CN101506360B - 具有增加产率的支链淀粉酶变体 - Google Patents

具有增加产率的支链淀粉酶变体 Download PDF

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Publication number
CN101506360B
CN101506360B CN200780031317.XA CN200780031317A CN101506360B CN 101506360 B CN101506360 B CN 101506360B CN 200780031317 A CN200780031317 A CN 200780031317A CN 101506360 B CN101506360 B CN 101506360B
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pullulanase
host organism
seq
sequence
bacillus
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Chinese (zh)
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CN101506360A (zh
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G·英格兰德
M·孔克曼
B·S·米勒
C·弗勒门
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Danisco US Inc
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Danisco US Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN200780031317.XA 2006-08-23 2007-08-21 具有增加产率的支链淀粉酶变体 Expired - Fee Related CN101506360B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410181518.XA CN103898080B (zh) 2006-08-23 2007-08-21 具有增加产率的支链淀粉酶变体

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US83973506P 2006-08-23 2006-08-23
US60/839,735 2006-08-23
US90324707P 2007-02-23 2007-02-23
US60/903,247 2007-02-23
PCT/US2007/018523 WO2008024372A2 (en) 2006-08-23 2007-08-21 Pullulanase variants with increased productivity

Related Child Applications (1)

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CN201410181518.XA Division CN103898080B (zh) 2006-08-23 2007-08-21 具有增加产率的支链淀粉酶变体

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CN101506360A CN101506360A (zh) 2009-08-12
CN101506360B true CN101506360B (zh) 2014-06-11

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CN200780031317.XA Expired - Fee Related CN101506360B (zh) 2006-08-23 2007-08-21 具有增加产率的支链淀粉酶变体
CN201410181518.XA Expired - Fee Related CN103898080B (zh) 2006-08-23 2007-08-21 具有增加产率的支链淀粉酶变体

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US (2) US7968691B2 (https=)
EP (1) EP2054512B1 (https=)
JP (1) JP2010501181A (https=)
KR (1) KR20090041410A (https=)
CN (2) CN101506360B (https=)
BR (1) BRPI0715619A2 (https=)
CA (2) CA2663948C (https=)
CO (1) CO6160247A2 (https=)
EA (1) EA017712B1 (https=)
ES (1) ES2547857T3 (https=)
HU (1) HUE025068T2 (https=)
MX (1) MX2009001717A (https=)
PL (1) PL2054512T3 (https=)
WO (1) WO2008024372A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103898080A (zh) * 2006-08-23 2014-07-02 丹尼斯科美国公司 具有增加产率的支链淀粉酶变体

