KR20090041410A - 생산성이 증가된 풀루라나아제 변형체 - Google Patents

생산성이 증가된 풀루라나아제 변형체 Download PDF

Info

Publication number
KR20090041410A
KR20090041410A KR1020097003544A KR20097003544A KR20090041410A KR 20090041410 A KR20090041410 A KR 20090041410A KR 1020097003544 A KR1020097003544 A KR 1020097003544A KR 20097003544 A KR20097003544 A KR 20097003544A KR 20090041410 A KR20090041410 A KR 20090041410A
Authority
KR
South Korea
Prior art keywords
pullulanase
asp
ala
gly
thr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
KR1020097003544A
Other languages
English (en)
Korean (ko)
Inventor
조지 잉글랜드
마르크 콜크만
브라이언 에스 밀러
카스퍼 브뢰멘
Original Assignee
다니스코 유에스 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 다니스코 유에스 인크. filed Critical 다니스코 유에스 인크.
Publication of KR20090041410A publication Critical patent/KR20090041410A/ko
Abandoned legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020097003544A 2006-08-23 2007-08-21 생산성이 증가된 풀루라나아제 변형체 Abandoned KR20090041410A (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US83973506P 2006-08-23 2006-08-23
US60/839,735 2006-08-23
US90324707P 2007-02-23 2007-02-23
US60/903,247 2007-02-23

Publications (1)

Publication Number Publication Date
KR20090041410A true KR20090041410A (ko) 2009-04-28

Family

ID=38969548

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020097003544A Abandoned KR20090041410A (ko) 2006-08-23 2007-08-21 생산성이 증가된 풀루라나아제 변형체

Country Status (14)

Country Link
US (2) US7968691B2 (https=)
EP (1) EP2054512B1 (https=)
JP (1) JP2010501181A (https=)
KR (1) KR20090041410A (https=)
CN (2) CN103898080B (https=)
BR (1) BRPI0715619A2 (https=)
CA (2) CA2663948C (https=)
CO (1) CO6160247A2 (https=)
EA (1) EA017712B1 (https=)
ES (1) ES2547857T3 (https=)
HU (1) HUE025068T2 (https=)
MX (1) MX2009001717A (https=)
PL (1) PL2054512T3 (https=)
WO (1) WO2008024372A2 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
US20120096600A1 (en) 2009-07-01 2012-04-19 Rene Ruiter Methods and means for obtaining plants with enhanced glyphosate tolerance
DK2970931T3 (en) 2013-03-11 2018-01-22 Danisco Us Inc COMBINATORY ALPHA AMYLASE VARIATIONS
HUE040545T2 (hu) 2013-07-17 2019-03-28 Novozymes As Pullulanáz kimérák és az azokat kódoló polinukleotidok
BR112016009416A2 (pt) 2013-10-28 2017-10-03 Danisco Us Inc Levedura seca ativa geneticamente modificada de grande escala
US20160272957A1 (en) 2013-11-20 2016-09-22 Danisco Us Inc. Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
CN107109450A (zh) 2014-12-23 2017-08-29 纳幕尔杜邦公司 酶促产生的纤维素
JP6505240B2 (ja) * 2015-02-04 2019-04-24 ナンジン ベストザイム バイオ−エンジニアリング コンパニー リミテッド 切断型プルラナーゼの製造および使用方法
EP3259358B1 (en) * 2015-02-19 2024-06-19 Danisco US Inc. Enhanced protein expression
WO2017152169A1 (en) 2016-03-04 2017-09-08 Danisco Us Inc. Engineered ribosomal promoters for protein production in microorganisms
CN105925594A (zh) * 2016-06-13 2016-09-07 广西大学 生淀粉糖化酶及其制备方法与其在生淀粉水解和生料同步糖化发酵产酒精中的应用
EP3571217B1 (en) 2017-01-23 2023-06-21 Danisco US Inc. Methods and compositions for obtaining natural competence in bacillus host cells
EP3585910B1 (en) 2017-02-24 2024-03-20 Danisco US Inc. Compositions and methods for increased protein production in bacillus licheniformis
EP3703661A1 (en) 2017-11-02 2020-09-09 Danisco US Inc. Freezing point depressed solid matrix compositions for melt granulation of enzymes
JP7280269B2 (ja) 2018-01-03 2023-05-23 ダニスコ・ユーエス・インク タンパク質の産生増加のための変異及び遺伝子改変バチルス属（ｂａｃｉｌｌｕｓ）細胞、並びにその方法
WO2020172465A1 (en) * 2019-02-20 2020-08-27 Duke University Compositions and methods for the treatment of genetic diseases
CN110184258B (zh) * 2019-06-11 2020-11-06 南京林业大学 一种普鲁兰酶突变体
CN110791513B (zh) * 2019-11-15 2021-05-25 中国科学院南海海洋研究所 一种密码子优化的玉足海参α-淀粉酶基因及其重组表达载体与应用
CN111662933B (zh) * 2020-06-24 2023-03-24 江南大学 一种利用重组酵母进行高支链淀粉酒精发酵的方法
US12508305B2 (en) * 2020-12-26 2025-12-30 Duke University Compositions and methods for treating and/or preventing glycogen storage disease type III
WO2023023642A2 (en) * 2021-08-20 2023-02-23 Danisco Us Inc. Methods and compositions for enhanced protein production in bacillus cells
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DE3650202T3 (de) 1985-08-29 2004-06-03 Genencor International, Inc., South San Francisco Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung.
EP0605040B1 (fr) * 1992-12-28 1999-08-11 Genencor International, Inc. Pullulanase, microorganismes la produisant, procédés de préparation de cette pullulanase et utilisations de celle-ci
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6255115B1 (en) 1997-04-07 2001-07-03 Unilever Patent Holdings Bv Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus
CA2284688C (en) 1997-05-06 2010-02-16 E.I. Du Pont De Nemours And Company Corn pullulanase
BR9908422A (pt) * 1998-03-04 2000-10-31 Genencor Int Pululanase modificada, ácido nucleico, vetor de expressão, microorganismo hospedeiro, processo para a produção de uma pululanase modificada em uma célula hospedeira, composição enzimática, processo para sacarificação de amido, e, b. licheniformis
ATE288493T1 (de) 1999-12-30 2005-02-15 Genencor Int Trichoderma reesei xylanase
US6350599B1 (en) 2000-01-12 2002-02-26 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
EP1250423B1 (en) 2000-01-12 2008-09-03 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
US7886330B2 (en) 2000-06-30 2011-02-08 Jlb Ventures Llc Enhanced home entertainment system with removable long-term storage
CA2418317A1 (en) 2000-08-11 2002-02-21 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
CA2498213C (en) 2002-09-10 2013-07-16 Genencor International, Inc. Induction of gene expression using a high concentration sugar mixture
US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity

