) 2008-02-28
EP2054512B1 (en) 2015-04-22
ES2547857T3 (es) 2015-10-09
CN103898080B (zh) 2017-04-12
BRPI0715619A2 (pt) 2013-06-25
WO2008024372A3 (en) 2008-04-24
EP2054512A2 (en) 2009-05-06
HUE025068T2 (en) 2016-01-28
CA2663948C (en) 2017-01-03
CN103898080A (zh) 2014-07-02
US7968691B2 (en) 2011-06-28
US20080108126A1 (en) 2008-05-08
KR20090041410A (ko) 2009-04-28
CN101506360A (zh) 2009-08-12
WO2008024372A2 (en) 2008-02-28

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