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2012DN00427A (https=) 2009-07-01 2015-05-15 Bayer Bioscience Nv
ES2676895T5 (es) 2013-03-11 2022-04-27 Danisco Us Inc Variantes combinatorias de alfa-amilasa
EP3022300B1 (en) * 2013-07-17 2018-07-11 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
BR112016009416A2 (pt) 2013-10-28 2017-10-03 Danisco Us Inc Levedura seca ativa geneticamente modificada de grande escala
MX2016006489A (es) 2013-11-20 2016-08-03 Danisco Us Inc Alfa-amilasas variantes que tienen susceptibilidad reducida a la escision por proteasas y metodos de uso.
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
CN108064306B (zh) 2014-12-23 2022-11-01 营养与生物科学美国4公司 酶促产生的纤维素
EP3253869A4 (en) * 2015-02-04 2018-11-07 Nanjing Bestzyme Bio-Engineering Co. Ltd. Truncated pullulanases, methods of production, and methods of use thereof
US20180030456A1 (en) 2015-02-19 2018-02-01 Danisco Us Inc. Enhanced protein expression
CN109071615A (zh) 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
CN105925594A (zh) * 2016-06-13 2016-09-07 广西大学 生淀粉糖化酶及其制备方法与其在生淀粉水解和生料同步糖化发酵产酒精中的应用
US20190367932A1 (en) 2017-01-23 2019-12-05 Danisco Us Inc. Methods and compositions for obtaining natural comptence in bacillus host cells
JP7231228B2 (ja) 2017-02-24 2023-03-01 ダニスコ・ユーエス・インク バチルス・リケニフォルミスにおける増加したタンパク質産生のための組成物及び方法
EP3703661A1 (en) 2017-11-02 2020-09-09 Danisco US Inc. Freezing point depressed solid matrix compositions for melt granulation of enzymes
KR102715197B1 (ko) 2018-01-03 2024-10-08 다니스코 유에스 인크. 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법
WO2020172465A1 (en) * 2019-02-20 2020-08-27 Duke University Compositions and methods for the treatment of genetic diseases
CN110184258B (zh) * 2019-06-11 2020-11-06 南京林业大学 一种普鲁兰酶突变体
CN110791513B (zh) * 2019-11-15 2021-05-25 中国科学院南海海洋研究所 一种密码子优化的玉足海参α-淀粉酶基因及其重组表达载体与应用
CN111662933B (zh) * 2020-06-24 2023-03-24 江南大学 一种利用重组酵母进行高支链淀粉酒精发酵的方法
US12508305B2 (en) * 2020-12-26 2025-12-30 Duke University Compositions and methods for treating and/or preventing glycogen storage disease type III
EP4387982A2 (en) * 2021-08-20 2024-06-26 Danisco US Inc. Methods and compositions for enhanced protein production in bacillus cells
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5264366A (en) * 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DE3650202T3 (de) 1985-08-29 2004-06-03 Genencor International, Inc., South San Francisco Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung.
DK0605040T3 (da) * 1992-12-28 2000-03-13 Genencor Int Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
JP4307563B2 (ja) * 1997-04-07 2009-08-05 ユニリーバー・ナームローゼ・ベンノートシャープ 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換
EP0981628A1 (en) * 1997-05-06 2000-03-01 E.I. Dupont De Nemours And Company Corn pullulanase
JP2002505108A (ja) * 1998-03-04 2002-02-19 ジェネンコア インターナショナル インコーポレーテッド プルラナーゼの修飾形態
CN1415016B (zh) * 1999-12-30 2012-07-11 金克克国际有限公司 Trichoderma reesei木聚糖酶
US6350599B1 (en) * 2000-01-12 2002-02-26 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
AU2663801A (en) * 2000-01-12 2001-07-24 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
US7886330B2 (en) 2000-06-30 2011-02-08 Jlb Ventures Llc Enhanced home entertainment system with removable long-term storage
WO2002014490A2 (en) 2000-08-11 2002-02-21 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
US7713725B2 (en) * 2002-09-10 2010-05-11 Danisco Us Inc. Induction of gene expression using a high concentration sugar mixture
US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Jorgensen L et al.Accession:AAA72993.《NCBI database》.1993, *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103898080A (zh) * 2006-08-23 2014-07-02 丹尼斯科美国公司 具有增加产率的支链淀粉酶变体

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KR20090041410A (ko) 2009-04-28
EA017712B1 (ru) 2013-02-28
US7968691B2 (en) 2011-06-28
JP2010501181A (ja) 2010-01-21
CO6160247A2 (es) 2010-05-20
WO2008024372A2 (en) 2008-02-28
EP2054512A2 (en) 2009-05-06
HK1135137A1 (en) 2010-05-28
BRPI0715619A2 (pt) 2013-06-25
CN103898080B (zh) 2017-04-12
US20080108126A1 (en) 2008-05-08
CA2663948C (en) 2017-01-03
US8354101B2 (en) 2013-01-15
CA2949536A1 (en) 2008-02-28
CN101506360A (zh) 2009-08-12
ES2547857T3 (es) 2015-10-09
EP2054512B1 (en) 2015-04-22
CA2663948A1 (en) 2008-02-28
HUE025068T2 (en) 2016-01-28
MX2009001717A (es) 2009-02-25
US20110281326A1 (en) 2011-11-17
PL2054512T3 (pl) 2015-09-30
EA200970212A1 (ru) 2009-08-28
WO2008024372A3 (en) 2008-04-24
CN103898080A (zh) 2014-07-02

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