Also Published As

Publication number Publication date
CO6160247A2 (es) 2010-05-20
JP2010501181A (ja) 2010-01-21
CN101506360B (zh) 2014-06-11
US8354101B2 (en) 2013-01-15
MX2009001717A (es) 2009-02-25
HK1135137A1 (en) 2010-05-28
US20110281326A1 (en) 2011-11-17
PL2054512T3 (pl) 2015-09-30
EA200970212A1 (ru) 2009-08-28
EA017712B1 (ru) 2013-02-28
CA2663948A1 (en) 2008-02-28
CA2949536A1 (en) 2008-02-28
EP2054512B1 (en) 2015-04-22
ES2547857T3 (es) 2015-10-09
CN103898080B (zh) 2017-04-12
BRPI0715619A2 (pt) 2013-06-25
WO2008024372A3 (en) 2008-04-24
EP2054512A2 (en) 2009-05-06
HUE025068T2 (en) 2016-01-28
CA2663948C (en) 2017-01-03
CN103898080A (zh) 2014-07-02
US7968691B2 (en) 2011-06-28
US20080108126A1 (en) 2008-05-08
CN101506360A (zh) 2009-08-12
WO2008024372A2 (en) 2008-02-28

Similar Documents

Publication Publication Date Title
KR20090041410A (ko) 생산성이 증가된 풀루라나아제 변형체
KR102375732B1 (ko) 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법
JP4922524B2 (ja) 糸状菌の分野における新規発現調節配列および発現産物
US8741654B2 (en) Compositions and methods for improved protein production
EP3104717B1 (en) Sucrose reduction and generation of insoluble fiber in juices
EP3380496B1 (en) Enhanced protein production and methods thereof
US11377647B2 (en) Amylase mutant having high specific activity and thermal stability, gene of mutant, and applications thereof
ES2703753T3 (es) Procedimiento de expresión
CN107250365A (zh) 增强的蛋白质表达
KR20180117166A (ko) 미생물에서의 단백질 생산을 위한 유전자 조작 리보솜 프로모터
CN120500529A (zh) 折叠酶用于改善分泌分子的异源表达的用途
Yoshino-Yasuda et al. Sequence analysis and heterologous expression of rhamnogalacturonan lyase A gene (AsrglA) from Shoyu Koji mold, Aspergillus sojae KBN1340
HK1135137B (en) Pullulanase variants with increased productivity
WO2008060596A9 (en) Enhanced protein production in t. reesei by co-expression of selected genes
JPWO2018123856A1 (ja) 真菌におけるタンパク質の選択的分泌技術
JP2010284130A (ja) バチルス属細菌由来の改変プロモーター

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20090220

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20120809

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20131126

Patent event code: PE09021S01D

E90F Notification of reason for final refusal
PE0902 Notice of grounds for rejection

Comment text: Final Notice of Reason for Refusal

Patent event date: 20140527

Patent event code: PE09021S02D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20141125

NORF Unpaid initial registration fee
PC1904 Unpaid initial registration fee